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The map label for this gene is lplA [H]

Identifier: 152974652

GI number: 152974652

Start: 953146

End: 954135

Strand: Direct

Name: lplA [H]

Synonym: Bcer98_0839

Alternate gene names: 152974652

Gene position: 953146-954135 (Clockwise)

Preceding gene: 152974651

Following gene: 152974653

Centisome position: 23.32

GC content: 34.75

Gene sequence:

>990_bases
ATGTTATTTATTGATAATAAAGGGATTACAGATCCTACGATTAATTTAGCAATTGAAGAATATTGCGTGAAAAATTTAGA
TATTAATGAAACATATTTGCTGTTTTACATTAATGAACCTTCCATCATTATTGGGAAAAACCAAAATACAGTCGAAGAAA
TTAATGCAGATTATGTGAAAGAGAAAGGGATCCATGTTGTACGCCGTTTATCAGGTGGCGGGGCTGTGTACCATGATTTA
GGAAACTTAAACTTTAGTTTTATTACAAAAGATGACGGGGATAGTTTTCATAACTTTAAAAAATTCACAGAGCCCGTTAC
GAAAGCACTTGGTAAATTAGGTGTGAATGCAGAACTTAGTGGACGTAACGATATTTTAGCTGAGGGACGAAAAATTTCAG
GTAACGCTCAATTTTCAACGAGAGGTCGTATGTTCAGTCACGGTACATTATTATTTGATTCTGAAATCGATCACGTTGTA
TCTGCATTAAAAGTGAAAATGGACAAAATTCAATCAAAAGGAATTAAATCGATTCGCAGCCGTGTAGCTAATATTACGGA
ATTTTTAAATGAAAAGATGACAACAGAAGAATTTAAACAGCTTCTTCTTGAGACAATCTTTGAAGGAGAAAAGGAAATTC
CAACATATGAGTTAACAGAAGAAGATTGGAAAGAAATTTATAAAATTTCTGAAGAGCGCTACCGCAATTGGGACTGGAAC
TACGGAAAGTCACCGAAGTTCAATTTACAACATTCACACCGTTTCCCAGTTGGACAGGTTGATGTTCGATTAGAAGTGAA
AAAAGGAACAGTAACAGAGTGTAAAATATATGGTGACTTCTTTGGTGCACTCGATGTTCATGATATTGAAGAGCGTTTAA
CAGGTGTTCAGTTTGATAAGGAAGCCTTTGCTGCAGCGTTAGAAGACGTAGATGTAAAACGCTACTTTGGGAATATAACG
AAAGAAGATTTCTTAGCATTGTTCTTTTAA

Upstream 100 bases:

>100_bases
AGGAAATCCAGCTGATTTAGTAGAAGAAGCAAAACAAATTTTTAGTGGCTCTATTGCATTAGCGCATAGTGGCTATACTT
GGAATTCATGAGGTGAGAAC

Downstream 100 bases:

>100_bases
TAGTGGACTGCTCTCCTAAATTGGGAGAGCAGTTTTCATGTCGTTATTTGTCGTACCGTCGATATCCTGTGTCGAATGGC
CGATATATTTTAAATTGCGC

Product: lipoyltransferase and lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MLFIDNKGITDPTINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVKEKGIHVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFHNFKKFTEPVTKALGKLGVNAELSGRNDILAEGRKISGNAQFSTRGRMFSHGTLLFDSEIDHVV
SALKVKMDKIQSKGIKSIRSRVANITEFLNEKMTTEEFKQLLLETIFEGEKEIPTYELTEEDWKEIYKISEERYRNWDWN
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGALDVHDIEERLTGVQFDKEAFAAALEDVDVKRYFGNIT
KEDFLALFF

Sequences:

>Translated_329_residues
MLFIDNKGITDPTINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVKEKGIHVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFHNFKKFTEPVTKALGKLGVNAELSGRNDILAEGRKISGNAQFSTRGRMFSHGTLLFDSEIDHVV
SALKVKMDKIQSKGIKSIRSRVANITEFLNEKMTTEEFKQLLLETIFEGEKEIPTYELTEEDWKEIYKISEERYRNWDWN
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGALDVHDIEERLTGVQFDKEAFAAALEDVDVKRYFGNIT
KEDFLALFF
>Mature_329_residues
MLFIDNKGITDPTINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVKEKGIHVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFHNFKKFTEPVTKALGKLGVNAELSGRNDILAEGRKISGNAQFSTRGRMFSHGTLLFDSEIDHVV
SALKVKMDKIQSKGIKSIRSRVANITEFLNEKMTTEEFKQLLLETIFEGEKEIPTYELTEEDWKEIYKISEERYRNWDWN
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGALDVHDIEERLTGVQFDKEAFAAALEDVDVKRYFGNIT
KEDFLALFF

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=297, Percent_Identity=30.976430976431, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI21729884, Length=297, Percent_Identity=30.976430976431, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI21729882, Length=297, Percent_Identity=30.976430976431, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI21729880, Length=297, Percent_Identity=30.976430976431, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI21729878, Length=297, Percent_Identity=30.976430976431, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI1790846, Length=299, Percent_Identity=34.1137123745819, Blast_Score=174, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI71981909, Length=244, Percent_Identity=36.8852459016393, Blast_Score=121, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322415, Length=283, Percent_Identity=30.0353356890459, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI28573464, Length=283, Percent_Identity=31.4487632508834, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 37743; Mature: 37743

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFIDNKGITDPTINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVK
CEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCHHHHHCHHHHH
EKGIHVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFHNFKKFTEPVTKALGKLGVNAELS
HCCHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDILAEGRKISGNAQFSTRGRMFSHGTLLFDSEIDHVVSALKVKMDKIQSKGIKSIRS
CCCCHHHCCCCCCCCCEECCCCCEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVANITEFLNEKMTTEEFKQLLLETIFEGEKEIPTYELTEEDWKEIYKISEERYRNWDWN
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCC
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGALDVHDIEERLTGVQFDK
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEHHHCCCCHHHHHHHHCCCCCCH
EAFAAALEDVDVKRYFGNITKEDFLALFF
HHHHHHHHHHHHHHHHCCCCHHHHHEECC
>Mature Secondary Structure
MLFIDNKGITDPTINLAIEEYCVKNLDINETYLLFYINEPSIIIGKNQNTVEEINADYVK
CEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCHHHHHCHHHHH
EKGIHVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFHNFKKFTEPVTKALGKLGVNAELS
HCCHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDILAEGRKISGNAQFSTRGRMFSHGTLLFDSEIDHVVSALKVKMDKIQSKGIKSIRS
CCCCHHHCCCCCCCCCEECCCCCEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVANITEFLNEKMTTEEFKQLLLETIFEGEKEIPTYELTEEDWKEIYKISEERYRNWDWN
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCC
YGKSPKFNLQHSHRFPVGQVDVRLEVKKGTVTECKIYGDFFGALDVHDIEERLTGVQFDK
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEHHHCCCCHHHHHHHHCCCCCCH
EAFAAALEDVDVKRYFGNITKEDFLALFF
HHHHHHHHHHHHHHHHCCCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]