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The map label for this gene is yogA [H]

Identifier: 152969913

GI number: 152969913

Start: 1525836

End: 1526870

Strand: Direct

Name: yogA [H]

Synonym: KPN_01360

Alternate gene names: 152969913

Gene position: 1525836-1526870 (Clockwise)

Preceding gene: 152969911

Following gene: 152969915

Centisome position: 28.71

GC content: 66.47

Gene sequence:

>1035_bases
ATGTCGACGCAGATGATGAAGGCTGTTCAACAGCACGCCTTTGGCGGGCCGGAAGTCCTGAGCTATGAGGATGCGCCCAT
GCCGGTCCTGCAGGCCGGTGAAGTACTGGTACAGGTGTACGCGGTGGGCGTGAACCCGCCGGACAGCTATCTGCGCGATG
GCTATCAACAGCTGCCGCCGGAGTGGCGGCCCGAGGTCCGCTTTCCCTTGATCCTCGGGACCGATCTCTCCGGGGTGGTG
GTGGCGCGGGCGGATGACGTCCGGGAGGTGGCGGTCGGCGACGAAGTGTATGCTATGGCGCGTTTTCCCGAAGGGGCGGC
AGGGGGCAGCCGCGCTTACGCCGAGTACGTTAGCGTGCCGGTCAGCGACCTCGCTCGCAAACCCCTAACCCTGAGCCATC
AGCAGGCTGCCGCGGTGCCGATGTCGCTCCTGACCGCCTGGCAGTTCATGATCGATCCCGGGCATGAGGTGGCGAATCCG
CTGCAGCCGGGGCCGCATCGGCCGGTGCCGCTGGCGGGTAAACGCGTCCTGGTCAACGGCGCGGCAGGGGGAGTCGGTCA
TTTCGCCGTGCAGTTGGCGAAGTGGCAGGGCGCCGAGGTGATTGCCGTCGCCGCGGGCTGCCATGAAGCCTTTTTACGTC
AGCTCGGCGCGGATAGCGTTATCGACTATACAACCACCGCAGTGGAGGAGACGGTGCGTGACCTGGATCTGGTCATTGAT
GCGCCGGGCGGGCCGGCCAGCGGCCGTTTTTTGCGTACCCTGCGACCCGGCGGCGCGCTGTACCCGATCTTTCCGCTCGG
CTTTGCCGGGGCGGAAGAGGCGCGACAGCGCGGAGTGACGGTCTCCACCACCCAGGTACGCTCCAGCGGCGCGCAGCTCG
CCCGGCTTGCCGACCTGCTGGACGCCGGGGTCATCCGGGTTGCAATTGACAGCGTTTTCCCCCTTGCGCAGGCGCAGATG
GCGCATGAGCGAGCAGCGCAGGGGCATCTTGAGGGCAAAATCGTGCTGTCGGTGATGGACTCGTCAGCCGGGTAA

Upstream 100 bases:

>100_bases
ATCAGCTCTCATTGGCGTTCCTTGACAATCGGAGCACTGCTCCATATATTCGGAACAGTGCTCCGTTAAATATAACGCAT
TTCCATATGAGGAGAAAGCG

Downstream 100 bases:

>100_bases
TCCCCGACGGCCAGGGTTGCGTTTTTGCTTCTCCCGGCGGCGGACTAAAACAGCGCCAGCCGGGCAAGACGGTCCAGCGC
GTTGCTCAGCGCCTCGTCCC

Product: zinc-containing alcohol dehydrogenase superfamily protein

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSTQMMKAVQQHAFGGPEVLSYEDAPMPVLQAGEVLVQVYAVGVNPPDSYLRDGYQQLPPEWRPEVRFPLILGTDLSGVV
VARADDVREVAVGDEVYAMARFPEGAAGGSRAYAEYVSVPVSDLARKPLTLSHQQAAAVPMSLLTAWQFMIDPGHEVANP
LQPGPHRPVPLAGKRVLVNGAAGGVGHFAVQLAKWQGAEVIAVAAGCHEAFLRQLGADSVIDYTTTAVEETVRDLDLVID
APGGPASGRFLRTLRPGGALYPIFPLGFAGAEEARQRGVTVSTTQVRSSGAQLARLADLLDAGVIRVAIDSVFPLAQAQM
AHERAAQGHLEGKIVLSVMDSSAG

Sequences:

