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The map label for this gene is proS2 [H]

Identifier: 152974217

GI number: 152974217

Start: 439934

End: 441364

Strand: Direct

Name: proS2 [H]

Synonym: Bcer98_0382

Alternate gene names: 152974217

Gene position: 439934-441364 (Clockwise)

Preceding gene: 152974216

Following gene: 152974218

Centisome position: 10.76

GC content: 36.2

Gene sequence:

>1431_bases
ATGAAAAAAGAACAAGTGCAAGCCATTACAAAGATGGAGGAAGATTTTGCTCAGTGGTATACCGATATTGTGAAGAAAGC
AGAACTGGTTGATTATTCAAGTGTAAAAGGTTGTATGATTTTACGTCCATATGGATATGCATTATGGGAGAATATACAAA
AAGTGCTGGATGAAAAATTAAAAGCATCCGGTCATGAAAATGTGTATATGCCAATGTTTATTCCAGAAAACTTACTTCAA
AAAGAAAAGGATCATGTGGAAGGCTTTGCACCTAAAGTAGCATGGGTTACACATGGAGGAGATGAGAAGTTAGGGGAAAG
ACTTTGTGTGCGTCCAACATCTGAAACATTATTTTGTGAGCATTTTTCAAAAATTGTTCAGTCTTATAATGATTTACCTA
AATTATATAATCAGTGGTGTTCAGTTGTCCGTTGGGAAAAAACGACGAGACCATTTCTTCGCACAACTGAATTTTTATGG
CAAGAAGGTCATACAATTCATGAAACGGCAGAGGAATCGCAAAGGGAAACTTTAAATATTTTGAATGTATATGCTTCATT
TTGTGAAGAGTATTTAGCAATTCCAGTCATTAAAGGGAAGAAAACGGAAAAGGAAAAATTTGCAGGAGCAAAAGCGACGT
ATACAATTGAAAGTTTAATGCACGATGGGAAAGCACTTCAAACAGGAACATCTCATAATTTTGGTACAAATTTCTCTGAA
GCATTTGAGATTAAATATTTAGATCGGAATGGAAAATGGCAATATGTACATCAAACATCTTGGGGCGTATCCACAAGAAT
GATTGGGGGGCTTATTATGGTTCATAGTGATAATAATGGACTTGTTATACCGCCAAAAGTTGCACCAGTGCAGGTGATGA
TTGTGCCAATTGCTCAGCATAAAGAAGGGATATTGGAAAAGGTAGCAGAGTTAAAAGAGCGAATTCAACAAGTTGCACGT
GTAAGAATAGATGCGAGTAGTAAAACACCAGGATGGAAGTTTAATGAGTATGAAATGAAAGGTGTGCCAATTCGTTTAGA
AGTTGGTCCAAAAGATATCGAAAAAAATCAAGTAGTACTTGTTAGACGAGATACGAAGGAGAAAGAATGTGTATCAATGG
ATCAATTAGAAGAGCGGATTGTATTGGTACTTAATGAAATTCATAATTTCTTATTTCAGAAAGCAAAGGCATTTCGTAAT
GCAAACACATACAGCGTTACAAGTTTTGATGAAATGAAGCAAGTAGCTAATGCAAAGCAAGGGTTTATTAAAGCGATGTG
GTGCGGCGACCTTACATGTGAAGAGAAATTAAAAGAAGAGGTAGGCGTGACATCGCGTTGCATACCATTTGAACAAGAGC
AGTTAGCGGAAGAATGTGTATGCTGTAACAAAAAAGCGAACAATTTAGTCTATTGGGGAAAAGCATATTAA

Upstream 100 bases:

>100_bases
GTACAACTTTTGTGCATGAATCAGGGTGGTAACGCCGGCAAAAGCTCGGTCCCTATTTCGGGACGCGGGCTTTTTTTTAT
GTTTTAAAGGAGGGGAAAAT

Downstream 100 bases:

>100_bases
TGTATGTGACTTAGTCTGGAAAAGAATCTTACGTGTAGAATGAAGATCCTTTTTTTATATGAATTTCGATTCGTTATAAT
TAAGTATAGAAAAGTAAGGG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase 2; ProRS 2 [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENIQKVLDEKLKASGHENVYMPMFIPENLLQ
KEKDHVEGFAPKVAWVTHGGDEKLGERLCVRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLW
QEGHTIHETAEESQRETLNILNVYASFCEEYLAIPVIKGKKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
AFEIKYLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVIPPKVAPVQVMIVPIAQHKEGILEKVAELKERIQQVAR
VRIDASSKTPGWKFNEYEMKGVPIRLEVGPKDIEKNQVVLVRRDTKEKECVSMDQLEERIVLVLNEIHNFLFQKAKAFRN
ANTYSVTSFDEMKQVANAKQGFIKAMWCGDLTCEEKLKEEVGVTSRCIPFEQEQLAEECVCCNKKANNLVYWGKAY

Sequences:

>Translated_476_residues
MKKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENIQKVLDEKLKASGHENVYMPMFIPENLLQ
KEKDHVEGFAPKVAWVTHGGDEKLGERLCVRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLW
QEGHTIHETAEESQRETLNILNVYASFCEEYLAIPVIKGKKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
AFEIKYLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVIPPKVAPVQVMIVPIAQHKEGILEKVAELKERIQQVAR
VRIDASSKTPGWKFNEYEMKGVPIRLEVGPKDIEKNQVVLVRRDTKEKECVSMDQLEERIVLVLNEIHNFLFQKAKAFRN
ANTYSVTSFDEMKQVANAKQGFIKAMWCGDLTCEEKLKEEVGVTSRCIPFEQEQLAEECVCCNKKANNLVYWGKAY
>Mature_476_residues
MKKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENIQKVLDEKLKASGHENVYMPMFIPENLLQ
KEKDHVEGFAPKVAWVTHGGDEKLGERLCVRPTSETLFCEHFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLW
QEGHTIHETAEESQRETLNILNVYASFCEEYLAIPVIKGKKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
AFEIKYLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVIPPKVAPVQVMIVPIAQHKEGILEKVAELKERIQQVAR
VRIDASSKTPGWKFNEYEMKGVPIRLEVGPKDIEKNQVVLVRRDTKEKECVSMDQLEERIVLVLNEIHNFLFQKAKAFRN
ANTYSVTSFDEMKQVANAKQGFIKAMWCGDLTCEEKLKEEVGVTSRCIPFEQEQLAEECVCCNKKANNLVYWGKAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=496, Percent_Identity=43.75, Blast_Score=423, Evalue=1e-118,
Organism=Escherichia coli, GI1786392, Length=161, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71991342, Length=490, Percent_Identity=40.8163265306122, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71991346, Length=357, Percent_Identity=45.6582633053221, Blast_Score=352, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6321808, Length=505, Percent_Identity=46.1386138613861, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24649468, Length=495, Percent_Identity=41.6161616161616, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24649466, Length=495, Percent_Identity=41.6161616161616, Blast_Score=396, Evalue=1e-110,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 54836; Mature: 54836

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENIQKVLDEKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
KASGHENVYMPMFIPENLLQKEKDHVEGFAPKVAWVTHGGDEKLGERLCVRPTSETLFCE
HCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCEECCCCCCHHHHH
HFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETAEESQRETLNI
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LNVYASFCEEYLAIPVIKGKKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
HHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
AFEIKYLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVIPPKVAPVQVMIVPIAQH
EEEEEEECCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEECCCCCCEEEEEEECHHH
KEGILEKVAELKERIQQVARVRIDASSKTPGWKFNEYEMKGVPIRLEVGPKDIEKNQVVL
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCEEE
VRRDTKEKECVSMDQLEERIVLVLNEIHNFLFQKAKAFRNANTYSVTSFDEMKQVANAKQ
EECCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHH
GFIKAMWCGDLTCEEKLKEEVGVTSRCIPFEQEQLAEECVCCNKKANNLVYWGKAY
HHEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCC
>Mature Secondary Structure
MKKEQVQAITKMEEDFAQWYTDIVKKAELVDYSSVKGCMILRPYGYALWENIQKVLDEKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
KASGHENVYMPMFIPENLLQKEKDHVEGFAPKVAWVTHGGDEKLGERLCVRPTSETLFCE
HCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCEECCCCCCHHHHH
HFSKIVQSYNDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETAEESQRETLNI
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LNVYASFCEEYLAIPVIKGKKTEKEKFAGAKATYTIESLMHDGKALQTGTSHNFGTNFSE
HHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
AFEIKYLDRNGKWQYVHQTSWGVSTRMIGGLIMVHSDNNGLVIPPKVAPVQVMIVPIAQH
EEEEEEECCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEECCCCCCEEEEEEECHHH
KEGILEKVAELKERIQQVARVRIDASSKTPGWKFNEYEMKGVPIRLEVGPKDIEKNQVVL
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCEEE
VRRDTKEKECVSMDQLEERIVLVLNEIHNFLFQKAKAFRNANTYSVTSFDEMKQVANAKQ
EECCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHH
GFIKAMWCGDLTCEEKLKEEVGVTSRCIPFEQEQLAEECVCCNKKANNLVYWGKAY
HHEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]