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The map label for this gene is narY [H]

Identifier: 152970433

GI number: 152970433

Start: 2074657

End: 2076201

Strand: Direct

Name: narY [H]

Synonym: KPN_01881

Alternate gene names: 152970433

Gene position: 2074657-2076201 (Clockwise)

Preceding gene: 152970432

Following gene: 152970434

Centisome position: 39.03

GC content: 59.22

Gene sequence:

>1545_bases
ATGAAAATCCGTTCTCAGGTTGGAATGGTGCTGAATCTGGACAAATGCATCGGCTGTCACACCTGCTCGGTCACCTGTAA
AAACGTCTGGAGCAGCCGCGAAGGCATGGAGTACGCGTGGTTCAATAACGTGGAGACCAAACCGGGCATTGGCTACCCCA
AAAACTGGGAGGACCAGGATCAGTGGCAAGGCGGCTGGATCCGCGGCATTAGCGGTAAGCTGACGCCCCGCCTCGGCAAT
CGGGTCAGCGTGTTATCGAAGATTTTCGCCAACCCGGTGCTGCCGGCGATCGACGATTACTACGAACCCTTTACCTACGA
CTACCAGCATCTGCACAACGCGCCGGAGGGCAAATATCTGCCGACCGCCCGCCCGCGCTCGCTGATCAGCGGCGAGCGTA
TGGATAAGATTAACTGGGGCCCGAACTGGGAGGAGCTGCTCGGCGGCGAATTCGAGAAACGCGCCCGCGACCGTAACTTC
GAAGCCATGCAAAAAGAGATGTACGGCCAGTTCGAAAATACCTTCATGATGTATCTCCCGCGCCTGTGCGAGCACTGCCT
CAACCCGAGCTGCGTGGCCACCTGCCCGAGCGGCGCCATCTACAAGCGTGAGGAAGATGGCATCGTACTGATCGATCAGG
ATAAATGCCGCGGCTGGCGGATGTGCATCAGCGGCTGCCCGTACAAAAAAATCTACTTTAACTGGAAGAGCGGTAAGTCG
GAAAAATGCATCTTCTGCTATCCGCGCATTGAGTCCGGGCAGCCAACGGTCTGCTCAGAAACCTGCGTCGGGCGCATCCG
CTATCTTGGCGTACTGCTGTATGACGCCGACCGCATTGAAGAGGCGGCCAGCACCGAACATGAAACCGACCTCTATGAGC
GCCAGTGCGAGGTGTTCCTTAATCCAAACGATCCGGCGGTCATCAAGGAAGCGCTAAAGCAAGGCATCCCGCACAACGTC
ATTGAGGCGGCGCAGAAATCGCCGGTCTATAAGCTGGCGATGGACTGGAAGCTGGCTCTGCCGCTGCATCCGGAGTATCG
CACCCTGCCGATGGTCTGGTACGTGCCGCCCCTGTCGCCGATCCAGTCGGTTGCCGACGCCGGCGGCTTACCGAGCAACG
GCAACATCCTGCCGGCGGTTGAGAGTCTGCGCATCCCGGTGCAATACCTGGCGAATCTGCTGAGCGCCGGCGATACCGGA
CCGGTCTTGCGCGCGCTGAAACGGATGATGGCGATGCGCCACTACAAGCGCTCGCAAACCGTCGAGGGCGTCACCGATAC
CCGCGCCATTGAAGAGGTGGGCCTCAGCGTTGAGCAGGTCGAAGAGATGTATCGCTATCTGGCGATCGCCAACTACGAAG
ACCGCTTCGTGATCCCCACCAGCCATCGCGAACTGGCGGAGGATGCCTTTCCGGAACGTAACGGCTGCGGCTTTACCTTC
GGCGATGGCTGCCACGGGTCAGATACGAAATTCAACCTGTTTAACAGCCGGCGGATCGACGCCATCGACGTCAGCGGCGT
GCGTAAGCATGGGGAGGGCGAGTGA

Upstream 100 bases:

>100_bases
CCGTCGGATCGAACCGCGACGAGTTCATCATGATCCGCAAAATGAAGAATATTGCCTGGCTGGATGACGAAGGTCGCGAT
CAGGTACAGGAGGCGAAAAA

Downstream 100 bases:

