Click here to switch to the map view.

The map label for this gene is pflA [H]

Identifier: 152974265

GI number: 152974265

Start: 488891

End: 489622

Strand: Direct

Name: pflA [H]

Synonym: Bcer98_0432

Alternate gene names: 152974265

Gene position: 488891-489622 (Clockwise)

Preceding gene: 152974264

Following gene: 152974266

Centisome position: 11.96

GC content: 33.74

Gene sequence:

>732_bases
ATGGTAAAAGGAAGAATTCATTCTGTAGAGTCTTGTGGTACTGTTGATGGCCCAGGAATTCGTTATGTCATATTTACACA
AGGGTGTTTATTACGTTGCCAATATTGTCATAATGCTGATACATGGGAGATTGGTAAAGGAAAAGAAATAACAGTTGATG
AAATTATGCAGGATGTTACATGTTACCTTCCCTTTATTGAGGCTTCAGAAGGCGGTATTACAGTTAGTGGTGGAGAACCA
TTATTACAATTAGATTTCCTAATTGAATTATTCAAAAAATGTAAGGAAGTTGGAATTCATACAACAATCGATTCTTCGGG
AGGGTGTTACTCAGAAGAACCAGAATTCCAAAGAAAATTAGATCTCTTAATGGATTACACAGATTTGGTTTTACTGGACT
TAAAACATATTGATTCCAAAAAGCATCGAAAATTAACAGGAAAACCAAATGAACATATTTTACAATTTGCTCGTTATTTA
TCAGATAAAAAGAAATCCATTTGGGTAAGACATGTATTAGTTCCTGGCGTTACAGATAGCGAGAAAGATTTACAAAGATT
ATCTCAGTTTATTCAAAGTTTGTCTAATGTACAAAAGATTGAAGTGCTACCATATCACAAACTTGGTGTATATAAGTGGG
AAACACTTGGACATAAGTATCCCCTTCAAGGTGTTGAACCTCCAACAGAACAAAGTGTTTTACATGCAAAAGAAATTTTA
AAAGCGGTCTAA

Upstream 100 bases:

>100_bases
GTAATTAATCGTACAATGCATGAAAGCATGTAAAGAAAAATTTCCCCCTATCTCTTAATAAGGGCACGGGGGAAATTGTT
TCAATAAAGGAGGAAGTAAC

Downstream 100 bases:

>100_bases
TCCATAGTTTGGATTAGACTTTTCTTTTTCCTCTAATAAACGCTAGAGTATATATAGATAAAGGAGTATACTGAAAGTAG
AATATCCATATTTTTGTAAA

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVDEIMQDVTCYLPFIEASEGGITVSGGEP
LLQLDFLIELFKKCKEVGIHTTIDSSGGCYSEEPEFQRKLDLLMDYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYL
SDKKKSIWVRHVLVPGVTDSEKDLQRLSQFIQSLSNVQKIEVLPYHKLGVYKWETLGHKYPLQGVEPPTEQSVLHAKEIL
KAV

Sequences:

>Translated_243_residues
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVDEIMQDVTCYLPFIEASEGGITVSGGEP
LLQLDFLIELFKKCKEVGIHTTIDSSGGCYSEEPEFQRKLDLLMDYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYL
SDKKKSIWVRHVLVPGVTDSEKDLQRLSQFIQSLSNVQKIEVLPYHKLGVYKWETLGHKYPLQGVEPPTEQSVLHAKEIL
KAV
>Mature_243_residues
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVDEIMQDVTCYLPFIEASEGGITVSGGEP
LLQLDFLIELFKKCKEVGIHTTIDSSGGCYSEEPEFQRKLDLLMDYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYL
SDKKKSIWVRHVLVPGVTDSEKDLQRLSQFIQSLSNVQKIEVLPYHKLGVYKWETLGHKYPLQGVEPPTEQSVLHAKEIL
KAV

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=240, Percent_Identity=48.3333333333333, Blast_Score=258, Evalue=2e-70,
Organism=Escherichia coli, GI1790389, Length=266, Percent_Identity=26.6917293233083, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1790839, Length=247, Percent_Identity=29.1497975708502, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI226510931, Length=273, Percent_Identity=26.007326007326, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27583; Mature: 27583

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVDEIMQDVT
CCCCCCCCHHCCCCCCCCCEEEEEEECCHHHEEHHHCCCCCEECCCCCEECHHHHHHHHH
CYLPFIEASEGGITVSGGEPLLQLDFLIELFKKCKEVGIHTTIDSSGGCYSEEPEFQRKL
HHHCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHH
DLLMDYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYLSDKKKSIWVRHVLVPGVTDS
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHCHHHHEEEEEEECCCCCCC
EKDLQRLSQFIQSLSNVQKIEVLPYHKLGVYKWETLGHKYPLQGVEPPTEQSVLHAKEIL
HHHHHHHHHHHHHHHCCCEEEECCCHHCCCEEHHHCCCCCCCCCCCCCHHHHHHHHHHHH
KAV
HCC
>Mature Secondary Structure
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVDEIMQDVT
CCCCCCCCHHCCCCCCCCCEEEEEEECCHHHEEHHHCCCCCEECCCCCEECHHHHHHHHH
CYLPFIEASEGGITVSGGEPLLQLDFLIELFKKCKEVGIHTTIDSSGGCYSEEPEFQRKL
HHHCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHH
DLLMDYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYLSDKKKSIWVRHVLVPGVTDS
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHCHHHHEEEEEEECCCCCCC
EKDLQRLSQFIQSLSNVQKIEVLPYHKLGVYKWETLGHKYPLQGVEPPTEQSVLHAKEIL
HHHHHHHHHHHHHHHCCCEEEECCCHHCCCEEHHHCCCCCCCCCCCCCHHHHHHHHHHHH
KAV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]