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The map label for this gene is ilvB [H]

Identifier: 152974924

GI number: 152974924

Start: 1232165

End: 1233865

Strand: Direct

Name: ilvB [H]

Synonym: Bcer98_1120

Alternate gene names: 152974924

Gene position: 1232165-1233865 (Clockwise)

Preceding gene: 152974921

Following gene: 152974925

Centisome position: 30.15

GC content: 41.27

Gene sequence:

>1701_bases
ATGGCTTCAAAAACAGAAGAAAAAATGGTAACTGGTGCAGAGCTATTGTTAGAAGCGCTAGAGAAGGAAGCGGTTGAGGT
GATTTTTGGATATCCAGGCGGTGCGGTTTTACCACTATATGATGCATTGTATGATTGTAAGATTCCTCATATTTTAACAA
GACATGAACAAGGTGCGATACATGCAGCAGAAGGATATGCCCGAATTACCGGGAATCCAGGTGTTGTCATTGCAACAAGT
GGACCAGGAGCAACAAACGTAATCACTGGGCTCGCCGATGCAATGATTGATTCATTGCCGCTCGTTGTTTTTACAGGGCA
AGTGGCTACAACTTTAATTGGAAGTGACGCATTCCAAGAAGCGGATATTATGGGACTTACCATGCCGGTAACAAAGCATA
ATTATCAAGTCCGAAAAGCTTCGGATTTACCCAGGATTATTAAAGAAGCATTTCATATTGCAAAAACAGGGCGACCGGGA
CCAGTTGTGATTGATCTCCCTAAAAATATGGTGATGGAGGAAGGGGAGCGATGCAGTGATGTGCAAATGGATTTACCCGG
GTATCAGCCGAATTATGAACCGAACGTATTACAAATTACCAAATTATCACAAGCGATTGCTACGGCTAAAAAACCGCTTA
TTTTAGCTGGTGCTGGTGTGCTACATGCAAAGGCTTCAAAAGAATTGACAAAATTTGCTCGCATGTACCGTCTACCAGTC
GTTCATACGCTTCTTGGATTAGGAGGATTCCCATCAGATGATGAATTGTTTTTAGGAATGGGAGGGATGCATGGGTCCTA
TACAGCGAACATGGCAATGTATGAATGTGATTTGCTTATCAATATTGGTGCAAGATTTGACGATCGGCTTACAGGGAATT
TAGCATATTTTGCGAAAAAGGCCACTGTTGCACATATTGATATCGATCCTGCAGAGATTGGTAAAAATGTCCCAACGGCT
ATCCCGATTGTTGCAAGTGCGAAGCGAGCTTTAGAAGCACTATTAGAACGTAAAGAGCGAAAAGAGAATCATGAAGAGTG
GCTTACGCTTTTAAAAAAGAGAAAAGAACAATATCCGTTTTTTTATAAACAAGATGCAGAAAAAATGAAACCGCAAGCAG
CAATTGATATGTTGTATGAAATTACAAAGGGGACAGCGATTATTACGACGGATGTTGGTCAACATCAAATGTGGGCTGCT
CAATATTACCCACTTAAAGAGCCGAATAAATGGGTAACATCAGGAGGACTTGGAACGATGGGATTTGGGCTTCCAGCAGC
AATTGGTGCACAAATTGCTAAGCCAACTGAATTAGTGGTTGCGATTGTCGGTGATGCTGGTTTTCAAATGACATTGCAAG
AATTAAGTGTTTTAAAAGAACATTCTTTACCCGTTAAAGTGTTAATTTTAAATAATGAGGCGTTAGGAATGGTAAGACAG
TGGCAAGATGAGTTTTATCAGCAAAGATATTCTCATTCGCTCCTTTCTTGTCAGCCGGATTTTGTGACATTAGCACAAGC
TTATGGCATAAAAGGCATAAGAATTCAAGATCCACTGCGAGCGAAAGAAGAATTACAAGCGGCAATGACATTAGATGAAC
CTGTCGTAATTGATTGCCGAGTGTTGCAGTCTGAAAAAGTAATGCCGATGGTTGCTCCAGGAAAAGGAGTCCATCAAATG
GAGGGGGTGAAAAGGAAATGA

Upstream 100 bases:

