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The map label for this gene is narU [H]

Identifier: 152970431

GI number: 152970431

Start: 2069699

End: 2070805

Strand: Direct

Name: narU [H]

Synonym: KPN_01879

Alternate gene names: 152970431

Gene position: 2069699-2070805 (Clockwise)

Preceding gene: 152970430

Following gene: 152970432

Centisome position: 38.94

GC content: 58.36

Gene sequence:

>1107_bases
ATGGTACCCCTTTTTGGCGGGCGCTACTGGACCGTATTAAGTACCGTTATCCTGATCGTGCCTTGTATTTGGTTAGGGGT
GGCAATCCAGAATATTACTACTCCGTTCTGGGTATTTATTATTATTGCGCTGCTGTGCGGTTTTGCCGGAGCGAATTTTG
CTTCCAGCATGGGAAATATCAGTTTCTTTTTCCCCAAAGCGAAACAGGGCAGCGCGCTGGGAGTCAACGGCGGCCTTGGC
AATCTGGGCGTAAGCGTGATGCAGATGGTCGCCCCGGCGGTCATCTTCCTGCCGCTCTTCACCTTTCTCGGCGTCCATGG
CGTCACCCAGCCCGATGGTTCGACAATCACCCTGAGTAATGCGGCGCTCGTCTGGGTACCGCTGCTGCTGCTCGCCACCG
TCGCGGCATGGTTCGGCATGAACGATATCGCCGGTTCAAAGGCCTCGATTCGCGATCAGCTTCCCGTGCTCAAGCGACCG
CATATGTGGCTGTTAAGCCTGCTCTATCTGGCGACGTTTGGCTCGTTCATCGGCTTTTCCGCCGGCTTTGCCATGCTGGC
GAAAACCCAGTTTCCCGCGGTCGATATCCTCAAGCTCGCCTTCTTTGGACCGTTCATCGGCGCGCTGGCGCGCTCGTTCG
GCGGGATCATCTCCGACCGTCTCGGCGGCGTGCGGGTGACGCTGGTTAACTTCGTCCTGATGGCGCTGTTTACCGGCCTG
CTGTTTCTGACCCTGCCAGGCAGCGGCTCCGGCAGCTTTCTCGCTTTCTACGTGGTGTTTATGGGGCTCTTTTTAACCGC
CGGTCTGGGAAGCGGCTCCACCTTCCAGATGATCGCCGTCATCTTCCGCCAGCTCACCATCGACAGCGTAAAACAGCGCG
GCGGCAGCGATGAAGAAGCGCAGCACGAGGCGGTGACCGATACCGCTGCCGCGCTGGGCTTTATCTCGGCGATCGGCGCC
ATCGGCGGCTTCTTTATTCCGAAAGCCTTCGGCACCTCGCTGGCGATGACCGGCTCACCGGTTGGCGCGATGAAAGTCTT
TTTTGTCTTCTATGTGGTTTGCGTGCTGGTGACCTGGCTGGTCTACGGCCGCCGTAAATCCGCTTAA

Upstream 100 bases:

>100_bases
TCTCAATAAAGTCGGTTTTCATTTCACCACCGACCAGCTTTTTTTATTAACCGCCCTGCCTTCACTTTCCGGCGCGATAT
TACGGGTCCCCTACTCCTTT

Downstream 100 bases:

>100_bases
CCATAACGATTTATCTGTCGGATAGCGGCTTACGCCTTATCCGGCCTTACGAGTTCGTTCGCTCATCCGGCCGGGTGGCA
CGATGCCGGACATTATCGGA

Product: MFS family nitrate extrusion protein

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator 2 [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MVPLFGGRYWTVLSTVILIVPCIWLGVAIQNITTPFWVFIIIALLCGFAGANFASSMGNISFFFPKAKQGSALGVNGGLG
NLGVSVMQMVAPAVIFLPLFTFLGVHGVTQPDGSTITLSNAALVWVPLLLLATVAAWFGMNDIAGSKASIRDQLPVLKRP
HMWLLSLLYLATFGSFIGFSAGFAMLAKTQFPAVDILKLAFFGPFIGALARSFGGIISDRLGGVRVTLVNFVLMALFTGL
LFLTLPGSGSGSFLAFYVVFMGLFLTAGLGSGSTFQMIAVIFRQLTIDSVKQRGGSDEEAQHEAVTDTAAALGFISAIGA
IGGFFIPKAFGTSLAMTGSPVGAMKVFFVFYVVCVLVTWLVYGRRKSA

