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The map label for this gene is tilS [H]

Identifier: 152973911

GI number: 152973911

Start: 67860

End: 69290

Strand: Direct

Name: tilS [H]

Synonym: Bcer98_0058

Alternate gene names: 152973911

Gene position: 67860-69290 (Clockwise)

Preceding gene: 152973910

Following gene: 152973912

Centisome position: 1.66

GC content: 35.78

Gene sequence:

>1431_bases
TTGAAAAATTCATTTGTTGAAAAAGTGGATAGATTTGCAAAGCAGCATGGTGTATTAGAGGAACATTCAACCATTGTTGT
AGGAGTTTCAGGAGGACCTGACTCCTTAGCTCTACTATATTATTTGTTAGAAAAAAGAGAAGCGAAGAATTTAACAATTG
TTGTCGCGCATGTAGATCATATGTTTAGAGGAGATGAATCCTACGAAGATTTACAATTTGTAGAAAATCTTTGCCATGAA
CTTGGGATTCTTTGTGAAACGATAAGAATTAATGTTTTGCAATATCAACAGCAACATGGAATGAATGCGCAGGTAGCTGC
AAGGGAATGTCGATATGCATTTTTGGAAAGAATAATGAGGAAATATGATGCAGGATATGTCGCGCTTGGTCATCATGGAG
ATGATCAAGTAGAAACGATTCTGATGCGTCTCGTGCGCGGGAGTACTCCGCAAGGATATGCTGGTATTGCTGTGAAGCGC
CCTTTTCATAATGGATATTTAATTAGGCCTTTATTGGCAGTGACAAAGGAAGAAATTTTAGATTACTGCGACCAGTTAGG
CATTATGCCGCGTATAGATTCAAGTAATAAAAAAGAAGTATATACAAGAAATCGACTACGTAAGTATGTTTTGCCTTATT
TAAAAGAAGAAAATCCTCAGGTGCATGAGCACTTTCAAAAATTTAGTCAGCTTATGCAAGAGGATGAGGCCTATTTGCAG
GAATTAGCTTTTGAAAAGATGAATAAAGTAATTACGAAAAAAAGTGATAAATATCTTGTCTTATCAATTCCTGCCTTTGA
ATCCTTGTCTATGCCTTTACAAAGGAGAGGGATTCAACTAATATTAAACTATCTTTATGAATATAAGATTCCATCTTCGC
TTTCTTCTATACATATTGACAAAGTAATTGCGTTTTTCAAACGGATACAACCATCAGGATCGCTAGATTTCCCAGAAGGT
TTAAAAATTGTTCGTTCATATGAAGAATGCAGTTTCCGATTTGAACAAGAAATTGTTACCCCTTTTTCTGAAGTTTTATC
CATACCTGGTGCAGTTACATTAGCGAATGGAGATAAGCTTATAGCAGAAGTAATAGAGGAAATACCGAGTGACATGAATG
AAATGGTATTTGTCGCTAAGTATAATGATATGTCATATCCTCTTTTGGTTCGCTCTCGAGAAAATGGAGATCGTATGTCA
ATACAAGGTATGAGCGGCACAAAAAAGATAAAAACTATCTTTATCGAAGCGAAAGTACCAAAGGAAAAAAGAGAAGAATG
GCCGATTGTTTGTGATGCAAGTGGAAACATTATTTGGGTTCCCTTGTTGAAACGATCTGCATTTGCTATTTCGCCAAAAG
TAGCAAACAAAGATAAATATATAATTATTCACTACAAAAGCAAGGAGTCTTCCGGGAGGATAATGAAATGA

Upstream 100 bases:

>100_bases
TTTGTACATCTTTTTTTTGCACAAAGTTTGTAAAAGTTGTACCATTATAATCAAGGTAAAGTATGTTATTGTTTGGCTTT
AATGAAGGGTGAATGCGAAG

Downstream 100 bases:

>100_bases
TGAATCAAGATATTGAAAAAGTATTAATTTCTGAGGAGCAAATACAGAAAAAGGTGCGCGAACTAGGTGCAATCATTGCG
GAGGAGTATAAGAATACCGT

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKNSFVEKVDRFAKQHGVLEEHSTIVVGVSGGPDSLALLYYLLEKREAKNLTIVVAHVDHMFRGDESYEDLQFVENLCHE
LGILCETIRINVLQYQQQHGMNAQVAARECRYAFLERIMRKYDAGYVALGHHGDDQVETILMRLVRGSTPQGYAGIAVKR
PFHNGYLIRPLLAVTKEEILDYCDQLGIMPRIDSSNKKEVYTRNRLRKYVLPYLKEENPQVHEHFQKFSQLMQEDEAYLQ
ELAFEKMNKVITKKSDKYLVLSIPAFESLSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIAFFKRIQPSGSLDFPEG
LKIVRSYEECSFRFEQEIVTPFSEVLSIPGAVTLANGDKLIAEVIEEIPSDMNEMVFVAKYNDMSYPLLVRSRENGDRMS
IQGMSGTKKIKTIFIEAKVPKEKREEWPIVCDASGNIIWVPLLKRSAFAISPKVANKDKYIIIHYKSKESSGRIMK

Sequences:

>Translated_476_residues
MKNSFVEKVDRFAKQHGVLEEHSTIVVGVSGGPDSLALLYYLLEKREAKNLTIVVAHVDHMFRGDESYEDLQFVENLCHE
LGILCETIRINVLQYQQQHGMNAQVAARECRYAFLERIMRKYDAGYVALGHHGDDQVETILMRLVRGSTPQGYAGIAVKR
PFHNGYLIRPLLAVTKEEILDYCDQLGIMPRIDSSNKKEVYTRNRLRKYVLPYLKEENPQVHEHFQKFSQLMQEDEAYLQ
ELAFEKMNKVITKKSDKYLVLSIPAFESLSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIAFFKRIQPSGSLDFPEG
LKIVRSYEECSFRFEQEIVTPFSEVLSIPGAVTLANGDKLIAEVIEEIPSDMNEMVFVAKYNDMSYPLLVRSRENGDRMS
IQGMSGTKKIKTIFIEAKVPKEKREEWPIVCDASGNIIWVPLLKRSAFAISPKVANKDKYIIIHYKSKESSGRIMK
>Mature_476_residues
MKNSFVEKVDRFAKQHGVLEEHSTIVVGVSGGPDSLALLYYLLEKREAKNLTIVVAHVDHMFRGDESYEDLQFVENLCHE
LGILCETIRINVLQYQQQHGMNAQVAARECRYAFLERIMRKYDAGYVALGHHGDDQVETILMRLVRGSTPQGYAGIAVKR
PFHNGYLIRPLLAVTKEEILDYCDQLGIMPRIDSSNKKEVYTRNRLRKYVLPYLKEENPQVHEHFQKFSQLMQEDEAYLQ
ELAFEKMNKVITKKSDKYLVLSIPAFESLSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVIAFFKRIQPSGSLDFPEG
LKIVRSYEECSFRFEQEIVTPFSEVLSIPGAVTLANGDKLIAEVIEEIPSDMNEMVFVAKYNDMSYPLLVRSRENGDRMS
IQGMSGTKKIKTIFIEAKVPKEKREEWPIVCDASGNIIWVPLLKRSAFAISPKVANKDKYIIIHYKSKESSGRIMK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=214, Percent_Identity=31.7757009345794, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 54735; Mature: 54735

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSFVEKVDRFAKQHGVLEEHSTIVVGVSGGPDSLALLYYLLEKREAKNLTIVVAHVDH
CCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEHHH
MFRGDESYEDLQFVENLCHELGILCETIRINVLQYQQQHGMNAQVAARECRYAFLERIMR
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KYDAGYVALGHHGDDQVETILMRLVRGSTPQGYAGIAVKRPFHNGYLIRPLLAVTKEEIL
HHCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
DYCDQLGIMPRIDSSNKKEVYTRNRLRKYVLPYLKEENPQVHEHFQKFSQLMQEDEAYLQ
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ELAFEKMNKVITKKSDKYLVLSIPAFESLSMPLQRRGIQLILNYLYEYKIPSSLSSIHID
HHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KVIAFFKRIQPSGSLDFPEGLKIVRSYEECSFRFEQEIVTPFSEVLSIPGAVTLANGDKL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEECCHHH
IAEVIEEIPSDMNEMVFVAKYNDMSYPLLVRSRENGDRMSIQGMSGTKKIKTIFIEAKVP
HHHHHHHHCCCHHHEEEEEEECCCCCCEEEEECCCCCEEEEECCCCCCEEEEEEEEECCC
KEKREEWPIVCDASGNIIWVPLLKRSAFAISPKVANKDKYIIIHYKSKESSGRIMK
HHHHCCCCEEEECCCCEEEEEECCCCCEEECCCCCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKNSFVEKVDRFAKQHGVLEEHSTIVVGVSGGPDSLALLYYLLEKREAKNLTIVVAHVDH
CCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEHHH
MFRGDESYEDLQFVENLCHELGILCETIRINVLQYQQQHGMNAQVAARECRYAFLERIMR
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KYDAGYVALGHHGDDQVETILMRLVRGSTPQGYAGIAVKRPFHNGYLIRPLLAVTKEEIL
HHCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
DYCDQLGIMPRIDSSNKKEVYTRNRLRKYVLPYLKEENPQVHEHFQKFSQLMQEDEAYLQ
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ELAFEKMNKVITKKSDKYLVLSIPAFESLSMPLQRRGIQLILNYLYEYKIPSSLSSIHID
HHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KVIAFFKRIQPSGSLDFPEGLKIVRSYEECSFRFEQEIVTPFSEVLSIPGAVTLANGDKL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEECCHHH
IAEVIEEIPSDMNEMVFVAKYNDMSYPLLVRSRENGDRMSIQGMSGTKKIKTIFIEAKVP
HHHHHHHHCCCHHHEEEEEEECCCCCCEEEEECCCCCEEEEECCCCCCEEEEEEEEECCC
KEKREEWPIVCDASGNIIWVPLLKRSAFAISPKVANKDKYIIIHYKSKESSGRIMK
HHHHCCCCEEEECCCCEEEEEECCCCCEEECCCCCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]