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The map label for this gene is rnjA [H]

Identifier: 152975132

GI number: 152975132

Start: 1437017

End: 1438690

Strand: Direct

Name: rnjA [H]

Synonym: Bcer98_1333

Alternate gene names: 152975132

Gene position: 1437017-1438690 (Clockwise)

Preceding gene: 152975130

Following gene: 152975134

Centisome position: 35.16

GC content: 37.28

Gene sequence:

>1674_bases
TTGAGCATGTCAGAAAATACATTATCAATTTTTGCGTTAGGCGGAGTGAATGAGATAGGGAAAAATATGTATGCCCTTCA
ATATAAAAATGAGATTGTAGTAGTAGATTGTGGTTCTAAATTTCCAGATGAAAGCTTATTAGGCATTGACCTTATTATTC
CAGATATTACATATTTACAAGAAAATAAAGAAAAGATTCGAGGACTCTTTGTTACACATGGACATGAAGATCATATTGGG
GGAATACCGTATATATTAAAACAATTAAACATCCCCATTTATGCAAGCAAATTGACGCTCGGCTTAATCGAACTGAAATT
AAAAGAACATCACTTACAAAATCAAACAAAACTGATTATGGTTGACTCTGAATCTGAAATTGAGTTGGAGACAATGCGTC
TTACTTTTTTTAAAACAAATCATAGTATTCCTGATTGTCTCGGGATTGCATTTCATACAGATGAAGGAACAGTCGTACAT
ACAGGGGACTTTAAATTTGATTTAACACCTGTAAATGAGCAATATCCGGATATTCATAAAATGGCGAATATTGGGGAGAA
AGGAGTACTAGCGTTACTCTCTGAAAGTACAAATGCGGAGCGAGCCGGATTTAGCCCCTCAGAGCGCTTGGTAGGTGAAC
GAATAGAAGAAGCGTTTCAGAAAGCGCCAAGAAAAGTGATCCTCTCTACTTTTGCTTCAAATGTGAATCGTGTGCAACAA
GTAGTGAAAGCTTGTTTAAAAACAAAAAGGAAATTGGCGTTACTAGGAAGAAGTATGGTAAATGTAGTATCTGTCGCAAT
GGAGCAAGGATATTTAACGATTCCTGAAGGAATGTTAATTGAAGCGGATGAGATCAATAGGTTAGATCCAGAGAAAGTAG
CAATTCTCTGTACAGGCAGCCAAGGGGAACCGATGGCAGCACTTGCTCGTTTAGCTAGTGGAAGTTATCGGCAAGTGGAA
ATTTTGCCGGAGGATACGGTCATTATTGCGGCAACGCCTATACCTGGAAATGAACGTAACGTGTCACGTATTATCGATAA
TTTATTTGTATTAGGTGCCAATGTGATTTACGGTACGGGAAGTTCGACAGGTATGCATGTGTCCGGGCATGCATATCAGG
AAGAATTAAAACTAATGTTAACTTTAATGAAACCGAAATATTTCATTCCAATTCATGGTGAATTTAGAATGTTACACCAT
CATAGTTTGTTAGCGGAGTCTGTTGGGGTTCAAAAAGAGAACATTTTTATCATTAAAAATGGGGATGTAGTGGATATAAG
GAAACAAGTCGCCTATCAATCAAGAAGGATACCTGCTGGGAACATATATGTAGATGGACTGGGAATTGGTGATGTAGGAA
ATGCTCTTTTGCGTGATCGCAAACAACTTTCGGAAGATGGTATGCTTATTATTGTAATTACTTTGAGCAAAACAGACGGA
GATATGATTTCAGAACCAGACATTATTTCACGCGGTTTCATATATGTTCGTGATTCAGAAGAATTTCTGAAGGAGATTAA
TCAATCCGTTGTAGAAACGATTCAGCGCCTGCAGCAATCTTATACGGGCCAATGGTCGATTTTGAAAAAGGAAATAAGAG
AAAAGTTAGGTCAATTCATATATACGAGTACAAAACGAAAACCAATGATTCTGCCGATTATTATTGAAGTGTAA

Upstream 100 bases:

