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The map label for this gene is fabI [H]

Identifier: 152974732

GI number: 152974732

Start: 1039438

End: 1040214

Strand: Direct

Name: fabI [H]

Synonym: Bcer98_0920

Alternate gene names: 152974732

Gene position: 1039438-1040214 (Clockwise)

Preceding gene: 152974728

Following gene: 152974736

Centisome position: 25.43

GC content: 40.54

Gene sequence:

>777_bases
ATGAATCTTCTCAACTTACAAGGGAAAAATATTATTGTAATGGGTGTTGCAAACCAACGAAGCATTGCGTGGGGCATTGC
TCGTTCATTGCATAACGCTGGAGCAAACTTAATCTTTACATACGCTGGTGAGCGTTTAGAGAAAAACGTGCGTGACTTAG
CGGAATCATTAGACGGTCAAAAGTCACTTGTTTTACCATGTGATATAACAAATGATGAAGATATCGCACGCTGCTTTGCT
ACAATTAAAGAAGAAGTTGGTGTCGTGCACGGATTAGCACACTGCATCGCATTTGCGAACCGCGATGATTTAAAAGGAGA
ATTTGTTGACACATCTCGCGATGGATTCTTACTTGCACAAAATATTAGTTCATTCTCTTTAACAGCAGTTGCCAAAGCTG
TGAAACCACTTATGACAGAAGGTGGAAGTATTGTCACATTAACATACCTTGGCGGAGAACGCGTTGTTGCTAACTATAAC
GTAATGGGGGTTGCAAAAGCTTCATTAGATGCAAGTGTAAGATACTTAGCAAATGACTTAGGTCAACATAATATTCGTGT
AAACTCAGTATCAGCAGGCCCAATTCGTACACTATCTGCAAAAGGTGTCGGAGACTTTAACAGCATTTTAAAACAAATTG
AAGAGCGCGCGCCACTTCGCCGTAATGTCACACAAGAAGAAGTTGGAAACACAGCGCTATTCTTATTTAGTGACTTAGCA
AGCGGTGTAACGGGTGAGAATATTCACGTGGATTCTGGGTATCATATTTTAGGATAA

Upstream 100 bases:

>100_bases
TAATTTTTATTTTGTCTTCTTGAGATTTTGTGATATGATATAATTATATTACCAGGTACTAATAACAGATTGTATTAAAT
AGATTTAGGGGGATAAATAA

Downstream 100 bases:

>100_bases
TAAGATAACGAATAATACGATAAAAAGGCACACATTTTTGTGCCTTTTTATTTTTTACTTTTTAGCAATTCCTCTAATTT
TGTTAAGGAGTGAAGAGCTT

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: Cold shock-induced protein 15; CSI15; NADH-dependent enoyl-ACP reductase; Vegetative protein 241; VEG241 [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNLLNLQGKNIIVMGVANQRSIAWGIARSLHNAGANLIFTYAGERLEKNVRDLAESLDGQKSLVLPCDITNDEDIARCFA
TIKEEVGVVHGLAHCIAFANRDDLKGEFVDTSRDGFLLAQNISSFSLTAVAKAVKPLMTEGGSIVTLTYLGGERVVANYN
VMGVAKASLDASVRYLANDLGQHNIRVNSVSAGPIRTLSAKGVGDFNSILKQIEERAPLRRNVTQEEVGNTALFLFSDLA
SGVTGENIHVDSGYHILG

Sequences:

>Translated_258_residues
MNLLNLQGKNIIVMGVANQRSIAWGIARSLHNAGANLIFTYAGERLEKNVRDLAESLDGQKSLVLPCDITNDEDIARCFA
TIKEEVGVVHGLAHCIAFANRDDLKGEFVDTSRDGFLLAQNISSFSLTAVAKAVKPLMTEGGSIVTLTYLGGERVVANYN
VMGVAKASLDASVRYLANDLGQHNIRVNSVSAGPIRTLSAKGVGDFNSILKQIEERAPLRRNVTQEEVGNTALFLFSDLA
SGVTGENIHVDSGYHILG
>Mature_258_residues
MNLLNLQGKNIIVMGVANQRSIAWGIARSLHNAGANLIFTYAGERLEKNVRDLAESLDGQKSLVLPCDITNDEDIARCFA
TIKEEVGVVHGLAHCIAFANRDDLKGEFVDTSRDGFLLAQNISSFSLTAVAKAVKPLMTEGGSIVTLTYLGGERVVANYN
VMGVAKASLDASVRYLANDLGQHNIRVNSVSAGPIRTLSAKGVGDFNSILKQIEERAPLRRNVTQEEVGNTALFLFSDLA
SGVTGENIHVDSGYHILG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=254, Percent_Identity=26.3779527559055, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI10190704, Length=115, Percent_Identity=39.1304347826087, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787545, Length=252, Percent_Identity=47.6190476190476, Blast_Score=244, Evalue=3e-66,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=28.8461538461538, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787905, Length=256, Percent_Identity=29.6875, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790717, Length=255, Percent_Identity=23.921568627451, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789378, Length=239, Percent_Identity=25.9414225941423, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787335, Length=264, Percent_Identity=29.1666666666667, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=256, Percent_Identity=30.46875, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI23397609, Length=265, Percent_Identity=26.7924528301887, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27713; Mature: 27713

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLNLQGKNIIVMGVANQRSIAWGIARSLHNAGANLIFTYAGERLEKNVRDLAESLDGQ
CCCEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCC
KSLVLPCDITNDEDIARCFATIKEEVGVVHGLAHCIAFANRDDLKGEFVDTSRDGFLLAQ
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEC
NISSFSLTAVAKAVKPLMTEGGSIVTLTYLGGERVVANYNVMGVAKASLDASVRYLANDL
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEECCEEEEEHHHHHHHHHHHHHHC
GQHNIRVNSVSAGPIRTLSAKGVGDFNSILKQIEERAPLRRNVTQEEVGNTALFLFSDLA
CCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCHHHCCCHHHHCCCCEEEHHHHH
SGVTGENIHVDSGYHILG
CCCCCCEEEECCCCEECC
>Mature Secondary Structure
MNLLNLQGKNIIVMGVANQRSIAWGIARSLHNAGANLIFTYAGERLEKNVRDLAESLDGQ
CCCEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCC
KSLVLPCDITNDEDIARCFATIKEEVGVVHGLAHCIAFANRDDLKGEFVDTSRDGFLLAQ
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEC
NISSFSLTAVAKAVKPLMTEGGSIVTLTYLGGERVVANYNVMGVAKASLDASVRYLANDL
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEECCEEEEEHHHHHHHHHHHHHHC
GQHNIRVNSVSAGPIRTLSAKGVGDFNSILKQIEERAPLRRNVTQEEVGNTALFLFSDLA
CCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCHHHCCCHHHHCCCCEEEHHHHH
SGVTGENIHVDSGYHILG
CCCCCCEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 8755892; 9298659 [H]