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The map label for this gene is dacB [H]

Identifier: 152974993

GI number: 152974993

Start: 1307160

End: 1308281

Strand: Direct

Name: dacB [H]

Synonym: Bcer98_1193

Alternate gene names: 152974993

Gene position: 1307160-1308281 (Clockwise)

Preceding gene: 152974992

Following gene: 152974994

Centisome position: 31.98

GC content: 35.47

Gene sequence:

>1122_bases
ATGAAACGAGCTTGGGTGATAGTTGCACTTCTTATTATGTGTATGCATACGATGCCTAATTCTACATATGCACAAATGAA
CAATGTGAGTGCCCATAATGCTGTATTAATGGAAGAACATTCAGGGCGTGTTTTATATGGGAAACGTGAACATGAACCAC
AAAAAATTGCTAGTATTACAAAAATCATGACAGCGTTACTAGCTGCTGAATCGGGAAAAATGAAGGAAATGGTCTCTATT
AGTAACAAGGCAGTTCGTGTAGAAGGCTCAGCGATTTATTTAAAACCTGGACAAAAAGTGAAATTAGAGGACTTAGTTTA
TGGTTTAATGCTTCGATCTGGTAATGATGCAGCGCAAGCGATTGCTGAAAAAGTTGGTGGAAGTATAGAGGGATTTGTGT
ATTTAATGAATGAAAAAGCAAAAGAAATTGGAATGAAAGATACTCACTTTTCGAACCCACATGGTTTAGATGGTGATGGC
ACGCATTATTCTTCTGCTTATGACATGGCACTTTTAACAAAGTATGCAATGGGAAATGAAACATTTCGAAAGATTTTTGG
GACAAAAACATACCAATCGGAATCGTGGGATTACCCATGGAAAAATAAACATAAGCTTGTTACGTCATATTATGAATTTG
CAACTGGAGGGAAAACAGGATTTACAAAAAAAGCAGGGCGTACCCTTGTAACGACAGCATCAAAAAATGGTTTGGATTTC
ATTGTAGTAACGCTAAAAGCCTCGAGTGATTGGGATGATCATATGTATTTATTTGATCAAGGATTTAAACGATATAAGTT
AACAAAAGTGTTAGCACAAGGGGCATTTCCGGAATTGAAAGAAAAGAAGTATGCTCATCATGTTTATACAAAGAATGGTT
TTGCAGTGCCGTTAGCAAGTGATGAAAAAAATAAAGTTTTATTAAGAGTCGAATTGGATAAACGTGCAAATTTAAAAGAT
GGAAACAAAGTAGGGAAAATAGTTATTTATGTAGGAAATGAAAAAGTAGGGGAACGAAATTTATTTTATAGTAAGAGGAA
ATTAGTAGCTACAACAGGGGCGTACTGGAATAATGTGAGAGAAATTTTTTCTCATATGATAGGTGTGAACGGCGATGGTT
AA

Upstream 100 bases:

>100_bases
TGCGTCTTTTCTTGTGGTGGCAGAAAGAATATTTTATTCATATTTTAAAAGGCCAAGCATACACTTGTACAAAAAAGTGC
CAAAAGGACGTGGGGGAAGT

Downstream 100 bases:

>100_bases
TCTTGTATGGGTGGCAATGGCAGTTATTGGAATTGTATATGCGATGGTAAATGGAACGATGGAAGAAGTAAATAAAGCTG
TTTTTGCAGGGGCAAAAGAT

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKRAWVIVALLIMCMHTMPNSTYAQMNNVSAHNAVLMEEHSGRVLYGKREHEPQKIASITKIMTALLAAESGKMKEMVSI
SNKAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQAIAEKVGGSIEGFVYLMNEKAKEIGMKDTHFSNPHGLDGDG
THYSSAYDMALLTKYAMGNETFRKIFGTKTYQSESWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKNGLDF
IVVTLKASSDWDDHMYLFDQGFKRYKLTKVLAQGAFPELKEKKYAHHVYTKNGFAVPLASDEKNKVLLRVELDKRANLKD
GNKVGKIVIYVGNEKVGERNLFYSKRKLVATTGAYWNNVREIFSHMIGVNGDG

Sequences:

>Translated_373_residues
MKRAWVIVALLIMCMHTMPNSTYAQMNNVSAHNAVLMEEHSGRVLYGKREHEPQKIASITKIMTALLAAESGKMKEMVSI
SNKAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQAIAEKVGGSIEGFVYLMNEKAKEIGMKDTHFSNPHGLDGDG
THYSSAYDMALLTKYAMGNETFRKIFGTKTYQSESWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKNGLDF
IVVTLKASSDWDDHMYLFDQGFKRYKLTKVLAQGAFPELKEKKYAHHVYTKNGFAVPLASDEKNKVLLRVELDKRANLKD
GNKVGKIVIYVGNEKVGERNLFYSKRKLVATTGAYWNNVREIFSHMIGVNGDG
>Mature_373_residues
MKRAWVIVALLIMCMHTMPNSTYAQMNNVSAHNAVLMEEHSGRVLYGKREHEPQKIASITKIMTALLAAESGKMKEMVSI
SNKAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQAIAEKVGGSIEGFVYLMNEKAKEIGMKDTHFSNPHGLDGDG
THYSSAYDMALLTKYAMGNETFRKIFGTKTYQSESWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKNGLDF
IVVTLKASSDWDDHMYLFDQGFKRYKLTKVLAQGAFPELKEKKYAHHVYTKNGFAVPLASDEKNKVLLRVELDKRANLKD
GNKVGKIVIYVGNEKVGERNLFYSKRKLVATTGAYWNNVREIFSHMIGVNGDG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=355, Percent_Identity=31.5492957746479, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082022, Length=269, Percent_Identity=33.0855018587361, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786851, Length=228, Percent_Identity=35.5263157894737, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI87082054, Length=228, Percent_Identity=25.4385964912281, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 41788; Mature: 41788

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRAWVIVALLIMCMHTMPNSTYAQMNNVSAHNAVLMEEHSGRVLYGKREHEPQKIASIT
CCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHH
KIMTALLAAESGKMKEMVSISNKAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQA
HHHHHHHHHCCCCHHHHHHHCCCEEEEECCEEEECCCCEEEHHHHHHHHHHCCCCHHHHH
IAEKVGGSIEGFVYLMNEKAKEIGMKDTHFSNPHGLDGDGTHYSSAYDMALLTKYAMGNE
HHHHHCCCCEEEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCH
TFRKIFGTKTYQSESWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKNGLDF
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCCEEEEECCCCCCEE
IVVTLKASSDWDDHMYLFDQGFKRYKLTKVLAQGAFPELKEKKYAHHVYTKNGFAVPLAS
EEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHEEEECCCEEEEECC
DEKNKVLLRVELDKRANLKDGNKVGKIVIYVGNEKVGERNLFYSKRKLVATTGAYWNNVR
CCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHECCCEEEEECCHHHHHHH
EIFSHMIGVNGDG
HHHHHHCCCCCCC
>Mature Secondary Structure
MKRAWVIVALLIMCMHTMPNSTYAQMNNVSAHNAVLMEEHSGRVLYGKREHEPQKIASIT
CCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCEEEECCCCCCHHHHHHHH
KIMTALLAAESGKMKEMVSISNKAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQA
HHHHHHHHHCCCCHHHHHHHCCCEEEEECCEEEECCCCEEEHHHHHHHHHHCCCCHHHHH
IAEKVGGSIEGFVYLMNEKAKEIGMKDTHFSNPHGLDGDGTHYSSAYDMALLTKYAMGNE
HHHHHCCCCEEEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCH
TFRKIFGTKTYQSESWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKNGLDF
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCCEEEEECCCCCCEE
IVVTLKASSDWDDHMYLFDQGFKRYKLTKVLAQGAFPELKEKKYAHHVYTKNGFAVPLAS
EEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHEEEECCCEEEEECC
DEKNKVLLRVELDKRANLKDGNKVGKIVIYVGNEKVGERNLFYSKRKLVATTGAYWNNVR
CCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHECCCEEEEECCHHHHHHH
EIFSHMIGVNGDG
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]