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The map label for this gene is metG1 [H]

Identifier: 152973886

GI number: 152973886

Start: 41718

End: 43697

Strand: Direct

Name: metG1 [H]

Synonym: Bcer98_0033

Alternate gene names: 152973886

Gene position: 41718-43697 (Clockwise)

Preceding gene: 152973884

Following gene: 152973887

Centisome position: 1.02

GC content: 34.9

Gene sequence:

>1980_bases
ATGACAGAGGAAAAAAAATCCTTTTATATTACTACTCCAATTTATTACCCAAGCGGAAAGCTCCATATTGGACATGCTTA
TACAACAGTAGCAGGAGATGCAATGGCACGCTACAAACGCATGCAAGGGTATGATGTTCATTACTTAACAGGTACTGATG
AACATGGACAAAAGATTCAAAAGAAAGCAGAAGAGTTACAAGTAACACCACAAGCTTATGTTGATGACATTGTTGCGGGA
ATTAAAGATTTATGGAATAAAATGGATATTTCTTATGATGATTTTATTCGCACAACAGAAGATCGTCATAAAGAAGTAGT
AGAAAAGATTTTCAAACAATTAGTTGATCAAGGTGATATTTACTTAGATGAATACGAAGGATGGTACTCTGTACAGGATG
AAACATTCTATACGGAACATCAATTAGTTGATCCAATTATGGAAAATGGTAAAGTAGTTGGTGGAAAAAGCCCTGATAGT
GGACATGCTGTAGAACTTGTTCGTGAAGCATCCTATTTCTTTAGAATGGGTAAATATGTAGATCGACTATTAAAGTTCTA
TGAAGAGAATCCTCATTTCATTCAGCCAGAATCACGTAAAAATGAAATGATTAATAACTTCATTAAACCAGGATTAGAAG
ATTTAGCTGTTTCACGTACTTCATTTGACTGGGGAATTCGTGTCCCAGGAAATCCCAAACATGTTATTTACGTATGGGTA
GATGCATTATCAAATTATATTACAGCATTAGGATATGGAACAGAAAATGCAGAGAAGTATAAAAAGTTTTGGCCAGCAGA
TGTTCATTTAGTAGGAAAAGAAATTGTTCGTTTCCACACAATTTATTGGCCAATTATTTTAATGGCACTAGATTTACCAC
TTCCGAAGAAAGTATTTGCTCATGGCTGGATTTTAATGAAAGATGGAAAAATGAGCAAATCGAAAGGGAATGTCGTTGAT
CCTGTTACATTAATTGATCGTTATGGATTAGATGCATTACGTTATTACTTACTTCGCGAAGTTCCATTTGGATCAGATGG
TGTATTTACACCGGAAGGTTTTGTTGAACGGATTAACTTTGACCTTGCAAATGACTTAGGGAACTTATTAAATCGTACAG
TTGCAATGATCGATAAGTACTTTGACGGTCAAATCCCTGCATTTAAAGAAAAGGTCACAGAATTTGACGAAGCGTTAGTA
AATTTTGCAACCGATACATTACAAAAAGTAGAAGAAGCAATGGAAAATATGGAATTCTCGGTAGCATTAAGTTCTATTTG
GCAATTCATTAGTCGTACGAATAAATATATCGATGAAACACAGCCATGGGTATTAGCAAAAGATGAGAAAGATCGAGAGA
AACTGGCATCTGTTATGGCACATTTAGCAGAAATGTTACGTCAAACAAGTATCATGCTTATGCCATTCTTAACAACAACA
TCAGGTAAAATCTTTGAACAACTTGGTATTACTGATGAGGCATATCAATCTTGGGAGAGCCTTTCTACAATTGGATGTAT
CCCAACTGGTACAAAAGTTGTAAAAGGACAACCAATTTTCCCACGTTTAGAAGTAGAAGTAGAAGTTGCTTATATTAAAG
AACAAATGAAGGGTTCTGCTCCTAAAGTTGAAGAGAAGAAAGAAGAACCGAAAGCAGAAGAAATTACGATTGATGATTTC
TTTAAAGTAGAATTGCGTGTGGCAGAAGTAATCGAAGCAGAACCGGTGAAAAAAGCAGATAAATTACTAAAAATCCAATT
GGATTTAGGGACAGAAAAACGTCAAGTTGTATCTGGAATTGCGAAATTCTATACACCAGAAGACTTAAAAGGTAAAAAAG
TTATTTGTGTAACGAATTTAAAACCAGTGAAATTACGTGGTGAATTATCACAAGGTATGATTTTAGCGGGTGAAGAAGAC
GGTGTCCTATCGTTAGCAACAGTTGATCAAAACTTACCAAATGGTACAAAAATCAAGTAA

