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The map label for this gene is bioD [H]

Identifier: 152970127

GI number: 152970127

Start: 1752215

End: 1752910

Strand: Direct

Name: bioD [H]

Synonym: KPN_01575

Alternate gene names: 152970127

Gene position: 1752215-1752910 (Clockwise)

Preceding gene: 152970126

Following gene: 152970134

Centisome position: 32.97

GC content: 59.77

Gene sequence:

>696_bases
ATGTTTAAGCGTTTCTTTATTACAGGTACAGACACCTCTGTCGGGAAGACAGTGGTTTCCCGTGCGCTGTTGCAGGCACT
TGCTGCCAGAGGCAAAAGCGTGGCCGGGTATAAGCCTGTGGCGAAGGGCAGCAAAGAGACGCCGGACGGTTTACGTAACA
AAGATGCGCTGGTGTTGCAGAGCGTCTCCACGCTGGCCTTACCCTATGAGGCTGTCAACCCCATCGCCTTAAGCGAAGAC
GAGAGCAGCGTGGCGCATAGCTGCCCGATCAACTACGGTCTGCTGTCCGATGGTTTGCAGCGTCTGGCCGCGCAGGTCGA
ACACGTGGTGGTTGAAGGCACCGGCGGCTGGCGCAGCCTGATGAACGACCTGCGGCCGCTCTCCGAGTGGGTGGTTCAGG
AGCAGCTCCCAGTGCTGATGGTGGTGGGGATCCAGGAGGGGTGCATCAACCATGCGCTACTGACAGCCCAGGCGATCGCC
AATGACGGTTTACCGCTGATTGGCTGGGTGGCCAATCGGATCAACCCCGGTCTGGCGCACTACGCCGAAATCATCGATGT
CCTCAGCAAAAAGCTGCCGGCGCCGCTGATCGGCGAGCTGCCTTATCTACCGCGCGCCGAACAGCGTGAGCTGAGCCGCT
ACGTCGACTTAGACATGCTCGGCAACGTGATGGCGATAGATCGAATCCCGGCGTAA

Upstream 100 bases:

>100_bases
GCGCTATCTCAAGCTGACCTCAGACGGCTTCACGTAGACTTTGCCCACCGTTGATTATTTCTCTCCCGTATATTTCATCG
ACAAAACTGTGGGGTTAGTG

Downstream 100 bases:

>100_bases
CGTCCGCGACAGCACAGACGACACGACGCAGGTTATCAGCAGACCGGGCAGCAGCGTATACTGCCCGGTCATCTCGCAAA
TCATCAGGGTTGACATGATC

Product: putative dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MFKRFFITGTDTSVGKTVVSRALLQALAARGKSVAGYKPVAKGSKETPDGLRNKDALVLQSVSTLALPYEAVNPIALSED
ESSVAHSCPINYGLLSDGLQRLAAQVEHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
NDGLPLIGWVANRINPGLAHYAEIIDVLSKKLPAPLIGELPYLPRAEQRELSRYVDLDMLGNVMAIDRIPA

Sequences:

>Translated_231_residues
MFKRFFITGTDTSVGKTVVSRALLQALAARGKSVAGYKPVAKGSKETPDGLRNKDALVLQSVSTLALPYEAVNPIALSED
ESSVAHSCPINYGLLSDGLQRLAAQVEHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
NDGLPLIGWVANRINPGLAHYAEIIDVLSKKLPAPLIGELPYLPRAEQRELSRYVDLDMLGNVMAIDRIPA
>Mature_231_residues
MFKRFFITGTDTSVGKTVVSRALLQALAARGKSVAGYKPVAKGSKETPDGLRNKDALVLQSVSTLALPYEAVNPIALSED
ESSVAHSCPINYGLLSDGLQRLAAQVEHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
NDGLPLIGWVANRINPGLAHYAEIIDVLSKKLPAPLIGELPYLPRAEQRELSRYVDLDMLGNVMAIDRIPA

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081944, Length=229, Percent_Identity=87.7729257641921, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=50.2222222222222, Blast_Score=219, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 24928; Mature: 24928

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRFFITGTDTSVGKTVVSRALLQALAARGKSVAGYKPVAKGSKETPDGLRNKDALVLQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
SVSTLALPYEAVNPIALSEDESSVAHSCPINYGLLSDGLQRLAAQVEHVVVEGTGGWRSL
HHHHHHCCHHHCCCCEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHH
MNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIANDGLPLIGWVANRINPGLAH
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
YAEIIDVLSKKLPAPLIGELPYLPRAEQRELSRYVDLDMLGNVMAIDRIPA
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHCHHHHCCCHHHCCCCC
>Mature Secondary Structure
MFKRFFITGTDTSVGKTVVSRALLQALAARGKSVAGYKPVAKGSKETPDGLRNKDALVLQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
SVSTLALPYEAVNPIALSEDESSVAHSCPINYGLLSDGLQRLAAQVEHVVVEGTGGWRSL
HHHHHHCCHHHCCCCEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHH
MNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIANDGLPLIGWVANRINPGLAH
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
YAEIIDVLSKKLPAPLIGELPYLPRAEQRELSRYVDLDMLGNVMAIDRIPA
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHCHHHHCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]