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The map label for this gene is sph [H]

Identifier: 152974395

GI number: 152974395

Start: 649035

End: 650027

Strand: Direct

Name: sph [H]

Synonym: Bcer98_0568

Alternate gene names: 152974395

Gene position: 649035-650027 (Clockwise)

Preceding gene: 152974394

Following gene: 152974396

Centisome position: 15.88

GC content: 34.14

Gene sequence:

>993_bases
ATGATGAAAAGTAAGTTGTTAAAAGGTATTCTTAGCTTTGGAATTGGTTTGACAGCCTTATATAGTTCTTCTGCACAGGC
TGAAACATCTAGTGATAGCGATACATTCAAAATAATGACACATAATGTATATATGCTATCAACAAATTTGTATCCAAATT
GGGGACAAAAGGAGCGAGCAGACTTAATTGGGGAAGCGAATTATATAAAAAATCAAGATGTTGTTATATTAAATGAAGTA
TTTGATAATAAAGCTTCAGACAGTCTATTGCAAAATCTAAAGAAAGAGTATCCAAACCAAACAGCGGTATTAGGTCGCGT
TAATGGAGATATGTGGGATCAAACGTTAGGTAATTATTCTTCTGTAGCCCCTGAAGATGGAGGAGTTGCAATCGTAAGTA
AGTGGCCAATTGTGGAAAAGGTACAATATGTATTTCAGAAAGGATGCGGGCCAGACAATCTATCTAATAAAGGATTTGTC
TACACAAAAATTAAGAAAGATGATCGTTTTATTCACGTAATTGGAACGCACCTACAAGCAGAGGATAATCAGTGTGGAAA
CATTTCACCATCATCGGTGCGCACAAACCAATTAAAAGAAATTCAAGAGTTTATTCAAAAGAAAAATATTCCAAGTGACG
AGTATGTAGTCATTGGTGGAGATATGAATGTAAATAAAATAAACAGTAATCATACGTCAGAATATGAATCTATGTTTAAA
ACATTACATGCAAGTGTACCATCGTATACAGGACATACTGCAACTTGGGATGCAACAACAAATAGTATTGCAAAATACAA
CTTCCCAGAGGCACCAGCGGAATATTTAGACTACATCGTTACAAGTAAAGATCATGCGACTCCAACATATTTAGAGAATA
AAGTATTACAGCCAAAATCTCCTGAATGGTCTGTCACTTCATGGTTTAAAACATATACATATAATGATTATTCAGATCAT
TATCCAGTAGAGGCAACAATCATTATGAAATAA

Upstream 100 bases:

>100_bases
TCAACTTTGGTTTGACACATATGTAAACCGTTAAGAGTAATTTGAGAAGGCCAAATATGTTGTATTTGGTCTTTTCGAAT
ATAATAAATGGAGGTAGTGA

Downstream 100 bases:

>100_bases
GTGAAAAAAGTTTCTTCTTTGTGGAAGAAACTTTTTTATTTGGTTTGGTGACAGGAAAATAGAAGTTTCTCTCCAATATG
TATAAGACAAATAGAGAAGG

Product: sphingomyelin phosphodiesterase

Products: NA

Alternate protein names: SMase; Cereolysin B; SMPLC; Sphingomyelin phosphodiesterase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MMKSKLLKGILSFGIGLTALYSSSAQAETSSDSDTFKIMTHNVYMLSTNLYPNWGQKERADLIGEANYIKNQDVVILNEV
FDNKASDSLLQNLKKEYPNQTAVLGRVNGDMWDQTLGNYSSVAPEDGGVAIVSKWPIVEKVQYVFQKGCGPDNLSNKGFV
YTKIKKDDRFIHVIGTHLQAEDNQCGNISPSSVRTNQLKEIQEFIQKKNIPSDEYVVIGGDMNVNKINSNHTSEYESMFK
TLHASVPSYTGHTATWDATTNSIAKYNFPEAPAEYLDYIVTSKDHATPTYLENKVLQPKSPEWSVTSWFKTYTYNDYSDH
YPVEATIIMK