Sequences:

>Translated_368_residues
MVPLFGGRYWTVLSTVILIVPCIWLGVAIQNITTPFWVFIIIALLCGFAGANFASSMGNISFFFPKAKQGSALGVNGGLG
NLGVSVMQMVAPAVIFLPLFTFLGVHGVTQPDGSTITLSNAALVWVPLLLLATVAAWFGMNDIAGSKASIRDQLPVLKRP
HMWLLSLLYLATFGSFIGFSAGFAMLAKTQFPAVDILKLAFFGPFIGALARSFGGIISDRLGGVRVTLVNFVLMALFTGL
LFLTLPGSGSGSFLAFYVVFMGLFLTAGLGSGSTFQMIAVIFRQLTIDSVKQRGGSDEEAQHEAVTDTAAALGFISAIGA
IGGFFIPKAFGTSLAMTGSPVGAMKVFFVFYVVCVLVTWLVYGRRKSA
>Mature_368_residues
MVPLFGGRYWTVLSTVILIVPCIWLGVAIQNITTPFWVFIIIALLCGFAGANFASSMGNISFFFPKAKQGSALGVNGGLG
NLGVSVMQMVAPAVIFLPLFTFLGVHGVTQPDGSTITLSNAALVWVPLLLLATVAAWFGMNDIAGSKASIRDQLPVLKRP
HMWLLSLLYLATFGSFIGFSAGFAMLAKTQFPAVDILKLAFFGPFIGALARSFGGIISDRLGGVRVTLVNFVLMALFTGL
LFLTLPGSGSGSFLAFYVVFMGLFLTAGLGSGSTFQMIAVIFRQLTIDSVKQRGGSDEEAQHEAVTDTAAALGFISAIGA
IGGFFIPKAFGTSLAMTGSPVGAMKVFFVFYVVCVLVTWLVYGRRKSA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=366, Percent_Identity=80.0546448087432, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1787475, Length=368, Percent_Identity=73.9130434782609, Blast_Score=524, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 38956; Mature: 38956

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPLFGGRYWTVLSTVILIVPCIWLGVAIQNITTPFWVFIIIALLCGFAGANFASSMGNI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHCCCE
SFFFPKAKQGSALGVNGGLGNLGVSVMQMVAPAVIFLPLFTFLGVHGVTQPDGSTITLSN
EEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
AALVWVPLLLLATVAAWFGMNDIAGSKASIRDQLPVLKRPHMWLLSLLYLATFGSFIGFS
HHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHH
AGFAMLAKTQFPAVDILKLAFFGPFIGALARSFGGIISDRLGGVRVTLVNFVLMALFTGL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LFLTLPGSGSGSFLAFYVVFMGLFLTAGLGSGSTFQMIAVIFRQLTIDSVKQRGGSDEEA
HHEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
QHEAVTDTAAALGFISAIGAIGGFFIPKAFGTSLAMTGSPVGAMKVFFVFYVVCVLVTWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
VYGRRKSA
HHCCCCCC
>Mature Secondary Structure
MVPLFGGRYWTVLSTVILIVPCIWLGVAIQNITTPFWVFIIIALLCGFAGANFASSMGNI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHCCCE
SFFFPKAKQGSALGVNGGLGNLGVSVMQMVAPAVIFLPLFTFLGVHGVTQPDGSTITLSN
EEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
AALVWVPLLLLATVAAWFGMNDIAGSKASIRDQLPVLKRPHMWLLSLLYLATFGSFIGFS
HHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHH
AGFAMLAKTQFPAVDILKLAFFGPFIGALARSFGGIISDRLGGVRVTLVNFVLMALFTGL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LFLTLPGSGSGSFLAFYVVFMGLFLTAGLGSGSTFQMIAVIFRQLTIDSVKQRGGSDEEA
HHEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
QHEAVTDTAAALGFISAIGAIGGFFIPKAFGTSLAMTGSPVGAMKVFFVFYVVCVLVTWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHH
VYGRRKSA
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2233673; 7747940 [H]