Upstream 100 bases:

>100_bases
AATAGATGATAAAGATATCGAGCATTAATTCGTTTTTATGCGTCAGTTTTTGATATACTGTTTATAAATACATATGAGGT
CATTTGGGAGGTTCAAAATA

Downstream 100 bases:

>100_bases
TTATGACAAAAAAAGAGGTGTTTCACGTGTAACATTCGTGAAGCATCTTTTTTCTTACTAAGGATTTCACTTTTGACTTA
TATTGTAATATTACCGGCAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase 1; MetRS 1 [H]

Number of amino acids: Translated: 659; Mature: 658

Protein sequence:

>659_residues
MTEEKKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYDVHYLTGTDEHGQKIQKKAEELQVTPQAYVDDIVAG
IKDLWNKMDISYDDFIRTTEDRHKEVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMENGKVVGGKSPDS
GHAVELVREASYFFRMGKYVDRLLKFYEENPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGIRVPGNPKHVIYVWV
DALSNYITALGYGTENAEKYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVD
PVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFDGQIPAFKEKVTEFDEALV
NFATDTLQKVEEAMENMEFSVALSSIWQFISRTNKYIDETQPWVLAKDEKDREKLASVMAHLAEMLRQTSIMLMPFLTTT
SGKIFEQLGITDEAYQSWESLSTIGCIPTGTKVVKGQPIFPRLEVEVEVAYIKEQMKGSAPKVEEKKEEPKAEEITIDDF
FKVELRVAEVIEAEPVKKADKLLKIQLDLGTEKRQVVSGIAKFYTPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEED
GVLSLATVDQNLPNGTKIK

Sequences:

>Translated_659_residues
MTEEKKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYDVHYLTGTDEHGQKIQKKAEELQVTPQAYVDDIVAG
IKDLWNKMDISYDDFIRTTEDRHKEVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMENGKVVGGKSPDS
GHAVELVREASYFFRMGKYVDRLLKFYEENPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGIRVPGNPKHVIYVWV
DALSNYITALGYGTENAEKYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVD
PVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFDGQIPAFKEKVTEFDEALV
NFATDTLQKVEEAMENMEFSVALSSIWQFISRTNKYIDETQPWVLAKDEKDREKLASVMAHLAEMLRQTSIMLMPFLTTT
SGKIFEQLGITDEAYQSWESLSTIGCIPTGTKVVKGQPIFPRLEVEVEVAYIKEQMKGSAPKVEEKKEEPKAEEITIDDF
FKVELRVAEVIEAEPVKKADKLLKIQLDLGTEKRQVVSGIAKFYTPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEED
GVLSLATVDQNLPNGTKIK
>Mature_658_residues
TEEKKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYDVHYLTGTDEHGQKIQKKAEELQVTPQAYVDDIVAGI
KDLWNKMDISYDDFIRTTEDRHKEVVEKIFKQLVDQGDIYLDEYEGWYSVQDETFYTEHQLVDPIMENGKVVGGKSPDSG
HAVELVREASYFFRMGKYVDRLLKFYEENPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGIRVPGNPKHVIYVWVD
ALSNYITALGYGTENAEKYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDP
VTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINFDLANDLGNLLNRTVAMIDKYFDGQIPAFKEKVTEFDEALVN
FATDTLQKVEEAMENMEFSVALSSIWQFISRTNKYIDETQPWVLAKDEKDREKLASVMAHLAEMLRQTSIMLMPFLTTTS
GKIFEQLGITDEAYQSWESLSTIGCIPTGTKVVKGQPIFPRLEVEVEVAYIKEQMKGSAPKVEEKKEEPKAEEITIDDFF
KVELRVAEVIEAEPVKKADKLLKIQLDLGTEKRQVVSGIAKFYTPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEEDG
VLSLATVDQNLPNGTKIK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=504, Percent_Identity=34.9206349206349, Blast_Score=300, Evalue=3e-81,
Organism=Homo sapiens, GI14043022, Length=655, Percent_Identity=26.2595419847328, Blast_Score=182, Evalue=6e-46,
Organism=Escherichia coli, GI1788432, Length=695, Percent_Identity=29.0647482014388, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1789455, Length=108, Percent_Identity=36.1111111111111, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1786861, Length=208, Percent_Identity=25.9615384615385, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI212645096, Length=497, Percent_Identity=33.8028169014084, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI71992781, Length=387, Percent_Identity=32.8165374677003, Blast_Score=198, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17540794, Length=600, Percent_Identity=25.8333333333333, Blast_Score=168, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6321610, Length=525, Percent_Identity=30.8571428571429, Blast_Score=268, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6321704, Length=526, Percent_Identity=24.7148288973384, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323414, Length=226, Percent_Identity=28.3185840707965, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21358483, Length=566, Percent_Identity=32.6855123674912, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24655311, Length=563, Percent_Identity=26.4653641207815, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75250; Mature: 75119

