Click here to switch to the map view.

The map label for this gene is mnmC

Identifier: 152971252

GI number: 152971252

Start: 2984432

End: 2986420

Strand: Direct

Name: mnmC

Synonym: KPN_02714

Alternate gene names: 152971252

Gene position: 2984432-2986420 (Clockwise)

Preceding gene: 152971249

Following gene: 152971259

Centisome position: 56.15

GC content: 63.05

Gene sequence:

>1989_bases
GTGAAACAAAACGCCATACAACCCGCCAACCTGGAATTCAACGCTGAGGGTACACCTGTTTCCCGAGATTTCGACGATGT
TTACTTTTCCAATGACAACGGTCTGGAAGAGACTCGCTACGTTTTTCTCGGCGGAAATCGCCTGCCGGAGCGCTTCCCCA
GCCACCCTCGCCCGCTGATGATCGTCGCGGAGAGCGGCTTCGGCACCGGGCTCAATTTCCTCACCCTCTGGCAGGCATTT
GACGTTTTTGTTCGTGATAACCCGAACGTTACCTTGCAAAGGTTACATTTCATCAGTTTTGAAAAATATCCGCTGAAGGC
TGAGGATCTCCGCCTGGCGCACCAGCGCTGGCCAGAGCTGGCTCCGTGGGCGCAGCAGCTGCAGGCGCAATGGCCGTCGG
CCTTCGGCGGGTGCCATCGTCTGCTGCTCGACGGCGGGCGGGTGACGCTCGATCTGTGGTTTGGCGATATCAATGAGCTG
ACCAGGGAGCTGGATGATTCGCTGAACCAGCAGGTGGACGCCTGGTTTCTTGACGGCTTTGCCCCGGCGAAAAATCCCGA
CATGTGGACTCAGGATCTGTTCAGCGCCATGGCGCGCCTGGCGCGACCCGGCGGGACGCTGGCCACCTTTACCTCCGCCG
GTTTTGTCCGCCGTGGCCTGCAGGAGGCCGGCTTTACCATGCGTAAAAGCAAAGGCTTCGGCCGCAAGCGGGAGATGCTG
ACCGGCGAGATGGCGCAAACGCTGAGCTTTCCTGCGCGCGTCCCCTGGTTCGCTCGCAGTAGCAGCGATGCCCGCGAGGC
GGCCATCATCGGCGGCGGGATCGCCAGCGCCCTGCTCTCCCTGGCGCTGCTGCGCCGCGGCTGGCAGGTGACCCTTTACT
GCGCCGATGAGGCTCCAGCGCAGGGCGCCTCCGGCAATCGCCAGGGCGCGCTGTATCCGCTGTTAAGTCAGCACGATCCG
GCGCTGGCACGCTTTTTTCCGGCCGCCTTTACCTTCGCCCGCCGAATGTATGACGCGCTGCCGGTGATGTTCGATCACCA
ATGGTGCGGTGTTACCCAGCTCGGCTGGGATGAGAAAAGCACGCATAAAATCGCCCAGATGCTGGCGCTGAATTTACCCC
CGGACATCGCCTGCGCGGTGACGGCGGAACAGGTTGCCGGGCTGACCGGCGTCGATACCGGCTGCGGCGGCATCACCTAT
CCGGCCGGCGGCTGGCTGTGCCCGCAGCAGCTCACCGCCGAGCTGCTGGCGCTGGCGGCCACGCGCGGACTGCACGTGCA
CTATGGTTATCCCGTCGAAACGCTCAGCGCGGAGGGCGATGGCTGGCTGCTCAATCAGCAGCGCTATCATCAGGCGGTGG
TTCTGGCGAACGGCCACCGCATCACTGGTTTTGCGCAAACCGCCCAACTGCCGGTCTACCCGGTTGGCGGCCAGGTCAGC
CATATTCCCACCACCCCGCGGCTTGCCGCCCTGCGCCAGGTGCTGTGCTACGACGGCTACCTGACGCCACAGAATCCGCA
AAATCAGCAGCACTGCATTGGCGCCAGCTACCACCGCGGGAATACCGACACCACCTTCAGCGAAGAAGATCAGCAGCACA
ACCGGCAACGGCTGATCGACTGCTTCCCGGGCGCGGAGTGGCCACAGGACGTGGATATCAGCGCCAACGACGCTCGCTGC
GGCGTGCGCTGCGCCACCCGCGATCATCTACCGATGGTCGGCAACGTGCCGGACTACGCGGCAACATTGACGCAATACGC
CAGCCTCCACGAACAACCGGATATCGCCGATAGCGCGCCGGTCTGTCGTAACCTGTTTATGCTTGGCGCGCTCGGGTCGC
GCGGACTTTGCACCGCGCCGTTAAGCGCTGAGCTGCTGGCGGCGCAAATGAGCGCGGAACCGCTGCCGCTGGATAGCGAC
ACCCTGGCGGCGCTGAATCCGAATCGGCTGTGGGTGAGAAAACTGCTGAAGGGAAAAGCGGTGAAATAA

Upstream 100 bases:

>100_bases
GTACGCCGAACAAGTCCGATCAGACATTTTGTGTATAAATTTGCGCCCTCAGGCACACATCGTTAAGATCGTGCTACGCC
TTGTGATTCGAGTAACTTAC

Downstream 100 bases:

>100_bases
AACCATGAGGGCAGGCAATTTCCCGGGTACGGCTGACGCCTGACCCGGGCTACAACATGGCTCAGGCCTGATTTTTCGCC
TGTTGATACAGGTTATGCCA

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MKQNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLPERFPSHPRPLMIVAESGFGTGLNFLTLWQAF
DVFVRDNPNVTLQRLHFISFEKYPLKAEDLRLAHQRWPELAPWAQQLQAQWPSAFGGCHRLLLDGGRVTLDLWFGDINEL
TRELDDSLNQQVDAWFLDGFAPAKNPDMWTQDLFSAMARLARPGGTLATFTSAGFVRRGLQEAGFTMRKSKGFGRKREML
TGEMAQTLSFPARVPWFARSSSDAREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPAQGASGNRQGALYPLLSQHDP
ALARFFPAAFTFARRMYDALPVMFDHQWCGVTQLGWDEKSTHKIAQMLALNLPPDIACAVTAEQVAGLTGVDTGCGGITY
PAGGWLCPQQLTAELLALAATRGLHVHYGYPVETLSAEGDGWLLNQQRYHQAVVLANGHRITGFAQTAQLPVYPVGGQVS
HIPTTPRLAALRQVLCYDGYLTPQNPQNQQHCIGASYHRGNTDTTFSEEDQQHNRQRLIDCFPGAEWPQDVDISANDARC
GVRCATRDHLPMVGNVPDYAATLTQYASLHEQPDIADSAPVCRNLFMLGALGSRGLCTAPLSAELLAAQMSAEPLPLDSD
TLAALNPNRLWVRKLLKGKAVK

Sequences:

>Translated_662_residues
MKQNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLPERFPSHPRPLMIVAESGFGTGLNFLTLWQAF
DVFVRDNPNVTLQRLHFISFEKYPLKAEDLRLAHQRWPELAPWAQQLQAQWPSAFGGCHRLLLDGGRVTLDLWFGDINEL
TRELDDSLNQQVDAWFLDGFAPAKNPDMWTQDLFSAMARLARPGGTLATFTSAGFVRRGLQEAGFTMRKSKGFGRKREML
TGEMAQTLSFPARVPWFARSSSDAREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPAQGASGNRQGALYPLLSQHDP
ALARFFPAAFTFARRMYDALPVMFDHQWCGVTQLGWDEKSTHKIAQMLALNLPPDIACAVTAEQVAGLTGVDTGCGGITY
PAGGWLCPQQLTAELLALAATRGLHVHYGYPVETLSAEGDGWLLNQQRYHQAVVLANGHRITGFAQTAQLPVYPVGGQVS
HIPTTPRLAALRQVLCYDGYLTPQNPQNQQHCIGASYHRGNTDTTFSEEDQQHNRQRLIDCFPGAEWPQDVDISANDARC
GVRCATRDHLPMVGNVPDYAATLTQYASLHEQPDIADSAPVCRNLFMLGALGSRGLCTAPLSAELLAAQMSAEPLPLDSD
TLAALNPNRLWVRKLLKGKAVK
>Mature_662_residues
MKQNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLPERFPSHPRPLMIVAESGFGTGLNFLTLWQAF
DVFVRDNPNVTLQRLHFISFEKYPLKAEDLRLAHQRWPELAPWAQQLQAQWPSAFGGCHRLLLDGGRVTLDLWFGDINEL
TRELDDSLNQQVDAWFLDGFAPAKNPDMWTQDLFSAMARLARPGGTLATFTSAGFVRRGLQEAGFTMRKSKGFGRKREML
TGEMAQTLSFPARVPWFARSSSDAREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPAQGASGNRQGALYPLLSQHDP
ALARFFPAAFTFARRMYDALPVMFDHQWCGVTQLGWDEKSTHKIAQMLALNLPPDIACAVTAEQVAGLTGVDTGCGGITY
PAGGWLCPQQLTAELLALAATRGLHVHYGYPVETLSAEGDGWLLNQQRYHQAVVLANGHRITGFAQTAQLPVYPVGGQVS
HIPTTPRLAALRQVLCYDGYLTPQNPQNQQHCIGASYHRGNTDTTFSEEDQQHNRQRLIDCFPGAEWPQDVDISANDARC
GVRCATRDHLPMVGNVPDYAATLTQYASLHEQPDIADSAPVCRNLFMLGALGSRGLCTAPLSAELLAAQMSAEPLPLDSD
TLAALNPNRLWVRKLLKGKAVK

