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The map label for this gene is yhaR [H]

Identifier: 152974544

GI number: 152974544

Start: 828479

End: 829267

Strand: Direct

Name: yhaR [H]

Synonym: Bcer98_0721

Alternate gene names: 152974544

Gene position: 828479-829267 (Clockwise)

Preceding gene: 152974542

Following gene: 152974545

Centisome position: 20.27

GC content: 38.91

Gene sequence:

>789_bases
ATGGAAGTAAAAAGCAAAGCAGAATCTGTTGTTGTGAAATATGAAGGACGTGTTGCAACAGTGATGGTCAATCGTCCTGA
AGTATTAAATGCGTTAGATGAGCCTACCTTAAGAGAATTACGAAAAAAACTGAAAGAAGTGGCAGAAAGTCCTGCTCACA
TTGTCGTATTATGCGGAAATGGCCGTGGTTTTTCTGCAGGTGGGGATATTAAATCCATGCTTTCTAGCAACGATGAAAGT
AAATTTGACGACATTATGAATACGATTTCGGAAATTGTTATTACGTTATATACAATGCCAAAGCTTGTGATTAGTGCAAT
TCATGGTCCAACGGCAGGCCTTGGACTAAGCATTGCGTTAACAGCGGATTATGTGCTAGCAGATATTTCATCTATTATTG
CGATGAATTTCATTGGAATTGCGCTGATTCCAGATGGCGGAGGACATTTCTTCTTACAAAAACGACTTGGTGAAAATGTG
GCCAAGCAAATCATTTGGGAAGGGAAAAAACTATCGGCAACGGAAGCGTTAGATCTCGGTTTAATTGATGAAGTAATCGG
GGAAAACTTCCAAGGGGCTGTAAAACAAAAAATCAATGAGTGGTTGCAAAAGCCAATTCAAGCAATGATTCAAACGAAAC
AAATTTTATGTGAATTCAATCGCTCAAGTCTGGAACGAACACTAACACTTGAAAAGCGTGGACAATATGCAATGAGAAAA
ACAGCCGATCATAAAGAGGGAATTGCTGCGTTTTTAGAAAAGCGTTCCCCAATCTTTAAAGGAGAATAA

Upstream 100 bases:

>100_bases
TTGCCTTTATTAGACAGAAAATTAGGGAAAAAGAAGGGTTTTGTAGCTCTTTTCTATAAGTATAGAAGTATAATCATTGG
ACAAGAGAGAGGGGTATATA

Downstream 100 bases:

>100_bases
AAAGGAAGAAAGTGCTAGCACATTTTAGTATAATTGTGTTAGCACTTTTTTAGGAGAAATAGATGAAAAAATAAAAGAAA
AAGTATAATGGTGATTACGC

Product: enoyl-CoA hydratase

Products: crotonoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MEVKSKAESVVVKYEGRVATVMVNRPEVLNALDEPTLRELRKKLKEVAESPAHIVVLCGNGRGFSAGGDIKSMLSSNDES
KFDDIMNTISEIVITLYTMPKLVISAIHGPTAGLGLSIALTADYVLADISSIIAMNFIGIALIPDGGGHFFLQKRLGENV
AKQIIWEGKKLSATEALDLGLIDEVIGENFQGAVKQKINEWLQKPIQAMIQTKQILCEFNRSSLERTLTLEKRGQYAMRK
TADHKEGIAAFLEKRSPIFKGE

Sequences:

>Translated_262_residues
MEVKSKAESVVVKYEGRVATVMVNRPEVLNALDEPTLRELRKKLKEVAESPAHIVVLCGNGRGFSAGGDIKSMLSSNDES
KFDDIMNTISEIVITLYTMPKLVISAIHGPTAGLGLSIALTADYVLADISSIIAMNFIGIALIPDGGGHFFLQKRLGENV
AKQIIWEGKKLSATEALDLGLIDEVIGENFQGAVKQKINEWLQKPIQAMIQTKQILCEFNRSSLERTLTLEKRGQYAMRK
TADHKEGIAAFLEKRSPIFKGE
>Mature_262_residues
MEVKSKAESVVVKYEGRVATVMVNRPEVLNALDEPTLRELRKKLKEVAESPAHIVVLCGNGRGFSAGGDIKSMLSSNDES
KFDDIMNTISEIVITLYTMPKLVISAIHGPTAGLGLSIALTADYVLADISSIIAMNFIGIALIPDGGGHFFLQKRLGENV
AKQIIWEGKKLSATEALDLGLIDEVIGENFQGAVKQKINEWLQKPIQAMIQTKQILCEFNRSSLERTLTLEKRGQYAMRK
TADHKEGIAAFLEKRSPIFKGE

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=270, Percent_Identity=31.1111111111111, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI260274832, Length=222, Percent_Identity=26.5765765765766, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI45643119, Length=222, Percent_Identity=26.5765765765766, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI260275230, Length=222, Percent_Identity=26.5765765765766, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI116235487, Length=220, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI4757966, Length=224, Percent_Identity=26.7857142857143, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI51538581, Length=224, Percent_Identity=26.7857142857143, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI25453481, Length=224, Percent_Identity=26.7857142857143, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI51538573, Length=224, Percent_Identity=26.7857142857143, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4757968, Length=224, Percent_Identity=26.7857142857143, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI49355787, Length=224, Percent_Identity=26.7857142857143, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI68989263, Length=212, Percent_Identity=27.3584905660377, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4502327, Length=206, Percent_Identity=31.0679611650485, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI221307494, Length=192, Percent_Identity=26.0416666666667, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI221136756, Length=192, Percent_Identity=26.0416666666667, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221136753, Length=192, Percent_Identity=26.0416666666667, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=21.259842519685, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI37594471, Length=168, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI37594469, Length=168, Percent_Identity=27.9761904761905, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=28.2352941176471, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=32.3076923076923, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI221142681, Length=267, Percent_Identity=25.0936329588015, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=24.9027237354086, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=174, Percent_Identity=26.4367816091954, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=24.4, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790281, Length=201, Percent_Identity=26.865671641791, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=32.258064516129, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=255, Percent_Identity=31.7647058823529, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=25, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=276, Percent_Identity=26.8115942028986, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=276, Percent_Identity=26.8115942028986, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=188, Percent_Identity=28.7234042553192, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17536985, Length=199, Percent_Identity=26.1306532663317, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20129971, Length=255, Percent_Identity=29.0196078431373, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653477, Length=255, Percent_Identity=29.0196078431373, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653139, Length=253, Percent_Identity=26.4822134387352, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24654903, Length=210, Percent_Identity=23.3333333333333, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI28571729, Length=160, Percent_Identity=28.125, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28571730, Length=160, Percent_Identity=28.125, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.55

Molecular weight: Translated: 28800; Mature: 28800

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKSKAESVVVKYEGRVATVMVNRPEVLNALDEPTLRELRKKLKEVAESPAHIVVLCGN
CCCCCCCCEEEEEECCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECC
GRGFSAGGDIKSMLSSNDESKFDDIMNTISEIVITLYTMPKLVISAIHGPTAGLGLSIAL
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEH
TADYVLADISSIIAMNFIGIALIPDGGGHFFLQKRLGENVAKQIIWEGKKLSATEALDLG
HHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LIDEVIGENFQGAVKQKINEWLQKPIQAMIQTKQILCEFNRSSLERTLTLEKRGQYAMRK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHH
TADHKEGIAAFLEKRSPIFKGE
HCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEVKSKAESVVVKYEGRVATVMVNRPEVLNALDEPTLRELRKKLKEVAESPAHIVVLCGN
CCCCCCCCEEEEEECCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECC
GRGFSAGGDIKSMLSSNDESKFDDIMNTISEIVITLYTMPKLVISAIHGPTAGLGLSIAL
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEH
TADYVLADISSIIAMNFIGIALIPDGGGHFFLQKRLGENVAKQIIWEGKKLSATEALDLG
HHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LIDEVIGENFQGAVKQKINEWLQKPIQAMIQTKQILCEFNRSSLERTLTLEKRGQYAMRK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHH
TADHKEGIAAFLEKRSPIFKGE
HCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxybutanoyl-CoA

Specific reaction: (3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 9579061 [H]