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The map label for this gene is rnfE [H]

Identifier: 152970521

GI number: 152970521

Start: 2166118

End: 2166816

Strand: Direct

Name: rnfE [H]

Synonym: KPN_01969

Alternate gene names: 152970521

Gene position: 2166118-2166816 (Clockwise)

Preceding gene: 152970520

Following gene: 152970522

Centisome position: 40.75

GC content: 60.66

Gene sequence:

>699_bases
ATGAGCGAAGTTAAAGACGTCATCGTCCAGGGCCTGTGGAAAAATAACTCCGCCCTGGTTCAGCTGTTAGGTATGTGTCC
TCTGCTGGCGGTCACCTCGACCGCCACCAACGCCCTCGGGCTGGGTTTGGCCACCACCCTGGTGTTAACCCTGACCAACC
TGACCATCTCCAGCCTGCGCCGCTGGACGCCGGCGGAGATCCGGATCCCCATCTACGTAATGATTATCGCCTCGGTGGTG
AGCGTCGTGCAGATGCTGATCAACGCCTATGCCTTCGGCCTCTATCAGTCGCTGGGGATTTTCATCCCGCTTATCGTGAC
CAACTGCATCGTGGTCGGCCGCGCCGAAGCCTTTGCCGCCAAAAAAGGGCCGGCGCTGTCGGCGCTGGACGGTTTCTCGA
TCGGCATGGGCGCTACCTGCGCGATGTTTGTGCTGGGCTCCCTGCGCGAGATCCTTGGCAACGGCACCCTGTTTGACGGC
GCCGACAGTCTGCTTGGCAGCTGGGCGAAGGTTCTGCGCATCGAGGTTTTCCACGCCGACACCCCTTTCCTGCTGGCTAT
GCTGCCGCCAGGCGCCTTTATTGGCCTCGGCATGATGCTGGCGATTAAATACCTTATCGATGAACGTAGCAAACAGCGGA
AGGCGCAAGCGGCCCGGGCCGTCTCTGTCGCCCCGTCCGACGTGACAGGGAAAGCATAA

Upstream 100 bases:

>100_bases
TCACCCCCCGGGCGGTGGTCAATGCCGTTAAGCGCGCAGGCTTATATGCACAAACGCTGCCTGCGCAACTTCCTGAATTC
ACCGCCTGTGGAGAATAACC

Downstream 100 bases:

>100_bases
TATGAACAAAGCCAAACGCCTGGCGATCCTTACCAGGCTGCGGGAAAACGATCCCCACCCCACTACCGAACTGCATTTCA
GTTCGCCGTTCGAGCTGCTG

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: Nitrogen fixation protein rnfE [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MSEVKDVIVQGLWKNNSALVQLLGMCPLLAVTSTATNALGLGLATTLVLTLTNLTISSLRRWTPAEIRIPIYVMIIASVV
SVVQMLINAYAFGLYQSLGIFIPLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREILGNGTLFDG
ADSLLGSWAKVLRIEVFHADTPFLLAMLPPGAFIGLGMMLAIKYLIDERSKQRKAQAARAVSVAPSDVTGKA

Sequences:

>Translated_232_residues
MSEVKDVIVQGLWKNNSALVQLLGMCPLLAVTSTATNALGLGLATTLVLTLTNLTISSLRRWTPAEIRIPIYVMIIASVV
SVVQMLINAYAFGLYQSLGIFIPLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREILGNGTLFDG
ADSLLGSWAKVLRIEVFHADTPFLLAMLPPGAFIGLGMMLAIKYLIDERSKQRKAQAARAVSVAPSDVTGKA
>Mature_231_residues
SEVKDVIVQGLWKNNSALVQLLGMCPLLAVTSTATNALGLGLATTLVLTLTNLTISSLRRWTPAEIRIPIYVMIIASVVS
VVQMLINAYAFGLYQSLGIFIPLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREILGNGTLFDGA
DSLLGSWAKVLRIEVFHADTPFLLAMLPPGAFIGLGMMLAIKYLIDERSKQRKAQAARAVSVAPSDVTGKA

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787919, Length=212, Percent_Identity=92.9245283018868, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1787914, Length=156, Percent_Identity=35.2564102564103, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR010968 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 24528; Mature: 24396

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVKDVIVQGLWKNNSALVQLLGMCPLLAVTSTATNALGLGLATTLVLTLTNLTISSLR
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWTPAEIRIPIYVMIIASVVSVVQMLINAYAFGLYQSLGIFIPLIVTNCIVVGRAEAFAA
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KKGPALSALDGFSIGMGATCAMFVLGSLREILGNGTLFDGADSLLGSWAKVLRIEVFHAD
CCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHEEECC
TPFLLAMLPPGAFIGLGMMLAIKYLIDERSKQRKAQAARAVSVAPSDVTGKA
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SEVKDVIVQGLWKNNSALVQLLGMCPLLAVTSTATNALGLGLATTLVLTLTNLTISSLR
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWTPAEIRIPIYVMIIASVVSVVQMLINAYAFGLYQSLGIFIPLIVTNCIVVGRAEAFAA
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KKGPALSALDGFSIGMGATCAMFVLGSLREILGNGTLFDGADSLLGSWAKVLRIEVFHAD
CCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHEEECC
TPFLLAMLPPGAFIGLGMMLAIKYLIDERSKQRKAQAARAVSVAPSDVTGKA
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA