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The map label for this gene is brnQ [H]

Identifier: 152974408

GI number: 152974408

Start: 660719

End: 662014

Strand: Direct

Name: brnQ [H]

Synonym: Bcer98_0582

Alternate gene names: 152974408

Gene position: 660719-662014 (Clockwise)

Preceding gene: 152974406

Following gene: 152974419

Centisome position: 16.17

GC content: 37.89

Gene sequence:

>1296_bases
GTGTTACAGAAAAAAGAGATTTTACTTATTAGCTTAATGCTATTCTCCATGTTCTTTGGTGCTGGAAATCTTATATTCCC
TCCCTTTTTAGGATATGAGGCAGGAGAGTATGTGTGGATTTCGTTGATAGGTTTTATCATATCAGCGACAGGACTTCCGA
TATTAGGTGTTGTGGCAGTTGCGAAGGCAGGAAGTTTTCAAGCATTAGGAGAACGGGTGCATCGTTCATTTGCTATTGTT
TTTCCTTGTTTAGTGTATGTATTTATCGGTCCAGGACTTGGAATACCACGAGCAGGGAGTTTAGCGTTTGAAATGGGATT
CGGATCATTCTTTCCGGAGCCTGGAAAATTAGTCTTGTTAGTTTACACGGTCGTTTTCTTTAGTATTGTGTATTGGCTTA
GCTTATCGCCATCAAGACTAATGGACCTATTTGGGAAAGTGTTGACGCCTTTATTATTATGTATGATTGCTCTTATTTTT
ATAAAAAGCATGATTACGCCTGTAGGAGCTTTGGAAGCGCCGCTTGGAAATTATGGACAAGCACCTATGTTTCAAGGATT
TTTAGATGGCTATTTAACAATGGATGCATTGGCTGCCTTAATCTTTGGTATTGTAGTTGCGAATGCTGTACAGGAAAAGG
GGATAAAATCCGATAAAAGCTTAGCGAAATATATGAGTATAGCGGGCGTTGGTGCCGGGCTTTTATTGTCTATTATTTAT
ATTATCCTTGGATATGTCGGTTCAATTAGTGGTTCATTAGGACAGTTTGAGAATGGTGCGAAAGTACTAGCAGGAGTGAT
GACTCTGTTATTTGGACAAAGTGGTGTTGTTCTGCTAGGAATTATCTTTACGATTGCATGCCTCTGTGTTTCCGTAGGTC
TTGTGACATCGTGTAGTCAATTTTTTACAAGTGTATTCCCCAAAATACCTTATAGAGTTTGGGGATTTATACTAAGCTTT
ATAAGCATGTTTCTGGCTAATTTGGGATTGACAGAGATTTTAAAGGTATCGGTTCCAATCCTTGGATTCATATATCCAGT
TGCTTTAACGTTAATCATATTAGGACTGTTTCATAAAAAGATTAGCCGATATGTATATATATATCCTATAACAATCGGGA
GCGTAGCGCTATTTAGCGCAATAGATATTGTAAATCGGAATGTATTCATGGATAGATGGACACCGCTACTTAAACATATT
CCTTTTTATACAGAAGGAGTCGGTTGGCTAGTTCCGGCTATTGTAAGTGCGTGTCTTGGAATTGTTATAAGTGTTTTAGT
TAACAAAAAGGATTAA

Upstream 100 bases:

>100_bases
AAGAAATCGGAAAACATAACAAGTGTAGAATGAGAAATTCTTTTGTGATAGACTATGGACTATGTTTGCTTTATATGGAA
TTTAGAAGGAGAAAATGAGA

Downstream 100 bases:

>100_bases
AAAGGTGCTTTCCATAACGGAAAACACCTTTTTATTTAGTTTGCATGAATTGTAACAGGCTCGCTATATTTAGCTATAGA
ATTTTGACGTAGGCGCAATG

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MLQKKEILLISLMLFSMFFGAGNLIFPPFLGYEAGEYVWISLIGFIISATGLPILGVVAVAKAGSFQALGERVHRSFAIV
FPCLVYVFIGPGLGIPRAGSLAFEMGFGSFFPEPGKLVLLVYTVVFFSIVYWLSLSPSRLMDLFGKVLTPLLLCMIALIF
IKSMITPVGALEAPLGNYGQAPMFQGFLDGYLTMDALAALIFGIVVANAVQEKGIKSDKSLAKYMSIAGVGAGLLLSIIY
IILGYVGSISGSLGQFENGAKVLAGVMTLLFGQSGVVLLGIIFTIACLCVSVGLVTSCSQFFTSVFPKIPYRVWGFILSF
ISMFLANLGLTEILKVSVPILGFIYPVALTLIILGLFHKKISRYVYIYPITIGSVALFSAIDIVNRNVFMDRWTPLLKHI
PFYTEGVGWLVPAIVSACLGIVISVLVNKKD

Sequences:

>Translated_431_residues
MLQKKEILLISLMLFSMFFGAGNLIFPPFLGYEAGEYVWISLIGFIISATGLPILGVVAVAKAGSFQALGERVHRSFAIV
FPCLVYVFIGPGLGIPRAGSLAFEMGFGSFFPEPGKLVLLVYTVVFFSIVYWLSLSPSRLMDLFGKVLTPLLLCMIALIF
IKSMITPVGALEAPLGNYGQAPMFQGFLDGYLTMDALAALIFGIVVANAVQEKGIKSDKSLAKYMSIAGVGAGLLLSIIY
IILGYVGSISGSLGQFENGAKVLAGVMTLLFGQSGVVLLGIIFTIACLCVSVGLVTSCSQFFTSVFPKIPYRVWGFILSF
ISMFLANLGLTEILKVSVPILGFIYPVALTLIILGLFHKKISRYVYIYPITIGSVALFSAIDIVNRNVFMDRWTPLLKHI
PFYTEGVGWLVPAIVSACLGIVISVLVNKKD
>Mature_431_residues
MLQKKEILLISLMLFSMFFGAGNLIFPPFLGYEAGEYVWISLIGFIISATGLPILGVVAVAKAGSFQALGERVHRSFAIV
FPCLVYVFIGPGLGIPRAGSLAFEMGFGSFFPEPGKLVLLVYTVVFFSIVYWLSLSPSRLMDLFGKVLTPLLLCMIALIF
IKSMITPVGALEAPLGNYGQAPMFQGFLDGYLTMDALAALIFGIVVANAVQEKGIKSDKSLAKYMSIAGVGAGLLLSIIY
IILGYVGSISGSLGQFENGAKVLAGVMTLLFGQSGVVLLGIIFTIACLCVSVGLVTSCSQFFTSVFPKIPYRVWGFILSF
ISMFLANLGLTEILKVSVPILGFIYPVALTLIILGLFHKKISRYVYIYPITIGSVALFSAIDIVNRNVFMDRWTPLLKHI
PFYTEGVGWLVPAIVSACLGIVISVLVNKKD

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=434, Percent_Identity=34.7926267281106, Blast_Score=263, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46539; Mature: 46539

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]