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The map label for this gene is nrdF [H]

Identifier: 152971534

GI number: 152971534

Start: 3305517

End: 3306479

Strand: Direct

Name: nrdF [H]

Synonym: KPN_03007

Alternate gene names: 152971534

Gene position: 3305517-3306479 (Clockwise)

Preceding gene: 152971533

Following gene: 152971535

Centisome position: 62.19

GC content: 57.11

Gene sequence:

>963_bases
ATGACCCATTTAACGCGCGTCAGCGCAATCAACTGGAACCGCATTGATGACGATAAAGATCTTGAGGTCTGGAACCGTCT
CACCAGCAACTTCTGGCTGCCGGAGAAAGTGCCGCTGTCGAACGACATTCCCGCCTGGCAAACCCTCAGCGCGGCGGAGC
AGCAGTTGACCATTCGCGTCTTTACCGGATTGACCCTGCTTGACACCATTCAGAACACCGTCGGCGCCCCGGCGCTAATG
GCGGACGCCTTAACCCCGCATGAGGAGGCGGTGCTGTCGAACATCAGCTTTATGGAGGCGGTTCATGCCCGCTCTTACAG
CTCCATCTTTTCCACGCTGTGCCACAGCAAGGAAGTGGATGCCGCCTTCGCCTGGAGCGAAAGCTGCGATCCCCTGCAGC
GCAAGGCGCAGCTGATGCTGGATTACTATCAGGCCGACGAGCCGCTGAAGAAAAAAATCGCCAGCGTGTTCCTCGAATCC
TTTCTCTTCTACTCCGGCTTCTGGCTGCCGATGTACTTCTCCAGCCGGGGAAAGCTGACCAACACTGCGGATCTGATCCG
CCTGATCATTCGCGATGAAGCGGTGCATGGCTATTACATCGGCTACAAGTACCAGAAAGGACTGGAGATCGTCAGCCCCG
GCAAGCGCGAAGAATTGAAAAATTTCGCCCTCGATCTGCTGATGGATCTTTACGACAACGAACTGGCCTACAGCCGGGAG
CTGTACGGCGAGAGCGGCTGGTTCGACGACGTCAGCGCTTTCCTGTGCTACAACGCCAACAAAGCGCTGATGAACCTCGG
CTACGAGGCGCTGTTCCCGGCGGAGATGGCGGCGGTCAATCCGGCAATCCTCGCAGCGCTGTCGCCCAACGCCGACGAAA
ACCATGACTTTTTCTCCGGTTCCGGCTCCTCCTACGTGATAGGAAAAATGGAAGAGACCGCCGACGACGATTGGGACTTT
TAA

Upstream 100 bases:

>100_bases
AAAGGCATCAAGACGCTCTACTACATCCGTCTGCGCCAGCTGGCGCTGGAAGGCACTGAAATTGAAGGCTGCGTGTCCTG
CGCGCTGTAAGGAGAACAGG

Downstream 100 bases:

>100_bases
CCGTACTTTCACCAGGAATATCGCAGGCGGATTCCTGGTGATTAATTTCACAAAATAGCGTTTAATCCACCAAATATTCT
CTCCATGACCTAACCGTCAT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: R2F protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTHLTRVSAINWNRIDDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLSAAEQQLTIRVFTGLTLLDTIQNTVGAPALM
ADALTPHEEAVLSNISFMEAVHARSYSSIFSTLCHSKEVDAAFAWSESCDPLQRKAQLMLDYYQADEPLKKKIASVFLES
FLFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKGLEIVSPGKREELKNFALDLLMDLYDNELAYSRE
LYGESGWFDDVSAFLCYNANKALMNLGYEALFPAEMAAVNPAILAALSPNADENHDFFSGSGSSYVIGKMEETADDDWDF

Sequences:

>Translated_320_residues
MTHLTRVSAINWNRIDDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLSAAEQQLTIRVFTGLTLLDTIQNTVGAPALM
ADALTPHEEAVLSNISFMEAVHARSYSSIFSTLCHSKEVDAAFAWSESCDPLQRKAQLMLDYYQADEPLKKKIASVFLES
FLFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKGLEIVSPGKREELKNFALDLLMDLYDNELAYSRE
LYGESGWFDDVSAFLCYNANKALMNLGYEALFPAEMAAVNPAILAALSPNADENHDFFSGSGSSYVIGKMEETADDDWDF
>Mature_319_residues
THLTRVSAINWNRIDDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLSAAEQQLTIRVFTGLTLLDTIQNTVGAPALMA
DALTPHEEAVLSNISFMEAVHARSYSSIFSTLCHSKEVDAAFAWSESCDPLQRKAQLMLDYYQADEPLKKKIASVFLESF
LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKGLEIVSPGKREELKNFALDLLMDLYDNELAYSREL
YGESGWFDDVSAFLCYNANKALMNLGYEALFPAEMAAVNPAILAALSPNADENHDFFSGSGSSYVIGKMEETADDDWDF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1789031, Length=317, Percent_Identity=82.9652996845426, Blast_Score=558, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 36198; Mature: 36067

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHLTRVSAINWNRIDDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLSAAEQQLTIRV
CCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHEEEHH
FTGLTLLDTIQNTVGAPALMADALTPHEEAVLSNISFMEAVHARSYSSIFSTLCHSKEVD
HHCHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AAFAWSESCDPLQRKAQLMLDYYQADEPLKKKIASVFLESFLFYSGFWLPMYFSSRGKLT
HHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCC
NTADLIRLIIRDEAVHGYYIGYKYQKGLEIVSPGKREELKNFALDLLMDLYDNELAYSRE
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
LYGESGWFDDVSAFLCYNANKALMNLGYEALFPAEMAAVNPAILAALSPNADENHDFFSG
HCCCCCCHHHHHHHHHCCCCHHHHHCCHHHHCCHHHHHCCHHHEEEECCCCCCCCCCCCC
SGSSYVIGKMEETADDDWDF
CCCEEEEECCHHCCCCCCCC
>Mature Secondary Structure 
THLTRVSAINWNRIDDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLSAAEQQLTIRV
CCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHEEEHH
FTGLTLLDTIQNTVGAPALMADALTPHEEAVLSNISFMEAVHARSYSSIFSTLCHSKEVD
HHCHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AAFAWSESCDPLQRKAQLMLDYYQADEPLKKKIASVFLESFLFYSGFWLPMYFSSRGKLT
HHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCC
NTADLIRLIIRDEAVHGYYIGYKYQKGLEIVSPGKREELKNFALDLLMDLYDNELAYSRE
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
LYGESGWFDDVSAFLCYNANKALMNLGYEALFPAEMAAVNPAILAALSPNADENHDFFSG
HCCCCCCHHHHHHHHHCCCCHHHHHCCHHHHCCHHHHHCCHHHEEEECCCCCCCCCCCCC
SGSSYVIGKMEETADDDWDF
CCCEEEEECCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8195103; 11677609; 2691838; 2548994; 9748343 [H]