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The map label for this gene is truA2 [H]

Identifier: 152974254

GI number: 152974254

Start: 476107

End: 476889

Strand: Direct

Name: truA2 [H]

Synonym: Bcer98_0421

Alternate gene names: 152974254

Gene position: 476107-476889 (Clockwise)

Preceding gene: 152974253

Following gene: 152974256

Centisome position: 11.65

GC content: 35.63

Gene sequence:

>783_bases
ATGTGGAGAATGTGGTTAGGCTTGTTAGAAAATAAGGAGAATAAAATGAACAACTACAAACTCACAATTCAATATGACGG
CGGACGCTTTAAAGGCTGGCAGCGTCTTGGTAATAACGATAATACAATCCAGGGAAAAATTGAAAGTGTACTGTCAGAAA
TGATTGGACAAGAAATTGAAATTATCGGCTGTAGCAGAACAGATGCTGGGGTACACGCTCTAAACCAAGTAGCGAACTTT
AAGGTCGATGAAAAATTAGTGGAAACGAAAGTGAAAAAATATTTAAATCAATACTTACCAAACGATATTAGCATCACGGA
GGTGGAGCAGGTTCCTGAACGTTTTCACGCTCGCTACAACTCAAAGACAAAAACATATTTATATAAAATTTGGAATGAGG
AACATACGAATCCATTTATGCGTAAATACAGTATGCATGTTCCTAAAAAGTTAAATGTTGAAAACATGAAACAAGCAGCT
CACTATTTCATTGGTTCACATGATTTTACTGCTTTTTCAAATGCGAAATCAAAAAAGAAATCAATGGTTCGGGAAATATA
CGTGCTCGATGTTTTTGAAAGCGAAGGGTTTATTCAAATTCGAGTGAGCGGAAATGGTTTTCTTCACAATATGGTAAGAA
AAATTGTTGGAGCATTAATTGAAGTCGGATTAGGACAGCTTGATGCTGAAGCAATTCCGCAAATTTTAGAAGCAAAACAA
AGGAATCAGATTAATTGCCTTGCGGATGCGAATGGATTGTATTTAGAAAAGGTTGATTTTTAA

Upstream 100 bases:

>100_bases
ATACGAAGAAGTCAAAAGACGTAGACATTACGAATCCAGATTTGTGTTCACAAGCAAGTGACGAGGATAGTAAAATGTGT
TCACTTGCTAGTGACCACAC

Downstream 100 bases:

>100_bases
ATGGTGAAAGAGACTGTAAAATTGTAAACAGTCTCTTTTTTTGCAGGCTTGTTTATAGAGGTAAGGTGACGAATTTTGTT
GTCTGGTCGATATAGCATGG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 2; tRNA-uridine isomerase I 2 [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MWRMWLGLLENKENKMNNYKLTIQYDGGRFKGWQRLGNNDNTIQGKIESVLSEMIGQEIEIIGCSRTDAGVHALNQVANF
KVDEKLVETKVKKYLNQYLPNDISITEVEQVPERFHARYNSKTKTYLYKIWNEEHTNPFMRKYSMHVPKKLNVENMKQAA
HYFIGSHDFTAFSNAKSKKKSMVREIYVLDVFESEGFIQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPQILEAKQ
RNQINCLADANGLYLEKVDF

Sequences:

>Translated_260_residues
MWRMWLGLLENKENKMNNYKLTIQYDGGRFKGWQRLGNNDNTIQGKIESVLSEMIGQEIEIIGCSRTDAGVHALNQVANF
KVDEKLVETKVKKYLNQYLPNDISITEVEQVPERFHARYNSKTKTYLYKIWNEEHTNPFMRKYSMHVPKKLNVENMKQAA
HYFIGSHDFTAFSNAKSKKKSMVREIYVLDVFESEGFIQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPQILEAKQ
RNQINCLADANGLYLEKVDF
>Mature_260_residues
MWRMWLGLLENKENKMNNYKLTIQYDGGRFKGWQRLGNNDNTIQGKIESVLSEMIGQEIEIIGCSRTDAGVHALNQVANF
KVDEKLVETKVKKYLNQYLPNDISITEVEQVPERFHARYNSKTKTYLYKIWNEEHTNPFMRKYSMHVPKKLNVENMKQAA
HYFIGSHDFTAFSNAKSKKKSMVREIYVLDVFESEGFIQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPQILEAKQ
RNQINCLADANGLYLEKVDF

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=273, Percent_Identity=28.9377289377289, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI70166634, Length=262, Percent_Identity=26.3358778625954, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI70166599, Length=262, Percent_Identity=26.3358778625954, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI70166645, Length=262, Percent_Identity=26.3358778625954, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788657, Length=240, Percent_Identity=34.1666666666667, Blast_Score=156, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71985046, Length=266, Percent_Identity=27.4436090225564, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321112, Length=210, Percent_Identity=30, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI116007752, Length=303, Percent_Identity=28.0528052805281, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 30073; Mature: 30073

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRMWLGLLENKENKMNNYKLTIQYDGGRFKGWQRLGNNDNTIQGKIESVLSEMIGQEIE
CHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCEEHHHHHHHHHHHCCCCEE
IIGCSRTDAGVHALNQVANFKVDEKLVETKVKKYLNQYLPNDISITEVEQVPERFHARYN
EEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
SKTKTYLYKIWNEEHTNPFMRKYSMHVPKKLNVENMKQAAHYFIGSHDFTAFSNAKSKKK
CCHHEEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHCHHHHHH
SMVREIYVLDVFESEGFIQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPQILEAKQ
HHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RNQINCLADANGLYLEKVDF
CCCEEEEECCCCEEEEECCC
>Mature Secondary Structure
MWRMWLGLLENKENKMNNYKLTIQYDGGRFKGWQRLGNNDNTIQGKIESVLSEMIGQEIE
CHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCEEHHHHHHHHHHHCCCCEE
IIGCSRTDAGVHALNQVANFKVDEKLVETKVKKYLNQYLPNDISITEVEQVPERFHARYN
EEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
SKTKTYLYKIWNEEHTNPFMRKYSMHVPKKLNVENMKQAAHYFIGSHDFTAFSNAKSKKK
CCHHEEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHCHHHHHH
SMVREIYVLDVFESEGFIQIRVSGNGFLHNMVRKIVGALIEVGLGQLDAEAIPQILEAKQ
HHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RNQINCLADANGLYLEKVDF
CCCEEEEECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]