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The map label for this gene is norB [H]

Identifier: 152969849

GI number: 152969849

Start: 1455370

End: 1456761

Strand: Direct

Name: norB [H]

Synonym: KPN_01295

Alternate gene names: 152969849

Gene position: 1455370-1456761 (Clockwise)

Preceding gene: 152969848

Following gene: 152969851

Centisome position: 27.38

GC content: 63.36

Gene sequence:

>1392_bases
ATGAGCACTGTCACAATAAGCGACACCCCGTTTGTCGGCAACGATCGCCTGCTGGTGGGGATTGTGTTAAGCGTTTTAAC
CTTCTGGCTGTTCGCCCAGTCGGTGATTAACGTGGTGCCGGCGATGAAAAGCAGCCTTGATATCTCTCTTGAAACCTTAA
CTCTGGCCGTCAGTCTCAGCGCGCTGTTCAGCGGCTGCTTCGTGGTCGCCAGCGGCGGCCTGGCGGACAAGTTTGGCCGG
ATGCGGATGACCACCCTCGGGCTGGGGCTGAGTATCGTCGGCAGCGCCATGCTGGTGGTCGCTCAGGGACCGGGGCTGTT
CCTCGCAGGCCGGGTGCTGCAGGGACTGTCGGCAGCCTGCATTATGCCGGCGACGCTGGCGCTGATTAAGACCTGGTATG
AGGGGCGTGCGCGGCAGCGGGCGGTCAGCTTCTGGGTCATCGGCTCGTGGGGCGGCAGCGGTCTCTGTTCCTTCGTTGGC
GGGGCTATCGCCACCGGACTAGGCTGGCGCTGGATCTTCGTCTTCTCGATCGCCGTTGCCCTGCTGGCGCTGTTTTTGCT
GCGCGGCACGCCGGAGAGCCGCAGCGCCAGCGCCAGCCAGCATAAGCTGGACGTCGGCGGTCTGCTGAGCCTGATTGTTG
TGCTGGTGCTGGTGAATCTGTTTATCAGCAAGGGCCACGGCTGGGGCTGGAGCAGCCCGCTGTCGTTGACCATGCTGGCC
GGGGCGCTGGCGGCGGGGACGATATTTATCCGCAACGGCATGCGCAAAGGCGAGGCGGCGCTGATTGATTTTGCGCTGTT
CCGCAATCGGGCCTACGGTGCGGCAGTGCTGTCGAACTTTCTGCTCAATGGGGCGATTGGCACCATGATGATCGCCAGCA
TCTGGCTGCAGCAAGGGCACCACCTGACGCCGCTGGAGAGCGGGATGATGACCCTCGGCTATCTGGTGACCGTCCTGGCG
ATGATCCGGGTCGGCGAGAAGCTGCTGCAGCGCTATGGCGCCCGGCTGCCGATGATGGCCGGTCCGGTGTTGACCGCTAT
CGCCATCGCGCTGATATCCTGCACCTTTCTCGAAAAGGCGCTGTATATCGGGGTGGTGTTCGCCAGCAACGTGCTGTTTG
GCCTCGGGCTGGGCTGCTACGCCACGCCATCGACGGATACCGCGGTGGCCAATGCCCCGGAGAACAAAATCGGCGTCGCC
TCGGGGATCTATAAGATGGGCAGCTCGCTGGGCGGGGCGATGGGTATCGCCGTGACAGCGTCACTTTTCGCGCTGTTTTT
ACCGCTGGGCATGGCCCATGCCGCCCAGTATGCCCTGTGGTTTAACGCCGTCTTATGTCTGGGGGCGATGGCGGTGAGCG
CGCTGCTGCTGCCGCGCGCCTCTCACAGCTGA

Upstream 100 bases:

>100_bases
TATGACTTCAACGACGCGCTGTTGCTGACCGGCGCGGCGCTGTGGTGTGGCCTGACGGAACGCTATCTGCGCTAATCACG
AGAAACGGGAGGGCCATGCG

Downstream 100 bases:

>100_bases
TGCTTTTTCAGCAGCGCGCGGAGCTGGTGATAGGTTAATCCCAGCAGCTCCGCCGCCTGCTTCTGATGATACTTCGCCTG
CTCCAGACTCCGCTGCAGCA

Product: putative transmembrane efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSTVTISDTPFVGNDRLLVGIVLSVLTFWLFAQSVINVVPAMKSSLDISLETLTLAVSLSALFSGCFVVASGGLADKFGR
MRMTTLGLGLSIVGSAMLVVAQGPGLFLAGRVLQGLSAACIMPATLALIKTWYEGRARQRAVSFWVIGSWGGSGLCSFVG
GAIATGLGWRWIFVFSIAVALLALFLLRGTPESRSASASQHKLDVGGLLSLIVVLVLVNLFISKGHGWGWSSPLSLTMLA
GALAAGTIFIRNGMRKGEAALIDFALFRNRAYGAAVLSNFLLNGAIGTMMIASIWLQQGHHLTPLESGMMTLGYLVTVLA
MIRVGEKLLQRYGARLPMMAGPVLTAIAIALISCTFLEKALYIGVVFASNVLFGLGLGCYATPSTDTAVANAPENKIGVA
SGIYKMGSSLGGAMGIAVTASLFALFLPLGMAHAAQYALWFNAVLCLGAMAVSALLLPRASHS

