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The map label for this gene is hisH

Identifier: 152974934

GI number: 152974934

Start: 1244086

End: 1244715

Strand: Direct

Name: hisH

Synonym: Bcer98_1131

Alternate gene names: 152974934

Gene position: 1244086-1244715 (Clockwise)

Preceding gene: 152974933

Following gene: 152974935

Centisome position: 30.44

GC content: 37.94

Gene sequence:

>630_bases
TTGATTGCAATTATAGACTATGGCATGGGAAATATTCGGAGTATAGAACAAGCATTAACATCTATTGGAGTGGAACATCT
TGTGACGGATCGTCAACGAGAAATTGTAAAAAGCGATGGTGTTATTTTACCGGGTGTAGGTGCGTTTCCGAAAGCAATGG
AAGCTTTAGAGGCAAAAAAACTAGTAACTGTATTACAAGAATGCGGAAAGACAGGAAAACCGCTTCTTGGCATTTGTTTA
GGAATGCAGTTACTGTTTGAAAAGAGTGAAGAAATGAAAAGTTCCAATGGTCTAGGTTTGTTACCAGGGGTAGTTCGTAA
GCTACAAGTTCCGTATAAAATTCCGCATATGGGATGGAATCGGTTAACAAAAACGAAAGAAATGCCAATTTGGAATAGAG
TAGCAGATGGTTCGTTTGTGTATTATGTTCATTCTTATTATGCTGAATGTCCAAATGATATGATTTGTGGTACGAGTGAA
TATGGAATTTCTGTTCCAGGGCTTGTTGCAAAAGGAAATATATTTGGAGCGCAATTTCATCCTGAAAAGAGTGGGGAAAT
CGGAATACAAATGCTTGCAAATTTCAAAGGAGTGGTGAAACAATGGAAATCCTCCCAGCTATCGATTTAA

Upstream 100 bases:

>100_bases
AATAGAAGCTTTATTTAAAGCATTTGGACGTGCGCTTCGAGAAGCTGTAGATAAAAATGAAAAGATTGTCGGAATCAATT
CTACGAAAGGAATGTTGTGA

Downstream 100 bases:

>100_bases
AAGAAGGACGATGTGTTCGGCTTTATCAAGGAGAGTTCAACAAAGAAACAGTTGTGAATGAACATCCAGTTGCACAAGCG
ATGATATTTGAAAAAATGGG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MIAIIDYGMGNIRSIEQALTSIGVEHLVTDRQREIVKSDGVILPGVGAFPKAMEALEAKKLVTVLQECGKTGKPLLGICL
GMQLLFEKSEEMKSSNGLGLLPGVVRKLQVPYKIPHMGWNRLTKTKEMPIWNRVADGSFVYYVHSYYAECPNDMICGTSE
YGISVPGLVAKGNIFGAQFHPEKSGEIGIQMLANFKGVVKQWKSSQLSI

Sequences:

>Translated_209_residues
MIAIIDYGMGNIRSIEQALTSIGVEHLVTDRQREIVKSDGVILPGVGAFPKAMEALEAKKLVTVLQECGKTGKPLLGICL
GMQLLFEKSEEMKSSNGLGLLPGVVRKLQVPYKIPHMGWNRLTKTKEMPIWNRVADGSFVYYVHSYYAECPNDMICGTSE
YGISVPGLVAKGNIFGAQFHPEKSGEIGIQMLANFKGVVKQWKSSQLSI
>Mature_209_residues
MIAIIDYGMGNIRSIEQALTSIGVEHLVTDRQREIVKSDGVILPGVGAFPKAMEALEAKKLVTVLQECGKTGKPLLGICL
GMQLLFEKSEEMKSSNGLGLLPGVVRKLQVPYKIPHMGWNRLTKTKEMPIWNRVADGSFVYYVHSYYAECPNDMICGTSE
YGISVPGLVAKGNIFGAQFHPEKSGEIGIQMLANFKGVVKQWKSSQLSI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=195, Percent_Identity=35.8974358974359, Blast_Score=127, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=30.7692307692308, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_BACCN (A7GMU8)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001374451.1
- ProteinModelPortal:   A7GMU8
- SMR:   A7GMU8
- STRING:   A7GMU8
- EnsemblBacteria:   EBBACT00000038979
- GeneID:   5344760
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_1131
- eggNOG:   COG0118
- GeneTree:   EBGT00050000000583
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- ProtClustDB:   PRK13141
- BioCyc:   BCER315749:BCER98_1131-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22946; Mature: 22946

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 180-180 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIIDYGMGNIRSIEQALTSIGVEHLVTDRQREIVKSDGVILPGVGAFPKAMEALEAKK
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHH
LVTVLQECGKTGKPLLGICLGMQLLFEKSEEMKSSNGLGLLPGVVRKLQVPYKIPHMGWN
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEECCCCCHH
RLTKTKEMPIWNRVADGSFVYYVHSYYAECPNDMICGTSEYGISVPGLVAKGNIFGAQFH
HHCCCCCCCCHHCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCEECCEEC
PEKSGEIGIQMLANFKGVVKQWKSSQLSI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIAIIDYGMGNIRSIEQALTSIGVEHLVTDRQREIVKSDGVILPGVGAFPKAMEALEAKK
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHH
LVTVLQECGKTGKPLLGICLGMQLLFEKSEEMKSSNGLGLLPGVVRKLQVPYKIPHMGWN
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEECCCCCHH
RLTKTKEMPIWNRVADGSFVYYVHSYYAECPNDMICGTSEYGISVPGLVAKGNIFGAQFH
HHCCCCCCCCHHCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCEECCEEC
PEKSGEIGIQMLANFKGVVKQWKSSQLSI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA