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The map label for this gene is dmlA [H]

Identifier: 152974583

GI number: 152974583

Start: 877466

End: 878560

Strand: Direct

Name: dmlA [H]

Synonym: Bcer98_0765

Alternate gene names: 152974583

Gene position: 877466-878560 (Clockwise)

Preceding gene: 152974582

Following gene: 152974584

Centisome position: 21.47

GC content: 39.18

Gene sequence:

>1095_bases
ATGAAAACATTGAAAGTAGCTGTTATTGCTGGAGACGGAATTGGTCCTGAGGTAATGGATGAAGGTGTGAAAGTACTGCA
AACAATTGCAAATGTAAGTCAGCAATTTAAGTTTGAATTTACGTATTTTCCATGGGGGTGCGAATTTTATTCCAAGCATG
GAAAAATGATGGATGATGATGGGATTGAACAACTTAAGGCATTTGATGCGATTTATTTAGGTGCTGTAGGATTTCCTGGA
GTACCTGATTATATCTCGCTTTGGGATTTGCTTTTGAGAATTCGTCAAAGTTTCGATCAATATGTAAATATACGTCCTGT
CACTTTATTAAAAGGTGCTCCTTGCCCGTTAAAAGATGTAAAGCGTGAAGATATTGATATGCTCTTTATTCGGGAAAATA
GTGAAGGAGAGTATGCGGGTGCCGGCGATTGGTTATATAAAGGGAAAGAACATGAGGTCGTTTTACAAAATAGTGTGTTT
TCTCGTAAAGGAACGGAGCGCATTATTCGTTATGCATTTGAAATAGCTCGAAAGGAAAGGAAATCTTTAACGAGCATTAG
TAAAGGAAATGCCCTGAATTATTCAATGGTTTTTTGGGATCAAATTTTTGAAGAGATTAGTAAAGAATATCCGGATGTGA
AAACAGCTTCTTATTTAGTAGATGCAGCTGCAATGTTGATGATTAAAGAGCCGCACCGGTTTGAAGTTGTTGTGACATCG
AACTTATTTGGTGATATTTTAACGGACTTAGGGGCGGCTCTTGCCGGTGGTTTAGGCTTGGCAGCAGGAGCGAATATTAA
TCCTGAAAGACAGTATCCATCGATGTTTGAGCCAATTCATGGTTCGGCGCCCGATATTGCAGGGCAACAAATCGCCAATC
CTTTGGCGGCAATATGGTCAGCAAGCCAAATGCTCGATTTTTTCGGATATGAATCTTACGGAAAATTAGTATTAGATGCA
TTGGAACAGACTTTGGTAGAAGGGAAAGTTTTGACACCAGATATGGGGGGAACTGCTTCTACATCAGATGTTGGAAAACG
TATAGTGGAGATTTTAACATCTCTTATTCTTACCCAAACGCATGAAAGAGTGTAA

Upstream 100 bases:

>100_bases
AAGGAATAAAGTATGCCGTTGAAGAAATGACAGAATTGAAATTAATTGTTTGGAATCAAAACGAATGCGGTAGTTAATTT
AAGGGGAAGAAGGGTTTTTC

Downstream 100 bases:

>100_bases
GTAGAGAGGGATGCTTCCATACTCGGCAGGGGATATGAAAAATTTCTTGCCGAGTTTATGTCATTCTTTTTATAGAAAGA
CAATGAATCTATTGAATGTA

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKTLKVAVIAGDGIGPEVMDEGVKVLQTIANVSQQFKFEFTYFPWGCEFYSKHGKMMDDDGIEQLKAFDAIYLGAVGFPG
VPDYISLWDLLLRIRQSFDQYVNIRPVTLLKGAPCPLKDVKREDIDMLFIRENSEGEYAGAGDWLYKGKEHEVVLQNSVF
SRKGTERIIRYAFEIARKERKSLTSISKGNALNYSMVFWDQIFEEISKEYPDVKTASYLVDAAAMLMIKEPHRFEVVVTS
NLFGDILTDLGAALAGGLGLAAGANINPERQYPSMFEPIHGSAPDIAGQQIANPLAAIWSASQMLDFFGYESYGKLVLDA
LEQTLVEGKVLTPDMGGTASTSDVGKRIVEILTSLILTQTHERV

Sequences:

>Translated_364_residues
MKTLKVAVIAGDGIGPEVMDEGVKVLQTIANVSQQFKFEFTYFPWGCEFYSKHGKMMDDDGIEQLKAFDAIYLGAVGFPG
VPDYISLWDLLLRIRQSFDQYVNIRPVTLLKGAPCPLKDVKREDIDMLFIRENSEGEYAGAGDWLYKGKEHEVVLQNSVF
SRKGTERIIRYAFEIARKERKSLTSISKGNALNYSMVFWDQIFEEISKEYPDVKTASYLVDAAAMLMIKEPHRFEVVVTS
NLFGDILTDLGAALAGGLGLAAGANINPERQYPSMFEPIHGSAPDIAGQQIANPLAAIWSASQMLDFFGYESYGKLVLDA
LEQTLVEGKVLTPDMGGTASTSDVGKRIVEILTSLILTQTHERV
>Mature_364_residues
MKTLKVAVIAGDGIGPEVMDEGVKVLQTIANVSQQFKFEFTYFPWGCEFYSKHGKMMDDDGIEQLKAFDAIYLGAVGFPG
VPDYISLWDLLLRIRQSFDQYVNIRPVTLLKGAPCPLKDVKREDIDMLFIRENSEGEYAGAGDWLYKGKEHEVVLQNSVF
SRKGTERIIRYAFEIARKERKSLTSISKGNALNYSMVFWDQIFEEISKEYPDVKTASYLVDAAAMLMIKEPHRFEVVVTS
NLFGDILTDLGAALAGGLGLAAGANINPERQYPSMFEPIHGSAPDIAGQQIANPLAAIWSASQMLDFFGYESYGKLVLDA
LEQTLVEGKVLTPDMGGTASTSDVGKRIVEILTSLILTQTHERV

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=351, Percent_Identity=32.7635327635328, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI4758582, Length=352, Percent_Identity=29.5454545454545, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI28178821, Length=360, Percent_Identity=29.1666666666667, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI28178816, Length=358, Percent_Identity=28.7709497206704, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI28178838, Length=343, Percent_Identity=28.8629737609329, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI28178819, Length=194, Percent_Identity=32.9896907216495, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1788101, Length=360, Percent_Identity=50.8333333333333, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI87081683, Length=354, Percent_Identity=37.2881355932203, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1787381, Length=397, Percent_Identity=28.7153652392947, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71986051, Length=362, Percent_Identity=31.2154696132597, Blast_Score=167, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI25144293, Length=358, Percent_Identity=30.4469273743017, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17505779, Length=348, Percent_Identity=30.7471264367816, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17550882, Length=355, Percent_Identity=29.0140845070423, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324291, Length=360, Percent_Identity=35.8333333333333, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322097, Length=360, Percent_Identity=35.2777777777778, Blast_Score=182, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6319830, Length=361, Percent_Identity=32.6869806094183, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6324709, Length=355, Percent_Identity=32.3943661971831, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24643268, Length=358, Percent_Identity=32.9608938547486, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24643270, Length=358, Percent_Identity=32.9608938547486, Blast_Score=174, Evalue=9e-44,
Organism=Drosophila melanogaster, GI161078635, Length=267, Percent_Identity=33.7078651685393, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI161078637, Length=267, Percent_Identity=33.7078651685393, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI161078633, Length=267, Percent_Identity=33.7078651685393, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24650122, Length=267, Percent_Identity=33.7078651685393, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI161078639, Length=267, Percent_Identity=33.7078651685393, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24661184, Length=350, Percent_Identity=28, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI281362242, Length=353, Percent_Identity=28.328611898017, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24648872, Length=353, Percent_Identity=28.328611898017, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI20130355, Length=354, Percent_Identity=26.271186440678, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 40422; Mature: 40422

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLKVAVIAGDGIGPEVMDEGVKVLQTIANVSQQFKFEFTYFPWGCEFYSKHGKMMDDD
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHCCCCCCCC
GIEQLKAFDAIYLGAVGFPGVPDYISLWDLLLRIRQSFDQYVNIRPVTLLKGAPCPLKDV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
KREDIDMLFIRENSEGEYAGAGDWLYKGKEHEVVLQNSVFSRKGTERIIRYAFEIARKER
CCCCCCEEEEEECCCCCCCCCCCHHCCCCCCCEEECCHHHHHCHHHHHHHHHHHHHHHHH
KSLTSISKGNALNYSMVFWDQIFEEISKEYPDVKTASYLVDAAAMLMIKEPHRFEVVVTS
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEC
NLFGDILTDLGAALAGGLGLAAGANINPERQYPSMFEPIHGSAPDIAGQQIANPLAAIWS
HHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHH
ASQMLDFFGYESYGKLVLDALEQTLVEGKVLTPDMGGTASTSDVGKRIVEILTSLILTQT
HHHHHHHHCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
HERV
HCCC
>Mature Secondary Structure
MKTLKVAVIAGDGIGPEVMDEGVKVLQTIANVSQQFKFEFTYFPWGCEFYSKHGKMMDDD
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHCCCCCCCC
GIEQLKAFDAIYLGAVGFPGVPDYISLWDLLLRIRQSFDQYVNIRPVTLLKGAPCPLKDV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
KREDIDMLFIRENSEGEYAGAGDWLYKGKEHEVVLQNSVFSRKGTERIIRYAFEIARKER
CCCCCCEEEEEECCCCCCCCCCCHHCCCCCCCEEECCHHHHHCHHHHHHHHHHHHHHHHH
KSLTSISKGNALNYSMVFWDQIFEEISKEYPDVKTASYLVDAAAMLMIKEPHRFEVVVTS
HHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEC
NLFGDILTDLGAALAGGLGLAAGANINPERQYPSMFEPIHGSAPDIAGQQIANPLAAIWS
HHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHH
ASQMLDFFGYESYGKLVLDALEQTLVEGKVLTPDMGGTASTSDVGKRIVEILTSLILTQT
HHHHHHHHCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
HERV
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]