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The map label for this gene is purN [H]

Identifier: 152971356

GI number: 152971356

Start: 3096402

End: 3097043

Strand: Direct

Name: purN [H]

Synonym: KPN_02825

Alternate gene names: 152971356

Gene position: 3096402-3097043 (Clockwise)

Preceding gene: 152971355

Following gene: 152971357

Centisome position: 58.26

GC content: 60.9

Gene sequence:

>642_bases
ATGAAAAACATCGTGGTGCTGATTTCCGGTAGCGGCAGCAATTTGCAGGCGATCATCGACGCCTGCGGCCGGAAACAGAT
AAACGGCACCCTGCGTGCGGTATTCAGTAACAAGGCTGATGCGTTCGGCCTTGAGCGCGCGCGCCTGGCCGGCATCCCGG
CCCATGCGCTGGCGCAAAGTCAGTTTGCCGACCGGGAAGCGTTCGATCGTCAGCTGATGCATGAAATCGACGCCTACGCC
CCGGATCTGGTGGTGCTGGCAGGCTATATGCGTATCCTTAGCCCGGCGTTCGTCAGCCACTATCAGGGACGTCTGCTGAA
CATCCATCCCTCGCTACTGCCGAAGTATCCTGGCCTGCATACTCATCGTCAGGTGCTGGAGAACGGTGACGAGGAGCACG
GTACCTCGGTGCACTTCGTCACCGATGAGCTGGACGGTGGGCCGGTTATCCTGCAGGCCAAGGTGCCGGTCTTCGCTGGC
GACAGCGAGGAGGAAATCACGGCGCGGGTTCAGGCCCAGGAACATGCCATTTATCCGCTGGTCATCAGCTGGTTCGTCGA
TGGACGTCTGCGCATGGCAGGCAACCACGCCTGGCTGGATGAGCGACAGCTTCCCCCGCAGGGCTATGCCGCCGACGAAT
GA

Upstream 100 bases:

>100_bases
AAGCGGACAAAGCGATTGCCCTGCTGAATGACAAAGGTGAAAACGCGTGGAAAATCGGGTATATCAAAGCCTCCGATTCC
GAACAGCGTGTGGTCATTGC

Downstream 100 bases:

>100_bases
ACAACCAGCCCCGCATGAGCACATCGCGGGGCTTTTTTTTAATTAAAAGTGTTTACTAATCAAACATCTCACAGAAATAA
TTCTTCTTACTGTCATATTG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKNIVVLISGSGSNLQAIIDACGRKQINGTLRAVFSNKADAFGLERARLAGIPAHALAQSQFADREAFDRQLMHEIDAYA
PDLVVLAGYMRILSPAFVSHYQGRLLNIHPSLLPKYPGLHTHRQVLENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAG
DSEEEITARVQAQEHAIYPLVISWFVDGRLRMAGNHAWLDERQLPPQGYAADE

Sequences:

>Translated_213_residues
MKNIVVLISGSGSNLQAIIDACGRKQINGTLRAVFSNKADAFGLERARLAGIPAHALAQSQFADREAFDRQLMHEIDAYA
PDLVVLAGYMRILSPAFVSHYQGRLLNIHPSLLPKYPGLHTHRQVLENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAG
DSEEEITARVQAQEHAIYPLVISWFVDGRLRMAGNHAWLDERQLPPQGYAADE
>Mature_213_residues
MKNIVVLISGSGSNLQAIIDACGRKQINGTLRAVFSNKADAFGLERARLAGIPAHALAQSQFADREAFDRQLMHEIDAYA
PDLVVLAGYMRILSPAFVSHYQGRLLNIHPSLLPKYPGLHTHRQVLENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAG
DSEEEITARVQAQEHAIYPLVISWFVDGRLRMAGNHAWLDERQLPPQGYAADE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=201, Percent_Identity=40.2985074626866, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI209869995, Length=201, Percent_Identity=40.2985074626866, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI209869993, Length=201, Percent_Identity=40.2985074626866, Blast_Score=163, Evalue=1e-40,
Organism=Escherichia coli, GI1788846, Length=211, Percent_Identity=83.4123222748815, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1787483, Length=93, Percent_Identity=43.010752688172, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=176, Percent_Identity=38.6363636363636, Blast_Score=126, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=32.1608040201005, Blast_Score=85, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=47.0899470899471, Blast_Score=184, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23455; Mature: 23455

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIVVLISGSGSNLQAIIDACGRKQINGTLRAVFSNKADAFGLERARLAGIPAHALAQS
CCCEEEEEECCCCCHHHHHHHHCCHHHCHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHH
QFADREAFDRQLMHEIDAYAPDLVVLAGYMRILSPAFVSHYQGRLLNIHPSLLPKYPGLH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCCH
THRQVLENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGDSEEEITARVQAQEHAIYPL
HHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEECCEEECCCHHHHHHHHHHHHCCHHHH
VISWFVDGRLRMAGNHAWLDERQLPPQGYAADE
HEEEHHCCEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKNIVVLISGSGSNLQAIIDACGRKQINGTLRAVFSNKADAFGLERARLAGIPAHALAQS
CCCEEEEEECCCCCHHHHHHHHCCHHHCHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHH
QFADREAFDRQLMHEIDAYAPDLVVLAGYMRILSPAFVSHYQGRLLNIHPSLLPKYPGLH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHCCCCCCCH
THRQVLENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGDSEEEITARVQAQEHAIYPL
HHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEECCEEECCCHHHHHHHHHHHHCCHHHH
VISWFVDGRLRMAGNHAWLDERQLPPQGYAADE
HEEEHHCCEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]