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The map label for this gene is srlB [H]

Identifier: 152971479

GI number: 152971479

Start: 3243977

End: 3244351

Strand: Direct

Name: srlB [H]

Synonym: KPN_02952

Alternate gene names: 152971479

Gene position: 3243977-3244351 (Clockwise)

Preceding gene: 152971478

Following gene: 152971480

Centisome position: 61.03

GC content: 60.8

Gene sequence:

>375_bases
ATGATGATTTACTGCGCGCGTATCGTCGCCATCGGGCTGTTTGTCGCCGACGGCCTGACCGACAAGATGCTCATCACCTT
CGACAGCAACGGGCCGAAGGACTGTCTCGATTACTCCCTGTCGCTGGAGCCGTCGTTCCGGGAAGAGAGCCTGATGATAC
TCCCCGGCGACCGCCTGCTGCTGGCGGGCCATGATTATCTGGTGACCAGTGTTGGCAAAGGGGCACAACAGGCGCTGTTT
GAACTCGGCCACCTGACGCTGGTGTTTAACGGCGACCTGAATGCCTGCCATGTCGGCGCGGTTCATCTCTCCGGCCCGGT
GCCGAAACTCCGCGACCTGCACGGCAATCTGGTCATTGAGGAGGGACGCCCATGA

Upstream 100 bases:

>100_bases
TGGCGTTGCGCTATATCGCTGCCGCCATGCTGATTGGTTTAATTCGCGGGATTATCACCGAGTACCTGTTCCTGCGGCTC
AGCAGGCGGGGGAATGCGCC

Downstream 100 bases:

>100_bases
ACACGGTGCTTATTCCCCCCGGCCCCGGTGGCTACGGCAAGGGACTGTGGCTGCCCGTCGGCAGCGGCAAAAAGGTGCTG
TCGCTCACCGGGCGGGAAAT

Product: putative glucitol-specific PTS enzyme III

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Gut; EIII-Gut; PTS system glucitol/sorbitol-specific EIIA component [H]

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MMIYCARIVAIGLFVADGLTDKMLITFDSNGPKDCLDYSLSLEPSFREESLMILPGDRLLLAGHDYLVTSVGKGAQQALF
ELGHLTLVFNGDLNACHVGAVHLSGPVPKLRDLHGNLVIEEGRP

Sequences:

>Translated_124_residues
MMIYCARIVAIGLFVADGLTDKMLITFDSNGPKDCLDYSLSLEPSFREESLMILPGDRLLLAGHDYLVTSVGKGAQQALF
ELGHLTLVFNGDLNACHVGAVHLSGPVPKLRDLHGNLVIEEGRP
>Mature_124_residues
MMIYCARIVAIGLFVADGLTDKMLITFDSNGPKDCLDYSLSLEPSFREESLMILPGDRLLLAGHDYLVTSVGKGAQQALF
ELGHLTLVFNGDLNACHVGAVHLSGPVPKLRDLHGNLVIEEGRP

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3731

COG function: function code G; Phosphotransferase system sorbitol-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI1789056, Length=107, Percent_Identity=41.1214953271028, Blast_Score=70, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004716
- InterPro:   IPR018454
- ProDom:   PD015842 [H]

Pfam domain/function: PF03829 PTSIIA_gutA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 13435; Mature: 13435

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS51097 PTS_EIIA_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIYCARIVAIGLFVADGLTDKMLITFDSNGPKDCLDYSLSLEPSFREESLMILPGDRLL
CEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHCEEEECCCCCCCCEEEEECCCEEE
LAGHDYLVTSVGKGAQQALFELGHLTLVFNGDLNACHVGAVHLSGPVPKLRDLHGNLVIE
EECCCEEEEECCCCHHHHHHHCCCEEEEEECCCCEEEEEEEEECCCCCHHHCCCCCEEEE
EGRP
CCCC
>Mature Secondary Structure
MMIYCARIVAIGLFVADGLTDKMLITFDSNGPKDCLDYSLSLEPSFREESLMILPGDRLL
CEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHCEEEECCCCCCCCEEEEECCCEEE
LAGHDYLVTSVGKGAQQALFELGHLTLVFNGDLNACHVGAVHLSGPVPKLRDLHGNLVIE
EECCCEEEEECCCCHHHHHHHCCCEEEEEECCCCEEEEEEEEECCCCCHHHCCCCCEEEE
EGRP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferases; Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3553176; 1630305; 9205837; 9278503 [H]