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=666, Percent_Identity=76.5765765765766, Blast_Score=1039, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_KLEP7 (A6TC10)

Other databases:

- EMBL:   CP000647
- RefSeq:   YP_001336361.1
- ProteinModelPortal:   A6TC10
- SMR:   A6TC10
- STRING:   A6TC10
- GeneID:   5340546
- GenomeReviews:   CP000647_GR
- KEGG:   kpn:KPN_02714
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- ProtClustDB:   PRK01747
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 72905; Mature: 72905

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLPERFPSHPRPLM
CCCCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCHHHCCCCCCCEE
IVAESGFGTGLNFLTLWQAFDVFVRDNPNVTLQRLHFISFEKYPLKAEDLRLAHQRWPEL
EEEECCCCCCHHHHHHHHHHEEEEECCCCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCC
APWAQQLQAQWPSAFGGCHRLLLDGGRVTLDLWFGDINELTRELDDSLNQQVDAWFLDGF
CHHHHHHHHHCCHHHHHHHHHEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHEECCC
APAKNPDMWTQDLFSAMARLARPGGTLATFTSAGFVRRGLQEAGFTMRKSKGFGRKREML
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCEEECCCCCCHHHHHH
TGEMAQTLSFPARVPWFARSSSDAREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
HHHHHHHHCCCCCCCEEECCCCCCHHHEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
QGASGNRQGALYPLLSQHDPALARFFPAAFTFARRMYDALPVMFDHQWCGVTQLGWDEKS
CCCCCCCCCCEECHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHH
THKIAQMLALNLPPDIACAVTAEQVAGLTGVDTGCGGITYPAGGWLCPQQLTAELLALAA
HHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TRGLHVHYGYPVETLSAEGDGWLLNQQRYHQAVVLANGHRITGFAQTAQLPVYPVGGQVS
HCCEEEEECCCHHEECCCCCCEEECHHHHCEEEEEECCCEEEEEHHHCCCCEEECCCCEE
HIPTTPRLAALRQVLCYDGYLTPQNPQNQQHCIGASYHRGNTDTTFSEEDQQHNRQRLID
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
CFPGAEWPQDVDISANDARCGVRCATRDHLPMVGNVPDYAATLTQYASLHEQPDIADSAP
HCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCH
VCRNLFMLGALGSRGLCTAPLSAELLAAQMSAEPLPLDSDTLAALNPNRLWVRKLLKGKA
HHHHHHHHHCCCCCCCEECCCHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHCCCC
VK
CC
>Mature Secondary Structure
MKQNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLPERFPSHPRPLM
CCCCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCHHHCCCCCCCEE
IVAESGFGTGLNFLTLWQAFDVFVRDNPNVTLQRLHFISFEKYPLKAEDLRLAHQRWPEL
EEEECCCCCCHHHHHHHHHHEEEEECCCCEEEEEEEEEEECCCCCCHHHHHHHHHHCCCC
APWAQQLQAQWPSAFGGCHRLLLDGGRVTLDLWFGDINELTRELDDSLNQQVDAWFLDGF
CHHHHHHHHHCCHHHHHHHHHEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHEECCC
APAKNPDMWTQDLFSAMARLARPGGTLATFTSAGFVRRGLQEAGFTMRKSKGFGRKREML
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCEEECCCCCCHHHHHH
TGEMAQTLSFPARVPWFARSSSDAREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
HHHHHHHHCCCCCCCEEECCCCCCHHHEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
QGASGNRQGALYPLLSQHDPALARFFPAAFTFARRMYDALPVMFDHQWCGVTQLGWDEKS
CCCCCCCCCCEECHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHH
THKIAQMLALNLPPDIACAVTAEQVAGLTGVDTGCGGITYPAGGWLCPQQLTAELLALAA
HHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TRGLHVHYGYPVETLSAEGDGWLLNQQRYHQAVVLANGHRITGFAQTAQLPVYPVGGQVS
HCCEEEEECCCHHEECCCCCCEEECHHHHCEEEEEECCCEEEEEHHHCCCCEEECCCCEE
HIPTTPRLAALRQVLCYDGYLTPQNPQNQQHCIGASYHRGNTDTTFSEEDQQHNRQRLID
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
CFPGAEWPQDVDISANDARCGVRCATRDHLPMVGNVPDYAATLTQYASLHEQPDIADSAP
HCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCH
VCRNLFMLGALGSRGLCTAPLSAELLAAQMSAEPLPLDSDTLAALNPNRLWVRKLLKGKA
HHHHHHHHHCCCCCCCEECCCHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHCCCC
VK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA