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The map label for this gene is srlE [H]

Identifier: 152971560

GI number: 152971560

Start: 3333248

End: 3334228

Strand: Direct

Name: srlE [H]

Synonym: KPN_03038

Alternate gene names: 152971560

Gene position: 3333248-3334228 (Clockwise)

Preceding gene: 152971559

Following gene: 152971561

Centisome position: 62.71

GC content: 62.69

Gene sequence:

>981_bases
ATGACCCGTATTCGTATAGAGAAAGGCACCGGCGGCTGGGGCGGCCCGCTTGAGCTCGACGCCGTCAAAGGCAAAAAGAT
CGTCTATATCACCGCCGGCACCCGCCCGGCGATCGTTGACCGCATCGCCAGCCTGACCGGCTGGCAGCCGGTGGACGGGT
TTAAAGAGGGGGAACCAGCGGAAAGCGACATCGGCGTGGCGATTATCGACTGCGGGGGCACCCTGCGCTGCGGGCTCTAT
CCTAAGCGCCGCATCCCGACGGTGAATATCCATGCCACCGGCAAATCAGGGCCGCTGGCCCAGTACATCACGGAAGATAT
TTATGTCTCCGGCGTCAAAGAGGACAACATCCAGTGCATCGCTCCCGACAGCGCAGGGGTTTCCGAGCCGCCGGCGGAAA
ACAAGAGCGCGGGTCGCGATTACGACACCAGCAAGAAGATTACTGAACAGAGCGATGGCCTGCTGGCGAAGGTCGGGATG
GGCATGGGTTCGGTGGTGGCGGTGCTGTTCCAGGCCGGGCGCGACACCATTGATACGGTGCTGAAAACCATTCTGCCGTT
TATGGCTTTCGTCTCGGCGCTGATCGGCATCATCATGGCCTCCGGCATCGGCGACTGGATCGCCCACGGCCTGGCGCCGC
TCGCCAGCCATCCGCTGGGGCTGGTAACCCTGGCGCTGATTTGCTCTTTCCCGCTGCTGTCGCCGTTTCTCGGCCCCGGC
GCGGTTATTGCCCAGGTGATCGGCGTGCTGATCGGCGTTCAAATCGGCCTCGGCAACATTCCGCCGCACCTCGCCCTGCC
CGCCCTATTCGCCATTAACGCCCAGGCCGCCTGCGATTTTATTCCGGTAGGCCTGTCGCTAGCCGAAGCCCGTCAGGATA
CCGTGCGCGTCGGCGTGCCGTCGGTGCTGGTCAGCCGCTTTCTGACCGGCGCACCAACGGTGCTGATCGCGTGGTTCGTT
TCCGGATTTATTTACCAGTAA

Upstream 100 bases:

>100_bases
ACTTCTTCCGCGGCTGGGTCACCGACCTGACCACCTCGATTTTCGAGAGAAAAATGGCGATTCAGCTTTCCCAGAAAGTG
CATCTGTCAGGAGCGACATC

Downstream 100 bases:

>100_bases
GAGGTTCCTGCAGATGACTGTGATTTACCAGACCACCATTACGCACATCGGCGCCTGCGCGACGATGGCGCTGGAAGAGC
AAATGCTGATCACCTTCCGC

Product: PTS family enzyme IIBC, glucitol/sorbitol-specific

Products: NA

Alternate protein names: EII-Gut; PTS system glucitol/sorbitol-specific EIIB component [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTRIRIEKGTGGWGGPLELDAVKGKKIVYITAGTRPAIVDRIASLTGWQPVDGFKEGEPAESDIGVAIIDCGGTLRCGLY
PKRRIPTVNIHATGKSGPLAQYITEDIYVSGVKEDNIQCIAPDSAGVSEPPAENKSAGRDYDTSKKITEQSDGLLAKVGM
GMGSVVAVLFQAGRDTIDTVLKTILPFMAFVSALIGIIMASGIGDWIAHGLAPLASHPLGLVTLALICSFPLLSPFLGPG
AVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFV
SGFIYQ

Sequences:

>Translated_326_residues
MTRIRIEKGTGGWGGPLELDAVKGKKIVYITAGTRPAIVDRIASLTGWQPVDGFKEGEPAESDIGVAIIDCGGTLRCGLY
PKRRIPTVNIHATGKSGPLAQYITEDIYVSGVKEDNIQCIAPDSAGVSEPPAENKSAGRDYDTSKKITEQSDGLLAKVGM
GMGSVVAVLFQAGRDTIDTVLKTILPFMAFVSALIGIIMASGIGDWIAHGLAPLASHPLGLVTLALICSFPLLSPFLGPG
AVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFV
SGFIYQ
>Mature_325_residues
TRIRIEKGTGGWGGPLELDAVKGKKIVYITAGTRPAIVDRIASLTGWQPVDGFKEGEPAESDIGVAIIDCGGTLRCGLYP
KRRIPTVNIHATGKSGPLAQYITEDIYVSGVKEDNIQCIAPDSAGVSEPPAENKSAGRDYDTSKKITEQSDGLLAKVGMG
MGSVVAVLFQAGRDTIDTVLKTILPFMAFVSALIGIIMASGIGDWIAHGLAPLASHPLGLVTLALICSFPLLSPFLGPGA
VIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVS
GFIYQ

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3732

COG function: function code G; Phosphotransferase system sorbitol-specific component IIBC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994905, Length=326, Percent_Identity=88.0368098159509, Blast_Score=574, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011638
- InterPro:   IPR011618
- InterPro:   IPR004702 [H]

Pfam domain/function: PF07663 EIIBC-GUT_C; PF03612 EIIBC-GUT_N [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 34006; Mature: 33875

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00107 PROTEIN_KINASE_ATP ; PS51102 PTS_EIIB_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIRIEKGTGGWGGPLELDAVKGKKIVYITAGTRPAIVDRIASLTGWQPVDGFKEGEPA
CCEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCC
ESDIGVAIIDCGGTLRCGLYPKRRIPTVNIHATGKSGPLAQYITEDIYVSGVKEDNIQCI
CCCCCEEEEECCCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHEEECCCCCCCEEE
APDSAGVSEPPAENKSAGRDYDTSKKITEQSDGLLAKVGMGMGSVVAVLFQAGRDTIDTV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHH
LKTILPFMAFVSALIGIIMASGIGDWIAHGLAPLASHPLGLVTLALICSFPLLSPFLGPG
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCH
AVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEARQDTVRVGVP
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHCCEEECCHHHHHHHHHHHHCCHH
SVLVSRFLTGAPTVLIAWFVSGFIYQ
HHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRIRIEKGTGGWGGPLELDAVKGKKIVYITAGTRPAIVDRIASLTGWQPVDGFKEGEPA
CEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCC
ESDIGVAIIDCGGTLRCGLYPKRRIPTVNIHATGKSGPLAQYITEDIYVSGVKEDNIQCI
CCCCCEEEEECCCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHEEECCCCCCCEEE
APDSAGVSEPPAENKSAGRDYDTSKKITEQSDGLLAKVGMGMGSVVAVLFQAGRDTIDTV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHH
LKTILPFMAFVSALIGIIMASGIGDWIAHGLAPLASHPLGLVTLALICSFPLLSPFLGPG
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCH
AVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEARQDTVRVGVP
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHCCEEECCHHHHHHHHHHHHCCHH
SVLVSRFLTGAPTVLIAWFVSGFIYQ
HHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3553176; 9205837; 9278503 [H]