>100_bases
TGCGGATCCTGAAAGTGATCGGTCTGCTGCTGGAGTACCCCGACGAGTTACTGTGGGAGAACCGCGACGACGCCCTGGCG
CTGATCAGGGCCGATGTCCC

Product: cryptic nitrate reductase 2 beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWSSREGMEYAWFNNVETKPGIGYPKNWEDQDQWQGGWIRGISGKLTPRLGN
RVSVLSKIFANPVLPAIDDYYEPFTYDYQHLHNAPEGKYLPTARPRSLISGERMDKINWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCEVFLNPNDPAVIKEALKQGIPHNV
IEAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSVADAGGLPSNGNILPAVESLRIPVQYLANLLSAGDTG
PVLRALKRMMAMRHYKRSQTVEGVTDTRAIEEVGLSVEQVEEMYRYLAIANYEDRFVIPTSHRELAEDAFPERNGCGFTF
GDGCHGSDTKFNLFNSRRIDAIDVSGVRKHGEGE

Sequences:

>Translated_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWSSREGMEYAWFNNVETKPGIGYPKNWEDQDQWQGGWIRGISGKLTPRLGN
RVSVLSKIFANPVLPAIDDYYEPFTYDYQHLHNAPEGKYLPTARPRSLISGERMDKINWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCEVFLNPNDPAVIKEALKQGIPHNV
IEAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSVADAGGLPSNGNILPAVESLRIPVQYLANLLSAGDTG
PVLRALKRMMAMRHYKRSQTVEGVTDTRAIEEVGLSVEQVEEMYRYLAIANYEDRFVIPTSHRELAEDAFPERNGCGFTF
GDGCHGSDTKFNLFNSRRIDAIDVSGVRKHGEGE
>Mature_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWSSREGMEYAWFNNVETKPGIGYPKNWEDQDQWQGGWIRGISGKLTPRLGN
RVSVLSKIFANPVLPAIDDYYEPFTYDYQHLHNAPEGKYLPTARPRSLISGERMDKINWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCEVFLNPNDPAVIKEALKQGIPHNV
IEAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSVADAGGLPSNGNILPAVESLRIPVQYLANLLSAGDTG
PVLRALKRMMAMRHYKRSQTVEGVTDTRAIEEVGLSVEQVEEMYRYLAIANYEDRFVIPTSHRELAEDAFPERNGCGFTF
GDGCHGSDTKFNLFNSRRIDAIDVSGVRKHGEGE

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=514, Percent_Identity=88.3268482490272, Blast_Score=979, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=506, Percent_Identity=82.2134387351779, Blast_Score=889, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=99, Percent_Identity=42.4242424242424, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=41.5841584158416, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367345, Length=111, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=37.3737373737374, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=36.046511627907, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58439; Mature: 58439

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWSSREGMEYAWFNNVETKPGIGYPKNWEDQD
CCCHHHCCCEEEHHHHHCCCCHHEEHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCH
QWQGGWIRGISGKLTPRLGNRVSVLSKIFANPVLPAIDDYYEPFTYDYQHLHNAPEGKYL
HHCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHCCCCCCCCC
PTARPRSLISGERMDKINWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLP
CCCCCHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCEVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCCHHHHHHHHCCEEEE
NPNDPAVIKEALKQGIPHNVIEAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCCH
IQSVADAGGLPSNGNILPAVESLRIPVQYLANLLSAGDTGPVLRALKRMMAMRHYKRSQT
HHHHHHCCCCCCCCCEECCHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
VEGVTDTRAIEEVGLSVEQVEEMYRYLAIANYEDRFVIPTSHRELAEDAFPERNGCGFTF
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCCCCC
GDGCHGSDTKFNLFNSRRIDAIDVSGVRKHGEGE
CCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWSSREGMEYAWFNNVETKPGIGYPKNWEDQD
CCCHHHCCCEEEHHHHHCCCCHHEEHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCH
QWQGGWIRGISGKLTPRLGNRVSVLSKIFANPVLPAIDDYYEPFTYDYQHLHNAPEGKYL
HHCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHCCCCCCCCC
PTARPRSLISGERMDKINWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLP
CCCCCHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCEVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCCHHHHHHHHCCEEEE
NPNDPAVIKEALKQGIPHNVIEAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCCH
IQSVADAGGLPSNGNILPAVESLRIPVQYLANLLSAGDTGPVLRALKRMMAMRHYKRSQT
HHHHHHCCCCCCCCCEECCHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
VEGVTDTRAIEEVGLSVEQVEEMYRYLAIANYEDRFVIPTSHRELAEDAFPERNGCGFTF
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCCCCC
GDGCHGSDTKFNLFNSRRIDAIDVSGVRKHGEGE
CCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]