Click here to switch to the map view.

The map label for this gene is chrR [H]

Identifier: 152975135

GI number: 152975135

Start: 1440314

End: 1441048

Strand: Direct

Name: chrR [H]

Synonym: Bcer98_1336

Alternate gene names: 152975135

Gene position: 1440314-1441048 (Clockwise)

Preceding gene: 152975134

Following gene: 152975137

Centisome position: 35.24

GC content: 36.46

Gene sequence:

>735_bases
ATGAATGAAATGATACAGAAAATGAAGAGTCATACGTCAGTACGAAAATATAAAAAGGAATCGGTTCCTAAAAATATAAT
AGAACGAATGGTACAGGCAGCTCAGCATGCAGCATCATCTCATTTTGTTCAAGCGTATTCCGTTCTCTATGTAACAGATG
AAACATTAAAAGTAAAGCTAGCAGAGCAATCTGGAAATCGACATGTGCAACATTGCGGAGCATTCGTTGTTTGTTGTGCC
GATTTAAAACGTTTAGAAATTGCTTGTGAGAAACATGGAGCAGAGATCAAGCACGATAGCGTAGAAGATTTTATCGTTGC
TACAGTAGATGTATCATTATTTGCTCAAAACTTAGCGCCTGCTGCAGAATCATTGGGATATGGTATTTGTTATATTGGGG
GTATTCGAAATAACCCAGAAGAAGTGAGTGAGTTGCTTCATTTACCAGATAAAGTATATCCAGTCTTTGGGATGACAATT
GGAGTGCCAGATCAAGAACATGAGGTAAAACCGCGCTTGCCAGTCGAAGCCATTTTACATGAAAATACATACGATGTAGC
GAAATATGAATCATTATTAGAAAAATATGACGAAACGATGAGTGCATATTATAGTGCTCGATCTAGCAATCAAAAAAATG
TAACATGGACAGAATCGATGAGCACATTCATGTCAAACGAAAAACGAATGCATATGAAGGAATTTTTAAATAAAAGGGGT
TTAAATAAAAGGTAG

Upstream 100 bases:

>100_bases
AATATAATACCTTATTATGATGTCACTTTACTTGGAAGGAAAAAATAGTTTTTGCTACAATTTAAGTGTTAATTTTTCAT
TAGGAAGAGGGGCGATTTTG

Downstream 100 bases:

>100_bases
TGTGATAAGAGCTTACCTCAAAAGTATAGATTTTGAGATAGGCTCTTTTTATAAGTGCTAGTCCTATTTCTATTTTGGGT
AGCGGAGAAATAAGAAAAAA

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MNEMIQKMKSHTSVRKYKKESVPKNIIERMVQAAQHAASSHFVQAYSVLYVTDETLKVKLAEQSGNRHVQHCGAFVVCCA
DLKRLEIACEKHGAEIKHDSVEDFIVATVDVSLFAQNLAPAAESLGYGICYIGGIRNNPEEVSELLHLPDKVYPVFGMTI
GVPDQEHEVKPRLPVEAILHENTYDVAKYESLLEKYDETMSAYYSARSSNQKNVTWTESMSTFMSNEKRMHMKEFLNKRG
LNKR

Sequences:

>Translated_244_residues
MNEMIQKMKSHTSVRKYKKESVPKNIIERMVQAAQHAASSHFVQAYSVLYVTDETLKVKLAEQSGNRHVQHCGAFVVCCA
DLKRLEIACEKHGAEIKHDSVEDFIVATVDVSLFAQNLAPAAESLGYGICYIGGIRNNPEEVSELLHLPDKVYPVFGMTI
GVPDQEHEVKPRLPVEAILHENTYDVAKYESLLEKYDETMSAYYSARSSNQKNVTWTESMSTFMSNEKRMHMKEFLNKRG
LNKR
>Mature_244_residues
MNEMIQKMKSHTSVRKYKKESVPKNIIERMVQAAQHAASSHFVQAYSVLYVTDETLKVKLAEQSGNRHVQHCGAFVVCCA
DLKRLEIACEKHGAEIKHDSVEDFIVATVDVSLFAQNLAPAAESLGYGICYIGGIRNNPEEVSELLHLPDKVYPVFGMTI
GVPDQEHEVKPRLPVEAILHENTYDVAKYESLLEKYDETMSAYYSARSSNQKNVTWTESMSTFMSNEKRMHMKEFLNKRG
LNKR

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=245, Percent_Identity=34.6938775510204, Blast_Score=150, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 27732; Mature: 27732

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEMIQKMKSHTSVRKYKKESVPKNIIERMVQAAQHAASSHFVQAYSVLYVTDETLKVKL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEE
AEQSGNRHVQHCGAFVVCCADLKRLEIACEKHGAEIKHDSVEDFIVATVDVSLFAQNLAP
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAESLGYGICYIGGIRNNPEEVSELLHLPDKVYPVFGMTIGVPDQEHEVKPRLPVEAILH
HHHHCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHCEEECCCCCCCCCCCCCCHHHHHC
ENTYDVAKYESLLEKYDETMSAYYSARSSNQKNVTWTESMSTFMSNEKRMHMKEFLNKRG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCC
LNKR
CCCC
>Mature Secondary Structure
MNEMIQKMKSHTSVRKYKKESVPKNIIERMVQAAQHAASSHFVQAYSVLYVTDETLKVKL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEE
AEQSGNRHVQHCGAFVVCCADLKRLEIACEKHGAEIKHDSVEDFIVATVDVSLFAQNLAP
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAESLGYGICYIGGIRNNPEEVSELLHLPDKVYPVFGMTIGVPDQEHEVKPRLPVEAILH
HHHHCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHCEEECCCCCCCCCCCCCCHHHHHC
ENTYDVAKYESLLEKYDETMSAYYSARSSNQKNVTWTESMSTFMSNEKRMHMKEFLNKRG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCC
LNKR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA