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The map label for this gene is nrdE [H]

Identifier: 152971533

GI number: 152971533

Start: 3303425

End: 3305506

Strand: Direct

Name: nrdE [H]

Synonym: KPN_03006

Alternate gene names: 152971533

Gene position: 3303425-3305506 (Clockwise)

Preceding gene: 152971532

Following gene: 152971534

Centisome position: 62.15

GC content: 61.58

Gene sequence:

>2082_bases
ATGCTGAATTTGTACGACAGCGAGGGCCGCATCCCGTTTGAGAAAGATCGCCAGGCGGTTGAGGCCTTTATGGCGACCCA
GGTTCAGCCCAACGCCCTCACCTTCCCCAGCCAGGAGGATAAGCTCAGCTGGCTCGTCAGCGAGGGCTATTACGATCCGC
AGGTGCTGGCCGGCTATGACCGCGGGTTTGTGCTGGCGTTGTTCGCCCATGCCCGGCGCGCTCCGTTTCGCTTTCAGACC
TTCCTCGGCGCCTGGAAGTTCTATACCAGCTATGCGCTGAAGACCTTTGACGGCAAGCACTACCTCGAGGATTTCGCCGA
ACGCAGCGTGATGGTGGCCCTGACGCTGGCCCGGGGCGATGAACAGCAGGCGCGGCAGCTGACGGAGGAGATACTCAGCG
GCCGCTTTCAGCCCGCCACACCGACCTTCCTTAACGCTGGCAAACAGCAGCGCGGGGAGCTGATCTCCTGCTTCCTGCTG
CGTATTGAGGATAATATGGAGTCCATCGGCCGGGCGGTGAACTCGGCGCTACAGCTGTCGAAGCGCGGCGGCGGGGTGGC
CTTTTTACTCTCCAACCTGCGCGAGGCCGGGGCGCCGATCAAGCGCATCGAAAACCAGTCTTCTGGGGTGGTGCCGGTGA
TGAAGATGCTGGAGGACGCCTTCTCCTACGCCAATCAGCTCGGCGCCCGCCAGGGGGCGGGTGCGGTCTGGCTGCATGTT
CACCATCCCGATATTTTACGCTTTCTTGATACTCGGCGTGAAAATGCCGATGAGAAAATCCGCATCAAAACCTTGTCGCT
GGGGGTGGTGATCCCTGATATCACCTTCCAGCTGGCGAAAGAAGATGCGCAGATGGCCCTGTTCTCGCCTTATGACGTCG
AACGGCTGTACGGCAAACCTTTCGCCGATTGCGCCATCGGCGACCTCTATCCGCAGCTGGTGGCCGACGAGCGGGTCCGC
AAGCGCTGGATCCGCGCCCGCGACCTGTTCCAGCGCCTGGCGGAGATCCAGTTTGAATCCGGCTACCCGTACATCATGTT
TGAAGACACCGTGAACCGCGCCAGTCCGGTGGCCGGGCGGGTCACCATGAGCAATCTGTGTTCGGAGATCCTGCAGGTCA
GTACGCCGTCCGCCTATAATGAAGACCTCAGCTATGCCCATATCGGGGAAGATATCTCCTGCAACCTCGGCTCGCTGAAC
ATCGCCCACACCATGGACTCGCCGGATTTCGGCCGCACAATCGCCACCGCGGTGCGCGCCCTGACCGCCGTGTCCGACAT
GAGCCACATTCAGTCGGTTCCCTCGGTGGCGGCCGGCAACGCGGCCTCCCACGCCATCGGCCTGGGGCAGATGAATCTGC
ACGGTTACCTGGCGCGGGAGGGCATTGCCTACGGCAGCCCGGAGGGGCTGGACTTCACCAATATCTACTTCTACACCGTG
ACCTGGCACGCGCTGCACACCTCGATGCAGCTGGCCCGCGAGCGCGGCCAGCGCTTCGCCGGCTTCGAGCAGTCGCGCTA
CGCCAGCGGGGCGTACTTCGACAAATATCTGCAGGAGGAGTGGCAGCCGAAAACCGAACGGGTGCGCACGCTGTTCGCCC
GCGCCGGCATCTCTCTGCCCGACCGGGAAAGCTGGCGCCAGCTGCGCGACGATGTCATGCGCTATGGCATCTACAATCGC
TATCTGCAGGCGGTGCCGCCCACCGGCTCGATCTCGTACATCAACCATGCCACCTCCAGCATTCACCCGATCGTCTCGAA
GATTGAGATCCGCAAAGAGGGGAAAACCGGGCGGGTGTACTACCCCGCGCCATTTATGAATAACGACAATCTGGCCCTCT
ACCAGGATGCGTATGAGATAGGGCCGGAGAAGATCATTGACACTTACGCCGAGGCGACGCGCCACGTCGACCAGGGGCTG
TCGCTGACCCTGTTCTTCCCCGATACCGCCACCACCCGCGATATCAACAAGGCGCAAATTTACGCCTGGAAGAAAGGCAT
CAAGACGCTCTACTACATCCGTCTGCGCCAGCTGGCGCTGGAAGGCACTGAAATTGAAGGCTGCGTGTCCTGCGCGCTGT
AA

Upstream 100 bases:

>100_bases
CCGACGATATCAACACCGTGCGTAAAGGAGTCAGCGAATTTTGGCAACGACAACCGCAGAACGTATAACCGCCGCCGTGG
ATTTTCACGCCCTCAACGCG

Downstream 100 bases:

>100_bases
GGAGAACAGGATGACCCATTTAACGCGCGTCAGCGCAATCAACTGGAACCGCATTGATGACGATAAAGATCTTGAGGTCT
GGAACCGTCTCACCAGCAAC

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: R1E protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MLNLYDSEGRIPFEKDRQAVEAFMATQVQPNALTFPSQEDKLSWLVSEGYYDPQVLAGYDRGFVLALFAHARRAPFRFQT
FLGAWKFYTSYALKTFDGKHYLEDFAERSVMVALTLARGDEQQARQLTEEILSGRFQPATPTFLNAGKQQRGELISCFLL
RIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVVPVMKMLEDAFSYANQLGARQGAGAVWLHV
HHPDILRFLDTRRENADEKIRIKTLSLGVVIPDITFQLAKEDAQMALFSPYDVERLYGKPFADCAIGDLYPQLVADERVR
KRWIRARDLFQRLAEIQFESGYPYIMFEDTVNRASPVAGRVTMSNLCSEILQVSTPSAYNEDLSYAHIGEDISCNLGSLN
IAHTMDSPDFGRTIATAVRALTAVSDMSHIQSVPSVAAGNAASHAIGLGQMNLHGYLAREGIAYGSPEGLDFTNIYFYTV
TWHALHTSMQLARERGQRFAGFEQSRYASGAYFDKYLQEEWQPKTERVRTLFARAGISLPDRESWRQLRDDVMRYGIYNR
YLQAVPPTGSISYINHATSSIHPIVSKIEIRKEGKTGRVYYPAPFMNNDNLALYQDAYEIGPEKIIDTYAEATRHVDQGL
SLTLFFPDTATTRDINKAQIYAWKKGIKTLYYIRLRQLALEGTEIEGCVSCAL

Sequences:

>Translated_693_residues
MLNLYDSEGRIPFEKDRQAVEAFMATQVQPNALTFPSQEDKLSWLVSEGYYDPQVLAGYDRGFVLALFAHARRAPFRFQT
FLGAWKFYTSYALKTFDGKHYLEDFAERSVMVALTLARGDEQQARQLTEEILSGRFQPATPTFLNAGKQQRGELISCFLL
RIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVVPVMKMLEDAFSYANQLGARQGAGAVWLHV
HHPDILRFLDTRRENADEKIRIKTLSLGVVIPDITFQLAKEDAQMALFSPYDVERLYGKPFADCAIGDLYPQLVADERVR
KRWIRARDLFQRLAEIQFESGYPYIMFEDTVNRASPVAGRVTMSNLCSEILQVSTPSAYNEDLSYAHIGEDISCNLGSLN
IAHTMDSPDFGRTIATAVRALTAVSDMSHIQSVPSVAAGNAASHAIGLGQMNLHGYLAREGIAYGSPEGLDFTNIYFYTV
TWHALHTSMQLARERGQRFAGFEQSRYASGAYFDKYLQEEWQPKTERVRTLFARAGISLPDRESWRQLRDDVMRYGIYNR
YLQAVPPTGSISYINHATSSIHPIVSKIEIRKEGKTGRVYYPAPFMNNDNLALYQDAYEIGPEKIIDTYAEATRHVDQGL
SLTLFFPDTATTRDINKAQIYAWKKGIKTLYYIRLRQLALEGTEIEGCVSCAL
>Mature_693_residues
MLNLYDSEGRIPFEKDRQAVEAFMATQVQPNALTFPSQEDKLSWLVSEGYYDPQVLAGYDRGFVLALFAHARRAPFRFQT
FLGAWKFYTSYALKTFDGKHYLEDFAERSVMVALTLARGDEQQARQLTEEILSGRFQPATPTFLNAGKQQRGELISCFLL
RIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVVPVMKMLEDAFSYANQLGARQGAGAVWLHV
HHPDILRFLDTRRENADEKIRIKTLSLGVVIPDITFQLAKEDAQMALFSPYDVERLYGKPFADCAIGDLYPQLVADERVR
KRWIRARDLFQRLAEIQFESGYPYIMFEDTVNRASPVAGRVTMSNLCSEILQVSTPSAYNEDLSYAHIGEDISCNLGSLN
IAHTMDSPDFGRTIATAVRALTAVSDMSHIQSVPSVAAGNAASHAIGLGQMNLHGYLAREGIAYGSPEGLDFTNIYFYTV
TWHALHTSMQLARERGQRFAGFEQSRYASGAYFDKYLQEEWQPKTERVRTLFARAGISLPDRESWRQLRDDVMRYGIYNR
YLQAVPPTGSISYINHATSSIHPIVSKIEIRKEGKTGRVYYPAPFMNNDNLALYQDAYEIGPEKIIDTYAEATRHVDQGL
SLTLFFPDTATTRDINKAQIYAWKKGIKTLYYIRLRQLALEGTEIEGCVSCAL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1E contains the binding sites for both substrates and allosteric effectors and carries out the actual reductio

