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The map label for this gene is pucI [H]

Identifier: 152970342

GI number: 152970342

Start: 1972135

End: 1973631

Strand: Direct

Name: pucI [H]

Synonym: KPN_01790

Alternate gene names: 152970342

Gene position: 1972135-1973631 (Clockwise)

Preceding gene: 152970338

Following gene: 152970343

Centisome position: 37.1

GC content: 59.85

Gene sequence:

>1497_bases
ATGCCACATTCATCCCACACGCAGCAGACGAAGACTCACAAGGCGGCCGCCGGCTACAGTCCGCGGCTATGTAATGACGA
TCTGGCGCCGACCCGCGACCAGAACTGGAGCTGGTACAATATCTTTTCATTCTGGATGTCGGACGTGCACAGCATGGGCG
GCTACGTAGTGGCGGCGAGCTTCTTTACGCTTGGGCTGGCCAGCTGGCAGGTTCTGCTCTGCCTGCTGGTGGGCATCTGT
ATTGTCCAGCTGTGCGCCAACCTGGTGGCGAAGCCGAGCCAGATGGCCGGGGTGCCTTACGCGGTGATTTGTCGTCAGGC
GTTTGGCGTTTTCGGCGCCAATATCCCGGCGGTGATCCGCGGGCTGATCGCCTTTGCCTGGTACGGCATTCAGACCTATC
TGGCGGCGAATGCCCTGATGCTGGTCCTGCTCAAGTTCTGGCCCTCCCTTGCCTCGCTAACCAGCAGCAGCTTTCTCGGG
CTGTCGCCCCTCGGCTGGCTGTGCTTCGCCACTATGTGGCTGCTGCAGGCGATGGTGTTCTGGCATGGCATGAACGCCAT
CAAACGCTTTATCGATATCGCTGGTCCGGCGGTTTATGTGGTGATGCTGGCGCTGGCCGGCTGGATTGTATACAAGACTG
GTCTGGACGGTATCTCCTTTACCCTCGCCAGCAAATCCCTGAGCGCCGGCGAGCAGACCTGGCAGATGATCACCGCCACG
GCGCTGGTGGTCTCCTACTTCTCCGGGCCGCTGCTTAACTTCGGTGACTTTTCCCGCTACGGCAAAAGCATGGGCGAGAT
CCGCCGCGGTAACCGCTGGGGACTGCCGTTTAACTTTCTGCTATTCTCGGTGGTTACCGTGGTGATTGTCTCCGGCACCC
AGTCGCTGTTTGGCAAAATGATCACCGACCCGATCGAAACCGTCAGCCGCGTGGGTAACGATCTGGCGGTGGCCATCGGC
CTGCTGACCATGATCACCGCCACCATCGGCATTAATATCGTGGCTAACTTCGTCTCGCCGGCCTTCGATTTTTCCAACTG
CGCCCCGCAGAAAATCAGCTTCCGTACCGGCGGGATGATTGCCGCGGTGGGCTCGATACTCCTGACGCCGTGGAACCTGT
TTAACTCGCCGGAGCTGATCCACTATACCCTCGACGTGCTGGGCGCCTTTATTGGCCCGCTGTTTGGCATCCTGATCGCC
GACTTCTACCTGATCAAACGCGGCCGGGTGTCAGTGGACGATCTGTTTGACGATACGCCGCAGGGTAAATATTGGTACCG
CAACGGCTTTAACCCGAAAGCGATTGCCGCCCTGCTGCCGTCGGTGGGCCTGGGGCTGATTATCAGCTTTATTCCGGCCC
TGCATGAAGTGGCGAACTTCAGCTGGTTTATCGGCGTCTTCCTCGGCGCCACCACCTACCGCTGGCTGGCTCGCGACGAG
CGCGAAGTGCAGGCGAAAGCTGCCTTCCGCTCCGGCGCGGTGGCGCAAAAAGAGTAA

Upstream 100 bases:

>100_bases
AAATTTAATTTTTGTATACAAGATTCATCATTCTGGCCTGGATCCTGCAATAGCCTCAGCAATCCATATCAAAGGGTTTT
CATTCAGAGGAGCAGGTTTC

Downstream 100 bases:

>100_bases
CCCTCCCCACCTGCCGCGGCGTCTGGCCGCGGCAGGCTGCAAACTGGCACCAATCTTGCTGTTTATCACTATTCCCAGAG
CGTTAAGAGAAGGCAGGTTC

Product: putative allantoin permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MPHSSHTQQTKTHKAAAGYSPRLCNDDLAPTRDQNWSWYNIFSFWMSDVHSMGGYVVAASFFTLGLASWQVLLCLLVGIC
IVQLCANLVAKPSQMAGVPYAVICRQAFGVFGANIPAVIRGLIAFAWYGIQTYLAANALMLVLLKFWPSLASLTSSSFLG
LSPLGWLCFATMWLLQAMVFWHGMNAIKRFIDIAGPAVYVVMLALAGWIVYKTGLDGISFTLASKSLSAGEQTWQMITAT
ALVVSYFSGPLLNFGDFSRYGKSMGEIRRGNRWGLPFNFLLFSVVTVVIVSGTQSLFGKMITDPIETVSRVGNDLAVAIG
LLTMITATIGINIVANFVSPAFDFSNCAPQKISFRTGGMIAAVGSILLTPWNLFNSPELIHYTLDVLGAFIGPLFGILIA
DFYLIKRGRVSVDDLFDDTPQGKYWYRNGFNPKAIAALLPSVGLGLIISFIPALHEVANFSWFIGVFLGATTYRWLARDE
REVQAKAAFRSGAVAQKE

Sequences:

>Translated_498_residues
MPHSSHTQQTKTHKAAAGYSPRLCNDDLAPTRDQNWSWYNIFSFWMSDVHSMGGYVVAASFFTLGLASWQVLLCLLVGIC
IVQLCANLVAKPSQMAGVPYAVICRQAFGVFGANIPAVIRGLIAFAWYGIQTYLAANALMLVLLKFWPSLASLTSSSFLG
LSPLGWLCFATMWLLQAMVFWHGMNAIKRFIDIAGPAVYVVMLALAGWIVYKTGLDGISFTLASKSLSAGEQTWQMITAT
ALVVSYFSGPLLNFGDFSRYGKSMGEIRRGNRWGLPFNFLLFSVVTVVIVSGTQSLFGKMITDPIETVSRVGNDLAVAIG
LLTMITATIGINIVANFVSPAFDFSNCAPQKISFRTGGMIAAVGSILLTPWNLFNSPELIHYTLDVLGAFIGPLFGILIA
DFYLIKRGRVSVDDLFDDTPQGKYWYRNGFNPKAIAALLPSVGLGLIISFIPALHEVANFSWFIGVFLGATTYRWLARDE
REVQAKAAFRSGAVAQKE
>Mature_497_residues
PHSSHTQQTKTHKAAAGYSPRLCNDDLAPTRDQNWSWYNIFSFWMSDVHSMGGYVVAASFFTLGLASWQVLLCLLVGICI
VQLCANLVAKPSQMAGVPYAVICRQAFGVFGANIPAVIRGLIAFAWYGIQTYLAANALMLVLLKFWPSLASLTSSSFLGL
SPLGWLCFATMWLLQAMVFWHGMNAIKRFIDIAGPAVYVVMLALAGWIVYKTGLDGISFTLASKSLSAGEQTWQMITATA
LVVSYFSGPLLNFGDFSRYGKSMGEIRRGNRWGLPFNFLLFSVVTVVIVSGTQSLFGKMITDPIETVSRVGNDLAVAIGL
LTMITATIGINIVANFVSPAFDFSNCAPQKISFRTGGMIAAVGSILLTPWNLFNSPELIHYTLDVLGAFIGPLFGILIAD
FYLIKRGRVSVDDLFDDTPQGKYWYRNGFNPKAIAALLPSVGLGLIISFIPALHEVANFSWFIGVFLGATTYRWLARDER
EVQAKAAFRSGAVAQKE

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=465, Percent_Identity=29.247311827957, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6322219, Length=438, Percent_Identity=22.3744292237443, Blast_Score=126, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6319495, Length=433, Percent_Identity=21.9399538106236, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319429, Length=479, Percent_Identity=22.7557411273486, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324766, Length=435, Percent_Identity=21.3793103448276, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323266, Length=439, Percent_Identity=21.6400911161731, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324645, Length=443, Percent_Identity=20.3160270880361, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 54432; Mature: 54301

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PHSSHTQQTKTHKAAAGYSPRLCNDDLAPTRDQNWSWYNIFSFWMSDVHSMGGYVVAAS
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH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HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]