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The map label for this gene is ydfI [H]

Identifier: 152970139

GI number: 152970139

Start: 1763192

End: 1764655

Strand: Direct

Name: ydfI [H]

Synonym: KPN_01587

Alternate gene names: 152970139

Gene position: 1763192-1764655 (Clockwise)

Preceding gene: 152970138

Following gene: 152970140

Centisome position: 33.17

GC content: 60.38

Gene sequence:

>1464_bases
ATGGAAAACACCCTCTTAACCGCTAACGCCACGCTGCCGACCTACGATCGCAGCGCACTGATCCCCCGTATCGTTCATCT
TGGTTTTGGCGCGTTTCATCGCGCTCATCAGGCTGTGTATGCCGATATACTGGCCAGCGAGCACGGCAGCGACTGGGGCT
ACACCGAAGTTAATCTGATTGGCGGCGAGCAGCAGATCGCCGATCTCCAGCAGCAGGATCTGCTGTATACCGTCGCCGAA
ATGTCCGCCGACGCCTGGACCGCGCGCGTGGTTGGTGTGGTGAAGAAGGCGCTGCACGCCGGGGTTGATGGTCTCGAAGC
GGTGCTGGCGGCGATGTGTCAGCCACAGGTGGCCATTGTCTCCCTGACGATTACTGAGAAAGGCTATTGCCATTCGCCAG
CCAGCGGCGAACTGCAGCTCGATCACCCGCTGATCGCCGCCGATTTGCATAACCCGCACCAGCCAAAATCGGCGGCGGGG
GTAGTGGTGGAGGCCTTATCTCGCCGCCGGGCGGCCGGCCTGCCTGCTTTTACCGTGATGTCCTGCGATAACATGCCGGA
AAATGGTCACGTGATGCGCAACGTCGTCTGCGCCTACGCCCGGGCTCTGGACGAGGATCTTGCCGCATGGATAGAGCAGA
ATGTCACCTTTCCGTCGACCATGGTGGACCGGATTGTCCCGGCGGTGACTGCCGAAACCCTCGATAAAATTACCCAGCTG
ACCGGGGTGCGCGACCCGGCCGGGGTGGCCTGCGAACCTTTCCGTCAGTGGGTGATTGAGGATAACTTTGTTGCCGGACG
TCCGCAGTGGGAGAAAGCGGGAGCGGAACTGGTCACCGACGTGGTGCCTTTCGAAGAGATGAAGCTGAGAATGCTCAATG
GTAGCCATTCGTTCCTCGCTTATCTCGGCTATCTGGCCGGTTATCAGCATATCAACGACTGTATGCAGGACGATAACTAC
CGTCGCGCGGCGCTGTCGTTGATGCTGGACGAACAAGCACCAACGCTGAAGGTGCAGGGGGTGGATCTGTCACGCTATGC
CAGTTTGCTGATTGACCGTTACTGCAATCCGGCGCTGAAGCACCGCACCTGGCAGATAGCCATGGACGGCAGCCAGAAGT
TGCCCCAGCGCATGCTGGACTCCATACGCTGGCATCTGGTGCATCAGCGTGATTTTACACTGCTGGCGCTGGGCGTGGCA
GGGTGGATGCGTTACGTTGGCGGCGTTGACGATGCGGGCCAGTCAATCGAGATATGCGACCCGTTGTTGCCGGTTATCCA
GCAAGCGGTGGCTGCCAGCGCCGATGGCGAAGCGCGAGTCAAAGCGCTGCTGGGTATTGAGGCTATTTTCGGCGTCGAAC
TGCCGCAGGAGTCACGCTTTGTCACGGCGGTGACCCGCGCCTACTTAGCGCTGCAGCGGCAGGGGGCTAAAGCTACCGTC
GCCGCGTGGGCCGCCGCGCAATAA

Upstream 100 bases:

>100_bases
ATGTTATACCAATTGCGTGAGATGGTTGAGGCACAAAGGCATTCTCCTCGAGAGTTCTCCCTGAGAATGGTTGATGGGCT
ACATAACAACGAGAGTCATC

Downstream 100 bases:

>100_bases
TCTTTTTCCGGTGTCGGTCCCGGGGTCGACACCGGCTCTTCAGTTATTTCTACGATACTCCGCTGATGTCTATTTTTTGC
TAGCGATCGGTTTTAGCAGA

Product: putative mannitol dehydrogenase

Products: D-fructuronate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MENTLLTANATLPTYDRSALIPRIVHLGFGAFHRAHQAVYADILASEHGSDWGYTEVNLIGGEQQIADLQQQDLLYTVAE
MSADAWTARVVGVVKKALHAGVDGLEAVLAAMCQPQVAIVSLTITEKGYCHSPASGELQLDHPLIAADLHNPHQPKSAAG
VVVEALSRRRAAGLPAFTVMSCDNMPENGHVMRNVVCAYARALDEDLAAWIEQNVTFPSTMVDRIVPAVTAETLDKITQL
TGVRDPAGVACEPFRQWVIEDNFVAGRPQWEKAGAELVTDVVPFEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMQDDNY
RRAALSLMLDEQAPTLKVQGVDLSRYASLLIDRYCNPALKHRTWQIAMDGSQKLPQRMLDSIRWHLVHQRDFTLLALGVA
GWMRYVGGVDDAGQSIEICDPLLPVIQQAVAASADGEARVKALLGIEAIFGVELPQESRFVTAVTRAYLALQRQGAKATV
AAWAAAQ

Sequences:

