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The map label for this gene is ynfL [H]

Identifier: 152970125

GI number: 152970125

Start: 1749841

End: 1750743

Strand: Direct

Name: ynfL [H]

Synonym: KPN_01573

Alternate gene names: 152970125

Gene position: 1749841-1750743 (Clockwise)

Preceding gene: 152970123

Following gene: 152970126

Centisome position: 32.92

GC content: 61.68

Gene sequence:

>903_bases
ATGAATATCGAGTTGCGTCATTTGCGCTACTTTGTCGCCGTCGCTGAGGAGCTACATTTTGGCCGCGCCGCGGCGCGACT
GAACATCTCTCAACCACCGCTAAGCCAGCAGATTCAGATCCTTGAGCAGCAGACCGGCGCTCGCCTGTTTGCCCGTACCA
ACCGCAGCGTCAGCCTGACGGCGGCCGGAAAGCAGTTTCTCGCCGATAGCCGACAAATCCTCGCCCAGGTGGATGAGGCG
GCTGCTCGCGCCGCCCGTCTACACCATGGCGAAACGGGTGAGCTGCGCATCGGCTTTACCTCCTCGGCCCCTTTTATTAA
GGCCGTCTCCGATACGCTATCGATGTTTCGCCAACGCCTTCCGGATGTGCATATTCTCACCCGCGAGACTAACACCCGCG
AACAGATAGTGCCCCTGAGCGAAGGGGCTCTGGATTTGGGGCTGCTGCGAAATACTCAACTGCCGGACACTCTGGCGTGG
GAGCGGGTTCTGCGCGAGCCGCTGTTGGCGATGGTCCCGGCGAATCACCCGCTGGCCCGCCAGCCCTCGGTCAGCCTGGC
GGCGCTGGCGCGCGAGCCCTTCGTCTTTTTCGACCCGCACGTCGGCACTGGCCTGTACGACGACATCCTCGGCTTGCTGC
GCCGCTACGGCCACACGCCGAAGATTGCCCAGGAGGTGGGGGAGGCAATGACGATTATTGGTCTGGTGGCCGCCGGACTG
GGGGTATCGATCCTCCCGGCCTCCTTTCAGCGCGTCCAGCTCAGTGAAATGCGCTGGCTGCCGATCGACGAACAGGATGC
GGTATCGGAGATGTGGCTGGTGTGGTCTAAACACCATGAGCAAGGGGCACTGGCAAAACGCTTTCGTGAGGCGCTGCTCT
CCTGGAAAAGCGAGCATAATTGA

Upstream 100 bases:

>100_bases
ACCAACAGAGTGTTGTGACCATTCAGCTCACATTTTTAGGGTATGAAAATGTAAATATTCTGTCTAATATATTAATAATC
TCAAATAAGACGTTTTAAAT

Downstream 100 bases:

>100_bases
GGCGAAAAGGGGCTGGAAATGTGCGGTAAATCACAAGGCTAAGTAAAAATTTGACGTTCACCGTCGAAGTGCTTCACCAT
AGCCGAAAGTTTATTTCGAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQTGARLFARTNRSVSLTAAGKQFLADSRQILAQVDEA
AARAARLHHGETGELRIGFTSSAPFIKAVSDTLSMFRQRLPDVHILTRETNTREQIVPLSEGALDLGLLRNTQLPDTLAW
ERVLREPLLAMVPANHPLARQPSVSLAALAREPFVFFDPHVGTGLYDDILGLLRRYGHTPKIAQEVGEAMTIIGLVAAGL
GVSILPASFQRVQLSEMRWLPIDEQDAVSEMWLVWSKHHEQGALAKRFREALLSWKSEHN

Sequences:

>Translated_300_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQTGARLFARTNRSVSLTAAGKQFLADSRQILAQVDEA
AARAARLHHGETGELRIGFTSSAPFIKAVSDTLSMFRQRLPDVHILTRETNTREQIVPLSEGALDLGLLRNTQLPDTLAW
ERVLREPLLAMVPANHPLARQPSVSLAALAREPFVFFDPHVGTGLYDDILGLLRRYGHTPKIAQEVGEAMTIIGLVAAGL
GVSILPASFQRVQLSEMRWLPIDEQDAVSEMWLVWSKHHEQGALAKRFREALLSWKSEHN
>Mature_300_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQTGARLFARTNRSVSLTAAGKQFLADSRQILAQVDEA
AARAARLHHGETGELRIGFTSSAPFIKAVSDTLSMFRQRLPDVHILTRETNTREQIVPLSEGALDLGLLRNTQLPDTLAW
ERVLREPLLAMVPANHPLARQPSVSLAALAREPFVFFDPHVGTGLYDDILGLLRRYGHTPKIAQEVGEAMTIIGLVAAGL
GVSILPASFQRVQLSEMRWLPIDEQDAVSEMWLVWSKHHEQGALAKRFREALLSWKSEHN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=294, Percent_Identity=78.9115646258503, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1788887, Length=282, Percent_Identity=35.4609929078014, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI2367136, Length=284, Percent_Identity=34.5070422535211, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1790399, Length=248, Percent_Identity=31.0483870967742, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788748, Length=249, Percent_Identity=30.1204819277108, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI145693105, Length=252, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI157672245, Length=249, Percent_Identity=30.5220883534137, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=30.0813008130081, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787530, Length=276, Percent_Identity=25.3623188405797, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1789506, Length=282, Percent_Identity=24.113475177305, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786713, Length=269, Percent_Identity=28.2527881040892, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787601, Length=253, Percent_Identity=29.2490118577075, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790262, Length=253, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787806, Length=279, Percent_Identity=25.4480286738351, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=94, Percent_Identity=42.5531914893617, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788481, Length=299, Percent_Identity=26.4214046822742, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33519; Mature: 33519

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQTGARLFARTNRSVSLT
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCEEEECCCCEEEE
AAGKQFLADSRQILAQVDEAAARAARLHHGETGELRIGFTSSAPFIKAVSDTLSMFRQRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHC
PDVHILTRETNTREQIVPLSEGALDLGLLRNTQLPDTLAWERVLREPLLAMVPANHPLAR
CCEEEEECCCCCHHHEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCCCC
QPSVSLAALAREPFVFFDPHVGTGLYDDILGLLRRYGHTPKIAQEVGEAMTIIGLVAAGL
CCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
GVSILPASFQRVQLSEMRWLPIDEQDAVSEMWLVWSKHHEQGALAKRFREALLSWKSEHN
CCCHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQTGARLFARTNRSVSLT
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCEEEECCCCEEEE
AAGKQFLADSRQILAQVDEAAARAARLHHGETGELRIGFTSSAPFIKAVSDTLSMFRQRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHC
PDVHILTRETNTREQIVPLSEGALDLGLLRNTQLPDTLAWERVLREPLLAMVPANHPLAR
CCEEEEECCCCCHHHEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCCCC
QPSVSLAALAREPFVFFDPHVGTGLYDDILGLLRRYGHTPKIAQEVGEAMTIIGLVAAGL
CCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
GVSILPASFQRVQLSEMRWLPIDEQDAVSEMWLVWSKHHEQGALAKRFREALLSWKSEHN
CCCHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]