Sequences:

>Translated_330_residues
MMKSKLLKGILSFGIGLTALYSSSAQAETSSDSDTFKIMTHNVYMLSTNLYPNWGQKERADLIGEANYIKNQDVVILNEV
FDNKASDSLLQNLKKEYPNQTAVLGRVNGDMWDQTLGNYSSVAPEDGGVAIVSKWPIVEKVQYVFQKGCGPDNLSNKGFV
YTKIKKDDRFIHVIGTHLQAEDNQCGNISPSSVRTNQLKEIQEFIQKKNIPSDEYVVIGGDMNVNKINSNHTSEYESMFK
TLHASVPSYTGHTATWDATTNSIAKYNFPEAPAEYLDYIVTSKDHATPTYLENKVLQPKSPEWSVTSWFKTYTYNDYSDH
YPVEATIIMK
>Mature_330_residues
MMKSKLLKGILSFGIGLTALYSSSAQAETSSDSDTFKIMTHNVYMLSTNLYPNWGQKERADLIGEANYIKNQDVVILNEV
FDNKASDSLLQNLKKEYPNQTAVLGRVNGDMWDQTLGNYSSVAPEDGGVAIVSKWPIVEKVQYVFQKGCGPDNLSNKGFV
YTKIKKDDRFIHVIGTHLQAEDNQCGNISPSSVRTNQLKEIQEFIQKKNIPSDEYVVIGGDMNVNKINSNHTSEYESMFK
TLHASVPSYTGHTATWDATTNSIAKYNFPEAPAEYLDYIVTSKDHATPTYLENKVLQPKSPEWSVTSWFKTYTYNDYSDH
YPVEATIIMK

Specific function: Required, with sphingomyelinase, to effect target cell lysis (hemolysis) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the neutral sphingomyelinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005135
- InterPro:   IPR017766 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.4.12 [H]

Molecular weight: Translated: 37156; Mature: 37156

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKSKLLKGILSFGIGLTALYSSSAQAETSSDSDTFKIMTHNVYMLSTNLYPNWGQKERA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCHHHH
DLIGEANYIKNQDVVILNEVFDNKASDSLLQNLKKEYPNQTAVLGRVNGDMWDQTLGNYS
HHHCCCHHCCCCCEEEEHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCC
SVAPEDGGVAIVSKWPIVEKVQYVFQKGCGPDNLSNKGFVYTKIKKDDRFIHVIGTHLQA
CCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEECEEEC
EDNQCGNISPSSVRTNQLKEIQEFIQKKNIPSDEYVVIGGDMNVNKINSNHTSEYESMFK
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEECCCCCCHHHHHHHH
TLHASVPSYTGHTATWDATTNSIAKYNFPEAPAEYLDYIVTSKDHATPTYLENKVLQPKS
HHHHCCCCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCC
PEWSVTSWFKTYTYNDYSDHYPVEATIIMK
CCCCHHHHEEEEECCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MMKSKLLKGILSFGIGLTALYSSSAQAETSSDSDTFKIMTHNVYMLSTNLYPNWGQKERA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCHHHH
DLIGEANYIKNQDVVILNEVFDNKASDSLLQNLKKEYPNQTAVLGRVNGDMWDQTLGNYS
HHHCCCHHCCCCCEEEEHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCC
SVAPEDGGVAIVSKWPIVEKVQYVFQKGCGPDNLSNKGFVYTKIKKDDRFIHVIGTHLQA
CCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEECEEEC
EDNQCGNISPSSVRTNQLKEIQEFIQKKNIPSDEYVVIGGDMNVNKINSNHTSEYESMFK
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEECCCCCCHHHHHHHH
TLHASVPSYTGHTATWDATTNSIAKYNFPEAPAEYLDYIVTSKDHATPTYLENKVLQPKS
HHHHCCCCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCC
PEWSVTSWFKTYTYNDYSDHYPVEATIIMK
CCCCHHHHEEEEECCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2848222; 8387306; 3137122 [H]