Click here to switch to the map view.

The map label for this gene is glnH [H]

Identifier: 152974368

GI number: 152974368

Start: 619386

End: 620213

Strand: Direct

Name: glnH [H]

Synonym: Bcer98_0541

Alternate gene names: 152974368

Gene position: 619386-620213 (Clockwise)

Preceding gene: 152974367

Following gene: 152974369

Centisome position: 15.15

GC content: 35.51

Gene sequence:

>828_bases
ATGAAAAGGATGAAAAAATGGTTCACTCTTATTGTTTTTGTGTGCTTATTTACTTTGATTGTTGCAGGATGTGGAAATAA
ACAAGAAGAGGCGAAAGAAACAAGTACAAGTGGCGGAGTGGTCGAACAAATTAAGAAACGCGGAAAGTTAGTAGTTGGTG
TAAAGAATGATACTAATTTATTTGGATTAAAGAACCCTTCAACAGGGCAAGTAGAAGGGTTTGATGTTGATATGGCAAAA
GCACTTGCAAAAAGGATTCTTGGTGATGAGAAGAAACTAGAACTAAAAGAAGTAACTTCGAAAACGCGTATTCCAATGCT
GAAAAATGGAGATATTGATGCGATTATTGCGACAATGACAATTACAGAAGAACGGAAAAAAGAAGTGGATTTTTCAGATG
TATATTTTAAAGCAGGTCAATCATTGCTTGTGAAAAAAGGAAGCGATATTAAAAGTATTGATGATGTGAAAAAAGGGGTA
AAAGTATTAGCGGTAAAAGGATCCACATCAACACAAAATGTTCGTCAAAAAGCACCGGAGGCAACCGTATTAGAATTCGA
AAATTATAGTGAAGCATTTACAGCATTAAAGGCGGGACAGGGAGATGTCTTAACGACTGATAATGCAATTTTATATGGAA
TGGCCAAACAAGATGCGAATTATGAAGTAGTGGGTAAGATCTTTACGAATGAGCCATACGGCATTGCCGTTCAAAAAGGA
GCAACGGATTTAACGAAAGAGATCAATGATTTGTTAAAAGAAATAAAAGAAAATGGAGAATATGACAAGTTATACGAGAA
ATGGATTGGGGAAAAGCCAGAAAAATAG

Upstream 100 bases:

>100_bases
TATTGAGGATACAATACCAGCAGCGTTTTTTGTGAACCCAGAGCAGGAGAGAGCGAGACTGTTTTTAAGAAGAGTATTCA
ATCATTAGAGGAGGAGCATG

Downstream 100 bases:

>100_bases
TAGATTGCGAGGAGAGGGATGAGGTTGGCTCATCCTCTTCTTATAAAGAAAGAGGGTGGAAGTTTGCCTGACTTTTCAAT
TTTGACAAATAACATAGATT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKRMKKWFTLIVFVCLFTLIVAGCGNKQEEAKETSTSGGVVEQIKKRGKLVVGVKNDTNLFGLKNPSTGQVEGFDVDMAK
ALAKRILGDEKKLELKEVTSKTRIPMLKNGDIDAIIATMTITEERKKEVDFSDVYFKAGQSLLVKKGSDIKSIDDVKKGV
KVLAVKGSTSTQNVRQKAPEATVLEFENYSEAFTALKAGQGDVLTTDNAILYGMAKQDANYEVVGKIFTNEPYGIAVQKG
ATDLTKEINDLLKEIKENGEYDKLYEKWIGEKPEK

Sequences:

>Translated_275_residues
MKRMKKWFTLIVFVCLFTLIVAGCGNKQEEAKETSTSGGVVEQIKKRGKLVVGVKNDTNLFGLKNPSTGQVEGFDVDMAK
ALAKRILGDEKKLELKEVTSKTRIPMLKNGDIDAIIATMTITEERKKEVDFSDVYFKAGQSLLVKKGSDIKSIDDVKKGV
KVLAVKGSTSTQNVRQKAPEATVLEFENYSEAFTALKAGQGDVLTTDNAILYGMAKQDANYEVVGKIFTNEPYGIAVQKG
ATDLTKEINDLLKEIKENGEYDKLYEKWIGEKPEK
>Mature_275_residues
MKRMKKWFTLIVFVCLFTLIVAGCGNKQEEAKETSTSGGVVEQIKKRGKLVVGVKNDTNLFGLKNPSTGQVEGFDVDMAK
ALAKRILGDEKKLELKEVTSKTRIPMLKNGDIDAIIATMTITEERKKEVDFSDVYFKAGQSLLVKKGSDIKSIDDVKKGV
KVLAVKGSTSTQNVRQKAPEATVLEFENYSEAFTALKAGQGDVLTTDNAILYGMAKQDANYEVVGKIFTNEPYGIAVQKG
ATDLTKEINDLLKEIKENGEYDKLYEKWIGEKPEK

Specific function: Part of the ABC transporter complex glnHMPQ involved in glutamine transport [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=230, Percent_Identity=34.7826086956522, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786876, Length=244, Percent_Identity=29.0983606557377, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1788228, Length=235, Percent_Identity=33.6170212765957, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787088, Length=208, Percent_Identity=32.2115384615385, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787085, Length=210, Percent_Identity=29.0476190476191, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788649, Length=211, Percent_Identity=30.3317535545024, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788648, Length=213, Percent_Identity=30.5164319248826, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30432; Mature: 30432

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRMKKWFTLIVFVCLFTLIVAGCGNKQEEAKETSTSGGVVEQIKKRGKLVVGVKNDTNL
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCE
FGLKNPSTGQVEGFDVDMAKALAKRILGDEKKLELKEVTSKTRIPMLKNGDIDAIIATMT
EECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
ITEERKKEVDFSDVYFKAGQSLLVKKGSDIKSIDDVKKGVKVLAVKGSTSTQNVRQKAPE
ECHHHHHCCCHHHHHHHCCCEEEEECCCCCCHHHHHHCCEEEEEEECCCCHHHHHHHCCC
ATVLEFENYSEAFTALKAGQGDVLTTDNAILYGMAKQDANYEVVGKIFTNEPYGIAVQKG
CEEEEECCHHHHHHHHHCCCCCEEECCCEEEEEECCCCCCEEEEEEEECCCCCEEEEECC
ATDLTKEINDLLKEIKENGEYDKLYEKWIGEKPEK
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKRMKKWFTLIVFVCLFTLIVAGCGNKQEEAKETSTSGGVVEQIKKRGKLVVGVKNDTNL
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCE
FGLKNPSTGQVEGFDVDMAKALAKRILGDEKKLELKEVTSKTRIPMLKNGDIDAIIATMT
EECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
ITEERKKEVDFSDVYFKAGQSLLVKKGSDIKSIDDVKKGVKVLAVKGSTSTQNVRQKAPE
ECHHHHHCCCHHHHHHHCCCEEEEECCCCCCHHHHHHCCEEEEEEECCCCHHHHHHHCCC
ATVLEFENYSEAFTALKAGQGDVLTTDNAILYGMAKQDANYEVVGKIFTNEPYGIAVQKG
CEEEEECCHHHHHHHHHCCCCCEEECCCEEEEEECCCCCCEEEEEEEECCCCCEEEEECC
ATDLTKEINDLLKEIKENGEYDKLYEKWIGEKPEK
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 9384377 [H]