>Translated_344_residues
MSTQMMKAVQQHAFGGPEVLSYEDAPMPVLQAGEVLVQVYAVGVNPPDSYLRDGYQQLPPEWRPEVRFPLILGTDLSGVV
VARADDVREVAVGDEVYAMARFPEGAAGGSRAYAEYVSVPVSDLARKPLTLSHQQAAAVPMSLLTAWQFMIDPGHEVANP
LQPGPHRPVPLAGKRVLVNGAAGGVGHFAVQLAKWQGAEVIAVAAGCHEAFLRQLGADSVIDYTTTAVEETVRDLDLVID
APGGPASGRFLRTLRPGGALYPIFPLGFAGAEEARQRGVTVSTTQVRSSGAQLARLADLLDAGVIRVAIDSVFPLAQAQM
AHERAAQGHLEGKIVLSVMDSSAG
>Mature_343_residues
STQMMKAVQQHAFGGPEVLSYEDAPMPVLQAGEVLVQVYAVGVNPPDSYLRDGYQQLPPEWRPEVRFPLILGTDLSGVVV
ARADDVREVAVGDEVYAMARFPEGAAGGSRAYAEYVSVPVSDLARKPLTLSHQQAAAVPMSLLTAWQFMIDPGHEVANPL
QPGPHRPVPLAGKRVLVNGAAGGVGHFAVQLAKWQGAEVIAVAAGCHEAFLRQLGADSVIDYTTTAVEETVRDLDLVIDA
PGGPASGRFLRTLRPGGALYPIFPLGFAGAEEARQRGVTVSTTQVRSSGAQLARLADLLDAGVIRVAIDSVFPLAQAQMA
HERAAQGHLEGKIVLSVMDSSAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=374, Percent_Identity=32.8877005347594, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI194239674, Length=353, Percent_Identity=27.7620396600567, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI13236495, Length=353, Percent_Identity=27.7620396600567, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI194239676, Length=337, Percent_Identity=27.0029673590504, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI24308257, Length=264, Percent_Identity=32.1969696969697, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI18379349, Length=340, Percent_Identity=25.8823529411765, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI22538446, Length=368, Percent_Identity=28.2608695652174, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI22538444, Length=368, Percent_Identity=28.2608695652174, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790485, Length=361, Percent_Identity=26.5927977839335, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507255, Length=358, Percent_Identity=28.4916201117318, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71987554, Length=365, Percent_Identity=28.2191780821918, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6319520, Length=249, Percent_Identity=28.9156626506024, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319945, Length=239, Percent_Identity=28.8702928870293, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323493, Length=239, Percent_Identity=28.4518828451883, Blast_Score=66, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324195, Length=240, Percent_Identity=27.9166666666667, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24581339, Length=375, Percent_Identity=29.3333333333333, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24581341, Length=371, Percent_Identity=29.1105121293801, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 36356; Mature: 36225

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQMMKAVQQHAFGGPEVLSYEDAPMPVLQAGEVLVQVYAVGVNPPDSYLRDGYQQLPP
CCHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHEEEEEEECCCCCHHHHHCHHHHCCC
EWRPEVRFPLILGTDLSGVVVARADDVREVAVGDEVYAMARFPEGAAGGSRAYAEYVSVP
CCCCCCEEEEEECCCCCCEEEECCCCHHHHHCCCHHEEEEECCCCCCCCHHHHHHHHCCC
VSDLARKPLTLSHQQAAAVPMSLLTAWQFMIDPGHEVANPLQPGPHRPVPLAGKRVLVNG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCEEEEEC
AAGGVGHFAVQLAKWQGAEVIAVAAGCHEAFLRQLGADSVIDYTTTAVEETVRDLDLVID
CCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEE
APGGPASGRFLRTLRPGGALYPIFPLGFAGAEEARQRGVTVSTTQVRSSGAQLARLADLL
CCCCCCCCCEEEEECCCCCEEECCCCCCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHH
DAGVIRVAIDSVFPLAQAQMAHERAAQGHLEGKIVLSVMDSSAG
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
STQMMKAVQQHAFGGPEVLSYEDAPMPVLQAGEVLVQVYAVGVNPPDSYLRDGYQQLPP
CHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHEEEEEEECCCCCHHHHHCHHHHCCC
EWRPEVRFPLILGTDLSGVVVARADDVREVAVGDEVYAMARFPEGAAGGSRAYAEYVSVP
CCCCCCEEEEEECCCCCCEEEECCCCHHHHHCCCHHEEEEECCCCCCCCHHHHHHHHCCC
VSDLARKPLTLSHQQAAAVPMSLLTAWQFMIDPGHEVANPLQPGPHRPVPLAGKRVLVNG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCEEEEEC
AAGGVGHFAVQLAKWQGAEVIAVAAGCHEAFLRQLGADSVIDYTTTAVEETVRDLDLVID
CCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEE
APGGPASGRFLRTLRPGGALYPIFPLGFAGAEEARQRGVTVSTTQVRSSGAQLARLADLL
CCCCCCCCCEEEEECCCCCEEECCCCCCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHH
DAGVIRVAIDSVFPLAQAQMAHERAAQGHLEGKIVLSVMDSSAG
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]