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The map label for this gene is rstB [H]

Identifier: 152970616

GI number: 152970616

Start: 2265206

End: 2266267

Strand: Direct

Name: rstB [H]

Synonym: KPN_02067

Alternate gene names: 152970616

Gene position: 2265206-2266267 (Clockwise)

Preceding gene: 152970615

Following gene: 152970617

Centisome position: 42.62

GC content: 42.94

Gene sequence:

>1062_bases
ATGAACAAAGAAACGATTCTGAGCCGTCAGATTTTGACATATATGTTGTTGTTAACGTTTGTTATTATTGCCATTGCAAT
ACTCGGTTCCTACCTATTTTATAGTTTCCTGATTTATTACCTGCCGGGTGGAATGAACGAAGGTAGTGAAGATGCTATGA
CATTCCTTGACTGGATGTGGATTCTGATGGCGTCCATTACCTGTCTGGTCGTTGCACTTTTCTTTACCGTAAAGCTGTCT
GCAAGAATATTCAACCCACTGAATGACGTTGCATACAGCCTGAAACAAATCTCTCAGGGAAACCTTAGTGCCAGAGCATT
TAACAGCAGTTCTAAGTTGGGAGAGATGAATAGGCTCGTCAATGACTTTAATGAGATGGCTGAAAAGTTACAGACGCTGG
ATGCGCAGCGAAATTTATGGAATGCTGCAATTGCTCATGAACTCCGGACGCCGGTGACTATCTTACGTGGGAGGCTTCAG
GGACTGGTTGACGGCGTTTTCGAACCAGAACCGTCGTTATTCAGAAACCTTCTTAAACAAACTGAAGGTCTGACCAACTT
AATTGAAGATCTCCGCGTCGTGAGTTCTTCAGGAGGCGCAGGGTACTCCTTGATGCTGAGTGAAGTGGATCTCAAGGCCA
CGATTTCAAATGCGCTTGATACCTTTTGGCCGGACTTTGATAAAAAGCATTTCAAAATTGTCACTGAGATTAACCAGCAG
TATTGCGTTTGCGATCCCTTACGCATTATTCAGTGCCTCACCGTTCTTTTTGACAATGCCTTAAAATATTCCACATCACA
AACGCTGTTAATAAAGAATGGTATTACAGGAAACGATAATTTTATTATCGTTCAGGACCAAGGACCTGGAATTCCTGAAG
AACTCCAGAAGTCCTTATTCCAGCCTTTTCAGCGAGGAGAATACGCCAAAAATATTAATCCAGAGGGCTGTGGTTTAGGG
TTGTCGGTAGTTAAAGCCATTATGTGCGCCCACGGTGGAGACGTTAGCTATAGTTTAACACCAGAAAATGGCTCACTTTT
CAAACTCTCATGGCCTGTTTAA

Upstream 100 bases:

>100_bases
ACAGTCATATGAGTAAACTTCGGAAAAAGCTTGAACATGCCGGATTGAAGGGCATTCCGGAAAGCATCCGAGGATTAGGC
TACCGGTTGGGTGACAAAAA

Downstream 100 bases:

>100_bases
GGGCATGAATACACAGGCTCGATCGAACCTGTTTTAGCAAGAGACAGATCCCACATACTGCCGAGTTTTGCGTCCCAAAT
TGATGGAGTGATTTTAAGTT

Product: sensor histidine protein kinase (RstA regulator)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MNKETILSRQILTYMLLLTFVIIAIAILGSYLFYSFLIYYLPGGMNEGSEDAMTFLDWMWILMASITCLVVALFFTVKLS
ARIFNPLNDVAYSLKQISQGNLSARAFNSSSKLGEMNRLVNDFNEMAEKLQTLDAQRNLWNAAIAHELRTPVTILRGRLQ
GLVDGVFEPEPSLFRNLLKQTEGLTNLIEDLRVVSSSGGAGYSLMLSEVDLKATISNALDTFWPDFDKKHFKIVTEINQQ
YCVCDPLRIIQCLTVLFDNALKYSTSQTLLIKNGITGNDNFIIVQDQGPGIPEELQKSLFQPFQRGEYAKNINPEGCGLG
LSVVKAIMCAHGGDVSYSLTPENGSLFKLSWPV

Sequences:

>Translated_353_residues
MNKETILSRQILTYMLLLTFVIIAIAILGSYLFYSFLIYYLPGGMNEGSEDAMTFLDWMWILMASITCLVVALFFTVKLS
ARIFNPLNDVAYSLKQISQGNLSARAFNSSSKLGEMNRLVNDFNEMAEKLQTLDAQRNLWNAAIAHELRTPVTILRGRLQ
GLVDGVFEPEPSLFRNLLKQTEGLTNLIEDLRVVSSSGGAGYSLMLSEVDLKATISNALDTFWPDFDKKHFKIVTEINQQ
YCVCDPLRIIQCLTVLFDNALKYSTSQTLLIKNGITGNDNFIIVQDQGPGIPEELQKSLFQPFQRGEYAKNINPEGCGLG
LSVVKAIMCAHGGDVSYSLTPENGSLFKLSWPV
>Mature_353_residues
MNKETILSRQILTYMLLLTFVIIAIAILGSYLFYSFLIYYLPGGMNEGSEDAMTFLDWMWILMASITCLVVALFFTVKLS
ARIFNPLNDVAYSLKQISQGNLSARAFNSSSKLGEMNRLVNDFNEMAEKLQTLDAQRNLWNAAIAHELRTPVTILRGRLQ
GLVDGVFEPEPSLFRNLLKQTEGLTNLIEDLRVVSSSGGAGYSLMLSEVDLKATISNALDTFWPDFDKKHFKIVTEINQQ
YCVCDPLRIIQCLTVLFDNALKYSTSQTLLIKNGITGNDNFIIVQDQGPGIPEELQKSLFQPFQRGEYAKNINPEGCGLG
LSVVKAIMCAHGGDVSYSLTPENGSLFKLSWPV

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=306, Percent_Identity=31.3725490196078, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788393, Length=273, Percent_Identity=27.8388278388278, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1786783, Length=308, Percent_Identity=25, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789808, Length=280, Percent_Identity=27.8571428571429, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786912, Length=287, Percent_Identity=27.1777003484321, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081816, Length=259, Percent_Identity=25.4826254826255, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1789149, Length=330, Percent_Identity=24.5454545454545, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082128, Length=298, Percent_Identity=22.4832214765101, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788279, Length=212, Percent_Identity=26.4150943396226, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1789403, Length=298, Percent_Identity=24.1610738255034, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=24.1803278688525, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788713, Length=222, Percent_Identity=26.5765765765766, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790551, Length=281, Percent_Identity=28.4697508896797, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790346, Length=287, Percent_Identity=24.7386759581882, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788549, Length=232, Percent_Identity=25.8620689655172, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39400; Mature: 39400

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCC
>Mature Secondary Structure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HHHHCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]