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The map label for this gene is rnfA [H]

Identifier: 152970516

GI number: 152970516

Start: 2161129

End: 2161713

Strand: Direct

Name: rnfA [H]

Synonym: KPN_01964

Alternate gene names: 152970516

Gene position: 2161129-2161713 (Clockwise)

Preceding gene: 152970515

Following gene: 152970517

Centisome position: 40.66

GC content: 53.5

Gene sequence:

>585_bases
ATGATGGCTGATTATTTACTGCTCTTTATTGGTACAGTCCTGGTCAATAACTTCGTTCTGGTGAAGTTTCTTGGTCTGTG
CCCGTTTATGGGGGTTTCCAAAAAGCTGGAAACCGCAATGGGGATGGGGCTCGCTACCACCTTCGTGATGACCCTGGCCT
CTATTTGCGCCTGGCTGATTGATACCTGGATCCTTATCCCGCTAAATTTGGTTTATTTGCGTACCCTGGCCTTTATTCTG
GTTATCGCCGTGGTGGTTCAGTTTACTGAGATGGTGGTGCGTAAAACCAGCCCGGCGCTCTATCGCCTGCTGGGGATCTT
TCTACCGCTGATCACCACCAACTGTGCCGTACTCGGGGTAGCGCTGCTGAATATTAATCTGGGCCACAATTTCCTGCAGT
CGGCGCTGTACGGCTTTGCCGCCGCCGTCGGCTTCTCGCTGGTAATGGTGCTGTTCGCGGCGATCCGTGAACGCCTGGTG
GTGGCCGATGTCCCCGCGCCGTTTCGCGGTAATGCCATCGCGCTGATTACCGCCGGTTTAATGTCTCTGGCCTTCATGGG
CTTTAGTGGTTTGGTGAAGTTGTAA

Upstream 100 bases:

>100_bases
AAAACGGACTTTTGTTAATCCGTAATACCCCCACACTTAACCTGTTGTCGCTGGTATCGTTTTATTCGATAATAAGCGGC
TTTTTTTCGTTTCAGGTCGC

Downstream 100 bases:

>100_bases
TGAGCGCAGTCTGGATAGCCGTGATCGCCATCAGCCTGTTAGGGCTGATTTTCGGCTTAATACTGGGATACGCCTCGCGT
CGTTTCGCGGTGCAGGACGA

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MMADYLLLFIGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLATTFVMTLASICAWLIDTWILIPLNLVYLRTLAFIL
VIAVVVQFTEMVVRKTSPALYRLLGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFAAAVGFSLVMVLFAAIRERLV
VADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL

Sequences:

>Translated_194_residues
MMADYLLLFIGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLATTFVMTLASICAWLIDTWILIPLNLVYLRTLAFIL
VIAVVVQFTEMVVRKTSPALYRLLGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFAAAVGFSLVMVLFAAIRERLV
VADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL
>Mature_194_residues
MMADYLLLFIGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLATTFVMTLASICAWLIDTWILIPLNLVYLRTLAFIL
VIAVVVQFTEMVVRKTSPALYRLLGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFAAAVGFSLVMVLFAAIRERLV
VADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL

Specific function: May be part of a membrane complex involved in electron transport [H]

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787914, Length=193, Percent_Identity=96.8911917098446, Blast_Score=369, Evalue=1e-104,
Organism=Escherichia coli, GI1787919, Length=142, Percent_Identity=36.6197183098592, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR011293 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 20983; Mature: 20983

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMADYLLLFIGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLATTFVMTLASICAWLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTWILIPLNLVYLRTLAFILVIAVVVQFTEMVVRKTSPALYRLLGIFLPLITTNCAVLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ALLNINLGHNFLQSALYGFAAAVGFSLVMVLFAAIRERLVVADVPAPFRGNAIALITAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
MSLAFMGFSGLVKL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MMADYLLLFIGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLATTFVMTLASICAWLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTWILIPLNLVYLRTLAFILVIAVVVQFTEMVVRKTSPALYRLLGIFLPLITTNCAVLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ALLNINLGHNFLQSALYGFAAAVGFSLVMVLFAAIRERLVVADVPAPFRGNAIALITAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
MSLAFMGFSGLVKL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA