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The map label for this gene is purK [H]

Identifier: 152974109

GI number: 152974109

Start: 308789

End: 309940

Strand: Direct

Name: purK [H]

Synonym: Bcer98_0268

Alternate gene names: 152974109

Gene position: 308789-309940 (Clockwise)

Preceding gene: 152974108

Following gene: 152974110

Centisome position: 7.56

GC content: 37.76

Gene sequence:

>1152_bases
ATGACGAAAATCATTTTACCTGGACAAACAATTGGAATCATTGGCGGCGGTCAGCTTGGAAGAATGCTGGCTTTAGCAGC
GAAGGAAATGGGCTATAAAATCGCTGTTTTAGATCCAATAAGACATTCTCCATGTGCACAAGTTGCTGATATTGAAATTG
TAGCCGCTTATGACGATTTAAAAGCGATACAGCATTTGGCGGAAATAAGCGATGTTGTTACATATGAATTTGAGAACATT
AATTATAGATGTTTACAGTGGCTTGAGAAGTATGCATATTTGCCACAAGGGAGCCAATTACTATTCAAAACCCAAAATCG
TTTTACAGAAAAGAATACGATTGTAGAAGCTGGTTTGCCGGTAGCACCATATCGTCTCATTCAAAAGCAAAATGAGCTAC
TTACGGCAATAAGGGAACTGTCTTTCCCTTGTGTATTAAAAACAACAACGGGAGGATACGACGGCAAAGGTCAGGTTGTT
TTAAGAAGCGAAGCTGATATTACAAGAGCAAGTGAACTGATTAGTAAATCTGAATGTATTTTAGAAAAATGGGTGCCATT
TGAAAAAGAAATATCTGTTATTGTAACGCGCAGTGTAAGCGGGGAAACAAAGGTATTTCCAGTAGCTGAAAATATTCACG
TGAATAACATCTTACATGAATCAATCGTACCAGCTCGCATTACAGCAAACCTTTCCCAAAAGGGAATTCAATATGCACAG
ATACTCGCGGATGAACTTCAGTTAGTGGGAACACTAGCAGTAGAAATGTTTGCTACAGTTGATGGTGAAATTTATATTAA
TGAATTGGCTCCACGTCCTCACAATTCCGGTCATTATACAATTGATGCATGTGAAACGAGTCAGTTTAGACAGCATATTC
GAGCAATCTGTAATTTACCTCTTGGGGAAACAAATTTGTTAAAACCAGTTGTCATGGTAAACATTTTAGGCGAACATATA
GAAGGGGTCCTAAGACAAGTGAATAGATTAACCGGGTGTTATTTACACTTATATGGAAAAGAAGAAGCAAAATTACAGCG
TAAAATGGGACATGTTAATATTTTGAATGAAAATATCGAAGTGGCTCTAGAAAAAGCGCGTAGTTTGCATATTTGGGACC
ATCAAGAACAACTGTTGGAGGGAAAAAGATGA

Upstream 100 bases:

>100_bases
CGGCACAAATACTTGGCTCATTTTATGATGACATACATGATGCATTAGAACTGAGACGAGAAGCAATTGAGAAAAATGTG
CGCGAAGGTAGTGAGCTAAT

Downstream 100 bases:

>100_bases
TTAGTCGTTATACACGCCCTGAGATGGGTGCGATTTGGACGGAAGAAAATAAATTTAAAGCATGGTTGGAAGTGGAGATT
TTAGCTTGTGAAGCATGGGC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTKIILPGQTIGIIGGGQLGRMLALAAKEMGYKIAVLDPIRHSPCAQVADIEIVAAYDDLKAIQHLAEISDVVTYEFENI
NYRCLQWLEKYAYLPQGSQLLFKTQNRFTEKNTIVEAGLPVAPYRLIQKQNELLTAIRELSFPCVLKTTTGGYDGKGQVV
LRSEADITRASELISKSECILEKWVPFEKEISVIVTRSVSGETKVFPVAENIHVNNILHESIVPARITANLSQKGIQYAQ
ILADELQLVGTLAVEMFATVDGEIYINELAPRPHNSGHYTIDACETSQFRQHIRAICNLPLGETNLLKPVVMVNILGEHI
EGVLRQVNRLTGCYLHLYGKEEAKLQRKMGHVNILNENIEVALEKARSLHIWDHQEQLLEGKR

Sequences:

>Translated_383_residues
MTKIILPGQTIGIIGGGQLGRMLALAAKEMGYKIAVLDPIRHSPCAQVADIEIVAAYDDLKAIQHLAEISDVVTYEFENI
NYRCLQWLEKYAYLPQGSQLLFKTQNRFTEKNTIVEAGLPVAPYRLIQKQNELLTAIRELSFPCVLKTTTGGYDGKGQVV
LRSEADITRASELISKSECILEKWVPFEKEISVIVTRSVSGETKVFPVAENIHVNNILHESIVPARITANLSQKGIQYAQ
ILADELQLVGTLAVEMFATVDGEIYINELAPRPHNSGHYTIDACETSQFRQHIRAICNLPLGETNLLKPVVMVNILGEHI
EGVLRQVNRLTGCYLHLYGKEEAKLQRKMGHVNILNENIEVALEKARSLHIWDHQEQLLEGKR
>Mature_382_residues
TKIILPGQTIGIIGGGQLGRMLALAAKEMGYKIAVLDPIRHSPCAQVADIEIVAAYDDLKAIQHLAEISDVVTYEFENIN
YRCLQWLEKYAYLPQGSQLLFKTQNRFTEKNTIVEAGLPVAPYRLIQKQNELLTAIRELSFPCVLKTTTGGYDGKGQVVL
RSEADITRASELISKSECILEKWVPFEKEISVIVTRSVSGETKVFPVAENIHVNNILHESIVPARITANLSQKGIQYAQI
LADELQLVGTLAVEMFATVDGEIYINELAPRPHNSGHYTIDACETSQFRQHIRAICNLPLGETNLLKPVVMVNILGEHIE
GVLRQVNRLTGCYLHLYGKEEAKLQRKMGHVNILNENIEVALEKARSLHIWDHQEQLLEGKR

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=350, Percent_Identity=29.7142857142857, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1788155, Length=369, Percent_Identity=24.1192411924119, Blast_Score=102, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324702, Length=375, Percent_Identity=35.2, Blast_Score=220, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 42912; Mature: 42781

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIILPGQTIGIIGGGQLGRMLALAAKEMGYKIAVLDPIRHSPCAQVADIEIVAAYDDL
CCEEEECCCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEEEHHHH
KAIQHLAEISDVVTYEFENINYRCLQWLEKYAYLPQGSQLLFKTQNRFTEKNTIVEAGLP
HHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCEEECCCC
VAPYRLIQKQNELLTAIRELSFPCVLKTTTGGYDGKGQVVLRSEADITRASELISKSECI
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
LEKWVPFEKEISVIVTRSVSGETKVFPVAENIHVNNILHESIVPARITANLSQKGIQYAQ
HHHCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCHHEECCHHHHHHHHHH
ILADELQLVGTLAVEMFATVDGEIYINELAPRPHNSGHYTIDACETSQFRQHIRAICNLP
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHCCC
LGETNLLKPVVMVNILGEHIEGVLRQVNRLTGCYLHLYGKEEAKLQRKMGHVNILNENIE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCEEEECCHHH
VALEKARSLHIWDHQEQLLEGKR
HHHHHHCCEEEECCHHHHHCCCC
>Mature Secondary Structure 
TKIILPGQTIGIIGGGQLGRMLALAAKEMGYKIAVLDPIRHSPCAQVADIEIVAAYDDL
CEEEECCCEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEEEHHHH
KAIQHLAEISDVVTYEFENINYRCLQWLEKYAYLPQGSQLLFKTQNRFTEKNTIVEAGLP
HHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCEEECCCC
VAPYRLIQKQNELLTAIRELSFPCVLKTTTGGYDGKGQVVLRSEADITRASELISKSECI
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
LEKWVPFEKEISVIVTRSVSGETKVFPVAENIHVNNILHESIVPARITANLSQKGIQYAQ
HHHCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHCCCCHHEECCHHHHHHHHHH
ILADELQLVGTLAVEMFATVDGEIYINELAPRPHNSGHYTIDACETSQFRQHIRAICNLP
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHCCC
LGETNLLKPVVMVNILGEHIEGVLRQVNRLTGCYLHLYGKEEAKLQRKMGHVNILNENIE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCEEEECCHHH
VALEKARSLHIWDHQEQLLEGKR
HHHHHHCCEEEECCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]