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=643, Percent_Identity=26.7496111975117, Blast_Score=191, Evalue=2e-48,
Organism=Escherichia coli, GI1789030, Length=693, Percent_Identity=83.2611832611833, Blast_Score=1225, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=581, Percent_Identity=25.3012048192771, Blast_Score=159, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17555242, Length=640, Percent_Identity=26.5625, Blast_Score=190, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322123, Length=644, Percent_Identity=26.3975155279503, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320914, Length=645, Percent_Identity=26.5116279069767, Blast_Score=183, Evalue=8e-47,
Organism=Drosophila melanogaster, GI17352481, Length=643, Percent_Identity=24.4167962674961, Blast_Score=162, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 78085; Mature: 78085

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLYDSEGRIPFEKDRQAVEAFMATQVQPNALTFPSQEDKLSWLVSEGYYDPQVLAGYD
CCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEECCCCCCCHHEECCC
RGFVLALFAHARRAPFRFQTFLGAWKFYTSYALKTFDGKHYLEDFAERSVMVALTLARGD
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEECCCC
EQQARQLTEEILSGRFQPATPTFLNAGKQQRGELISCFLLRIEDNMESIGRAVNSALQLS
HHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRGGGVAFLLSNLREAGAPIKRIENQSSGVVPVMKMLEDAFSYANQLGARQGAGAVWLHV
HCCCCHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
HHPDILRFLDTRRENADEKIRIKTLSLGVVIPDITFQLAKEDAQMALFSPYDVERLYGKP
CCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCHHHHHHHCCCCEEEECCCCHHHHCCCC
FADCAIGDLYPQLVADERVRKRWIRARDLFQRLAEIQFESGYPYIMFEDTVNRASPVAGR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCEEEEECCCCCCCCCCCH
VTMSNLCSEILQVSTPSAYNEDLSYAHIGEDISCNLGSLNIAHTMDSPDFGRTIATAVRA
HHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
LTAVSDMSHIQSVPSVAAGNAASHAIGLGQMNLHGYLAREGIAYGSPEGLDFTNIYFYTV
HHHHHHHHHHHHCCCHHCCCCHHHHEECCCCCHHHHHHHCCCCCCCCCCCCEEEEEEEEE
TWHALHTSMQLARERGQRFAGFEQSRYASGAYFDKYLQEEWQPKTERVRTLFARAGISLP
EHHHHHHHHHHHHHHCHHHCCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
DRESWRQLRDDVMRYGIYNRYLQAVPPTGSISYINHATSSIHPIVSKIEIRKEGKTGRVY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEE
YPAPFMNNDNLALYQDAYEIGPEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQI
ECCCCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHH
YAWKKGIKTLYYIRLRQLALEGTEIEGCVSCAL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure
MLNLYDSEGRIPFEKDRQAVEAFMATQVQPNALTFPSQEDKLSWLVSEGYYDPQVLAGYD
CCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEECCCCCCCHHEECCC
RGFVLALFAHARRAPFRFQTFLGAWKFYTSYALKTFDGKHYLEDFAERSVMVALTLARGD
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEECCCC
EQQARQLTEEILSGRFQPATPTFLNAGKQQRGELISCFLLRIEDNMESIGRAVNSALQLS
HHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRGGGVAFLLSNLREAGAPIKRIENQSSGVVPVMKMLEDAFSYANQLGARQGAGAVWLHV
HCCCCHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
HHPDILRFLDTRRENADEKIRIKTLSLGVVIPDITFQLAKEDAQMALFSPYDVERLYGKP
CCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCHHHHHHHCCCCEEEECCCCHHHHCCCC
FADCAIGDLYPQLVADERVRKRWIRARDLFQRLAEIQFESGYPYIMFEDTVNRASPVAGR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCEEEEECCCCCCCCCCCH
VTMSNLCSEILQVSTPSAYNEDLSYAHIGEDISCNLGSLNIAHTMDSPDFGRTIATAVRA
HHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
LTAVSDMSHIQSVPSVAAGNAASHAIGLGQMNLHGYLAREGIAYGSPEGLDFTNIYFYTV
HHHHHHHHHHHHCCCHHCCCCHHHHEECCCCCHHHHHHHCCCCCCCCCCCCEEEEEEEEE
TWHALHTSMQLARERGQRFAGFEQSRYASGAYFDKYLQEEWQPKTERVRTLFARAGISLP
EHHHHHHHHHHHHHHCHHHCCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
DRESWRQLRDDVMRYGIYNRYLQAVPPTGSISYINHATSSIHPIVSKIEIRKEGKTGRVY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEE
YPAPFMNNDNLALYQDAYEIGPEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQI
ECCCCCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHH
YAWKKGIKTLYYIRLRQLALEGTEIEGCVSCAL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8820648; 2158980 [H]