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The map label for this gene is celF [H]

Identifier: 152969788

GI number: 152969788

Start: 1385004

End: 1386350

Strand: Direct

Name: celF [H]

Synonym: KPN_01234

Alternate gene names: 152969788

Gene position: 1385004-1386350 (Clockwise)

Preceding gene: 152969787

Following gene: 152969789

Centisome position: 26.06

GC content: 55.9

Gene sequence:

>1347_bases
ATGAGCCAGAAATTAAAAGTAGTCACTATTGGCGGCGGCAGCAGCTATACCCCGGAATTACTGGAAGGATTTCTGAAACG
TTATCATGAACTGCCGGTAAGCGAACTGTGGTTGGTAGACGTCGAAGAGGGCCAGGAGAAGCTCGATATTATCCACGCGC
TGTGCCAGCGGATGGTAGAAAAAGCCGGCGTGCCGATGAAAGTCTATAAAACGCTGGATCGCCGCGCAGCGCTGCAGGGC
GCAGATTTCGTCACCACCCAGCTGCGCGTGGGCCAGCTGAAGGCGCGTGAAAAAGATGAGCGCATCCCGCTGAGCCACGG
CTACCTGGGCCAGGAGACCAACGGGGCGGGCGGGTTGTTTAAAGGACTGCGCACCATCCCGGTGATTTTCGATATCGTGA
AAGATGTGCAGGAAATTTGCCCCGACGCATGGATCATCAACTTCACCAACCCGGCGGGCATGGTCACCGAGGCGGTCTAT
CGCCACACCAATTTCAAACGCTTTATCGGCGTGTGCAACATTCCTATCGGCATGAAGATGTTCATCACCGACGTGCTGCA
GCTGAGCCCGAGCGATGAGCTGAATATCGACCTGTTTGGTCTGAACCACCTGGTGTTCGTCCGCGACGTGCTGGTTAACG
GCGTCTCGCGCTTCGATGAACTGCTGGACGGCGTGGCCTCCGGCCGTCTGACCGCGAACTCGGTGAAAAACATCTTCGAC
CTGCCGTTTAGCGAAGGGCTGATCCGCTCCCTGCGCCTGATCCCATGCTCTTACCTGCTCTACTACTTTAAGCCAAAAGA
GATGCTGGCGATTGAGATGGGCGAGTACTACAAAGGCGGTGCGCGCGCCCAGGTGGTGCAGAAGGTGGAGAAACAGCTGT
TCGAGCTGTATAAAAACCCGGATCTTAACGTTAAGCCGAAGGAGCTGGAGCAGCGCGGCGGGGCCTATTACTCTGATGCC
GCTTGCGAAGTAATCAATGCCATCTATAACGACAAGCAGACTGAGCACTATGTGAATATCCCGCATCATGGCCATGTCGA
TAATATCCCGGCGGACTGGGCGGTAGAGATGAGCTGCACCTTAGGCCGCGACGGCGCGAAGCCGACGCCGCGCATTACTC
ATTTCGATGAGAAGGTGTTAGGGCTTATCTACACCATCAAGGGGTTCGAAGTGGCGGCCAGCCAGGCGGCGATCAGCGGT
GAGCTGAACGATGTCCTGTTGGCGCTGAACTTAAGCCCGCTGATCCATTCCGATCGTGACGCCGAACAGCTGGCGCGGGA
GATGATTCTGGCGCATGAAAAATGGCTGCCGAACTTTGCGGCGACGATCGAAAAACTAAAATCGTAA

Upstream 100 bases:

>100_bases
CGTTCGCCGCCAGGTTTATACCGAAAATCATTCAAGTTAACTGTCCGCATTGTTGCGGAGAGCGGCCTGCGTCCAGGCGC
AGCGCCTAAGGGAGAAAAAG

Downstream 100 bases:

>100_bases
CGGGAGTAGCAAATGGAACGCGTATTGATCGTCAATGCCGACGACTTCGGCCTCAGTAAAGGGCAGAACTACGGCATTAT
CGAGGCCTGTCGCAACGGCG

Product: phospho-beta-glucosidase, NAD(P)-binding (cellobiose-6-phosphate hydrolase)

Products: NA

Alternate protein names: Cellobiose-6-phosphate hydrolase; Phospho-chitobiase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSQKLKVVTIGGGSSYTPELLEGFLKRYHELPVSELWLVDVEEGQEKLDIIHALCQRMVEKAGVPMKVYKTLDRRAALQG
ADFVTTQLRVGQLKAREKDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVQEICPDAWIINFTNPAGMVTEAVY
RHTNFKRFIGVCNIPIGMKMFITDVLQLSPSDELNIDLFGLNHLVFVRDVLVNGVSRFDELLDGVASGRLTANSVKNIFD
LPFSEGLIRSLRLIPCSYLLYYFKPKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPDLNVKPKELEQRGGAYYSDA
ACEVINAIYNDKQTEHYVNIPHHGHVDNIPADWAVEMSCTLGRDGAKPTPRITHFDEKVLGLIYTIKGFEVAASQAAISG
ELNDVLLALNLSPLIHSDRDAEQLAREMILAHEKWLPNFAATIEKLKS

Sequences:

>Translated_448_residues
MSQKLKVVTIGGGSSYTPELLEGFLKRYHELPVSELWLVDVEEGQEKLDIIHALCQRMVEKAGVPMKVYKTLDRRAALQG
ADFVTTQLRVGQLKAREKDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVQEICPDAWIINFTNPAGMVTEAVY
RHTNFKRFIGVCNIPIGMKMFITDVLQLSPSDELNIDLFGLNHLVFVRDVLVNGVSRFDELLDGVASGRLTANSVKNIFD
LPFSEGLIRSLRLIPCSYLLYYFKPKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPDLNVKPKELEQRGGAYYSDA
ACEVINAIYNDKQTEHYVNIPHHGHVDNIPADWAVEMSCTLGRDGAKPTPRITHFDEKVLGLIYTIKGFEVAASQAAISG
ELNDVLLALNLSPLIHSDRDAEQLAREMILAHEKWLPNFAATIEKLKS
>Mature_447_residues
SQKLKVVTIGGGSSYTPELLEGFLKRYHELPVSELWLVDVEEGQEKLDIIHALCQRMVEKAGVPMKVYKTLDRRAALQGA
DFVTTQLRVGQLKAREKDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVQEICPDAWIINFTNPAGMVTEAVYR
HTNFKRFIGVCNIPIGMKMFITDVLQLSPSDELNIDLFGLNHLVFVRDVLVNGVSRFDELLDGVASGRLTANSVKNIFDL
PFSEGLIRSLRLIPCSYLLYYFKPKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPDLNVKPKELEQRGGAYYSDAA
CEVINAIYNDKQTEHYVNIPHHGHVDNIPADWAVEMSCTLGRDGAKPTPRITHFDEKVLGLIYTIKGFEVAASQAAISGE
LNDVLLALNLSPLIHSDRDAEQLAREMILAHEKWLPNFAATIEKLKS

Specific function: Hydrolyzes a wide variety of P-beta-glucosides including cellobiose-6P, salicin-6P, arbutin-6P, gentiobiose-6P, methyl- beta-glucoside-6P and p-nitrophenyl-beta-D-glucopyranoside-6P. Is also able to hydrolyze phospho-N,N'-diacetylchitobiose [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=447, Percent_Identity=86.5771812080537, Blast_Score=814, Evalue=0.0,
Organism=Escherichia coli, GI1790560, Length=468, Percent_Identity=23.7179487179487, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 50250; Mature: 50118

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKLKVVTIGGGSSYTPELLEGFLKRYHELPVSELWLVDVEEGQEKLDIIHALCQRMVE
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHEEEEEHHCCHHHHHHHHHHHHHHHH
KAGVPMKVYKTLDRRAALQGADFVTTQLRVGQLKAREKDERIPLSHGYLGQETNGAGGLF
HCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
KGLRTIPVIFDIVKDVQEICPDAWIINFTNPAGMVTEAVYRHTNFKRFIGVCNIPIGMKM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCHHHHHEECCCCCCHHH
FITDVLQLSPSDELNIDLFGLNHLVFVRDVLVNGVSRFDELLDGVASGRLTANSVKNIFD
HHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
LPFSEGLIRSLRLIPCSYLLYYFKPKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNP
CCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHHHHCCCHHHHHHHHHHHHHHHHHCCC
DLNVKPKELEQRGGAYYSDAACEVINAIYNDKQTEHYVNIPHHGHVDNIPADWAVEMSCT
CCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEEEE
LGRDGAKPTPRITHFDEKVLGLIYTIKGFEVAASQAAISGELNDVLLALNLSPLIHSDRD
ECCCCCCCCCCCCCHHHHHHHHHHEECCCHHHHHHHHHCCCCCCEEEEEECCHHHCCCCC
AEQLAREMILAHEKWLPNFAATIEKLKS
HHHHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
SQKLKVVTIGGGSSYTPELLEGFLKRYHELPVSELWLVDVEEGQEKLDIIHALCQRMVE
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHEEEEEHHCCHHHHHHHHHHHHHHHH
KAGVPMKVYKTLDRRAALQGADFVTTQLRVGQLKAREKDERIPLSHGYLGQETNGAGGLF
HCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
KGLRTIPVIFDIVKDVQEICPDAWIINFTNPAGMVTEAVYRHTNFKRFIGVCNIPIGMKM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCHHHHHEECCCCCCHHH
FITDVLQLSPSDELNIDLFGLNHLVFVRDVLVNGVSRFDELLDGVASGRLTANSVKNIFD
HHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
LPFSEGLIRSLRLIPCSYLLYYFKPKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNP
CCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHHHHHCCCHHHHHHHHHHHHHHHHHCCC
DLNVKPKELEQRGGAYYSDAACEVINAIYNDKQTEHYVNIPHHGHVDNIPADWAVEMSCT
CCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEEEE
LGRDGAKPTPRITHFDEKVLGLIYTIKGFEVAASQAAISGELNDVLLALNLSPLIHSDRD
ECCCCCCCCCCCCCHHHHHHHHHHEECCCHHHHHHHHHCCCCCCEEEEEECCHHHCCCCC
AEQLAREMILAHEKWLPNFAATIEKLKS
HHHHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2179047; 9097039; 9278503; 8121401; 10572139; 9405618; 10913117 [H]