Click here to switch to the map view.

The map label for this gene is sseA [H]

Identifier: 152971382

GI number: 152971382

Start: 3134905

End: 3135762

Strand: Direct

Name: sseA [H]

Synonym: KPN_02851

Alternate gene names: 152971382

Gene position: 3134905-3135762 (Clockwise)

Preceding gene: 152971366

Following gene: 152971383

Centisome position: 58.98

GC content: 63.05

Gene sequence:

>858_bases
ATGTCCACCTCGTTTTTTGTCGCTGCCGACTGGCTGGCAGAACATATTGATGACCCGGAAATTCAGATTATCGACGCCCG
CATGGCGCCCGCCGGGCAGGAGGCCCTGCGGGATATGGCCGCGGAATACCGCGCCGGCCATGTGCCGAACGCGCTGTTTT
TCGATATTGAGGCGCTATCCGACCACACCAGTCCGCTGCCGCATATGATGCCGCGGGCCGAAGCGTTTGCCGTCGCCATG
CGCGAACTGGGCGTCTGCAGCGATAAGCATCTGGTGGTCTATGACGAAGGCAATCTGTTTTCCGCCCCGCGCGCGTGGTG
GATGCTGCGCACCTTTGGCGTAGAGAAGGTGTCAATTCTGGCCGGCGGGCTGGAGGGCTGGCGGCGCGACGAACTGCCGC
TGGAACAAGGGATGCCGGAAGTGGCCGAAGGGGAATTCGACGTGCGGTTCGATCCTCAACAAATTAAGCGTCTGACCGAC
GTCCTGTTGGTCAGCCATGAAGGATCGGCGCAGATTGTCGATGCCCGTCCGGCGGCCCGGTTTAACGGCCAGGCCGACGA
ACCGCGCCCGGGCCTGCGCCGCGGGCATATCCCCGGCGCGCTGAACGTACCGTGGACGGATCTGGTGATTAACGGCGAAC
TGAAAACCGTTGACGAGCTGAACGACATCTTTTTACGTCAGGGTGTCGATTTTGAACGGCCGATCATCGCCAGCTGCGGC
TCGGGGGTGACGGCGGCGGTGGTCGTGCTGGCGCTCACCACGCTTGGCGTGAACGGCGTCTGCCTGTATGACGGCTCCTG
GAGCGAATGGGGCGCGCGGAGCGATCTGCCGATTGAACCGGCCCCTGCCGCTCCGTAA

Upstream 100 bases:

>100_bases
GAAAAGTGCCGTGACGCCGTCCCCACTCTGGCGTCCTTAACTTGTCAGCGCCAGCTAAAACGCCGACAATTCAGTCATGA
ATAATAAAACGGAGGCACCT

Downstream 100 bases:

>100_bases
ACACAGCGTGACCGCGGGCCTCCCGTTCCGCGGTCACTAAATAATTTCTGATTTTCTCTCCCACTTTCTTCTGCTTCTCG
CTAAACCGGTTACTCTGTGC

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSTSFFVAADWLAEHIDDPEIQIIDARMAPAGQEALRDMAAEYRAGHVPNALFFDIEALSDHTSPLPHMMPRAEAFAVAM
RELGVCSDKHLVVYDEGNLFSAPRAWWMLRTFGVEKVSILAGGLEGWRRDELPLEQGMPEVAEGEFDVRFDPQQIKRLTD
VLLVSHEGSAQIVDARPAARFNGQADEPRPGLRRGHIPGALNVPWTDLVINGELKTVDELNDIFLRQGVDFERPIIASCG
SGVTAAVVVLALTTLGVNGVCLYDGSWSEWGARSDLPIEPAPAAP

Sequences:

