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The map label for this gene is thiF [H]

Identifier: 152974445

GI number: 152974445

Start: 709872

End: 710891

Strand: Direct

Name: thiF [H]

Synonym: Bcer98_0620

Alternate gene names: 152974445

Gene position: 709872-710891 (Clockwise)

Preceding gene: 152974444

Following gene: 152974446

Centisome position: 17.37

GC content: 38.53

Gene sequence:

>1020_bases
TTGAATAATCGATATTCTCGTCAAGAGCTATTCTCCTCAATTGGAGAAAAAGGGCAAAAAAAGATAGGGAAAAAGCATGT
ATTCATTATCGGTGCAGGAGCACTTGGTAGCGCCAATGCAGAAATGCTTGTAAGAGCGGGTGTGGGAAATGTAACGATTG
TAGACCGCGACTATATTGATTGGAGTAATCTGCAAAGGCAGCAACTATACGCTGAAGAAGATGTGAAGAACCATCTCCCG
AAAGCGATCGCGGCAAAACAGCGTTTGAAAGCGATTAATAGTAGTGTAGTGGTAAAGGCTTTTGTCAAAGATATAACCGC
AGAAGAGCTAGAAGAACTTGTTACAAATGTCGATGTCATCATCGATGCGACTGATAATTTTGAAACACGCTTTATTATAA
ATGATATATCACAAAAATATTCAATTCCATGGATTTACGGTGCTTGTGTAGGGAGCTATGGTCTTTCTTATACCATTTTA
CCAGGAAAGACACCGTGTTTATCTTGCTTACTACAATCCATCCCACTTGGCGGAGCAACATGTGATACAGCAGGCATTAT
TTCACCTGCTGTATCGATCGTTGTTTCGCATCAAGTAACAGAAACTCTTAAACTTTTAGTAGAAGATTATAAAGCGCTTC
GAGATGGTCTCGTGTCATTTGATGTATGGAAAAATGAATATTCCTGTATGAATGTACAGAAGTTGAAAAAGGATCATTGT
CCATCTTGTGGTGAGAAAGCAATCTATCCATATTTAAATAAAGAGAATACGTCAAAAACGGCTGTTTTATGCGGGAGAAA
TACAGTTCAAATTCGTCCGCCTCATAAAGAAAAACTGGACTTTCATAGATATAAAGAACTGTTACGTGACCGCGTAGCTG
ATTTAACAGTGAACCCATATTTATTATCATTTCGTGCAGATGGAAAACGGCTTGTTGCTTTTCAAGACGGACGAGTTCTT
GTGTATGGCACGAAAGAGATAGCAGAAGCGAAAACAATATATCATCGTTATTTTGGATAA

Upstream 100 bases:

>100_bases
AAACTAGGTATTCATGCAGGACGTTTAGGATTTGAAGCAGGACGTATTGCACGGAAACGCTTTGCAACAGCAAGCAGTCC
TTTAGAAGGAATGAGCGTAG

Downstream 100 bases:

>100_bases
AAGGAGTGAATGAGATGAAAGTAAATAAAGCATTAACAATTGCTGGATCTGATAGCGGTGGCGGCGCTGGAATTCAAGCG
GATTTGAAAACATTCCAAGA

Product: thiamine/molybdopterin biosynthesis ThiF/MoeB-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNNRYSRQELFSSIGEKGQKKIGKKHVFIIGAGALGSANAEMLVRAGVGNVTIVDRDYIDWSNLQRQQLYAEEDVKNHLP
KAIAAKQRLKAINSSVVVKAFVKDITAEELEELVTNVDVIIDATDNFETRFIINDISQKYSIPWIYGACVGSYGLSYTIL
PGKTPCLSCLLQSIPLGGATCDTAGIISPAVSIVVSHQVTETLKLLVEDYKALRDGLVSFDVWKNEYSCMNVQKLKKDHC
PSCGEKAIYPYLNKENTSKTAVLCGRNTVQIRPPHKEKLDFHRYKELLRDRVADLTVNPYLLSFRADGKRLVAFQDGRVL
VYGTKEIAEAKTIYHRYFG

Sequences:

>Translated_339_residues
MNNRYSRQELFSSIGEKGQKKIGKKHVFIIGAGALGSANAEMLVRAGVGNVTIVDRDYIDWSNLQRQQLYAEEDVKNHLP
KAIAAKQRLKAINSSVVVKAFVKDITAEELEELVTNVDVIIDATDNFETRFIINDISQKYSIPWIYGACVGSYGLSYTIL
PGKTPCLSCLLQSIPLGGATCDTAGIISPAVSIVVSHQVTETLKLLVEDYKALRDGLVSFDVWKNEYSCMNVQKLKKDHC
PSCGEKAIYPYLNKENTSKTAVLCGRNTVQIRPPHKEKLDFHRYKELLRDRVADLTVNPYLLSFRADGKRLVAFQDGRVL
VYGTKEIAEAKTIYHRYFG
>Mature_339_residues
MNNRYSRQELFSSIGEKGQKKIGKKHVFIIGAGALGSANAEMLVRAGVGNVTIVDRDYIDWSNLQRQQLYAEEDVKNHLP
KAIAAKQRLKAINSSVVVKAFVKDITAEELEELVTNVDVIIDATDNFETRFIINDISQKYSIPWIYGACVGSYGLSYTIL
PGKTPCLSCLLQSIPLGGATCDTAGIISPAVSIVVSHQVTETLKLLVEDYKALRDGLVSFDVWKNEYSCMNVQKLKKDHC
PSCGEKAIYPYLNKENTSKTAVLCGRNTVQIRPPHKEKLDFHRYKELLRDRVADLTVNPYLLSFRADGKRLVAFQDGRVL
VYGTKEIAEAKTIYHRYFG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=288, Percent_Identity=27.4305555555556, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI38045948, Length=172, Percent_Identity=29.6511627906977, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1787048, Length=244, Percent_Identity=29.9180327868852, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=33.7349397590361, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17540406, Length=245, Percent_Identity=32.2448979591837, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI193203301, Length=154, Percent_Identity=30.5194805194805, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=303, Percent_Identity=26.4026402640264, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320598, Length=188, Percent_Identity=32.9787234042553, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6325323, Length=160, Percent_Identity=33.125, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322639, Length=198, Percent_Identity=27.7777777777778, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582879, Length=247, Percent_Identity=26.3157894736842, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28573937, Length=176, Percent_Identity=30.6818181818182, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24660640, Length=152, Percent_Identity=28.9473684210526, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 37945; Mature: 37945

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRYSRQELFSSIGEKGQKKIGKKHVFIIGAGALGSANAEMLVRAGVGNVTIVDRDYID
CCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCC
WSNLQRQQLYAEEDVKNHLPKAIAAKQRLKAINSSVVVKAFVKDITAEELEELVTNVDVI
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEE
IDATDNFETRFIINDISQKYSIPWIYGACVGSYGLSYTILPGKTPCLSCLLQSIPLGGAT
EECCCCCCEEEEEEHHHHHCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCC
CDTAGIISPAVSIVVSHQVTETLKLLVEDYKALRDGLVSFDVWKNEYSCMNVQKLKKDHC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCCCCCHHHHHHHCC
PSCGEKAIYPYLNKENTSKTAVLCGRNTVQIRPPHKEKLDFHRYKELLRDRVADLTVNPY
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCHHHCCHHHHHHHHHHHHHCCCCCCE
LLSFRADGKRLVAFQDGRVLVYGTKEIAEAKTIYHRYFG
EEEEECCCCEEEEEECCEEEEEECHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNRYSRQELFSSIGEKGQKKIGKKHVFIIGAGALGSANAEMLVRAGVGNVTIVDRDYID
CCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCC
WSNLQRQQLYAEEDVKNHLPKAIAAKQRLKAINSSVVVKAFVKDITAEELEELVTNVDVI
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEE
IDATDNFETRFIINDISQKYSIPWIYGACVGSYGLSYTILPGKTPCLSCLLQSIPLGGAT
EECCCCCCEEEEEEHHHHHCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCC
CDTAGIISPAVSIVVSHQVTETLKLLVEDYKALRDGLVSFDVWKNEYSCMNVQKLKKDHC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCCCCCHHHHHHHCC
PSCGEKAIYPYLNKENTSKTAVLCGRNTVQIRPPHKEKLDFHRYKELLRDRVADLTVNPY
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCHHHCCHHHHHHHHHHHHHCCCCCCE
LLSFRADGKRLVAFQDGRVLVYGTKEIAEAKTIYHRYFG
EEEEECCCCEEEEEECCEEEEEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]