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The map label for this gene is rluB [H]

Identifier: 152974996

GI number: 152974996

Start: 1309686

End: 1310414

Strand: Direct

Name: rluB [H]

Synonym: Bcer98_1196

Alternate gene names: 152974996

Gene position: 1309686-1310414 (Clockwise)

Preceding gene: 152974995

Following gene: 152974997

Centisome position: 32.04

GC content: 39.64

Gene sequence:

>729_bases
ATGGAACGACTACAAAAAGTGATTGCACAAGCAGGTATTGCATCAAGAAGAAAGGCTGAGGAGCTTATTCAACAAGGGAA
AGTGAAAGTTAACGGAAAAATTGTGACCGAATTAGGCACAAAGGTAACTCCTCAAGATAAAGTAGAAGTAAATCATATTC
CTCTTGAGAAAGAAGAGCCTGTTTATTTCTTATTATATAAACCGACAGGAGTTATTTCGAGTGTATCAGATGATAAAGGA
CGAAAGGTTGTTACAGACTTTTTCCAAGATATCCCGCAACGTCTATTTCCGATTGGGCGTTTAGACTATGATACATCCGG
TGTGTTATTGATGACAAATGATGGTGACTTTGCAAATATATTAATGCATCCAAAGTACAAGGTGGAAAAAACATATGTTG
CAAAGGTAAAAGGTCCGCTGACAGGCGAAAAGATTCGCATGTTGGAAAAAGGCGTTATATTAGAAGATGGAAAAACATCA
CCGGCACGTGTGAAAATTATTTCTTGGGATAAGCGTAAAGAAATGTCAATTGTACAATTGACAATTCATGAAGGACGCAA
TCGTCAAGTTCGCCGTATGTTTGAGGCGATTGGCTGTAAAGTCATGAAGTTAAAGCGTGAACGATACGCTTTCTTAGAAG
TAGGTAGCTTGCGCCCTGGTGACGCACGAGAATTGACGCCGCATGAAGTGAAACAACTGCGTGCATTGGCATCTACAAAG
CCAAGATAA

Upstream 100 bases:

>100_bases
CTATGTTGTTTGGAGAAAACTAGAGTAAATCGCTACAAGACTTGGGCGGTTGCCAAGTGTTTCTAATGAAGGTAAGATAA
ATTTGAGGTGAAAAAAGGAA

Downstream 100 bases:

>100_bases
CGAATCACCAGTGGATTGTTCCACTGGTGATTCGTTACATGAGACCATTTTGTGAAAATAAGTGGGAGCCTTACTGACCA
TAACAACCCGATTGGCGAGG

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MERLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVTELGTKVTPQDKVEVNHIPLEKEEPVYFLLYKPTGVISSVSDDKG
RKVVTDFFQDIPQRLFPIGRLDYDTSGVLLMTNDGDFANILMHPKYKVEKTYVAKVKGPLTGEKIRMLEKGVILEDGKTS
PARVKIISWDKRKEMSIVQLTIHEGRNRQVRRMFEAIGCKVMKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
PR

Sequences:

>Translated_242_residues
MERLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVTELGTKVTPQDKVEVNHIPLEKEEPVYFLLYKPTGVISSVSDDKG
RKVVTDFFQDIPQRLFPIGRLDYDTSGVLLMTNDGDFANILMHPKYKVEKTYVAKVKGPLTGEKIRMLEKGVILEDGKTS
PARVKIISWDKRKEMSIVQLTIHEGRNRQVRRMFEAIGCKVMKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
PR
>Mature_242_residues
MERLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVTELGTKVTPQDKVEVNHIPLEKEEPVYFLLYKPTGVISSVSDDKG
RKVVTDFFQDIPQRLFPIGRLDYDTSGVLLMTNDGDFANILMHPKYKVEKTYVAKVKGPLTGEKIRMLEKGVILEDGKTS
PARVKIISWDKRKEMSIVQLTIHEGRNRQVRRMFEAIGCKVMKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
PR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=250, Percent_Identity=39.6, Blast_Score=143, Evalue=9e-36,
Organism=Escherichia coli, GI1788510, Length=235, Percent_Identity=35.7446808510638, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790453, Length=238, Percent_Identity=37.8151260504202, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI87081838, Length=185, Percent_Identity=33.5135135135135, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27539; Mature: 27539

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVTELGTKVTPQDKVEVNHIPLEKEEP
CHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCEEEEECCCCCCCCCCEEEECCCCCCCCC
VYFLLYKPTGVISSVSDDKGRKVVTDFFQDIPQRLFPIGRLDYDTSGVLLMTNDGDFANI
EEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEE
LMHPKYKVEKTYVAKVKGPLTGEKIRMLEKGVILEDGKTSPARVKIISWDKRKEMSIVQL
EECCCEEECCEEEEEECCCCCHHHHHHHHCCEEEECCCCCCCEEEEEECCCCCCCEEEEE
TIHEGRNRQVRRMFEAIGCKVMKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
EEECCCCHHHHHHHHHHCHHHEEHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
PR
CC
>Mature Secondary Structure
MERLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVTELGTKVTPQDKVEVNHIPLEKEEP
CHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCEEEEECCCCCCCCCCEEEECCCCCCCCC
VYFLLYKPTGVISSVSDDKGRKVVTDFFQDIPQRLFPIGRLDYDTSGVLLMTNDGDFANI
EEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEE
LMHPKYKVEKTYVAKVKGPLTGEKIRMLEKGVILEDGKTSPARVKIISWDKRKEMSIVQL
EECCCEEECCEEEEEECCCCCHHHHHHHHCCEEEECCCCCCCEEEEEECCCCCCCEEEEE
TIHEGRNRQVRRMFEAIGCKVMKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
EEECCCCHHHHHHHHHHCHHHEEHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
PR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]