Sequences:

>Translated_463_residues
MSTVTISDTPFVGNDRLLVGIVLSVLTFWLFAQSVINVVPAMKSSLDISLETLTLAVSLSALFSGCFVVASGGLADKFGR
MRMTTLGLGLSIVGSAMLVVAQGPGLFLAGRVLQGLSAACIMPATLALIKTWYEGRARQRAVSFWVIGSWGGSGLCSFVG
GAIATGLGWRWIFVFSIAVALLALFLLRGTPESRSASASQHKLDVGGLLSLIVVLVLVNLFISKGHGWGWSSPLSLTMLA
GALAAGTIFIRNGMRKGEAALIDFALFRNRAYGAAVLSNFLLNGAIGTMMIASIWLQQGHHLTPLESGMMTLGYLVTVLA
MIRVGEKLLQRYGARLPMMAGPVLTAIAIALISCTFLEKALYIGVVFASNVLFGLGLGCYATPSTDTAVANAPENKIGVA
SGIYKMGSSLGGAMGIAVTASLFALFLPLGMAHAAQYALWFNAVLCLGAMAVSALLLPRASHS
>Mature_462_residues
STVTISDTPFVGNDRLLVGIVLSVLTFWLFAQSVINVVPAMKSSLDISLETLTLAVSLSALFSGCFVVASGGLADKFGRM
RMTTLGLGLSIVGSAMLVVAQGPGLFLAGRVLQGLSAACIMPATLALIKTWYEGRARQRAVSFWVIGSWGGSGLCSFVGG
AIATGLGWRWIFVFSIAVALLALFLLRGTPESRSASASQHKLDVGGLLSLIVVLVLVNLFISKGHGWGWSSPLSLTMLAG
ALAAGTIFIRNGMRKGEAALIDFALFRNRAYGAAVLSNFLLNGAIGTMMIASIWLQQGHHLTPLESGMMTLGYLVTVLAM
IRVGEKLLQRYGARLPMMAGPVLTAIAIALISCTFLEKALYIGVVFASNVLFGLGLGCYATPSTDTAVANAPENKIGVAS
GIYKMGSSLGGAMGIAVTASLFALFLPLGMAHAAQYALWFNAVLCLGAMAVSALLLPRASHS

Specific function: Multidrug efflux pump that acts independently of norA and is one of the factors that confers resistance against diverse quinolones and chemical compounds [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=440, Percent_Identity=22.5, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790195, Length=395, Percent_Identity=23.5443037974684, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789042, Length=427, Percent_Identity=20.8430913348946, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081983, Length=361, Percent_Identity=24.0997229916898, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788392, Length=413, Percent_Identity=23.9709443099274, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48363; Mature: 48232

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVTISDTPFVGNDRLLVGIVLSVLTFWLFAQSVINVVPAMKSSLDISLETLTLAVSLS
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALFSGCFVVASGGLADKFGRMRMTTLGLGLSIVGSAMLVVAQGPGLFLAGRVLQGLSAAC
HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCHHHH
IMPATLALIKTWYEGRARQRAVSFWVIGSWGGSGLCSFVGGAIATGLGWRWIFVFSIAVA
HHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLALFLLRGTPESRSASASQHKLDVGGLLSLIVVLVLVNLFISKGHGWGWSSPLSLTMLA
HHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
GALAAGTIFIRNGMRKGEAALIDFALFRNRAYGAAVLSNFLLNGAIGTMMIASIWLQQGH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HLTPLESGMMTLGYLVTVLAMIRVGEKLLQRYGARLPMMAGPVLTAIAIALISCTFLEKA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LYIGVVFASNVLFGLGLGCYATPSTDTAVANAPENKIGVASGIYKMGSSLGGAMGIAVTA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
SLFALFLPLGMAHAAQYALWFNAVLCLGAMAVSALLLPRASHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STVTISDTPFVGNDRLLVGIVLSVLTFWLFAQSVINVVPAMKSSLDISLETLTLAVSLS
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALFSGCFVVASGGLADKFGRMRMTTLGLGLSIVGSAMLVVAQGPGLFLAGRVLQGLSAAC
HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCHHHH
IMPATLALIKTWYEGRARQRAVSFWVIGSWGGSGLCSFVGGAIATGLGWRWIFVFSIAVA
HHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLALFLLRGTPESRSASASQHKLDVGGLLSLIVVLVLVNLFISKGHGWGWSSPLSLTMLA
HHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
GALAAGTIFIRNGMRKGEAALIDFALFRNRAYGAAVLSNFLLNGAIGTMMIASIWLQQGH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HLTPLESGMMTLGYLVTVLAMIRVGEKLLQRYGARLPMMAGPVLTAIAIALISCTFLEKA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LYIGVVFASNVLFGLGLGCYATPSTDTAVANAPENKIGVASGIYKMGSSLGGAMGIAVTA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
SLFALFLPLGMAHAAQYALWFNAVLCLGAMAVSALLLPRASHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA