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The map label for this gene is mlc [H]

Identifier: 152970126

GI number: 152970126

Start: 1750864

End: 1752087

Strand: Direct

Name: mlc [H]

Synonym: KPN_01574

Alternate gene names: 152970126

Gene position: 1750864-1752087 (Clockwise)

Preceding gene: 152970125

Following gene: 152970127

Centisome position: 32.94

GC content: 57.11

Gene sequence:

>1224_bases
ATGGTGGTAGCGGATAGTCAACCAGGCCATATCGATCAAATAAAGCAGACCAATGCGGGCGCCGTGTATCGCCTGATTGA
TCAGCTCGGCCCGGTATCGCGTATCGACCTTTCCCGATTTGCGCAACTGGCGCCGGCCAGCATCACCAAAATCGTCCGCG
AAATGCTGGAAGCGCATCTGGTTCAGGAGACCGAGATCCAGGAGCCCGGCAGCCGCGGCCGCCCGGCGGTGGGGCTGATG
GTGGAGACCGAGGCATGGCACTATCTGGCGGTGCGCATCAGCCGCGGTGAGATCCATCTTTCGCTGCGTGATTTAAGCAG
TCAGCTGGTGGTGGAGGATCAGCTGGAGCTGGCGCTCACCGACAGCACGCCGTTTCTGACGCGCGTTATCGACCATATTG
ATCGCTTCTTTATTCGCCATCAAAAGAAGCTGGAACGCCTTACCTCCATCGCCATGACGATGCCCGGTATTATCGATACC
GAAAATGGCATTATTCATCGCATGCCGTTTTATGAGGATGTTAAAGATGTCCCGCTCGGCGAAGCGCTGGCCAATCATAC
CGGGGTGCCAGTCTATATTCAGCATGACATCAGCGCCTGGACCATGGCGGAATCTTTGTTTGGCGCCTCGCGCGGCGCCC
GGGACGTCATTCAGGTGGTCATCGATCATAACGTCGGCGCCGGGGTGATCACCGATGGGCGCCTGCTGCACGCCGGCAGC
AGCAGCCTGGTGGAGATTGGCCATACCCAGGTCGATCCCTACGGCAAACGCTGTTACTGCGGCAACCATGGTTGCCTGGA
GACAATTGCCAGCGTAGAAAGCGTGCTGGAGCTGGCGCAGATGCGCATGGCGCAGTCGATGAGCTCGCTGCTGCACCAGC
GTCCGCTGAGCGTGGAGTGGCTTTGTCAGGCGGCGCTGCAGGGCGATTTGTTAGCCCGGGACATCATCAGCGGCGTGGGT
AATCACGTTGGGCGGATTCTGGCGATCATGGTCAATCTGTTTAATCCGCAGAAAATTCTGATTGGCTCGCCGTTCAATCT
GGCGGCAGAAATTCTTTTTCCGGCAATTTCCAGCTGTATTCGGCAACAATCTCTCCCAGCCTATAGCCGGCATATTACCG
TTGAAAGTACTCAGTTCTCCAACCGCGGAACGATGGCTGGCGCGGCGCTGGTTAAAGATGCGCTCTATAACGGATCCTTA
CTCATACGACTGTTACAGGGTTAA

Upstream 100 bases:

>100_bases
GTGCGGTAAATCACAAGGCTAAGTAAAAATTTGACGTTCACCGTCGAAGTGCTTCACCATAGCCGAAAGTTTATTTCGAA
GCGCGAAAATAAGGGAGTCA

Downstream 100 bases:

>100_bases
CATTTTTTCATAGTCACACCAAAAATTGCGCTATCTCAAGCTGACCTCAGACGGCTTCACGTAGACTTTGCCCACCGTTG
ATTATTTCTCTCCCGTATAT

Product: putative NAGC-like transcriptional regulator

Products: NA

Alternate protein names: Making large colonies protein [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MVVADSQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRFAQLAPASITKIVREMLEAHLVQETEIQEPGSRGRPAVGLM
VETEAWHYLAVRISRGEIHLSLRDLSSQLVVEDQLELALTDSTPFLTRVIDHIDRFFIRHQKKLERLTSIAMTMPGIIDT
ENGIIHRMPFYEDVKDVPLGEALANHTGVPVYIQHDISAWTMAESLFGASRGARDVIQVVIDHNVGAGVITDGRLLHAGS
SSLVEIGHTQVDPYGKRCYCGNHGCLETIASVESVLELAQMRMAQSMSSLLHQRPLSVEWLCQAALQGDLLARDIISGVG
NHVGRILAIMVNLFNPQKILIGSPFNLAAEILFPAISSCIRQQSLPAYSRHITVESTQFSNRGTMAGAALVKDALYNGSL
LIRLLQG

Sequences:

>Translated_407_residues
MVVADSQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRFAQLAPASITKIVREMLEAHLVQETEIQEPGSRGRPAVGLM
VETEAWHYLAVRISRGEIHLSLRDLSSQLVVEDQLELALTDSTPFLTRVIDHIDRFFIRHQKKLERLTSIAMTMPGIIDT
ENGIIHRMPFYEDVKDVPLGEALANHTGVPVYIQHDISAWTMAESLFGASRGARDVIQVVIDHNVGAGVITDGRLLHAGS
SSLVEIGHTQVDPYGKRCYCGNHGCLETIASVESVLELAQMRMAQSMSSLLHQRPLSVEWLCQAALQGDLLARDIISGVG
NHVGRILAIMVNLFNPQKILIGSPFNLAAEILFPAISSCIRQQSLPAYSRHITVESTQFSNRGTMAGAALVKDALYNGSL
LIRLLQG
>Mature_407_residues
MVVADSQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRFAQLAPASITKIVREMLEAHLVQETEIQEPGSRGRPAVGLM
VETEAWHYLAVRISRGEIHLSLRDLSSQLVVEDQLELALTDSTPFLTRVIDHIDRFFIRHQKKLERLTSIAMTMPGIIDT
ENGIIHRMPFYEDVKDVPLGEALANHTGVPVYIQHDISAWTMAESLFGASRGARDVIQVVIDHNVGAGVITDGRLLHAGS
SSLVEIGHTQVDPYGKRCYCGNHGCLETIASVESVLELAQMRMAQSMSSLLHQRPLSVEWLCQAALQGDLLARDIISGVG
NHVGRILAIMVNLFNPQKILIGSPFNLAAEILFPAISSCIRQQSLPAYSRHITVESTQFSNRGTMAGAALVKDALYNGSL
LIRLLQG

Specific function: Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI4885285, Length=231, Percent_Identity=25.974025974026, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI190014632, Length=231, Percent_Identity=25.974025974026, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI298566315, Length=231, Percent_Identity=25.974025974026, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI298566325, Length=231, Percent_Identity=25.974025974026, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1787878, Length=406, Percent_Identity=84.9753694581281, Blast_Score=719, Evalue=0.0,
Organism=Escherichia coli, GI1786891, Length=401, Percent_Identity=39.1521197007481, Blast_Score=304, Evalue=8e-84,
Organism=Escherichia coli, GI87082127, Length=371, Percent_Identity=22.1024258760108, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: NA

Molecular weight: Translated: 44668; Mature: 44668

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVADSQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRFAQLAPASITKIVREMLEAHL
CEECCCCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHH
VQETEIQEPGSRGRPAVGLMVETEAWHYLAVRISRGEIHLSLRDLSSQLVVEDQLELALT
HHHHHCCCCCCCCCCEEEEEEECCCEEEEEEEEECCEEEEEHHHHCCHHEEECCEEEEEE
DSTPFLTRVIDHIDRFFIRHQKKLERLTSIAMTMPGIIDTENGIIHRMPFYEDVKDVPLG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEECCCHHHHHCCCCH
EALANHTGVPVYIQHDISAWTMAESLFGASRGARDVIQVVIDHNVGAGVITDGRLLHAGS
HHHHCCCCCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEECCEEEECCC
SSLVEIGHTQVDPYGKRCYCGNHGCLETIASVESVLELAQMRMAQSMSSLLHQRPLSVEW
CCCEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LCQAALQGDLLARDIISGVGNHVGRILAIMVNLFNPQKILIGSPFNLAAEILFPAISSCI
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHH
RQQSLPAYSRHITVESTQFSNRGTMAGAALVKDALYNGSLLIRLLQG
HHHCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHEEECC
>Mature Secondary Structure
MVVADSQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRFAQLAPASITKIVREMLEAHL
CEECCCCCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHH
VQETEIQEPGSRGRPAVGLMVETEAWHYLAVRISRGEIHLSLRDLSSQLVVEDQLELALT
HHHHHCCCCCCCCCCEEEEEEECCCEEEEEEEEECCEEEEEHHHHCCHHEEECCEEEEEE
DSTPFLTRVIDHIDRFFIRHQKKLERLTSIAMTMPGIIDTENGIIHRMPFYEDVKDVPLG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEECCCHHHHHCCCCH
EALANHTGVPVYIQHDISAWTMAESLFGASRGARDVIQVVIDHNVGAGVITDGRLLHAGS
HHHHCCCCCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEECCEEEECCC
SSLVEIGHTQVDPYGKRCYCGNHGCLETIASVESVLELAQMRMAQSMSSLLHQRPLSVEW
CCCEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LCQAALQGDLLARDIISGVGNHVGRILAIMVNLFNPQKILIGSPFNLAAEILFPAISSCI
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHH
RQQSLPAYSRHITVESTQFSNRGTMAGAALVKDALYNGSLLIRLLQG
HHHCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766024; 9097039; 9278503 [H]