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The map label for this gene is insH10 [H]

Identifier: 152970331

GI number: 152970331

Start: 1961234

End: 1962214

Strand: Direct

Name: insH10 [H]

Synonym: KPN_01779

Alternate gene names: 152970331

Gene position: 1961234-1962214 (Clockwise)

Preceding gene: 152970330

Following gene: 152970333

Centisome position: 36.9

GC content: 55.25

Gene sequence:

>981_bases
ATGAGCCATCAACTCACCTTCGCCGATAGTGAATTCAGCACTAAGCGCCGTCAGACCCGAAAAGAGATTTTCCTCTCCCG
CATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTATCCCAAGGCGGGCAATGGCCGACGGC
CCTATCCGCTGGAGACCATGCTGCGTATTCACTGCATGCAGCATTGGTACAACCTGAGCGACGGTGCCATGGAAGATGCC
CTGTACGAAATCGCCTCCATGCGCCTGTTTGCCCGATTATCCCTGGATAGCGCCCTGCCGGATCGCACCACCATCATGAA
TTTCCGCCACCTGCTCGAGCAGCATCAACTGGCCCGTCAATTGTTCAAGACCATCAATCGCTGGCTGGCCGAAGCAGGCG
TCATGATGACCCAAGGCACTTTGGTGGATGCCACCATCATTGAGGCACCCAGCTCTACCAAGAACAAAGAGCAGCAACGC
GATCCGGAGATGCATCAGACCAAGAAAGGCAATCAGTGGCACTTTGGCATGAAGGCCCACATTGGTGTCGATGCCAAGAG
TGGCCTGACCCACAGCCTAGTCACCACCGCGGCCAACGAGCATGACCTCAATCAGCTGGGTAATCTGCTTCATGGAGAGG
AGCAATTTGTCTCAGCCGATGCCGGCTACCAAGGAGCGCCACAGCGCGAGGAGCTGGCCGAGGTGGATGTGGACTGGCTG
ATCGCCGAGCGTCCCGGCAAGGTAAAAACCTTGAAGCAGCATCCGCGCAAGAACAAAACGGCCATCAACATCGAATACAT
GAAAGCCAGCATCCGTGCCAGGGTGGAGCACCCGTTTCGCATCATCAAGCGGCAGTTCGGCTTCGTGAAAGCCAGATACA
AAGGGCTGCTGAAAAACGATAACCAACTGGCGATGTTATTCACCCTGGCCAACCTGTTTCGGGTGGACCAAATGATACGT
CAGTGGGAGAGATCTCAGTAA

Upstream 100 bases:

>100_bases
TATGGATTCTGACCTAAATCATACTGCATTAGGGAAGGTGCGAACAAGTTCCTGATATGAGATCATCATATTCATCCGGA
GCGCATCCCAGAGGGACATC

Downstream 100 bases:

>100_bases
AAACCGGAAATAACGCCAGAAATGGTGGAAAAAATAGCCTAAATAGGCTGATTCGATGTGTTTGCGGGAAAAAAATCGGC
CCAGATCCGCGAAATTTTAA

Product: putative transposase, IS4

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDA
LYEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWL
IAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIR
QWERSQ

Sequences:

>Translated_326_residues
MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDA
LYEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWL
IAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIR
QWERSQ
>Mature_325_residues
SHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDAL
YEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRD
PEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLI
AERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQ
WERSQ

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: COG3039

COG function: function code L; Transposase and inactivated derivatives, IS5 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787634, Length=326, Percent_Identity=99.079754601227, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1789921, Length=325, Percent_Identity=99.0769230769231, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1789612, Length=325, Percent_Identity=99.0769230769231, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1789355, Length=325, Percent_Identity=99.0769230769231, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1788518, Length=325, Percent_Identity=99.0769230769231, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1788342, Length=325, Percent_Identity=99.0769230769231, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1787592, Length=325, Percent_Identity=99.0769230769231, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1786877, Length=325, Percent_Identity=99.0769230769231, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1786764, Length=325, Percent_Identity=99.0769230769231, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1786453, Length=325, Percent_Identity=99.0769230769231, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1788303, Length=325, Percent_Identity=98.7692307692308, Blast_Score=675, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 37857; Mature: 37726

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMVEVIEPFYPKAGNGRRPYPLETM
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHH
LRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPEMHQTKKGNQWHFGMKAH
HHHHHHHHHHHHCCEEECCCEEEHHEEECCCCCCCCHHHCCCHHHHCCCCCCEECCEEEE
IGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWL
ECCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHCCHHEE
IAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKND
EECCCCCHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NQLAMLFTLANLFRVDQMIRQWERSQ
CCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMVEVIEPFYPKAGNGRRPYPLETM
CCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHH
LRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPEMHQTKKGNQWHFGMKAH
HHHHHHHHHHHHCCEEECCCEEEHHEEECCCCCCCCHHHCCCHHHHCCCCCCEECCEEEE
IGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAGYQGAPQREELAEVDVDWL
ECCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHCCHHEE
IAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLKND
EECCCCCHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NQLAMLFTLANLFRVDQMIRQWERSQ
CCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]