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The map label for this gene is pbpF [H]

Identifier: 152974637

GI number: 152974637

Start: 936917

End: 939034

Strand: Direct

Name: pbpF [H]

Synonym: Bcer98_0823

Alternate gene names: 152974637

Gene position: 936917-939034 (Clockwise)

Preceding gene: 152974635

Following gene: 152974638

Centisome position: 22.92

GC content: 38.48

Gene sequence:

>2118_bases
ATGAAGAAAATAAAGTGGACTGTAATAAGCGGCATAGCAGTGTTTATAGTTGCAATCGTAATCTATAAACTTATTGTGTT
AGCTGGTGGCTATATGATGGATGAAAAGCAGTTGGTTTTCCACTCTTCATCACGTATCGTTGATCAGAAAGGAAAAGAAA
TTACAAAATTATATATAGAAAATCGAGAACTTGTGCCAATTGAGCAAATCCCAAAGCATGTGCAGCAAGCATTTCTTGCA
GTAGAAGATGCACGGTTTTATGAACATCACGGCATTGATTATCCTTCTATTTTTAGGGCGGTGTATCGGGATCTTTTAGC
AAGAGAGAAAGTGCAAGGAGGTAGTACGATTACGCAGCAACTCGTCAAAAATGTATTTTTAACGCATGAAAAAACATTTG
TACGTAAGTTGAAAGAAGTTGCGATAGCACTGCAAATCGAGCAAAAATATACAAAACAGCAAATTCTTGAAATGTATATG
AACCATATTTACTTTGGTCATGGTGCATACGGAATTCAATCTGCAGCCAAAATGTACTTTAATAAAAATGTAGAGGACTT
AACTGTCGAAGAGGGAGCGATGTTAGCTGGGCTTTTGAAAGCACCAAGCGGATATTCTCCGTTTTTACATCCGAAAAAGA
GTAAGGAGCGCCGTGATTTAGTTTTGTCTCTCATGCATAATCAAGGGTATATAACAGCAGAGGAAAGCGTTCGTTATCAA
GGGAAAACCATTGCGCTATATAAAAATCTAGATAAACAGGAACTCGCCTATATGCCTTATGTCGATATGGTCGTGGATGA
AGCAGCACGTCTATATGGCTTGTCACATCAAGAAGTCCTCCGCGGCGGTTATACGTTTGTTGTTTCCATGGATGAAAATA
TGCAAAAGGTTGCTTATGATCAGTTTCAAGATGAAAAAAATTTCCCAGGGGGAGACAAAAATGTGCAAGGCGCTTTTTTA
TTAATGGATAATCAGACGGGAGGAATTAAGGCCGCGATTGGCGGAAGAGAATATGTTCCAAGAGGTTTTAATCGAGTATT
TGCAAAGCGTCAGCCAGGGTCTGTTTTAAAGCCCCTTCTTGTATATGCGCCAGCACTGGAAACAAAAAAGTACAATCCGT
ATTCTTTATTAACAAATGAAAGAACTGCGTTTAAAGGATATGTACCGCGAAATTATAATCATCAATATTCAAAGGAAGTA
ACGATGTATGATGCGCTCTTGCAGTCAGCGAATGTTCCTGCAGTTTCTTTACTGCATGAACTTGGAGTAGAAGAAGGGAA
GCAATATTTGGAGAAGGGAAATATTCACATTGCTGATTCAGGTTTAAGCGCCGCTCTTGGTGGATTAAAAGAAGGGGTTT
CTCCGTTTGAAATTGTGAAGATGTATCGTGCGTTTCTAGCAAATGGAAAAATTATTGAGCCACATGTCATTGAAAAAGTA
TTTGATCGACAGGGGAAAATGATTGGAGAGTCCCAAAAAGAAGAAACGAAAATTTTCTCAAAGCAAACTGCGTGGTATAT
GACAAAAATGTTAGAAGGGGTTGTAGAGGAAGGAACAGCAAGGGCAGGAGTGTATAAAGGGGCACTAGCTGGAAAGACAG
GGACGACTTCGCTTTCCAACCAACGTGAGGGTGTAAGGGATATTTGGTTTGTTGGATATACGCCAAGTTTAGTAGGTGCG
GTATGGATGGGATATGATCGTACAGATAAAAAACATACGTTATATGGTGGAAGTGAGTCTGCTACCGAGCTATTTAAAAA
GATTTTAATGAAAGCTCATGTGCAGGACAAAGAAAAGTTTAAGCGACCAAAAGGAGTTGCGACGATTAGTAAGCCCATTC
ATTTAAGTAAAATTGAACATTTAGAAGCAAAAATATCTTTTCATCCGTTTGGCTTATTCATTGCAAAATTAAGCTGGAAA
CCTCTTCCTGATAAAAGGGTGATGTACAGAATTTATCAAATAGAAAATGGAGTTCATACGCATATTGGAACGGTTAAAGG
TACGGGCGAATATGATGTGAAATTTATTAATATATTTGCAAAACCGAGCTTTTACGTTGTTCCATATAACTCGCAAACAA
ATCGGGAAGGAGAAAAGACAAAAGTAGCTAAACCGTAG

Upstream 100 bases:

>100_bases
TATTATAGCAACTCTTACTAAAAACTTCGAAGAATATATACATAAAGGAGAGCCTTTTTCAAATAATAAATGTACCATCA
TTTGGAAAGAAGGACGAAGT

Downstream 100 bases:

>100_bases
TTTTTGCTTGTGATATAATAAGAATGTTTCGAAAATATCAAGCGTTTTTACGAACATTTGTGTCAATTTCATGAACAACA
TACATAATGTTATGCATTTT

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MKKIKWTVISGIAVFIVAIVIYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYIENRELVPIEQIPKHVQQAFLA
VEDARFYEHHGIDYPSIFRAVYRDLLAREKVQGGSTITQQLVKNVFLTHEKTFVRKLKEVAIALQIEQKYTKQQILEMYM
NHIYFGHGAYGIQSAAKMYFNKNVEDLTVEEGAMLAGLLKAPSGYSPFLHPKKSKERRDLVLSLMHNQGYITAEESVRYQ
GKTIALYKNLDKQELAYMPYVDMVVDEAARLYGLSHQEVLRGGYTFVVSMDENMQKVAYDQFQDEKNFPGGDKNVQGAFL
LMDNQTGGIKAAIGGREYVPRGFNRVFAKRQPGSVLKPLLVYAPALETKKYNPYSLLTNERTAFKGYVPRNYNHQYSKEV
TMYDALLQSANVPAVSLLHELGVEEGKQYLEKGNIHIADSGLSAALGGLKEGVSPFEIVKMYRAFLANGKIIEPHVIEKV
FDRQGKMIGESQKEETKIFSKQTAWYMTKMLEGVVEEGTARAGVYKGALAGKTGTTSLSNQREGVRDIWFVGYTPSLVGA
VWMGYDRTDKKHTLYGGSESATELFKKILMKAHVQDKEKFKRPKGVATISKPIHLSKIEHLEAKISFHPFGLFIAKLSWK
PLPDKRVMYRIYQIENGVHTHIGTVKGTGEYDVKFINIFAKPSFYVVPYNSQTNREGEKTKVAKP

Sequences:

>Translated_705_residues
MKKIKWTVISGIAVFIVAIVIYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYIENRELVPIEQIPKHVQQAFLA
VEDARFYEHHGIDYPSIFRAVYRDLLAREKVQGGSTITQQLVKNVFLTHEKTFVRKLKEVAIALQIEQKYTKQQILEMYM
NHIYFGHGAYGIQSAAKMYFNKNVEDLTVEEGAMLAGLLKAPSGYSPFLHPKKSKERRDLVLSLMHNQGYITAEESVRYQ
GKTIALYKNLDKQELAYMPYVDMVVDEAARLYGLSHQEVLRGGYTFVVSMDENMQKVAYDQFQDEKNFPGGDKNVQGAFL
LMDNQTGGIKAAIGGREYVPRGFNRVFAKRQPGSVLKPLLVYAPALETKKYNPYSLLTNERTAFKGYVPRNYNHQYSKEV
TMYDALLQSANVPAVSLLHELGVEEGKQYLEKGNIHIADSGLSAALGGLKEGVSPFEIVKMYRAFLANGKIIEPHVIEKV
FDRQGKMIGESQKEETKIFSKQTAWYMTKMLEGVVEEGTARAGVYKGALAGKTGTTSLSNQREGVRDIWFVGYTPSLVGA
VWMGYDRTDKKHTLYGGSESATELFKKILMKAHVQDKEKFKRPKGVATISKPIHLSKIEHLEAKISFHPFGLFIAKLSWK
PLPDKRVMYRIYQIENGVHTHIGTVKGTGEYDVKFINIFAKPSFYVVPYNSQTNREGEKTKVAKP
>Mature_705_residues
MKKIKWTVISGIAVFIVAIVIYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYIENRELVPIEQIPKHVQQAFLA
VEDARFYEHHGIDYPSIFRAVYRDLLAREKVQGGSTITQQLVKNVFLTHEKTFVRKLKEVAIALQIEQKYTKQQILEMYM
NHIYFGHGAYGIQSAAKMYFNKNVEDLTVEEGAMLAGLLKAPSGYSPFLHPKKSKERRDLVLSLMHNQGYITAEESVRYQ
GKTIALYKNLDKQELAYMPYVDMVVDEAARLYGLSHQEVLRGGYTFVVSMDENMQKVAYDQFQDEKNFPGGDKNVQGAFL
LMDNQTGGIKAAIGGREYVPRGFNRVFAKRQPGSVLKPLLVYAPALETKKYNPYSLLTNERTAFKGYVPRNYNHQYSKEV
TMYDALLQSANVPAVSLLHELGVEEGKQYLEKGNIHIADSGLSAALGGLKEGVSPFEIVKMYRAFLANGKIIEPHVIEKV
FDRQGKMIGESQKEETKIFSKQTAWYMTKMLEGVVEEGTARAGVYKGALAGKTGTTSLSNQREGVRDIWFVGYTPSLVGA
VWMGYDRTDKKHTLYGGSESATELFKKILMKAHVQDKEKFKRPKGVATISKPIHLSKIEHLEAKISFHPFGLFIAKLSWK
PLPDKRVMYRIYQIENGVHTHIGTVKGTGEYDVKFINIFAKPSFYVVPYNSQTNREGEKTKVAKP

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=564, Percent_Identity=30.3191489361702, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87082258, Length=249, Percent_Identity=43.7751004016064, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1788867, Length=533, Percent_Identity=26.6416510318949, Blast_Score=153, Evalue=4e-38,
Organism=Escherichia coli, GI1789601, Length=239, Percent_Identity=30.5439330543933, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 79853; Mature: 79853

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIKWTVISGIAVFIVAIVIYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYIE
CCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCCHHHHHCCCCEEHHEEC
NRELVPIEQIPKHVQQAFLAVEDARFYEHHGIDYPSIFRAVYRDLLAREKVQGGSTITQQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LVKNVFLTHEKTFVRKLKEVAIALQIEQKYTKQQILEMYMNHIYFGHGAYGIQSAAKMYF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
NKNVEDLTVEEGAMLAGLLKAPSGYSPFLHPKKSKERRDLVLSLMHNQGYITAEESVRYQ
CCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCEEC
GKTIALYKNLDKQELAYMPYVDMVVDEAARLYGLSHQEVLRGGYTFVVSMDENMQKVAYD
CCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHCCCEEEEEECCCCHHHHHHH
QFQDEKNFPGGDKNVQGAFLLMDNQTGGIKAAIGGREYVPRGFNRVFAKRQPGSVLKPLL
HHHCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHH
VYAPALETKKYNPYSLLTNERTAFKGYVPRNYNHQYSKEVTMYDALLQSANVPAVSLLHE
HHCCCCCCCCCCCCEEECCCCHHEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
LGVEEGKQYLEKGNIHIADSGLSAALGGLKEGVSPFEIVKMYRAFLANGKIIEPHVIEKV
HCHHHHHHHHHHCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECHHHHHHH
FDRQGKMIGESQKEETKIFSKQTAWYMTKMLEGVVEEGTARAGVYKGALAGKTGTTSLSN
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHCCCCCCCCCCCCCH
QREGVRDIWFVGYTPSLVGAVWMGYDRTDKKHTLYGGSESATELFKKILMKAHVQDKEKF
HCCCCCEEEEECCCHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHH
KRPKGVATISKPIHLSKIEHLEAKISFHPFGLFIAKLSWKPLPDKRVMYRIYQIENGVHT
CCCCCCEECCCCCHHHHHHHHHHEEEECHHEEEEEEECCCCCCCHHHHHHHHHHCCCCEE
HIGTVKGTGEYDVKFINIFAKPSFYVVPYNSQTNREGEKTKVAKP
ECCCEECCCCCCEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKIKWTVISGIAVFIVAIVIYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYIE
CCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCCHHHHHCCCCEEHHEEC
NRELVPIEQIPKHVQQAFLAVEDARFYEHHGIDYPSIFRAVYRDLLAREKVQGGSTITQQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LVKNVFLTHEKTFVRKLKEVAIALQIEQKYTKQQILEMYMNHIYFGHGAYGIQSAAKMYF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
NKNVEDLTVEEGAMLAGLLKAPSGYSPFLHPKKSKERRDLVLSLMHNQGYITAEESVRYQ
CCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCEEC
GKTIALYKNLDKQELAYMPYVDMVVDEAARLYGLSHQEVLRGGYTFVVSMDENMQKVAYD
CCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHCCCEEEEEECCCCHHHHHHH
QFQDEKNFPGGDKNVQGAFLLMDNQTGGIKAAIGGREYVPRGFNRVFAKRQPGSVLKPLL
HHHCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHH
VYAPALETKKYNPYSLLTNERTAFKGYVPRNYNHQYSKEVTMYDALLQSANVPAVSLLHE
HHCCCCCCCCCCCCEEECCCCHHEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
LGVEEGKQYLEKGNIHIADSGLSAALGGLKEGVSPFEIVKMYRAFLANGKIIEPHVIEKV
HCHHHHHHHHHHCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECHHHHHHH
FDRQGKMIGESQKEETKIFSKQTAWYMTKMLEGVVEEGTARAGVYKGALAGKTGTTSLSN
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHCCCCCCCCCCCCCH
QREGVRDIWFVGYTPSLVGAVWMGYDRTDKKHTLYGGSESATELFKKILMKAHVQDKEKF
HCCCCCEEEEECCCHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHH
KRPKGVATISKPIHLSKIEHLEAKISFHPFGLFIAKLSWKPLPDKRVMYRIYQIENGVHT
CCCCCCEECCCCCHHHHHHHHHHEEEECHHEEEEEEECCCCCCCHHHHHHHHHHCCCCEE
HIGTVKGTGEYDVKFINIFAKPSFYVVPYNSQTNREGEKTKVAKP
ECCCEECCCCCCEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]