>100_bases
TTTCATAGCAGATAATAACCCTTAAATGGAATGGTTTTATAAAAAGGATACTATGCTTATGTAGATATCCTTTAATCGGT
AGAAAGGGAGGATATTGAAT

Downstream 100 bases:

>100_bases
TGAATTGTGGAGAAACTGAATCCCGCTTTCAAAGGAGGAGAGGGGAATATAGGAAAAGAATGCAGCATAATAGAAATCCC
CTTTTTTCTATATTAAGGGG

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MSMSENTLSIFALGGVNEIGKNMYALQYKNEIVVVDCGSKFPDESLLGIDLIIPDITYLQENKEKIRGLFVTHGHEDHIG
GIPYILKQLNIPIYASKLTLGLIELKLKEHHLQNQTKLIMVDSESEIELETMRLTFFKTNHSIPDCLGIAFHTDEGTVVH
TGDFKFDLTPVNEQYPDIHKMANIGEKGVLALLSESTNAERAGFSPSERLVGERIEEAFQKAPRKVILSTFASNVNRVQQ
VVKACLKTKRKLALLGRSMVNVVSVAMEQGYLTIPEGMLIEADEINRLDPEKVAILCTGSQGEPMAALARLASGSYRQVE
ILPEDTVIIAATPIPGNERNVSRIIDNLFVLGANVIYGTGSSTGMHVSGHAYQEELKLMLTLMKPKYFIPIHGEFRMLHH
HSLLAESVGVQKENIFIIKNGDVVDIRKQVAYQSRRIPAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLIIVITLSKTDG
DMISEPDIISRGFIYVRDSEEFLKEINQSVVETIQRLQQSYTGQWSILKKEIREKLGQFIYTSTKRKPMILPIIIEV

Sequences:

>Translated_557_residues
MSMSENTLSIFALGGVNEIGKNMYALQYKNEIVVVDCGSKFPDESLLGIDLIIPDITYLQENKEKIRGLFVTHGHEDHIG
GIPYILKQLNIPIYASKLTLGLIELKLKEHHLQNQTKLIMVDSESEIELETMRLTFFKTNHSIPDCLGIAFHTDEGTVVH
TGDFKFDLTPVNEQYPDIHKMANIGEKGVLALLSESTNAERAGFSPSERLVGERIEEAFQKAPRKVILSTFASNVNRVQQ
VVKACLKTKRKLALLGRSMVNVVSVAMEQGYLTIPEGMLIEADEINRLDPEKVAILCTGSQGEPMAALARLASGSYRQVE
ILPEDTVIIAATPIPGNERNVSRIIDNLFVLGANVIYGTGSSTGMHVSGHAYQEELKLMLTLMKPKYFIPIHGEFRMLHH
HSLLAESVGVQKENIFIIKNGDVVDIRKQVAYQSRRIPAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLIIVITLSKTDG
DMISEPDIISRGFIYVRDSEEFLKEINQSVVETIQRLQQSYTGQWSILKKEIREKLGQFIYTSTKRKPMILPIIIEV
>Mature_556_residues
SMSENTLSIFALGGVNEIGKNMYALQYKNEIVVVDCGSKFPDESLLGIDLIIPDITYLQENKEKIRGLFVTHGHEDHIGG
IPYILKQLNIPIYASKLTLGLIELKLKEHHLQNQTKLIMVDSESEIELETMRLTFFKTNHSIPDCLGIAFHTDEGTVVHT
GDFKFDLTPVNEQYPDIHKMANIGEKGVLALLSESTNAERAGFSPSERLVGERIEEAFQKAPRKVILSTFASNVNRVQQV
VKACLKTKRKLALLGRSMVNVVSVAMEQGYLTIPEGMLIEADEINRLDPEKVAILCTGSQGEPMAALARLASGSYRQVEI
LPEDTVIIAATPIPGNERNVSRIIDNLFVLGANVIYGTGSSTGMHVSGHAYQEELKLMLTLMKPKYFIPIHGEFRMLHHH
SLLAESVGVQKENIFIIKNGDVVDIRKQVAYQSRRIPAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLIIVITLSKTDGD
MISEPDIISRGFIYVRDSEEFLKEINQSVVETIQRLQQSYTGQWSILKKEIREKLGQFIYTSTKRKPMILPIIIEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 62200; Mature: 62069

