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The map label for this gene is malK [H]

Identifier: 152974327

GI number: 152974327

Start: 561915

End: 563501

Strand: Direct

Name: malK [H]

Synonym: Bcer98_0496

Alternate gene names: 152974327

Gene position: 561915-563501 (Clockwise)

Preceding gene: 152974326

Following gene: 152974328

Centisome position: 13.75

GC content: 34.15

Gene sequence:

>1587_bases
ATGAGAAATAGAAGGTTATTAAGTTTATGGAAAACGATTACACTGCTGGTATGCACTGTTGTTGTACTTTCTTTACTTGT
AACGGATATTTTAATTAGTCATCATGTTGAGCGTACGACAGAAGAAAGCCAAGCAGAAAAAGCCAAAATAATTGCGCGTA
TTGTAGCGCATTCACCAATTGTGTTGGAAGGCTTAACAGGTAGAAGAGATGCAGTTGAAATTCAAACATATACGAACAGG
TTATTAAAAAATACAGATGTTCAGTTTATTGTCGTTATGGATATGAAGGGAATTCGAAAATCTCATCCAAATCCTCAAAA
AATCGGTCATCATTTTGTTGGAGGGGATGAGGGAACGGTTTTAAAAGGAAAAGAACATGTGTCTTTAGCTGAAGGAACGT
TAGGGATTTCGATGCGCGTATTTGTACCTGTATTTTCTGAAGCAGGAAAGCAAGTTGGTGCAGTTGCTGTTGGGATTTCA
GCTGATAATGTAAAAGAGAGAACGAAAGAAAGTAGACATATTATTTATATTGGGATTAGCATAGGAATATTAGTTGGAAT
TATTGGGGCAGTGTTATTAGCCAGACATACTAAAAAGATTTTATTTGGCCTTGAACCACATGAAATTGCAAAAATTTTAG
AAGAAAGAAATACAATGCTACAATCTGTTAAAGAAGGAATTATTGCAGTAGATAAAGAAGCAAGAGTTACACTTATTAAT
AATGAAGCGAAGCGATTATTGAAAAAAAGTAGTCTTGAGGAAGAATGGATTGGTAAAGATGTTGAATCATATATGCCTAA
TTCACGTATTAAAGAAGTGTTACAGACAGGGCAAGTTCAGTTACATGAAGAGCAAAATGTTTATGGAGTTACGATTGTTA
CAAATAGGGTTCCTCTATATGTAGATGGTGAAATTGTTGGTGCGATTGCAACCTTTCGTGACAAAACAGAAATAAGAAAA
TTGGCAGAAGAAATAACAGGGATTCGATTATATGCTGAAGCATTGCGAGCGCAGTCTCATGAATTTATGAATAAGATGCA
TGTTGTACTGGGGCTTACACATATGAAGCAATATGATGAGTTACAGAAATATGTTAGTAGTATGGTATCAGAGCATCAAT
ATGAAATTGGTGGTGTTATGAAAAAAGTTAAAAATCCTGTATTTGTTGGTTTTTTACTTGGTAAACTTAGCTATGCAAGA
GAGAGGAATATAGAGCTCATTGTAAGTGAGGATTCTTATTTACCAGATATATTTGATGAAAGTATGATTCATGAGCTAAT
CACGATTGTGGGAAATTTAATTGATAATGCATTGGATGCGGTAACAAATTGTGAAGAGAAGCGAGTTCAAATTGCAATTT
ATTATGAAGACACATTAATGATTGTAGTGAAAGATACAGGACATGGGATACCGACAGATCGACTTGATACAGTGTTGATG
AAAGGATATTCTACAAAAGGAAAGAATCGAGGCTATGGGTTATTTCTTGTAAATGAAAGCATGAAGAGAATCAATGGGAA
AATACATATTCAATCATTATTAGAAAAGGGAACAACTATCACAATTGAAATACCATATACAAAGTAG

Upstream 100 bases:

>100_bases
GAATAGTATGAAATAAGTAAGGTGAAATTGTTATTGCCTTACTTATTTTTCTATCTCAAGAAGTATGATTTTGATAAACA
ATAGGGGAGTCAATGAAAGG

Downstream 100 bases:

>100_bases
GGATGAGATACAAAGATGATTAAAGTTTTAATTGTAGAAGATGATCCGATGGTAGCAATGCTAAATAAATATTATTTAGA
GCAGATTGGGGGATTCGAAC

Product: sensory histidine kinase DcuS

Products: NA

Alternate protein names: Malate kinase sensor [H]

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MRNRRLLSLWKTITLLVCTVVVLSLLVTDILISHHVERTTEESQAEKAKIIARIVAHSPIVLEGLTGRRDAVEIQTYTNR
LLKNTDVQFIVVMDMKGIRKSHPNPQKIGHHFVGGDEGTVLKGKEHVSLAEGTLGISMRVFVPVFSEAGKQVGAVAVGIS
ADNVKERTKESRHIIYIGISIGILVGIIGAVLLARHTKKILFGLEPHEIAKILEERNTMLQSVKEGIIAVDKEARVTLIN
NEAKRLLKKSSLEEEWIGKDVESYMPNSRIKEVLQTGQVQLHEEQNVYGVTIVTNRVPLYVDGEIVGAIATFRDKTEIRK
LAEEITGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYDELQKYVSSMVSEHQYEIGGVMKKVKNPVFVGFLLGKLSYAR
ERNIELIVSEDSYLPDIFDESMIHELITIVGNLIDNALDAVTNCEEKRVQIAIYYEDTLMIVVKDTGHGIPTDRLDTVLM
KGYSTKGKNRGYGLFLVNESMKRINGKIHIQSLLEKGTTITIEIPYTK

Sequences:

>Translated_528_residues
MRNRRLLSLWKTITLLVCTVVVLSLLVTDILISHHVERTTEESQAEKAKIIARIVAHSPIVLEGLTGRRDAVEIQTYTNR
LLKNTDVQFIVVMDMKGIRKSHPNPQKIGHHFVGGDEGTVLKGKEHVSLAEGTLGISMRVFVPVFSEAGKQVGAVAVGIS
ADNVKERTKESRHIIYIGISIGILVGIIGAVLLARHTKKILFGLEPHEIAKILEERNTMLQSVKEGIIAVDKEARVTLIN
NEAKRLLKKSSLEEEWIGKDVESYMPNSRIKEVLQTGQVQLHEEQNVYGVTIVTNRVPLYVDGEIVGAIATFRDKTEIRK
LAEEITGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYDELQKYVSSMVSEHQYEIGGVMKKVKNPVFVGFLLGKLSYAR
ERNIELIVSEDSYLPDIFDESMIHELITIVGNLIDNALDAVTNCEEKRVQIAIYYEDTLMIVVKDTGHGIPTDRLDTVLM
KGYSTKGKNRGYGLFLVNESMKRINGKIHIQSLLEKGTTITIEIPYTK
>Mature_528_residues
MRNRRLLSLWKTITLLVCTVVVLSLLVTDILISHHVERTTEESQAEKAKIIARIVAHSPIVLEGLTGRRDAVEIQTYTNR
LLKNTDVQFIVVMDMKGIRKSHPNPQKIGHHFVGGDEGTVLKGKEHVSLAEGTLGISMRVFVPVFSEAGKQVGAVAVGIS
ADNVKERTKESRHIIYIGISIGILVGIIGAVLLARHTKKILFGLEPHEIAKILEERNTMLQSVKEGIIAVDKEARVTLIN
NEAKRLLKKSSLEEEWIGKDVESYMPNSRIKEVLQTGQVQLHEEQNVYGVTIVTNRVPLYVDGEIVGAIATFRDKTEIRK
LAEEITGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYDELQKYVSSMVSEHQYEIGGVMKKVKNPVFVGFLLGKLSYAR
ERNIELIVSEDSYLPDIFDESMIHELITIVGNLIDNALDAVTNCEEKRVQIAIYYEDTLMIVVKDTGHGIPTDRLDTVLM
KGYSTKGKNRGYGLFLVNESMKRINGKIHIQSLLEKGTTITIEIPYTK

