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The map label for this gene is gatA2 [H]

Identifier: 152970322

GI number: 152970322

Start: 1951764

End: 1953161

Strand: Direct

Name: gatA2 [H]

Synonym: KPN_01770

Alternate gene names: 152970322

Gene position: 1951764-1953161 (Clockwise)

Preceding gene: 152970321

Following gene: 152970323

Centisome position: 36.72

GC content: 67.31

Gene sequence:

>1398_bases
ATGACTCTTCAACACTTCACCATTGCCGATCTCCAGCGTGCGCTGCACGATGGCGAACTGAGCGCCCGGGAGATCGCCCG
CCAGACGCTGGACGATATCGCCCGCGTGAATCCGCAGATCAACGCCTGGACCGAGGTCACCGCCCAGCGCATGCTGGCGG
AGGCCGACAGCATCGACGCCCTGCGCCGGGAAAAGCGCCCCCTGCCGCCGCTGGCAGGCATTCCTTACGCAGTGAAAAAT
CTGTTTGATGTCGCCGGGCATACCACCCTCGCCGGCGCCGAACTGCTGAGCGACCGGCCGCCAGCGACCAGCGACAGCTG
GGCGGTGCGCCAGCTGCACAGCGCCGGCGCCCTGCTCAGCGGGATGCTCAATATGGACGCCTACGCCTACGGCTTCACCA
CCGAAAACAGCCACTATGGCGCGACGCGCAACCCGCACGATCTGTCGCGGATCGCCGGCGGTTCTTCCGGCGGTTCGGCC
GCGGCGGTGGCGGCCGGGCTGGTTCATTTCAGCCTCGGCAGCGACACCAACGGCTCGATCCGCGTCCCCGCGTCGCTGTG
CGGGATCTTTGGCCTGAAACCCACTTTCGGGCGCCTGTCGCGCTCTGGCAGCCATCCGTTTGTCGCCAGTCTGGATCATA
TTGGTCCTTTTGCCCGCCGGGTAGCCGACCTCGCCGCGGTGTATGACGCCCTGCAGGGGCGCGACCCGGCCGATGACTTT
CAGGCTGATAAAGCCAGCGAGCGCACCGGCAATCTGCTGGAACGCGGACTGGAGGGGCTGCGCTGCGCGCGGCTGGGCGG
CTATTTCACCACCTGGTGCGATGACGACGCCCGGGCGGCCGTGGATCGGGTCGCCCACGCGCTGGGCGCCGACAGCGAGC
TGCAGTTCGCGGATGCCGCGCTGGCCCGCTCGGCGGCCTTTATCATCAGCGCCAGCGAAGGCGGCAATCAGTACCTGACG
GATCTTCGCCACTCGCCCGAGCGCTTTGAACCTCACTCCCGCGAGCGGCTGCTGGCCGGCGCGATGATCCCCTCCGCGTG
GTATCTGCAGGCTCAGCGTTTTCGCCGTCATGCCCGTCAGGCAATGAAATCCCTGTTCAGCCAGGCCGACGTGCTGATCG
CCCCGGCCACCCCATGCAGCGCCACGCCAATAGGCGCGGAGGAGATGGTGATTAACGGCCAGCCGCTGCCGGTGCGCGCC
AGCATGGGAATGCTGACGCAGCCGATCTCCTTCCTCGGGCTGCCGGTGGTGACCGTGCCCCTGCGTACCGCCAGCGGCAA
GCCGATTGGCCTGCAGCTGATTGCCGCGCCGTTTAACGAACAAGCCTGTCTTCGCGCCGCGCGGGCGCTGGAAGCGATGG
GCATTACCGATGCCCGAGTGGCGGAGAGTGCAGCATGA

Upstream 100 bases:

>100_bases
AGGAGCTGGAGCGCCAGTTTGCCCGCATCGCCGCCATGGCGCAGCCGCTGATGGACTATCCCCTCGGGCCGCGCGAGGAG
ATTGCGGGGGTGTATAAAGC

Downstream 100 bases:

>100_bases
TCAATCTGGATAATGTAGACCGCCCGGCGATCCTCGCCGAGGTAACGGCGGCGTTTTATCAGTATGAAGAGGCGCTGGTG
AGCAATAACATCGAGGCGCT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTLQHFTIADLQRALHDGELSAREIARQTLDDIARVNPQINAWTEVTAQRMLAEADSIDALRREKRPLPPLAGIPYAVKN
LFDVAGHTTLAGAELLSDRPPATSDSWAVRQLHSAGALLSGMLNMDAYAYGFTTENSHYGATRNPHDLSRIAGGSSGGSA
AAVAAGLVHFSLGSDTNGSIRVPASLCGIFGLKPTFGRLSRSGSHPFVASLDHIGPFARRVADLAAVYDALQGRDPADDF
QADKASERTGNLLERGLEGLRCARLGGYFTTWCDDDARAAVDRVAHALGADSELQFADAALARSAAFIISASEGGNQYLT
DLRHSPERFEPHSRERLLAGAMIPSAWYLQAQRFRRHARQAMKSLFSQADVLIAPATPCSATPIGAEEMVINGQPLPVRA
SMGMLTQPISFLGLPVVTVPLRTASGKPIGLQLIAAPFNEQACLRAARALEAMGITDARVAESAA

Sequences:

>Translated_465_residues
MTLQHFTIADLQRALHDGELSAREIARQTLDDIARVNPQINAWTEVTAQRMLAEADSIDALRREKRPLPPLAGIPYAVKN
LFDVAGHTTLAGAELLSDRPPATSDSWAVRQLHSAGALLSGMLNMDAYAYGFTTENSHYGATRNPHDLSRIAGGSSGGSA
AAVAAGLVHFSLGSDTNGSIRVPASLCGIFGLKPTFGRLSRSGSHPFVASLDHIGPFARRVADLAAVYDALQGRDPADDF
QADKASERTGNLLERGLEGLRCARLGGYFTTWCDDDARAAVDRVAHALGADSELQFADAALARSAAFIISASEGGNQYLT
DLRHSPERFEPHSRERLLAGAMIPSAWYLQAQRFRRHARQAMKSLFSQADVLIAPATPCSATPIGAEEMVINGQPLPVRA
SMGMLTQPISFLGLPVVTVPLRTASGKPIGLQLIAAPFNEQACLRAARALEAMGITDARVAESAA
>Mature_464_residues
TLQHFTIADLQRALHDGELSAREIARQTLDDIARVNPQINAWTEVTAQRMLAEADSIDALRREKRPLPPLAGIPYAVKNL
FDVAGHTTLAGAELLSDRPPATSDSWAVRQLHSAGALLSGMLNMDAYAYGFTTENSHYGATRNPHDLSRIAGGSSGGSAA
AVAAGLVHFSLGSDTNGSIRVPASLCGIFGLKPTFGRLSRSGSHPFVASLDHIGPFARRVADLAAVYDALQGRDPADDFQ
ADKASERTGNLLERGLEGLRCARLGGYFTTWCDDDARAAVDRVAHALGADSELQFADAALARSAAFIISASEGGNQYLTD
LRHSPERFEPHSRERLLAGAMIPSAWYLQAQRFRRHARQAMKSLFSQADVLIAPATPCSATPIGAEEMVINGQPLPVRAS
MGMLTQPISFLGLPVVTVPLRTASGKPIGLQLIAAPFNEQACLRAARALEAMGITDARVAESAA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=496, Percent_Identity=28.0241935483871, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI195972892, Length=236, Percent_Identity=30.5084745762712, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI166795287, Length=155, Percent_Identity=38.0645161290323, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=446, Percent_Identity=28.0269058295964, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=218, Percent_Identity=37.6146788990826, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17537465, Length=487, Percent_Identity=23.8193018480493, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=239, Percent_Identity=30.1255230125523, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=243, Percent_Identity=29.2181069958848, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=195, Percent_Identity=33.8461538461538, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=170, Percent_Identity=36.4705882352941, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=222, Percent_Identity=29.2792792792793, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=430, Percent_Identity=28.1395348837209, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=232, Percent_Identity=34.051724137931, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323950, Length=137, Percent_Identity=39.4160583941606, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648113, Length=493, Percent_Identity=31.6430020283976, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=26.4822134387352, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24652985, Length=493, Percent_Identity=27.789046653144, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19922090, Length=493, Percent_Identity=27.789046653144, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24652981, Length=493, Percent_Identity=27.789046653144, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24652983, Length=493, Percent_Identity=27.789046653144, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI45550774, Length=493, Percent_Identity=26.1663286004057, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24648435, Length=493, Percent_Identity=26.1663286004057, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24648437, Length=493, Percent_Identity=26.1663286004057, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI161078093, Length=499, Percent_Identity=26.25250501002, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356731, Length=203, Percent_Identity=35.4679802955665, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648441, Length=427, Percent_Identity=26.463700234192, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648439, Length=427, Percent_Identity=26.463700234192, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 49567; Mature: 49435

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQHFTIADLQRALHDGELSAREIARQTLDDIARVNPQINAWTEVTAQRMLAEADSIDA
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LRREKRPLPPLAGIPYAVKNLFDVAGHTTLAGAELLSDRPPATSDSWAVRQLHSAGALLS
HHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
GMLNMDAYAYGFTTENSHYGATRNPHDLSRIAGGSSGGSAAAVAAGLVHFSLGSDTNGSI
HHHCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCCCCCE
RVPASLCGIFGLKPTFGRLSRSGSHPFVASLDHIGPFARRVADLAAVYDALQGRDPADDF
ECCHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHH
QADKASERTGNLLERGLEGLRCARLGGYFTTWCDDDARAAVDRVAHALGADSELQFADAA
HCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHH
LARSAAFIISASEGGNQYLTDLRHSPERFEPHSRERLLAGAMIPSAWYLQAQRFRRHARQ
HHHCEEEEEEECCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AMKSLFSQADVLIAPATPCSATPIGAEEMVINGQPLPVRASMGMLTQPISFLGLPVVTVP
HHHHHHCCCCEEEECCCCCCCCCCCCHHEEECCCCCCCHHHCCHHHCCHHHHCCCEEEEE
LRTASGKPIGLQLIAAPFNEQACLRAARALEAMGITDARVAESAA
EECCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
TLQHFTIADLQRALHDGELSAREIARQTLDDIARVNPQINAWTEVTAQRMLAEADSIDA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LRREKRPLPPLAGIPYAVKNLFDVAGHTTLAGAELLSDRPPATSDSWAVRQLHSAGALLS
HHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
GMLNMDAYAYGFTTENSHYGATRNPHDLSRIAGGSSGGSAAAVAAGLVHFSLGSDTNGSI
HHHCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCCCCCE
RVPASLCGIFGLKPTFGRLSRSGSHPFVASLDHIGPFARRVADLAAVYDALQGRDPADDF
ECCHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHH
QADKASERTGNLLERGLEGLRCARLGGYFTTWCDDDARAAVDRVAHALGADSELQFADAA
HCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHH
LARSAAFIISASEGGNQYLTDLRHSPERFEPHSRERLLAGAMIPSAWYLQAQRFRRHARQ
HHHCEEEEEEECCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AMKSLFSQADVLIAPATPCSATPIGAEEMVINGQPLPVRASMGMLTQPISFLGLPVVTVP
HHHHHHCCCCEEEECCCCCCCCCCCCHHEEECCCCCCCHHHCCHHHCCHHHHCCCEEEEE
LRTASGKPIGLQLIAAPFNEQACLRAARALEAMGITDARVAESAA
EECCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]