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The map label for this gene is nepI [H]

Identifier: 152970212

GI number: 152970212

Start: 1834852

End: 1836090

Strand: Direct

Name: nepI [H]

Synonym: KPN_01660

Alternate gene names: 152970212

Gene position: 1834852-1836090 (Clockwise)

Preceding gene: 152970210

Following gene: 152970214

Centisome position: 34.52

GC content: 64.81

Gene sequence:

>1239_bases
ATGAGTTCCTGCATAGCTGCTGACGAGAGCCTGTCTCCCGCCAAACCTGCCTGGCGCGCCGTCTATGCCCTGGCCCTCGG
GGTGTTCGGCCTGATCGTGGCCGAGTTTCTGCCCGCCAGCCTGTTAACGCCGATGGCCAGCAGCCTCGGCGTCAGTGAAG
GGATGGCCGGACAGGCGGTCACCGCGACGGCGCTGGTGGCGTTAGTTACCGGCCTGCTGATCGCCACCGCCACCCGCAAT
ATCGATCGCCGCTGGGTGCTGATGTTCTTTTCCGTACTGCAGATAGTCTCCAGCCTGATGGTCGCTTTTGCCGATTCGCT
GGCGTTTCTCCTGCTGGGCCGTCTGCTGTTGGGGATCGCCATCGGCGGCTTCTGGGCGATGTCCACCGCCACGGCGATGC
GTCTGGTGCCGGCGGCCCATGTGCCAAAGGCGCTGGCGATCATCTTTTCCGCGGTGTCTGTCGCCACGGTGGTGGCCGCG
CCGCTCGGCAGCTACCTCGGGGAGCTGATCGGCTGGCGCAACGTGTTTATTCTCTGTGCGATACCCAGCCTGCTGGCGCT
GCTCTGGCAGCTGTGGGTGCTGCCTTCCATGCGCCCGGAGAGCGTCGGGACCTTCTCCACGCTGTTCAGGGTGCTGCGCC
GTCCCGGTATGCTGGGCGGCATGCTGGCGACGATCCTGATTTTCAGCGGCCATTTCGCCTTCTTTACCTACCTGCGGCCG
TTCCTTGAGACCGTCGCGCAGGCCAGTGTCGAAGGGGTTTCGCTGATATTGCTCGGCTTCGGTATCGCTAACTTTATCGG
CACTTCGGTGGCGAGCTATCTGCTGAACCGTAGCCTGCGTCTCACCCTGGCGCTGGTGCCGTTGATGATGAGCGTTCTGG
CGCTGCTGATGGTGACGTTTGGCCATCTGACCGTGCTGGCCGGGCTGCTGGTCGCCCTGTGGGGATTTGCCTTCGGCCTG
GTGCCGGTGGCCTGGTCCACCTGGCTTGCCACTACCGTGCCGGATGAAGCAGAAAGCGCGGGCGGTCTGCTGGTCGCCTC
TATTCAGCTGGCCATCAGCGCCGGGGCCGCCGGCGGCGGGGCGGTGTTTGATTTGCACGGCGCCAGCGGCGTCTTCACCG
GCAGCGGTCTGCTGCTGTTGAGCGCGATGGTGATTGTCTTCGCCGCCGTACGGGTGAAACCGGTCGCCCGCGCCGACCAG
CCGGTGACGGGCCGCTTATCCCGCGAGATCGATAACTAA

Upstream 100 bases:

>100_bases
TTCCGGACGAATAATCAATAACTGAAGAGTATGCATCATATATCGTCCGAAAGTAAGTCCCTAAGATGACCAGGATGTTA
ATAAACCTGGAGAGTTGTAT

Downstream 100 bases:

>100_bases
ATTGGCCGACAGGCTGCGGGAGCAGCACAGCGCAATATGCTGCTCTGCGGCCTGTTTTTCCGCCTCCGACTGCACGGTAT
CCCGGTGGTCGACGGTTCCC

Product: MFS family transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSSCIAADESLSPAKPAWRAVYALALGVFGLIVAEFLPASLLTPMASSLGVSEGMAGQAVTATALVALVTGLLIATATRN
IDRRWVLMFFSVLQIVSSLMVAFADSLAFLLLGRLLLGIAIGGFWAMSTATAMRLVPAAHVPKALAIIFSAVSVATVVAA
PLGSYLGELIGWRNVFILCAIPSLLALLWQLWVLPSMRPESVGTFSTLFRVLRRPGMLGGMLATILIFSGHFAFFTYLRP
FLETVAQASVEGVSLILLGFGIANFIGTSVASYLLNRSLRLTLALVPLMMSVLALLMVTFGHLTVLAGLLVALWGFAFGL
VPVAWSTWLATTVPDEAESAGGLLVASIQLAISAGAAGGGAVFDLHGASGVFTGSGLLLLSAMVIVFAAVRVKPVARADQ
PVTGRLSREIDN

