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The map label for this gene is amaA [H]

Identifier: 152974898

GI number: 152974898

Start: 1204301

End: 1205485

Strand: Direct

Name: amaA [H]

Synonym: Bcer98_1094

Alternate gene names: 152974898

Gene position: 1204301-1205485 (Clockwise)

Preceding gene: 152974892

Following gene: 152974899

Centisome position: 29.47

GC content: 39.16

Gene sequence:

>1185_bases
ATGAATGATAAAATGATAAAATGGCAAAAGCTTATCTCAGAGAAAAATATAATAAAGTGGCGTCGCCATCTTCATCAGTT
TCCTGAACTTTCATTCCATGAAAAACAAACTTCTCAGTTTATCTATGATATATTGTGCTCCTTTTCCGCTTTTGAAGTAA
CAAGACCAACAAAATATAGTGTGATGGCAAAGAAAAAGGGTAAGAAACGCGGAAAAGTTGTCGCCATTCGAGCAGATATA
GATGCGTTACCAATTCAAGAAGAGAACGAAAAGCCGTATACATCATTCGTTTCAGATGTTATGCATGCATGTGGTCATGA
TGCACATACAGCTATTTTATTAGGAGCAGCAGAGGCGATTGCAAAAATGGAGGAAGAATGGGCTGGCGAGATTCGGTTAT
TCTTTCAACATGCAGAAGAGGTTTATCCTGGTGGTGCTCAAGAAATGGTAAAGGCTGGGGTTATGGAGGGAGTAGATTGT
ATTATAGGTCTGCATGTCATGTCTGGGTTAGAAACCGGTAAAATTGGAATCGTATACGGGCCTATGATGGCAGCGCCAGA
TGTATTTACAATTGAAGTGAAGGGAAGGGGGGGGCATGCGGCTAGACCTGAAGAAACAATTGATCCGATAGCAGTAGGAG
CGCAAATCATTACGAATTTACAACATATTGTTTCGAGAAATACAGGTGTTTTTGAACAAAGAGTTGTCTCTGTTACACAA
TTTCATGGCGGAACAGCGGATAATATTATTCCTAATACAGCATTTTTAATGGGAACGGTACGGTCTTTTCAGCAAGATGT
TAGAAAAGAAGCAGAAGAAAAAATTAACCAAATTGTAAAAGGTGTTACAGAAGCACATGGCGCTACATATGAATATACAT
ATCGCTATGGATATGATCCAGTGATTAACGATGTAGAGGTTACAAAAGTTGTCGAAGAAAGTGCTCGAGTGCTATTGGGA
AGTGACAGCATTATACAACTGGGTCCTTCCATGGGAGGAGAAGATTTTTCTGCTTATTTAAGAAAAGCTCCAGGCTGTTT
CTTTAAACTAGGGACTGGGAATGAAACAAAAAAGACGTGTTATCCACATCATCATCCGAAATTTGATGTAGATGAGTCGG
CACTTATGTATGGTGCGATGCTATTTTTAGAAGCGACAATGAAACTATTAGAAATCGAGTGTTGA

Upstream 100 bases:

>100_bases
TAATGAATTTGTCATGTAAAACGTAGAAATCCTAAAAGACTGCCTTTAAAAATAAATATATTGAAATAGAGGACGAACGA
TTAGAAGGAGGGTATTAGGG

Downstream 100 bases:

>100_bases
TATAAAGTGAACCTTTAATCAGTGGGGAAGTTCTTCACCAACCGAATTATTACCGCTTTAAAATAGCCGGGATAAAAAAA
GGCAACTGCGAGAATCGCAG

Product: amidohydrolase

Products: NA

Alternate protein names: L-aminoacylase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNDKMIKWQKLISEKNIIKWRRHLHQFPELSFHEKQTSQFIYDILCSFSAFEVTRPTKYSVMAKKKGKKRGKVVAIRADI
DALPIQEENEKPYTSFVSDVMHACGHDAHTAILLGAAEAIAKMEEEWAGEIRLFFQHAEEVYPGGAQEMVKAGVMEGVDC
IIGLHVMSGLETGKIGIVYGPMMAAPDVFTIEVKGRGGHAARPEETIDPIAVGAQIITNLQHIVSRNTGVFEQRVVSVTQ
FHGGTADNIIPNTAFLMGTVRSFQQDVRKEAEEKINQIVKGVTEAHGATYEYTYRYGYDPVINDVEVTKVVEESARVLLG
SDSIIQLGPSMGGEDFSAYLRKAPGCFFKLGTGNETKKTCYPHHHPKFDVDESALMYGAMLFLEATMKLLEIEC

Sequences:

>Translated_394_residues
MNDKMIKWQKLISEKNIIKWRRHLHQFPELSFHEKQTSQFIYDILCSFSAFEVTRPTKYSVMAKKKGKKRGKVVAIRADI
DALPIQEENEKPYTSFVSDVMHACGHDAHTAILLGAAEAIAKMEEEWAGEIRLFFQHAEEVYPGGAQEMVKAGVMEGVDC
IIGLHVMSGLETGKIGIVYGPMMAAPDVFTIEVKGRGGHAARPEETIDPIAVGAQIITNLQHIVSRNTGVFEQRVVSVTQ
FHGGTADNIIPNTAFLMGTVRSFQQDVRKEAEEKINQIVKGVTEAHGATYEYTYRYGYDPVINDVEVTKVVEESARVLLG
SDSIIQLGPSMGGEDFSAYLRKAPGCFFKLGTGNETKKTCYPHHHPKFDVDESALMYGAMLFLEATMKLLEIEC
>Mature_394_residues
MNDKMIKWQKLISEKNIIKWRRHLHQFPELSFHEKQTSQFIYDILCSFSAFEVTRPTKYSVMAKKKGKKRGKVVAIRADI
DALPIQEENEKPYTSFVSDVMHACGHDAHTAILLGAAEAIAKMEEEWAGEIRLFFQHAEEVYPGGAQEMVKAGVMEGVDC
IIGLHVMSGLETGKIGIVYGPMMAAPDVFTIEVKGRGGHAARPEETIDPIAVGAQIITNLQHIVSRNTGVFEQRVVSVTQ
FHGGTADNIIPNTAFLMGTVRSFQQDVRKEAEEKINQIVKGVTEAHGATYEYTYRYGYDPVINDVEVTKVVEESARVLLG
SDSIIQLGPSMGGEDFSAYLRKAPGCFFKLGTGNETKKTCYPHHHPKFDVDESALMYGAMLFLEATMKLLEIEC

Specific function: Hydrolyzes most efficiently N-acetyl derivatives of aromatic amino acids but is also active on other amino acids. L- stereospecific [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=330, Percent_Identity=28.4848484848485, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.14 [H]

Molecular weight: Translated: 43896; Mature: 43896

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKMIKWQKLISEKNIIKWRRHLHQFPELSFHEKQTSQFIYDILCSFSAFEVTRPTKYS
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCHHH
VMAKKKGKKRGKVVAIRADIDALPIQEENEKPYTSFVSDVMHACGHDAHTAILLGAAEAI
HHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AKMEEEWAGEIRLFFQHAEEVYPGGAQEMVKAGVMEGVDCIIGLHVMSGLETGKIGIVYG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
PMMAAPDVFTIEVKGRGGHAARPEETIDPIAVGAQIITNLQHIVSRNTGVFEQRVVSVTQ
CCCCCCCEEEEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FHGGTADNIIPNTAFLMGTVRSFQQDVRKEAEEKINQIVKGVTEAHGATYEYTYRYGYDP
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
VINDVEVTKVVEESARVLLGSDSIIQLGPSMGGEDFSAYLRKAPGCFFKLGTGNETKKTC
CCCCHHHHHHHHHHHHEEECCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
YPHHHPKFDVDESALMYGAMLFLEATMKLLEIEC
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDKMIKWQKLISEKNIIKWRRHLHQFPELSFHEKQTSQFIYDILCSFSAFEVTRPTKYS
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCHHH
VMAKKKGKKRGKVVAIRADIDALPIQEENEKPYTSFVSDVMHACGHDAHTAILLGAAEAI
HHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AKMEEEWAGEIRLFFQHAEEVYPGGAQEMVKAGVMEGVDCIIGLHVMSGLETGKIGIVYG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
PMMAAPDVFTIEVKGRGGHAARPEETIDPIAVGAQIITNLQHIVSRNTGVFEQRVVSVTQ
CCCCCCCEEEEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FHGGTADNIIPNTAFLMGTVRSFQQDVRKEAEEKINQIVKGVTEAHGATYEYTYRYGYDP
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
VINDVEVTKVVEESARVLLGSDSIIQLGPSMGGEDFSAYLRKAPGCFFKLGTGNETKKTC
CCCCHHHHHHHHHHHHEEECCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
YPHHHPKFDVDESALMYGAMLFLEATMKLLEIEC
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8285691; 9023955 [H]