>Translated_285_residues
MSTSFFVAADWLAEHIDDPEIQIIDARMAPAGQEALRDMAAEYRAGHVPNALFFDIEALSDHTSPLPHMMPRAEAFAVAM
RELGVCSDKHLVVYDEGNLFSAPRAWWMLRTFGVEKVSILAGGLEGWRRDELPLEQGMPEVAEGEFDVRFDPQQIKRLTD
VLLVSHEGSAQIVDARPAARFNGQADEPRPGLRRGHIPGALNVPWTDLVINGELKTVDELNDIFLRQGVDFERPIIASCG
SGVTAAVVVLALTTLGVNGVCLYDGSWSEWGARSDLPIEPAPAAP
>Mature_284_residues
STSFFVAADWLAEHIDDPEIQIIDARMAPAGQEALRDMAAEYRAGHVPNALFFDIEALSDHTSPLPHMMPRAEAFAVAMR
ELGVCSDKHLVVYDEGNLFSAPRAWWMLRTFGVEKVSILAGGLEGWRRDELPLEQGMPEVAEGEFDVRFDPQQIKRLTDV
LLVSHEGSAQIVDARPAARFNGQADEPRPGLRRGHIPGALNVPWTDLVINGELKTVDELNDIFLRQGVDFERPIIASCGS
GVTAAVVVLALTTLGVNGVCLYDGSWSEWGARSDLPIEPAPAAP

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=278, Percent_Identity=42.4460431654676, Blast_Score=212, Evalue=3e-55,
Organism=Homo sapiens, GI61835204, Length=278, Percent_Identity=42.0863309352518, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI194473681, Length=278, Percent_Identity=42.0863309352518, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI17402865, Length=279, Percent_Identity=39.7849462365591, Blast_Score=174, Evalue=6e-44,
Organism=Escherichia coli, GI87082121, Length=280, Percent_Identity=74.6428571428571, Blast_Score=433, Evalue=1e-123,
Organism=Escherichia coli, GI87081967, Length=256, Percent_Identity=23.828125, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI115534702, Length=209, Percent_Identity=33.4928229665072, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71997283, Length=299, Percent_Identity=22.4080267558528, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17561888, Length=296, Percent_Identity=23.3108108108108, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17559150, Length=303, Percent_Identity=23.1023102310231, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543836, Length=242, Percent_Identity=23.5537190082645, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17543838, Length=242, Percent_Identity=23.5537190082645, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324825, Length=249, Percent_Identity=35.7429718875502, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 31178; Mature: 31046

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSFFVAADWLAEHIDDPEIQIIDARMAPAGQEALRDMAAEYRAGHVPNALFFDIEALS
CCCCEEEHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHC
DHTSPLPHMMPRAEAFAVAMRELGVCSDKHLVVYDEGNLFSAPRAWWMLRTFGVEKVSIL
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEECCCHHHHHHHHHCCCEEEEE
AGGLEGWRRDELPLEQGMPEVAEGEFDVRFDPQQIKRLTDVLLVSHEGSAQIVDARPAAR
CCCCCCCCCCCCCHHHCCCHHHCCCEEEEECHHHHHHHHHHEEEECCCCEEEEECCCCHH
FNGQADEPRPGLRRGHIPGALNVPWTDLVINGELKTVDELNDIFLRQGVDFERPIIASCG
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHCC
SGVTAAVVVLALTTLGVNGVCLYDGSWSEWGARSDLPIEPAPAAP
CCHHHHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STSFFVAADWLAEHIDDPEIQIIDARMAPAGQEALRDMAAEYRAGHVPNALFFDIEALS
CCCEEEHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHC
DHTSPLPHMMPRAEAFAVAMRELGVCSDKHLVVYDEGNLFSAPRAWWMLRTFGVEKVSIL
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEECCCHHHHHHHHHCCCEEEEE
AGGLEGWRRDELPLEQGMPEVAEGEFDVRFDPQQIKRLTDVLLVSHEGSAQIVDARPAAR
CCCCCCCCCCCCCHHHCCCHHHCCCEEEEECHHHHHHHHHHEEEECCCCEEEEECCCCHH
FNGQADEPRPGLRRGHIPGALNVPWTDLVINGELKTVDELNDIFLRQGVDFERPIIASCG
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHCC
SGVTAAVVVLALTTLGVNGVCLYDGSWSEWGARSDLPIEPAPAAP
CCHHHHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]