Sequences:

>Translated_412_residues
MSSCIAADESLSPAKPAWRAVYALALGVFGLIVAEFLPASLLTPMASSLGVSEGMAGQAVTATALVALVTGLLIATATRN
IDRRWVLMFFSVLQIVSSLMVAFADSLAFLLLGRLLLGIAIGGFWAMSTATAMRLVPAAHVPKALAIIFSAVSVATVVAA
PLGSYLGELIGWRNVFILCAIPSLLALLWQLWVLPSMRPESVGTFSTLFRVLRRPGMLGGMLATILIFSGHFAFFTYLRP
FLETVAQASVEGVSLILLGFGIANFIGTSVASYLLNRSLRLTLALVPLMMSVLALLMVTFGHLTVLAGLLVALWGFAFGL
VPVAWSTWLATTVPDEAESAGGLLVASIQLAISAGAAGGGAVFDLHGASGVFTGSGLLLLSAMVIVFAAVRVKPVARADQ
PVTGRLSREIDN
>Mature_411_residues
SSCIAADESLSPAKPAWRAVYALALGVFGLIVAEFLPASLLTPMASSLGVSEGMAGQAVTATALVALVTGLLIATATRNI
DRRWVLMFFSVLQIVSSLMVAFADSLAFLLLGRLLLGIAIGGFWAMSTATAMRLVPAAHVPKALAIIFSAVSVATVVAAP
LGSYLGELIGWRNVFILCAIPSLLALLWQLWVLPSMRPESVGTFSTLFRVLRRPGMLGGMLATILIFSGHFAFFTYLRPF
LETVAQASVEGVSLILLGFGIANFIGTSVASYLLNRSLRLTLALVPLMMSVLALLMVTFGHLTVLAGLLVALWGFAFGLV
PVAWSTWLATTVPDEAESAGGLLVASIQLAISAGAAGGGAVFDLHGASGVFTGSGLLLLSAMVIVFAAVRVKPVARADQP
VTGRLSREIDN

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=394, Percent_Identity=48.7309644670051, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1786595, Length=282, Percent_Identity=28.7234042553192, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1787947, Length=310, Percent_Identity=29.6774193548387, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787808, Length=352, Percent_Identity=27.2727272727273, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43035; Mature: 42904

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSCIAADESLSPAKPAWRAVYALALGVFGLIVAEFLPASLLTPMASSLGVSEGMAGQAV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
TATALVALVTGLLIATATRNIDRRWVLMFFSVLQIVSSLMVAFADSLAFLLLGRLLLGIA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGGFWAMSTATAMRLVPAAHVPKALAIIFSAVSVATVVAAPLGSYLGELIGWRNVFILCA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPSLLALLWQLWVLPSMRPESVGTFSTLFRVLRRPGMLGGMLATILIFSGHFAFFTYLRP
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FLETVAQASVEGVSLILLGFGIANFIGTSVASYLLNRSLRLTLALVPLMMSVLALLMVTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GHLTVLAGLLVALWGFAFGLVPVAWSTWLATTVPDEAESAGGLLVASIQLAISAGAAGGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEHHHHHHHHHCCCCCCC
AVFDLHGASGVFTGSGLLLLSAMVIVFAAVRVKPVARADQPVTGRLSREIDN
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SSCIAADESLSPAKPAWRAVYALALGVFGLIVAEFLPASLLTPMASSLGVSEGMAGQAV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
TATALVALVTGLLIATATRNIDRRWVLMFFSVLQIVSSLMVAFADSLAFLLLGRLLLGIA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGGFWAMSTATAMRLVPAAHVPKALAIIFSAVSVATVVAAPLGSYLGELIGWRNVFILCA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPSLLALLWQLWVLPSMRPESVGTFSTLFRVLRRPGMLGGMLATILIFSGHFAFFTYLRP
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
FLETVAQASVEGVSLILLGFGIANFIGTSVASYLLNRSLRLTLALVPLMMSVLALLMVTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GHLTVLAGLLVALWGFAFGLVPVAWSTWLATTVPDEAESAGGLLVASIQLAISAGAAGGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEHHHHHHHHHCCCCCCC
AVFDLHGASGVFTGSGLLLLSAMVIVFAAVRVKPVARADQPVTGRLSREIDN
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA