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The map label for this gene is yiaE [C]

Identifier: 152974940

GI number: 152974940

Start: 1247440

End: 1248411

Strand: Direct

Name: yiaE [C]

Synonym: Bcer98_1137

Alternate gene names: 152974940

Gene position: 1247440-1248411 (Clockwise)

Preceding gene: 152974939

Following gene: 152974943

Centisome position: 30.52

GC content: 36.42

Gene sequence:

>972_bases
TTGGCAAAAATATTAGTAGCAGGTAAAGTTCCAGAAATCGGATTACAATTATTAAAAGAACATGATGTAGAAATGTATGA
TGGAGAAGAATTAATCTCTGCCCAGGAATTAACAAAGCGTGTCCAAGATAAAGATGCTTTATTAAGTTTGTTGTCTACGA
AAGTGACAAAAGAGGTCATTGATGCAGCGCCGAATTTAAAAATTGTAGCAAACTACGGTGCTGGATACGACAATATCGCT
TATGAATATGCAGCGGAAAAGGGGATTCTTGTTACGAACACGCCGAAAGTGTCTACAGAAGCGACAGCAGAATTAACATT
TGCACTTTTACTAGCAGCAGCAAGAAGAATTCCAGAAGGTGATACGTTATGCCGTACAACAGGATTTAATGGCTGGGCTC
CACTATTCTTTCTCGGAAGAGAAGTATATGGGAAAACAATTGGAATTATCGGTCTTGGAGAAATTGGAAAAGCGGTTGCG
AAACGCGCAAAAGCATTTGGCATGAATGTGCTTTATACAGGACCAAATCGAAAACAAGAAGCTGAGCGAGAATTAGATGC
TACATATGTATCGCTAGAACAATTATTACAAACAGCAGACTTTATTACAATTAACTGTTCGTATAACAAAAGTTTGCACC
ACATGATAGATGAAGAACAATTTAAAATGATGAAAAAAACGGCGTATCTTATTAATGCAGCTCGAGGTCCAATTATAAAT
GAGTTAGCGCTTGTTCATGCATTAGAAAGAAATGAGATTGAAGGAGCAGCTCTTGATGTATTTGAATTTGAACCGAAAAT
TACAGAGCAATTAAAAGGACTAAAAAACGTTGTTCTTACTCCTCATGTTGGAAATGCAACATTTGAAACAAGAGACGCTA
TGGCGGAAATGACAGTGCGAAATATTTTAGCTGTATTAAAAGGAGAAGAAGCACTAACACCTGTAAATTCAAAAATATTG
GTAACAAAATAA

Upstream 100 bases:

>100_bases
CGATGTTCTAAACGATAATCAAATTTCTTGGCTTTTTTCATATATATTTTTTACAATAAATGAAGAAGTGTTCATTTTTG
AAATAGAAGAGGTGCTTATT

Downstream 100 bases:

>100_bases
GAGAAGTCTTCCTCAATGAGGAAGACTTCTCTTATTTTGTTTTGTTAGCTTTTTTTTTATGAGACGGTCTCTGTCGAATA
AATTGGGATAACATATACAA

Product: glycerate dehydrogenase

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MAKILVAGKVPEIGLQLLKEHDVEMYDGEELISAQELTKRVQDKDALLSLLSTKVTKEVIDAAPNLKIVANYGAGYDNIA
YEYAAEKGILVTNTPKVSTEATAELTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVYGKTIGIIGLGEIGKAVA
KRAKAFGMNVLYTGPNRKQEAERELDATYVSLEQLLQTADFITINCSYNKSLHHMIDEEQFKMMKKTAYLINAARGPIIN
ELALVHALERNEIEGAALDVFEFEPKITEQLKGLKNVVLTPHVGNATFETRDAMAEMTVRNILAVLKGEEALTPVNSKIL
VTK

Sequences:

>Translated_323_residues
MAKILVAGKVPEIGLQLLKEHDVEMYDGEELISAQELTKRVQDKDALLSLLSTKVTKEVIDAAPNLKIVANYGAGYDNIA
YEYAAEKGILVTNTPKVSTEATAELTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVYGKTIGIIGLGEIGKAVA
KRAKAFGMNVLYTGPNRKQEAERELDATYVSLEQLLQTADFITINCSYNKSLHHMIDEEQFKMMKKTAYLINAARGPIIN
ELALVHALERNEIEGAALDVFEFEPKITEQLKGLKNVVLTPHVGNATFETRDAMAEMTVRNILAVLKGEEALTPVNSKIL
VTK
>Mature_322_residues
AKILVAGKVPEIGLQLLKEHDVEMYDGEELISAQELTKRVQDKDALLSLLSTKVTKEVIDAAPNLKIVANYGAGYDNIAY
EYAAEKGILVTNTPKVSTEATAELTFALLLAAARRIPEGDTLCRTTGFNGWAPLFFLGREVYGKTIGIIGLGEIGKAVAK
RAKAFGMNVLYTGPNRKQEAERELDATYVSLEQLLQTADFITINCSYNKSLHHMIDEEQFKMMKKTAYLINAARGPIINE
LALVHALERNEIEGAALDVFEFEPKITEQLKGLKNVVLTPHVGNATFETRDAMAEMTVRNILAVLKGEEALTPVNSKILV
TK