Specific function: Member of a two-component regulatory system malK/malR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates malR by phosphorylation [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=528, Percent_Identity=39.3939393939394, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1786837, Length=492, Percent_Identity=31.3008130081301, Blast_Score=238, Evalue=7e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59278; Mature: 59278

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNRRLLSLWKTITLLVCTVVVLSLLVTDILISHHVERTTEESQAEKAKIIARIVAHSPI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VLEGLTGRRDAVEIQTYTNRLLKNTDVQFIVVMDMKGIRKSHPNPQKIGHHFVGGDEGTV
EEECCCCCCCEEEHHHHHHHHHCCCCCEEEEEEECCHHHHCCCCHHHHCCEECCCCCCCE
LKGKEHVSLAEGTLGISMRVFVPVFSEAGKQVGAVAVGISADNVKERTKESRHIIYIGIS
EECCCCCEEECCCCCEEEHHHHHHHHHCCCHHCEEEEECCCHHHHHHHCCCCEEEEEEHH
IGILVGIIGAVLLARHTKKILFGLEPHEIAKILEERNTMLQSVKEGIIAVDKEARVTLIN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEC
NEAKRLLKKSSLEEEWIGKDVESYMPNSRIKEVLQTGQVQLHEEQNVYGVTIVTNRVPLY
HHHHHHHHHCCCCHHHHCCCHHHHCCCHHHHHHHHCCCEEEECCCCEEEEEEEECCCEEE
VDGEIVGAIATFRDKTEIRKLAEEITGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYDE
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQKYVSSMVSEHQYEIGGVMKKVKNPVFVGFLLGKLSYARERNIELIVSEDSYLPDIFDE
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
SMIHELITIVGNLIDNALDAVTNCEEKRVQIAIYYEDTLMIVVKDTGHGIPTDRLDTVLM
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECEEEEEEECCCCCCCHHHHHHHHH
KGYSTKGKNRGYGLFLVNESMKRINGKIHIQSLLEKGTTITIEIPYTK
CCCCCCCCCCCEEEEEECCHHHHCCCEEEEHHHHHCCCEEEEEECCCC
>Mature Secondary Structure
MRNRRLLSLWKTITLLVCTVVVLSLLVTDILISHHVERTTEESQAEKAKIIARIVAHSPI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VLEGLTGRRDAVEIQTYTNRLLKNTDVQFIVVMDMKGIRKSHPNPQKIGHHFVGGDEGTV
EEECCCCCCCEEEHHHHHHHHHCCCCCEEEEEEECCHHHHCCCCHHHHCCEECCCCCCCE
LKGKEHVSLAEGTLGISMRVFVPVFSEAGKQVGAVAVGISADNVKERTKESRHIIYIGIS
EECCCCCEEECCCCCEEEHHHHHHHHHCCCHHCEEEEECCCHHHHHHHCCCCEEEEEEHH
IGILVGIIGAVLLARHTKKILFGLEPHEIAKILEERNTMLQSVKEGIIAVDKEARVTLIN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEC
NEAKRLLKKSSLEEEWIGKDVESYMPNSRIKEVLQTGQVQLHEEQNVYGVTIVTNRVPLY
HHHHHHHHHCCCCHHHHCCCHHHHCCCHHHHHHHHCCCEEEECCCCEEEEEEEECCCEEE
VDGEIVGAIATFRDKTEIRKLAEEITGIRLYAEALRAQSHEFMNKMHVVLGLTHMKQYDE
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQKYVSSMVSEHQYEIGGVMKKVKNPVFVGFLLGKLSYARERNIELIVSEDSYLPDIFDE
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
SMIHELITIVGNLIDNALDAVTNCEEKRVQIAIYYEDTLMIVVKDTGHGIPTDRLDTVLM
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECEEEEEEECCCCCCCHHHHHHHHH
KGYSTKGKNRGYGLFLVNESMKRINGKIHIQSLLEKGTTITIEIPYTK
CCCCCCCCCCCEEEEEECCHHHHCCCEEEEHHHHHCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160 [H]