>100_bases
CAAAGAAGGGTGGTACCACGAAATCTTTCGTCCCTTCAGCACATAGCTGAAGTGTGGACGTAGGATTTTTTTATTTGAGA
AAAAAGGAGGGATACAATCG

Downstream 100 bases:

>100_bases
AAAGAATCGTGACTGCAACTGTACGAAATCAAAGTGGTGTATTAAACCGGATTACAGGAGTGATGACACGAAGGCACTTT
AATATTGAGAGTATTTCCGT

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MASKTEEKMVTGAELLLEALEKEAVEVIFGYPGGAVLPLYDALYDCKIPHILTRHEQGAIHAAEGYARITGNPGVVIATS
GPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIAKTGRPG
PVVIDLPKNMVMEEGERCSDVQMDLPGYQPNYEPNVLQITKLSQAIATAKKPLILAGAGVLHAKASKELTKFARMYRLPV
VHTLLGLGGFPSDDELFLGMGGMHGSYTANMAMYECDLLINIGARFDDRLTGNLAYFAKKATVAHIDIDPAEIGKNVPTA
IPIVASAKRALEALLERKERKENHEEWLTLLKKRKEQYPFFYKQDAEKMKPQAAIDMLYEITKGTAIITTDVGQHQMWAA
QYYPLKEPNKWVTSGGLGTMGFGLPAAIGAQIAKPTELVVAIVGDAGFQMTLQELSVLKEHSLPVKVLILNNEALGMVRQ
WQDEFYQQRYSHSLLSCQPDFVTLAQAYGIKGIRIQDPLRAKEELQAAMTLDEPVVIDCRVLQSEKVMPMVAPGKGVHQM
EGVKRK

Sequences:

>Translated_566_residues
MASKTEEKMVTGAELLLEALEKEAVEVIFGYPGGAVLPLYDALYDCKIPHILTRHEQGAIHAAEGYARITGNPGVVIATS
GPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIAKTGRPG
PVVIDLPKNMVMEEGERCSDVQMDLPGYQPNYEPNVLQITKLSQAIATAKKPLILAGAGVLHAKASKELTKFARMYRLPV
VHTLLGLGGFPSDDELFLGMGGMHGSYTANMAMYECDLLINIGARFDDRLTGNLAYFAKKATVAHIDIDPAEIGKNVPTA
IPIVASAKRALEALLERKERKENHEEWLTLLKKRKEQYPFFYKQDAEKMKPQAAIDMLYEITKGTAIITTDVGQHQMWAA
QYYPLKEPNKWVTSGGLGTMGFGLPAAIGAQIAKPTELVVAIVGDAGFQMTLQELSVLKEHSLPVKVLILNNEALGMVRQ
WQDEFYQQRYSHSLLSCQPDFVTLAQAYGIKGIRIQDPLRAKEELQAAMTLDEPVVIDCRVLQSEKVMPMVAPGKGVHQM
EGVKRK
>Mature_565_residues
ASKTEEKMVTGAELLLEALEKEAVEVIFGYPGGAVLPLYDALYDCKIPHILTRHEQGAIHAAEGYARITGNPGVVIATSG
PGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIAKTGRPGP
VVIDLPKNMVMEEGERCSDVQMDLPGYQPNYEPNVLQITKLSQAIATAKKPLILAGAGVLHAKASKELTKFARMYRLPVV
HTLLGLGGFPSDDELFLGMGGMHGSYTANMAMYECDLLINIGARFDDRLTGNLAYFAKKATVAHIDIDPAEIGKNVPTAI
PIVASAKRALEALLERKERKENHEEWLTLLKKRKEQYPFFYKQDAEKMKPQAAIDMLYEITKGTAIITTDVGQHQMWAAQ
YYPLKEPNKWVTSGGLGTMGFGLPAAIGAQIAKPTELVVAIVGDAGFQMTLQELSVLKEHSLPVKVLILNNEALGMVRQW
QDEFYQQRYSHSLLSCQPDFVTLAQAYGIKGIRIQDPLRAKEELQAAMTLDEPVVIDCRVLQSEKVMPMVAPGKGVHQME
GVKRK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=555, Percent_Identity=25.2252252252252, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI21361361, Length=515, Percent_Identity=24.4660194174757, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=46.643109540636, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1790104, Length=561, Percent_Identity=40.9982174688057, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1786717, Length=541, Percent_Identity=35.1201478743068, Blast_Score=312, Evalue=4e-86,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=28.4926470588235, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1788716, Length=499, Percent_Identity=25.250501002004, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=558, Percent_Identity=27.2401433691756, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=558, Percent_Identity=27.2401433691756, Blast_Score=166, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=580, Percent_Identity=25.3448275862069, Blast_Score=135, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=574, Percent_Identity=44.5993031358885, Blast_Score=485, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6320816, Length=485, Percent_Identity=24.3298969072165, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323163, Length=511, Percent_Identity=23.2876712328767, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922626, Length=564, Percent_Identity=25.177304964539, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62021; Mature: 61890

