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The map label for this gene is dus2 [H]

Identifier: 152974153

GI number: 152974153

Start: 359308

End: 360267

Strand: Direct

Name: dus2 [H]

Synonym: Bcer98_0312

Alternate gene names: 152974153

Gene position: 359308-360267 (Clockwise)

Preceding gene: 152974152

Following gene: 152974154

Centisome position: 8.79

GC content: 41.35

Gene sequence:

>960_bases
ATGATAGATAATTTTTGGCGTGATTTACCACGACCATTTTTTGTACTTGCACCAATGGAAGATGTGACAGATGTTGTTTT
TCGTCACGTAGTAAGTGAAGCCGGTCGACCGGATGTATTTTTCACGGAGTTTACAAACTCGGATAGCTATTGTCATCCAG
AGGGTATGAAAAGTGTGCGTGGCCGTTTGATTTTTACAGAAGATGAACAGCCAATAGTGGCGCATATTTGGGGAGATAAT
CCCGAATATTTCCGTCAAATGAGTATTGGCATGGCAGAACTAGGGTTTAAAGGAATCGATATTAATATGGGCTGTCCTGT
ACCGAATGTAGCTTCGAGAGGAAAAGGTAGTGGCCTTATTCTGCGTCCAGACGTCGCAGCAGAACTTATTCAAGCAGCAA
AAGCAGGCGGACTACCTGTCAGCGTAAAAACACGACTTGGCTTTAAGGAAGTAAATGAGTGGGAGGAGTGGTTAACGCAT
ATTTTCAAACAGGATATTGCGAACCTTTCTATTCATTTACGTACAAGAGAAGAAATGAGCCAAGTGGCTGCTCATTGGGA
GTTAATTCCAGAAATCAAAAAATTACGTGACCGTATCGCACCAAATACGCTCCTAACAATCAATGGAGACATTCCTGACC
GTCAAACCGGGCTACAGCTTGCTGAAAAATACGGCATTGATGGTGTGATGATCGGGCGAGGTATTTTTAAAAATCCGTTT
GCGTTTGAAAAAGAGCCAAGAGAACATAGCAGTAAAGAACACCTTGATCTTTTAAGACTACAGCTTGATCTTCAAGATCA
ATATGCGGAAGTACTACCACGTTCCATCACAGGACTTCATCGCTTTTTCAAAATTTATGTTAAAGGATTCCATGGAGCTG
CTGAATTAAGAAATCAATTGATGAGCACGAAATCAACAGATGAAGTGCGTGCATTGCTTGATAAATTTGAACAGAGATGA

Upstream 100 bases:

>100_bases
TTTTTAAAAATAATAAATGTTCAAATGTACTGGTGTTTGATAATAAGCGATTGTAACATTTGACTTTAGACTTTTTTAAT
TGAGAGGATTTGAGTTTGAC

Downstream 100 bases:

>100_bases
AGGAATGAAAAAGCAGGGGCTTTCTAGCTCCTGCTTTTTGTCGTTATATGTCGCGAAATCGATATCCTTTGTCGAATAGT
CGATAAGTTGTGTCTAAGCG

Product: dihydrouridine synthase DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPIVAHIWGDN
PEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKEVNEWEEWLTH
IFKQDIANLSIHLRTREEMSQVAAHWELIPEIKKLRDRIAPNTLLTINGDIPDRQTGLQLAEKYGIDGVMIGRGIFKNPF
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFHGAAELRNQLMSTKSTDEVRALLDKFEQR

Sequences:

>Translated_319_residues
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPIVAHIWGDN
PEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKEVNEWEEWLTH
IFKQDIANLSIHLRTREEMSQVAAHWELIPEIKKLRDRIAPNTLLTINGDIPDRQTGLQLAEKYGIDGVMIGRGIFKNPF
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFHGAAELRNQLMSTKSTDEVRALLDKFEQR
>Mature_319_residues
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVRGRLIFTEDEQPIVAHIWGDN
PEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLILRPDVAAELIQAAKAGGLPVSVKTRLGFKEVNEWEEWLTH
IFKQDIANLSIHLRTREEMSQVAAHWELIPEIKKLRDRIAPNTLLTINGDIPDRQTGLQLAEKYGIDGVMIGRGIFKNPF
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFHGAAELRNQLMSTKSTDEVRALLDKFEQR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=322, Percent_Identity=25.4658385093168, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI239788483, Length=258, Percent_Identity=27.906976744186, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI239788462, Length=167, Percent_Identity=32.3353293413174, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=30.7053941908714, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789660, Length=238, Percent_Identity=29.4117647058824, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI145693211, Length=244, Percent_Identity=26.2295081967213, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=260, Percent_Identity=28.0769230769231, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=212, Percent_Identity=25.9433962264151, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=241, Percent_Identity=27.3858921161826, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=218, Percent_Identity=26.1467889908257, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921524, Length=236, Percent_Identity=26.6949152542373, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36415; Mature: 36415

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVR
CCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHC
GRLIFTEDEQPIVAHIWGDNPEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLI
CEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHCCCCEEEECCCCCCCHHCCCCCCCEE
LRPDVAAELIQAAKAGGLPVSVKTRLGFKEVNEWEEWLTHIFKQDIANLSIHLRTREEMS
ECCHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHH
QVAAHWELIPEIKKLRDRIAPNTLLTINGDIPDRQTGLQLAEKYGIDGVMIGRGIFKNPF
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFHGAAELRNQL
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
MSTKSTDEVRALLDKFEQR
HCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDNFWRDLPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNSDSYCHPEGMKSVR
CCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHC
GRLIFTEDEQPIVAHIWGDNPEYFRQMSIGMAELGFKGIDINMGCPVPNVASRGKGSGLI
CEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHCCCCEEEECCCCCCCHHCCCCCCCEE
LRPDVAAELIQAAKAGGLPVSVKTRLGFKEVNEWEEWLTHIFKQDIANLSIHLRTREEMS
ECCHHHHHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHH
QVAAHWELIPEIKKLRDRIAPNTLLTINGDIPDRQTGLQLAEKYGIDGVMIGRGIFKNPF
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPREHSSKEHLDLLRLQLDLQDQYAEVLPRSITGLHRFFKIYVKGFHGAAELRNQL
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
MSTKSTDEVRALLDKFEQR
HCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]