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=315, Percent_Identity=38.7301587301587, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI23308577, Length=322, Percent_Identity=33.2298136645963, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI4557497, Length=288, Percent_Identity=36.1111111111111, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI61743967, Length=288, Percent_Identity=36.1111111111111, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI145580575, Length=305, Percent_Identity=34.0983606557377, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI145580578, Length=306, Percent_Identity=33.9869281045752, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI4557499, Length=306, Percent_Identity=33.9869281045752, Blast_Score=152, Evalue=4e-37,
Organism=Escherichia coli, GI87082289, Length=268, Percent_Identity=37.3134328358209, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1789279, Length=280, Percent_Identity=31.4285714285714, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787645, Length=258, Percent_Identity=30.2325581395349, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788660, Length=257, Percent_Identity=29.5719844357977, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081824, Length=263, Percent_Identity=28.8973384030418, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=307, Percent_Identity=35.1791530944625, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25147481, Length=287, Percent_Identity=28.9198606271777, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324055, Length=261, Percent_Identity=36.0153256704981, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=316, Percent_Identity=30.379746835443, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6322116, Length=313, Percent_Identity=29.3929712460064, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324964, Length=251, Percent_Identity=27.4900398406374, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321253, Length=183, Percent_Identity=26.775956284153, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571528, Length=310, Percent_Identity=38.3870967741935, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28574286, Length=318, Percent_Identity=36.1635220125786, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI45552429, Length=318, Percent_Identity=35.5345911949685, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI45551003, Length=318, Percent_Identity=35.5345911949685, Blast_Score=167, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24585514, Length=318, Percent_Identity=35.5345911949685, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI28574282, Length=318, Percent_Identity=35.5345911949685, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI28574284, Length=318, Percent_Identity=35.5345911949685, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI19921140, Length=294, Percent_Identity=32.6530612244898, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24646446, Length=264, Percent_Identity=34.8484848484849, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24646448, Length=264, Percent_Identity=34.8484848484849, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24646452, Length=264, Percent_Identity=34.8484848484849, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24646450, Length=264, Percent_Identity=34.8484848484849, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI62472511, Length=264, Percent_Identity=34.8484848484849, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24585516, Length=317, Percent_Identity=28.7066246056782, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 35513; Mature: 35381

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKILVAGKVPEIGLQLLKEHDVEMYDGEELISAQELTKRVQDKDALLSLLSTKVTKEVI
CCEEEEECCCCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAAPNLKIVANYGAGYDNIAYEYAAEKGILVTNTPKVSTEATAELTFALLLAAARRIPEG
HCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
DTLCRTTGFNGWAPLFFLGREVYGKTIGIIGLGEIGKAVAKRAKAFGMNVLYTGPNRKQE
CCEEEECCCCCCHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCCEEEEECCCCHHH
AERELDATYVSLEQLLQTADFITINCSYNKSLHHMIDEEQFKMMKKTAYLINAARGPIIN
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHCHHHHHHHHHHHHHHHCCCCCHHH
ELALVHALERNEIEGAALDVFEFEPKITEQLKGLKNVVLTPHVGNATFETRDAMAEMTVR
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHH
NILAVLKGEEALTPVNSKILVTK
HHHHHHCCCHHCCCCCCEEEEEC
>Mature Secondary Structure 
AKILVAGKVPEIGLQLLKEHDVEMYDGEELISAQELTKRVQDKDALLSLLSTKVTKEVI
CEEEEECCCCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAAPNLKIVANYGAGYDNIAYEYAAEKGILVTNTPKVSTEATAELTFALLLAAARRIPEG
HCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
DTLCRTTGFNGWAPLFFLGREVYGKTIGIIGLGEIGKAVAKRAKAFGMNVLYTGPNRKQE
CCEEEECCCCCCHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCCEEEEECCCCHHH
AERELDATYVSLEQLLQTADFITINCSYNKSLHHMIDEEQFKMMKKTAYLINAARGPIIN
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHCHHHHHHHHHHHHHHHCCCCCHHH
ELALVHALERNEIEGAALDVFEFEPKITEQLKGLKNVVLTPHVGNATFETRDAMAEMTVR
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHH
NILAVLKGEEALTPVNSKILVTK
HHHHHHCCCHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]