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKTEEKMVTGAELLLEALEKEAVEVIFGYPGGAVLPLYDALYDCKIPHILTRHEQGAI
CCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCCHHHHCCCCCCE
HAAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQE
EECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCHHCC
ADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIAKTGRPGPVVIDLPKNMVMEEGERCSD
CCEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHCCCCCCC
VQMDLPGYQPNYEPNVLQITKLSQAIATAKKPLILAGAGVLHAKASKELTKFARMYRLPV
CEEECCCCCCCCCCCEEEEHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
VHTLLGLGGFPSDDELFLGMGGMHGSYTANMAMYECDLLINIGARFDDRLTGNLAYFAKK
HHHHHHCCCCCCCCCEEEEECCCCCCEECCEEEEEEEEEEECCCCCCCCCCCCHHEEEEC
ATVAHIDIDPAEIGKNVPTAIPIVASAKRALEALLERKERKENHEEWLTLLKKRKEQYPF
CEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCE
FYKQDAEKMKPQAAIDMLYEITKGTAIITTDVGQHQMWAAQYYPLKEPNKWVTSGGLGTM
EEECCHHHHCHHHHHHHHHHHHCCCEEEEECCCCCCEEHHHCCCCCCCCCEEECCCCCCC
GFGLPAAIGAQIAKPTELVVAIVGDAGFQMTLQELSVLKEHSLPVKVLILNNEALGMVRQ
CCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
WQDEFYQQRYSHSLLSCQPDFVTLAQAYGIKGIRIQDPLRAKEELQAAMTLDEPVVIDCR
HHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCEEEEEE
VLQSEKVMPMVAPGKGVHQMEGVKRK
EECCCCCCEEECCCCCCHHHCCCCCC
>Mature Secondary Structure 
ASKTEEKMVTGAELLLEALEKEAVEVIFGYPGGAVLPLYDALYDCKIPHILTRHEQGAI
CCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCCHHHHCCCCCCE
HAAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQE
EECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCHHCC
ADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIAKTGRPGPVVIDLPKNMVMEEGERCSD
CCEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHCCCCCCC
VQMDLPGYQPNYEPNVLQITKLSQAIATAKKPLILAGAGVLHAKASKELTKFARMYRLPV
CEEECCCCCCCCCCCEEEEHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
VHTLLGLGGFPSDDELFLGMGGMHGSYTANMAMYECDLLINIGARFDDRLTGNLAYFAKK
HHHHHHCCCCCCCCCEEEEECCCCCCEECCEEEEEEEEEEECCCCCCCCCCCCHHEEEEC
ATVAHIDIDPAEIGKNVPTAIPIVASAKRALEALLERKERKENHEEWLTLLKKRKEQYPF
CEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCE
FYKQDAEKMKPQAAIDMLYEITKGTAIITTDVGQHQMWAAQYYPLKEPNKWVTSGGLGTM
EEECCHHHHCHHHHHHHHHHHHCCCEEEEECCCCCCEEHHHCCCCCCCCCEEECCCCCCC
GFGLPAAIGAQIAKPTELVVAIVGDAGFQMTLQELSVLKEHSLPVKVLILNNEALGMVRQ
CCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
WQDEFYQQRYSHSLLSCQPDFVTLAQAYGIKGIRIQDPLRAKEELQAAMTLDEPVVIDCR
HHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCEEEEEE
VLQSEKVMPMVAPGKGVHQMEGVKRK
EECCCCCCEEECCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]