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMSENTLSIFALGGVNEIGKNMYALQYKNEIVVVDCGSKFPDESLLGIDLIIPDITYLQ
CCCCCCEEEEEEECCHHHHCCCEEEEEECCEEEEEECCCCCCCCCCEEEEEECCCHHHHH
ENKEKIRGLFVTHGHEDHIGGIPYILKQLNIPIYASKLTLGLIELKLKEHHLQNQTKLIM
CCHHHHCEEEEECCCCCCCCCHHHHHHHCCCCEEEHHHEEEHEEEHHHHHHCCCCEEEEE
VDSESEIELETMRLTFFKTNHSIPDCLGIAFHTDEGTVVHTGDFKFDLTPVNEQYPDIHK
EECCCCEEEEEEEEEEEECCCCCCCCEEEEEECCCCCEEECCCEEEEEECCCCCCCCHHH
MANIGEKGVLALLSESTNAERAGFSPSERLVGERIEEAFQKAPRKVILSTFASNVNRVQQ
HHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
VVKACLKTKRKLALLGRSMVNVVSVAMEQGYLTIPEGMLIEADEINRLDPEKVAILCTGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEHHHCCCCCCCCEEEEEECC
QGEPMAALARLASGSYRQVEILPEDTVIIAATPIPGNERNVSRIIDNLFVLGANVIYGTG
CCCHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHEECEEEEECC
SSTGMHVSGHAYQEELKLMLTLMKPKYFIPIHGEFRMLHHHSLLAESVGVQKENIFIIKN
CCCCEEECCHHHHHHHHHHHHHHCCCEEEEECCCEEEHHHHHHHHHHHCCCCCCEEEEEC
GDVVDIRKQVAYQSRRIPAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLIIVITLSKTDG
CCEEHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
DMISEPDIISRGFIYVRDSEEFLKEINQSVVETIQRLQQSYTGQWSILKKEIREKLGQFI
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHE
YTSTKRKPMILPIIIEV
EECCCCCCEEEEEEEEC
>Mature Secondary Structure 
SMSENTLSIFALGGVNEIGKNMYALQYKNEIVVVDCGSKFPDESLLGIDLIIPDITYLQ
CCCCCEEEEEEECCHHHHCCCEEEEEECCEEEEEECCCCCCCCCCEEEEEECCCHHHHH
ENKEKIRGLFVTHGHEDHIGGIPYILKQLNIPIYASKLTLGLIELKLKEHHLQNQTKLIM
CCHHHHCEEEEECCCCCCCCCHHHHHHHCCCCEEEHHHEEEHEEEHHHHHHCCCCEEEEE
VDSESEIELETMRLTFFKTNHSIPDCLGIAFHTDEGTVVHTGDFKFDLTPVNEQYPDIHK
EECCCCEEEEEEEEEEEECCCCCCCCEEEEEECCCCCEEECCCEEEEEECCCCCCCCHHH
MANIGEKGVLALLSESTNAERAGFSPSERLVGERIEEAFQKAPRKVILSTFASNVNRVQQ
HHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
VVKACLKTKRKLALLGRSMVNVVSVAMEQGYLTIPEGMLIEADEINRLDPEKVAILCTGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEHHHCCCCCCCCEEEEEECC
QGEPMAALARLASGSYRQVEILPEDTVIIAATPIPGNERNVSRIIDNLFVLGANVIYGTG
CCCHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHEECEEEEECC
SSTGMHVSGHAYQEELKLMLTLMKPKYFIPIHGEFRMLHHHSLLAESVGVQKENIFIIKN
CCCCEEECCHHHHHHHHHHHHHHCCCEEEEECCCEEEHHHHHHHHHHHCCCCCCEEEEEC
GDVVDIRKQVAYQSRRIPAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLIIVITLSKTDG
CCEEHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
DMISEPDIISRGFIYVRDSEEFLKEINQSVVETIQRLQQSYTGQWSILKKEIREKLGQFI
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHE
YTSTKRKPMILPIIIEV
EECCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]