>Translated_487_residues
MENTLLTANATLPTYDRSALIPRIVHLGFGAFHRAHQAVYADILASEHGSDWGYTEVNLIGGEQQIADLQQQDLLYTVAE
MSADAWTARVVGVVKKALHAGVDGLEAVLAAMCQPQVAIVSLTITEKGYCHSPASGELQLDHPLIAADLHNPHQPKSAAG
VVVEALSRRRAAGLPAFTVMSCDNMPENGHVMRNVVCAYARALDEDLAAWIEQNVTFPSTMVDRIVPAVTAETLDKITQL
TGVRDPAGVACEPFRQWVIEDNFVAGRPQWEKAGAELVTDVVPFEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMQDDNY
RRAALSLMLDEQAPTLKVQGVDLSRYASLLIDRYCNPALKHRTWQIAMDGSQKLPQRMLDSIRWHLVHQRDFTLLALGVA
GWMRYVGGVDDAGQSIEICDPLLPVIQQAVAASADGEARVKALLGIEAIFGVELPQESRFVTAVTRAYLALQRQGAKATV
AAWAAAQ
>Mature_487_residues
MENTLLTANATLPTYDRSALIPRIVHLGFGAFHRAHQAVYADILASEHGSDWGYTEVNLIGGEQQIADLQQQDLLYTVAE
MSADAWTARVVGVVKKALHAGVDGLEAVLAAMCQPQVAIVSLTITEKGYCHSPASGELQLDHPLIAADLHNPHQPKSAAG
VVVEALSRRRAAGLPAFTVMSCDNMPENGHVMRNVVCAYARALDEDLAAWIEQNVTFPSTMVDRIVPAVTAETLDKITQL
TGVRDPAGVACEPFRQWVIEDNFVAGRPQWEKAGAELVTDVVPFEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMQDDNY
RRAALSLMLDEQAPTLKVQGVDLSRYASLLIDRYCNPALKHRTWQIAMDGSQKLPQRMLDSIRWHLVHQRDFTLLALGVA
GWMRYVGGVDDAGQSIEICDPLLPVIQQAVAASADGEARVKALLGIEAIFGVELPQESRFVTAVTRAYLALQRQGAKATV
AAWAAAQ

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787823, Length=484, Percent_Identity=78.9256198347107, Blast_Score=804, Evalue=0.0,
Organism=Escherichia coli, GI1790779, Length=473, Percent_Identity=59.830866807611, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI1788497, Length=464, Percent_Identity=57.9741379310345, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI1790028, Length=255, Percent_Identity=28.6274509803922, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994885, Length=367, Percent_Identity=25.8855585831063, Blast_Score=102, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324401, Length=485, Percent_Identity=40.8247422680412, Blast_Score=352, Evalue=6e-98,
Organism=Saccharomyces cerevisiae, GI6320765, Length=485, Percent_Identity=40.8247422680412, Blast_Score=352, Evalue=6e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53214; Mature: 53214

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTLLTANATLPTYDRSALIPRIVHLGFGAFHRAHQAVYADILASEHGSDWGYTEVNLI
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
GGEQQIADLQQQDLLYTVAEMSADAWTARVVGVVKKALHAGVDGLEAVLAAMCQPQVAIV
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEE
SLTITEKGYCHSPASGELQLDHPLIAADLHNPHQPKSAAGVVVEALSRRRAAGLPAFTVM
EEEEECCCCCCCCCCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
SCDNMPENGHVMRNVVCAYARALDEDLAAWIEQNVTFPSTMVDRIVPAVTAETLDKITQL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TGVRDPAGVACEPFRQWVIEDNFVAGRPQWEKAGAELVTDVVPFEEMKLRMLNGSHSFLA
HCCCCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHH
YLGYLAGYQHINDCMQDDNYRRAALSLMLDEQAPTLKVQGVDLSRYASLLIDRYCNPALK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCHHHH
HRTWQIAMDGSQKLPQRMLDSIRWHLVHQRDFTLLALGVAGWMRYVGGVDDAGQSIEICD
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHH
PLLPVIQQAVAASADGEARVKALLGIEAIFGVELPQESRFVTAVTRAYLALQRQGAKATV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEH
AAWAAAQ
HHHHCCC
>Mature Secondary Structure
MENTLLTANATLPTYDRSALIPRIVHLGFGAFHRAHQAVYADILASEHGSDWGYTEVNLI
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
GGEQQIADLQQQDLLYTVAEMSADAWTARVVGVVKKALHAGVDGLEAVLAAMCQPQVAIV
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEE
SLTITEKGYCHSPASGELQLDHPLIAADLHNPHQPKSAAGVVVEALSRRRAAGLPAFTVM
EEEEECCCCCCCCCCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
SCDNMPENGHVMRNVVCAYARALDEDLAAWIEQNVTFPSTMVDRIVPAVTAETLDKITQL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TGVRDPAGVACEPFRQWVIEDNFVAGRPQWEKAGAELVTDVVPFEEMKLRMLNGSHSFLA
HCCCCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHH
YLGYLAGYQHINDCMQDDNYRRAALSLMLDEQAPTLKVQGVDLSRYASLLIDRYCNPALK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCHHHH
HRTWQIAMDGSQKLPQRMLDSIRWHLVHQRDFTLLALGVAGWMRYVGGVDDAGQSIEICD
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHH
PLLPVIQQAVAASADGEARVKALLGIEAIFGVELPQESRFVTAVTRAYLALQRQGAKATV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEH
AAWAAAQ
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-mannonate; NAD(+) [C]

Specific reaction: D-mannonate + NAD(+) = D-fructuronate + NADH [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]