| Definition | Bacillus cereus subsp. cytotoxis NVH 391-98, complete genome. |
|---|---|
| Accession | NC_009674 |
| Length | 4,087,024 |
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The map label for this gene is colA [H]
Identifier: 152974319
GI number: 152974319
Start: 548387
End: 551290
Strand: Direct
Name: colA [H]
Synonym: Bcer98_0486
Alternate gene names: 152974319
Gene position: 548387-551290 (Clockwise)
Preceding gene: 152974316
Following gene: 152974320
Centisome position: 13.42
GC content: 33.47
Gene sequence:
>2904_bases ATGAATAAGAAATCAAAAATTACGCAAATGATGCTCGGTATTAGTACAGTAGCTTTATCATTTGGAAGTATTCAAACACA AGTATCAGCAGAAGAAAAAATACCTTATAATGTCATGCAAATGAAGCCGATTGGAGTTGAAACTCAGACGGATCAAATTG CTCATGTTTCGCAGGAAAATAAAACATTATCTTTTGAAGAACGATTAAGAACGGGAGATTTTTCTCAACGTCCAGCTTTT GGAATGAAGAGAACGGAATCTAAACAGTTTCAAGAAAGTTATTCTATGGCTGATTTCAATAAAATGAATAATGAAGAATT GATTGATACATTGGCAAATATTCGTTGGAATCAAATCACAGATTTATTTCAATTTAATCAAGACACAAAAACGTTTTATC AAAATAAGGAAAGAATGCAAGTTCTGATTGATGAATTAGGTCGTCGTGGGAGTACATTTACAAAAGATGATGCAAAAGGA ATTGATACATTTGTTGAAGTGTTACGTTCAGCTTTTTACCTTGGTTTCTATCATAAAGAATTAAACTATTTAAATGAGCG TAGTTTCCATGATAAATGCTTGCCAGCATTAAAAGCCATTGCGAAAAATCCAAACTTTAAACTTGGGACAGAACAACAAG ATACGGTAGTATCTGCATACGGGAAATTAATTAGTAACGCTTCGAGCGATGTTGAAACAGTGCAATATGCAGCAAATATT TTAAAACAGTACAATGATAATCTCGCTACTTATGTAAGCGATTTTATGAAAGGGCAAGCTGTATATAGTCTTATACAAGG GATTGATTATGATATAGATTCTTATGTGTATGCTTCGTATAAAGAAGCAAATCAAACAATGTGGTACGGTAAAATGGATG CTTTTGTAAATGAAGTGAGCCGTATTGCGCTTTTACAGCATGTAACAACAGAAAATAGTTGGTTAATTAATAATGGCATT TATTTTGCAGGTCGTTTAGGGAAATTTCATAGTGACCCAAATAAAGGATTAGCAGTTGTTACACAAGCAATGCATATGTA TCCGCATTTAAGTGATGCTTATTTTGTTGCAGTAGAGCAGATTAAAACGAATTATGGCGGAAAAGATTACAATGGAAATA AAATAGATGCAGAGAAAATACGTGAAGAGGGAAAACAACGGTACTTGCCAAAAACATATACATTTGATGATGGATCTATT GTATTTAAAACAGGGGATAAAGTAACAGAAGAAAAAGTGAAGCGATTGTACTGGGCTGCTAAAGAAGTAAAAGCGCAATA TCATCGTGTGATTGGAAATGATAAAGCGCTAGAGCCAGGTAATCCGGATGATGTGCTAACAGTTGTTATTTATAATAGTC CAGATGAATATAAGCTTAATAGACAACTATATGGATATGAAACAAATAATGGTGGAATTTATATTGAACAAGACGGAACT TTCTTTACATATGAACGTACCCCAGAACAAAGTATTTATAGTTTAGAAGAGTTATTCCGTCATGAATTTACTCATTATTT ACAAGGGAGATATGAGGTTCCTGGTTTATTTGGAGAAGGAGATATGTATCAAAATGAGAGATTAACTTGGTTTCAAGAAG GGAATGCAGAGTTCTTTGCAGGATCTACTCGTATGAACAATGTTATTCCACGTAAAAGTATAATTAGTGGATTATCATCT GATCCTGCAAATCGTTATACAGCAGAACGAACATTATTTGCAAAATATGGTTCATGGGATTTTTATAACTATTCTTTTGC ACTACAGTCCTATTTATATAATCATCGTTTTGAAATATTTGATCAAATTCAAGACTTAATTCGAGCGAATGATGTGAAGA ATTATGATGCATATCGTGAGGCTTTAAGTAAGGATGCGAATTTAAATACTGAGTACCAGGCGTATATGCAAAAATTAATT GATAATCAAGAAAGATATAACGTGCCAGAGGTATCAGATGATTATTTAGCAGAGCATGCACCAAAGGCATTAACAGAAGT GAAGAAAGATATCGAGGATACAACGAATCTGAAAGGGGCAACGATTAAAAAGCATAAGTCCCAATTTTTTGATACATTTA CGGTAGAAGGAACGTATACAGGTAGTACGACAAAGGGAGAAGCTGAGGACTGGAAAGAGATGAGTAAGCACATCAACCAA TCTCTAGAAAAATTGACTCAGAAGGAATGGAGCGGTTATAAAACAGTTACAGCTTATTTTGTGAACTATCGTGTAAATGC AGAAAATCAATTCGAATACGATGTTGTATTTCATGGTATCGCAACAGATAGTGGAGAAAATCAAGCTCCAGTTGTAAATA TAAATGGTCCATACAATGGAAATGTAAATCAAGCCATTCAGTTTAATAGTGATGGTTCCAAAGATGAGGATGGAAAAATT ACTTCTTACTTATGGGATTTTGGAGATGGTACAACAACTACGGAAGCAAATCCAAAACATGTATATAAGCAGGAAGGAAC ATATAAAGTAACATTAACAGTAAAAGACGATAAAGGAAAAGAAGCGAAAACCGAAACAACCGTTACTGTTAGAAAAGGAC ACGAAACAACGGTAGAATCCGAACCAAATAATCGTCTGGAGGAAGCGAATCCTATTGCATTCCATACATTGATAAAAGGT AGTCTTATGAATGAAGATCGTACAGATATTTATACTTTTGATGTCACTTCTTCAAAAAATATAGATATTTCTGTTGTAAA TGAACATAACATTGGGATGACATGGGTTCTTCATCATGAATCAGATATGCAAAATTATGTTGCGTATGGCCAAGCTAATG GAAATGATATAAAAGGGAAATTTGAAGCAAAACCAGGAAAGTATTATTTATATGTATATAAATTTGATAACGGAAATGGG ACATACACATTATCAGTAAAATAA
Upstream 100 bases:
>100_bases AACAATAATATGAAATATTGCATTTTACATTATTTTACTAGTATAATATGTTAAATTTTAAGTAATTAATATAGAAATAT AAAGATGAAAGGAAGTTATT
Downstream 100 bases:
>100_bases GTGTATGTCCGATGTATACGAAACTTTACTTTTTATGGGAAATGATATATTATATAAATATACAATATATCGGTTGTTTT TTAGGAGGAGCCTGTCACGA
Product: collagenase
Products: NA
Alternate protein names: 120 kDa collagenase [H]
Number of amino acids: Translated: 967; Mature: 967
Protein sequence:
>967_residues MNKKSKITQMMLGISTVALSFGSIQTQVSAEEKIPYNVMQMKPIGVETQTDQIAHVSQENKTLSFEERLRTGDFSQRPAF GMKRTESKQFQESYSMADFNKMNNEELIDTLANIRWNQITDLFQFNQDTKTFYQNKERMQVLIDELGRRGSTFTKDDAKG IDTFVEVLRSAFYLGFYHKELNYLNERSFHDKCLPALKAIAKNPNFKLGTEQQDTVVSAYGKLISNASSDVETVQYAANI LKQYNDNLATYVSDFMKGQAVYSLIQGIDYDIDSYVYASYKEANQTMWYGKMDAFVNEVSRIALLQHVTTENSWLINNGI YFAGRLGKFHSDPNKGLAVVTQAMHMYPHLSDAYFVAVEQIKTNYGGKDYNGNKIDAEKIREEGKQRYLPKTYTFDDGSI VFKTGDKVTEEKVKRLYWAAKEVKAQYHRVIGNDKALEPGNPDDVLTVVIYNSPDEYKLNRQLYGYETNNGGIYIEQDGT FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGEGDMYQNERLTWFQEGNAEFFAGSTRMNNVIPRKSIISGLSS DPANRYTAERTLFAKYGSWDFYNYSFALQSYLYNHRFEIFDQIQDLIRANDVKNYDAYREALSKDANLNTEYQAYMQKLI DNQERYNVPEVSDDYLAEHAPKALTEVKKDIEDTTNLKGATIKKHKSQFFDTFTVEGTYTGSTTKGEAEDWKEMSKHINQ SLEKLTQKEWSGYKTVTAYFVNYRVNAENQFEYDVVFHGIATDSGENQAPVVNINGPYNGNVNQAIQFNSDGSKDEDGKI TSYLWDFGDGTTTTEANPKHVYKQEGTYKVTLTVKDDKGKEAKTETTVTVRKGHETTVESEPNNRLEEANPIAFHTLIKG SLMNEDRTDIYTFDVTSSKNIDISVVNEHNIGMTWVLHHESDMQNYVAYGQANGNDIKGKFEAKPGKYYLYVYKFDNGNG TYTLSVK
Sequences:
>Translated_967_residues MNKKSKITQMMLGISTVALSFGSIQTQVSAEEKIPYNVMQMKPIGVETQTDQIAHVSQENKTLSFEERLRTGDFSQRPAF GMKRTESKQFQESYSMADFNKMNNEELIDTLANIRWNQITDLFQFNQDTKTFYQNKERMQVLIDELGRRGSTFTKDDAKG IDTFVEVLRSAFYLGFYHKELNYLNERSFHDKCLPALKAIAKNPNFKLGTEQQDTVVSAYGKLISNASSDVETVQYAANI LKQYNDNLATYVSDFMKGQAVYSLIQGIDYDIDSYVYASYKEANQTMWYGKMDAFVNEVSRIALLQHVTTENSWLINNGI YFAGRLGKFHSDPNKGLAVVTQAMHMYPHLSDAYFVAVEQIKTNYGGKDYNGNKIDAEKIREEGKQRYLPKTYTFDDGSI VFKTGDKVTEEKVKRLYWAAKEVKAQYHRVIGNDKALEPGNPDDVLTVVIYNSPDEYKLNRQLYGYETNNGGIYIEQDGT FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGEGDMYQNERLTWFQEGNAEFFAGSTRMNNVIPRKSIISGLSS DPANRYTAERTLFAKYGSWDFYNYSFALQSYLYNHRFEIFDQIQDLIRANDVKNYDAYREALSKDANLNTEYQAYMQKLI DNQERYNVPEVSDDYLAEHAPKALTEVKKDIEDTTNLKGATIKKHKSQFFDTFTVEGTYTGSTTKGEAEDWKEMSKHINQ SLEKLTQKEWSGYKTVTAYFVNYRVNAENQFEYDVVFHGIATDSGENQAPVVNINGPYNGNVNQAIQFNSDGSKDEDGKI TSYLWDFGDGTTTTEANPKHVYKQEGTYKVTLTVKDDKGKEAKTETTVTVRKGHETTVESEPNNRLEEANPIAFHTLIKG SLMNEDRTDIYTFDVTSSKNIDISVVNEHNIGMTWVLHHESDMQNYVAYGQANGNDIKGKFEAKPGKYYLYVYKFDNGNG TYTLSVK >Mature_967_residues MNKKSKITQMMLGISTVALSFGSIQTQVSAEEKIPYNVMQMKPIGVETQTDQIAHVSQENKTLSFEERLRTGDFSQRPAF GMKRTESKQFQESYSMADFNKMNNEELIDTLANIRWNQITDLFQFNQDTKTFYQNKERMQVLIDELGRRGSTFTKDDAKG IDTFVEVLRSAFYLGFYHKELNYLNERSFHDKCLPALKAIAKNPNFKLGTEQQDTVVSAYGKLISNASSDVETVQYAANI LKQYNDNLATYVSDFMKGQAVYSLIQGIDYDIDSYVYASYKEANQTMWYGKMDAFVNEVSRIALLQHVTTENSWLINNGI YFAGRLGKFHSDPNKGLAVVTQAMHMYPHLSDAYFVAVEQIKTNYGGKDYNGNKIDAEKIREEGKQRYLPKTYTFDDGSI VFKTGDKVTEEKVKRLYWAAKEVKAQYHRVIGNDKALEPGNPDDVLTVVIYNSPDEYKLNRQLYGYETNNGGIYIEQDGT FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGEGDMYQNERLTWFQEGNAEFFAGSTRMNNVIPRKSIISGLSS DPANRYTAERTLFAKYGSWDFYNYSFALQSYLYNHRFEIFDQIQDLIRANDVKNYDAYREALSKDANLNTEYQAYMQKLI DNQERYNVPEVSDDYLAEHAPKALTEVKKDIEDTTNLKGATIKKHKSQFFDTFTVEGTYTGSTTKGEAEDWKEMSKHINQ SLEKLTQKEWSGYKTVTAYFVNYRVNAENQFEYDVVFHGIATDSGENQAPVVNINGPYNGNVNQAIQFNSDGSKDEDGKI TSYLWDFGDGTTTTEANPKHVYKQEGTYKVTLTVKDDKGKEAKTETTVTVRKGHETTVESEPNNRLEEANPIAFHTLIKG SLMNEDRTDIYTFDVTSSKNIDISVVNEHNIGMTWVLHHESDMQNYVAYGQANGNDIKGKFEAKPGKYYLYVYKFDNGNG TYTLSVK
Specific function: Unknown
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 PKD domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013510 - InterPro: IPR007280 - InterPro: IPR002169 - InterPro: IPR013661 - InterPro: IPR022409 - InterPro: IPR000601 [H]
Pfam domain/function: PF01752 Peptidase_M9; PF08453 Peptidase_M9_N; PF00801 PKD; PF04151 PPC [H]
EC number: =3.4.24.3 [H]
Molecular weight: Translated: 110981; Mature: 110981
Theoretical pI: Translated: 5.27; Mature: 5.27
Prosite motif: PS50093 PKD ; PS00142 ZINC_PROTEASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 2.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNKKSKITQMMLGISTVALSFGSIQTQVSAEEKIPYNVMQMKPIGVETQTDQIAHVSQEN CCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCC KTLSFEERLRTGDFSQRPAFGMKRTESKQFQESYSMADFNKMNNEELIDTLANIRWNQIT CCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHCCHHHHHHHHHHCCHHHHH DLFQFNQDTKTFYQNKERMQVLIDELGRRGSTFTKDDAKGIDTFVEVLRSAFYLGFYHKE HHHHCCCCHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH LNYLNERSFHDKCLPALKAIAKNPNFKLGTEQQDTVVSAYGKLISNASSDVETVQYAANI HHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH LKQYNDNLATYVSDFMKGQAVYSLIQGIDYDIDSYVYASYKEANQTMWYGKMDAFVNEVS HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEHHHCCCEEEEEHHHHHHHHHH RIALLQHVTTENSWLINNGIYFAGRLGKFHSDPNKGLAVVTQAMHMYPHLSDAYFVAVEQ HHHHHHHHCCCCCEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHEEEHHH IKTNYGGKDYNGNKIDAEKIREEGKQRYLPKTYTFDDGSIVFKTGDKVTEEKVKRLYWAA HHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHH KEVKAQYHRVIGNDKALEPGNPDDVLTVVIYNSPDEYKLNRQLYGYETNNGGIYIEQDGT HHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEECCEEEEEEECCCCEEEEECCC FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGEGDMYQNERLTWFQEGNAEFFA EEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEE GSTRMNNVIPRKSIISGLSSDPANRYTAERTLFAKYGSWDFYNYSFALQSYLYNHRFEIF CCCHHCCCCCHHHHHHCCCCCCCCHHHHHHEEEEECCCCCEEEHHHHHHHHHHHHHHHHH DQIQDLIRANDVKNYDAYREALSKDANLNTEYQAYMQKLIDNQERYNVPEVSDDYLAEHA HHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHH PKALTEVKKDIEDTTNLKGATIKKHKSQFFDTFTVEGTYTGSTTKGEAEDWKEMSKHINQ HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCEEEEEEEEECCCCCCCCHHHHHHHHHHHHH SLEKLTQKEWSGYKTVTAYFVNYRVNAENQFEYDVVFHGIATDSGENQAPVVNINGPYNG HHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCEEECCCCCCC NVNQAIQFNSDGSKDEDGKITSYLWDFGDGTTTTEANPKHVYKQEGTYKVTLTVKDDKGK CCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCCEEEEEEEECCCCC EAKTETTVTVRKGHETTVESEPNNRLEEANPIAFHTLIKGSLMNEDRTDIYTFDVTSSKN CCCCCEEEEEECCCCCCCCCCCCCCHHCCCCEEEEHHHHHHCCCCCCCEEEEEEECCCCC IDISVVNEHNIGMTWVLHHESDMQNYVAYGQANGNDIKGKFEAKPGKYYLYVYKFDNGNG EEEEEEECCCCCEEEEEECCCCCCHHHEECCCCCCCCCEEEECCCCEEEEEEEEEECCCE TYTLSVK EEEEEEC >Mature Secondary Structure MNKKSKITQMMLGISTVALSFGSIQTQVSAEEKIPYNVMQMKPIGVETQTDQIAHVSQEN CCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCC KTLSFEERLRTGDFSQRPAFGMKRTESKQFQESYSMADFNKMNNEELIDTLANIRWNQIT CCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHCCHHHHHHHHHHCCHHHHH DLFQFNQDTKTFYQNKERMQVLIDELGRRGSTFTKDDAKGIDTFVEVLRSAFYLGFYHKE HHHHCCCCHHHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH LNYLNERSFHDKCLPALKAIAKNPNFKLGTEQQDTVVSAYGKLISNASSDVETVQYAANI HHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH LKQYNDNLATYVSDFMKGQAVYSLIQGIDYDIDSYVYASYKEANQTMWYGKMDAFVNEVS HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEHHHCCCEEEEEHHHHHHHHHH RIALLQHVTTENSWLINNGIYFAGRLGKFHSDPNKGLAVVTQAMHMYPHLSDAYFVAVEQ HHHHHHHHCCCCCEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHEEEHHH IKTNYGGKDYNGNKIDAEKIREEGKQRYLPKTYTFDDGSIVFKTGDKVTEEKVKRLYWAA HHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHH KEVKAQYHRVIGNDKALEPGNPDDVLTVVIYNSPDEYKLNRQLYGYETNNGGIYIEQDGT HHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEECCEEEEEEECCCCEEEEECCC FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGEGDMYQNERLTWFQEGNAEFFA EEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEE GSTRMNNVIPRKSIISGLSSDPANRYTAERTLFAKYGSWDFYNYSFALQSYLYNHRFEIF CCCHHCCCCCHHHHHHCCCCCCCCHHHHHHEEEEECCCCCEEEHHHHHHHHHHHHHHHHH DQIQDLIRANDVKNYDAYREALSKDANLNTEYQAYMQKLIDNQERYNVPEVSDDYLAEHA HHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHH PKALTEVKKDIEDTTNLKGATIKKHKSQFFDTFTVEGTYTGSTTKGEAEDWKEMSKHINQ HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCEEEEEEEEECCCCCCCCHHHHHHHHHHHHH SLEKLTQKEWSGYKTVTAYFVNYRVNAENQFEYDVVFHGIATDSGENQAPVVNINGPYNG HHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCCEEECCCCCCC NVNQAIQFNSDGSKDEDGKITSYLWDFGDGTTTTEANPKHVYKQEGTYKVTLTVKDDKGK CCCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCCEEEEEEEECCCCC EAKTETTVTVRKGHETTVESEPNNRLEEANPIAFHTLIKGSLMNEDRTDIYTFDVTSSKN CCCCCEEEEEECCCCCCCCCCCCCCHHCCCCEEEEHHHHHHCCCCCCCEEEEEEECCCCC IDISVVNEHNIGMTWVLHHESDMQNYVAYGQANGNDIKGKFEAKPGKYYLYVYKFDNGNG EEEEEEECCCCCEEEEEECCCCCCHHHEECCCCCCCCCEEEECCCCEEEEEEEEEECCCE TYTLSVK EEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8282691; 11792842 [H]