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The map label for this gene is prsA [H]

Identifier: 152970785

GI number: 152970785

Start: 2454275

End: 2455213

Strand: Direct

Name: prsA [H]

Synonym: KPN_02238

Alternate gene names: 152970785

Gene position: 2454275-2455213 (Clockwise)

Preceding gene: 152970784

Following gene: 152970786

Centisome position: 46.18

GC content: 53.99

Gene sequence:

>939_bases
ATGAAGCTTTTTGCTGGTAACGCCACCCCGGAACTAGCACAACGTATTGCCAACCGCCTGTACACTTCTCTTGGCGACGC
CGCTGTAGGTCGTTTTAGCGACGGCGAAGTCAGCGTACAAATCAATGAAAACGTACGCGGTGGTGATATTTTCATCATCC
AGTCCACTTGTGCTCCCACCAATGACAACCTGATGGAACTGGTTGTTATGGTTGATGCTCTGCGTCGTGCTTCGGCAGGT
CGTATCACCGCCGTTATCCCTTACTTCGGTTATGCTCGTCAGGACCGTCGTGTTCGTTCTGCTCGTGTGCCAATCACCGC
GAAAGTGGTGGCGGATTTTCTGTCCAGCGTCGGCGTTGACCGCGTGCTGACCGTTGACCTGCACGCAGAACAGATCCAGG
GCTTCTTCGATGTGCCAGTCGATAACGTGTTCGGCAGCCCAATCCTGCTGGAAGATATGCTGCAGCTGAACCTGGACAAC
CCGATTGTGGTTTCTCCGGACATCGGCGGCGTCGTGCGTGCCCGCGCTATCGCTAAACTGCTGAATGATACCGATATGGC
TATCATCGACAAGCGCCGTCCGCGCGCCAACGTCTCCCAGGTGATGCACATCATCGGCGACGTTGCCGGTCGTGACTGCG
TGATGGTCGACGATATGATCGACACCGGCGGCACCCTGTGTAAAGCGGCAGAAGCGCTGAAAGAGCGTGGCGCGAAACGC
GTATTCGCTTACGCGACGCACCCAATCTTCTCCGGCAACGCTATCCAGAACATCAAAAACTCTGTCATTGATGAATTTGT
CGTCTGCGATACCATTCCGCTGGCACCAGAAATTAAAGCGCTGGATAAAGTGCGTACTCTGACGCTCTCCGGTATGCTGG
CTGAAGCTATTCGCCGTATCAGCAATGAAGAATCTATCTCTGCTATGTTCGAACATTAA

Upstream 100 bases:

>100_bases
TCTTTAAATACACCGACTGGATCGAATGGCGCCATCCCGCACATTATTGGCCCATTCATCCACCACTGGACGCATGCCTG
AGGTTCTTCTCGTGCCTGAT

Downstream 100 bases:

>100_bases
TCGAGCCCAGCACAAAAACCCGCTGCGGCGGGTTTTTTTGTCTCCGCACCTTATTTGTATGATCAATGCCTCCTTCACCT
GCCATTTAGTTGACAGATGA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAG
RITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNLDN
PIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVMVDDMIDTGGTLCKAAEALKERGAKR
VFAYATHPIFSGNAIQNIKNSVIDEFVVCDTIPLAPEIKALDKVRTLTLSGMLAEAIRRISNEESISAMFEH

Sequences:

>Translated_312_residues
MKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAG
RITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNLDN
PIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVMVDDMIDTGGTLCKAAEALKERGAKR
VFAYATHPIFSGNAIQNIKNSVIDEFVVCDTIPLAPEIKALDKVRTLTLSGMLAEAIRRISNEESISAMFEH
>Mature_312_residues
MKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAG
RITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNLDN
PIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVMVDDMIDTGGTLCKAAEALKERGAKR
VFAYATHPIFSGNAIQNIKNSVIDEFVVCDTIPLAPEIKALDKVRTLTLSGMLAEAIRRISNEESISAMFEH

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=315, Percent_Identity=48.2539682539683, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI4506129, Length=312, Percent_Identity=48.3974358974359, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI84875539, Length=314, Percent_Identity=48.0891719745223, Blast_Score=295, Evalue=3e-80,
Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=47.4522292993631, Blast_Score=289, Evalue=2e-78,
Organism=Homo sapiens, GI4506133, Length=343, Percent_Identity=36.1516034985423, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI194018537, Length=343, Percent_Identity=36.734693877551, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI310128524, Length=145, Percent_Identity=32.4137931034483, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI310115209, Length=145, Percent_Identity=32.4137931034483, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI310118259, Length=145, Percent_Identity=32.4137931034483, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI310119946, Length=145, Percent_Identity=32.4137931034483, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1787458, Length=312, Percent_Identity=96.474358974359, Blast_Score=608, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=45.859872611465, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=45.859872611465, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=45.859872611465, Blast_Score=286, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=45.8333333333333, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17570245, Length=339, Percent_Identity=34.2182890855457, Blast_Score=194, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6320946, Length=315, Percent_Identity=45.7142857142857, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6321776, Length=314, Percent_Identity=47.7707006369427, Blast_Score=269, Evalue=5e-73,
Organism=Saccharomyces cerevisiae, GI6319403, Length=316, Percent_Identity=45.253164556962, Blast_Score=267, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6322667, Length=202, Percent_Identity=40.5940594059406, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324511, Length=115, Percent_Identity=41.7391304347826, Blast_Score=102, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21355239, Length=314, Percent_Identity=46.8152866242038, Blast_Score=282, Evalue=2e-76,
Organism=Drosophila melanogaster, GI45551540, Length=336, Percent_Identity=43.75, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24651458, Length=352, Percent_Identity=34.9431818181818, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI24651456, Length=352, Percent_Identity=34.9431818181818, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI281362873, Length=352, Percent_Identity=34.9431818181818, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24651454, Length=352, Percent_Identity=34.9431818181818, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24651462, Length=371, Percent_Identity=33.1536388140162, Blast_Score=191, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24651464, Length=371, Percent_Identity=33.1536388140162, Blast_Score=191, Evalue=4e-49,
Organism=Drosophila melanogaster, GI45552010, Length=371, Percent_Identity=33.1536388140162, Blast_Score=191, Evalue=5e-49,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 33952; Mature: 33952

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPT
CEEECCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCC
NDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVD
CCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
RVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNLDNPIVVSPDIGGVVRARAIAKL
EEEEEEEEHHHHCCEECCCHHHHCCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
LNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVMVDDMIDTGGTLCKAAEALKERGAKR
HCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHHHHHHHCCCCE
VFAYATHPIFSGNAIQNIKNSVIDEFVVCDTIPLAPEIKALDKVRTLTLSGMLAEAIRRI
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SNEESISAMFEH
CCHHHHHHHHCC
>Mature Secondary Structure
MKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPT
CEEECCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCC
NDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVD
CCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
RVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNLDNPIVVSPDIGGVVRARAIAKL
EEEEEEEEHHHHCCEECCCHHHHCCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
LNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVMVDDMIDTGGTLCKAAEALKERGAKR
HCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHHHHHHHCCCCE
VFAYATHPIFSGNAIQNIKNSVIDEFVVCDTIPLAPEIKALDKVRTLTLSGMLAEAIRRI
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SNEESISAMFEH
CCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]