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEKKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYDVHYLTGTDEHGQKIQ
CCCCCCCEEEEECEEECCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
KKAEELQVTPQAYVDDIVAGIKDLWNKMDISYDDFIRTTEDRHKEVVEKIFKQLVDQGDI
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCE
YLDEYEGWYSVQDETFYTEHQLVDPIMENGKVVGGKSPDSGHAVELVREASYFFRMGKYV
EEECCCCEEECCCCEEEHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
DRLLKFYEENPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGIRVPGNPKHVIYVWV
HHHHHHHCCCCCEECCCHHHHHHHHHHHCCCHHHHHHCCCCCCCEEECCCCCCEEEEEEH
DALSNYITALGYGTENAEKYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFA
HHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HGWILMKDGKMSKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINF
CCEEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCH
DLANDLGNLLNRTVAMIDKYFDGQIPAFKEKVTEFDEALVNFATDTLQKVEEAMENMEFS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VALSSIWQFISRTNKYIDETQPWVLAKDEKDREKLASVMAHLAEMLRQTSIMLMPFLTTT
HHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEEHHCC
SGKIFEQLGITDEAYQSWESLSTIGCIPTGTKVVKGQPIFPRLEVEVEVAYIKEQMKGSA
CHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEHHHHHHHHHCCCC
PKVEEKKEEPKAEEITIDDFFKVELRVAEVIEAEPVKKADKLLKIQLDLGTEKRQVVSGI
CCCHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEECCCCHHHHHHHHH
AKFYTPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEEDGVLSLATVDQNLPNGTKIK
HHHCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEECCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TEEKKSFYITTPIYYPSGKLHIGHAYTTVAGDAMARYKRMQGYDVHYLTGTDEHGQKIQ
CCCCCCEEEEECEEECCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
KKAEELQVTPQAYVDDIVAGIKDLWNKMDISYDDFIRTTEDRHKEVVEKIFKQLVDQGDI
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCE
YLDEYEGWYSVQDETFYTEHQLVDPIMENGKVVGGKSPDSGHAVELVREASYFFRMGKYV
EEECCCCEEECCCCEEEHHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
DRLLKFYEENPHFIQPESRKNEMINNFIKPGLEDLAVSRTSFDWGIRVPGNPKHVIYVWV
HHHHHHHCCCCCEECCCHHHHHHHHHHHCCCHHHHHHCCCCCCCEEECCCCCCEEEEEEH
DALSNYITALGYGTENAEKYKKFWPADVHLVGKEIVRFHTIYWPIILMALDLPLPKKVFA
HHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
HGWILMKDGKMSKSKGNVVDPVTLIDRYGLDALRYYLLREVPFGSDGVFTPEGFVERINF
CCEEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCH
DLANDLGNLLNRTVAMIDKYFDGQIPAFKEKVTEFDEALVNFATDTLQKVEEAMENMEFS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VALSSIWQFISRTNKYIDETQPWVLAKDEKDREKLASVMAHLAEMLRQTSIMLMPFLTTT
HHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEEHHCC
SGKIFEQLGITDEAYQSWESLSTIGCIPTGTKVVKGQPIFPRLEVEVEVAYIKEQMKGSA
CHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEEHHHHHHHHHCCCC
PKVEEKKEEPKAEEITIDDFFKVELRVAEVIEAEPVKKADKLLKIQLDLGTEKRQVVSGI
CCCHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEECCCCHHHHHHHHH
AKFYTPEDLKGKKVICVTNLKPVKLRGELSQGMILAGEEDGVLSLATVDQNLPNGTKIK
HHHCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEECCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]